(Circular) (Six-base) MAPSORT of: pFN21AB8693.seq Check: 1872 from: 1 to: 6319 pFN21AB8693 6319 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2535 4129 278 Size: 53 83 186 936 999 1594 2468 Fragments arranged by size: 2468 1594 999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2043 2957 3098 3248 4382 5766 6034 1632 Size: 411 914 141 150 1134 1384 268 1917 Fragments arranged by size: 1917 1384 1134 914 411 268 150 141 AfeI AGC'GCT Cuts at: 2717 2717 Size: 6319 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 2310 3069 3135 5218 6195 1203 Size: 1107 759 66 2083 977 1327 Fragments arranged by size: 2083 1327 1107 977 759 66 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 2390 5048 2390 Size: 2658 3661 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AlwNI CAG_nnn'CTG Cuts at: 2083 4062 5634 2083 Size: 1979 1572 2768 Fragments arranged by size: 2768 1979 1572 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 2762 3628 3854 4006 6115 1493 Size: 1269 866 226 152 2109 1697 Fragments arranged by size: 2109 1697 1269 866 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2016 3258 3644 1870 Size: 73 73 1242 386 4545 Fragments arranged by size: 4545 1242 386 73 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3548 3640 5095 618 Size: 325 217 627 1761 92 1455 1842 Fragments arranged by size: 1842 1761 1455 627 325 217 92 BanII G_rGCy'C Cuts at: 1391 2808 3063 3638 1391 Size: 1417 255 575 4072 Fragments arranged by size: 4072 1417 575 255 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3236 3288 928 Size: 971 1337 52 3959 Fragments arranged by size: 3959 1337 971 52 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2760 4211 5421 2760 Size: 1451 1210 3658 Fragments arranged by size: 3658 1451 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2444 3494 4930 Size: 107 122 71 631 605 772 1050 1436 Cuts at: 4930 136 Size: 1525 Fragments arranged by size: 1525 1436 1050 772 631 605 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 3152 3484 3736 3152 Size: 332 252 5735 Fragments arranged by size: 5735 332 252 Bme1580I G_kGCm'C Cuts at: 1790 3135 4380 5536 1790 Size: 1345 1245 1156 2573 Fragments arranged by size: 2573 1345 1245 1156 BmgBI CAC'GTC Cuts at: 1318 3072 1318 Size: 1754 4565 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2393 3384 3531 Size: 416 53 83 399 24 976 991 147 Cuts at: 3531 4050 5008 442 Size: 519 958 1753 Fragments arranged by size: 1753 991 976 958 519 416 399 147 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 3235 3499 2128 Size: 1107 264 4948 Fragments arranged by size: 4948 1107 264 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2210 2300 2908 3587 4979 1239 Size: 884 87 90 608 679 1392 2579 Fragments arranged by size: 2579 1392 884 679 608 90 87 Bpu10I CC'TnA_GC Cuts at: 2473 2473 Size: 6319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4444 5309 5607 4444 Size: 865 298 5156 Fragments arranged by size: 5156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4982 882 Size: 617 3483 2219 Fragments arranged by size: 3483 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4110 493 Size: 711 2906 2702 Fragments arranged by size: 2906 2702 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2532 4126 4508 5143 Size: 53 83 186 936 999 1594 382 635 Cuts at: 5143 275 Size: 1451 Fragments arranged by size: 1594 1451 999 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3545 3594 4752 5150 5424 5571 5982 3545 Size: 49 1158 398 274 147 411 3882 Fragments arranged by size: 3882 1158 411 398 274 147 49 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3232 3262 1178 Size: 30 165 30 1829 30 4235 Fragments arranged by size: 4235 1829 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2555 2680 2726 3179 3227 3253 1186 Size: 934 435 125 46 453 48 26 4252 Fragments arranged by size: 4252 934 453 435 125 48 46 26 BseYI C'CCAG_C Cuts at: 2083 2712 3003 3409 5522 6018 2083 Size: 629 291 406 2113 496 2384 Fragments arranged by size: 2384 2113 629 496 406 291 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2398 2523 1163 Size: 573 662 125 4959 Fragments arranged by size: 4959 662 573 125 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2554 4380 4465 5536 2554 Size: 1826 85 1071 3337 Fragments arranged by size: 3337 1826 1071 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 BsmBI CGTCTCn'nnnn_ Cuts at: 3292 3292 Size: 6319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2554 2808 3063 3135 3638 4380 4465 Size: 399 764 254 255 72 503 742 85 Cuts at: 4465 5536 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 764 742 503 399 255 254 85 72 BspEI T'CCGG_A Cuts at: 3594 5982 3594 Size: 2388 3931 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2421 3674 844 Size: 1577 1253 3489 Fragments arranged by size: 3489 1577 1253 BsrBI CCG'CTC Cuts at: 1394 1823 4206 1394 Size: 429 2383 3507 Fragments arranged by size: 3507 2383 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3616 4814 4988 65 Size: 1183 2368 1198 174 1396 Fragments arranged by size: 2368 1396 1198 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2150 4963 5150 1700 Size: 110 199 141 2813 187 2869 Fragments arranged by size: 2869 2813 199 187 141 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 2510 3478 794 Size: 1716 968 3635 Fragments arranged by size: 3635 1716 968 BstBI TT'CG_AA Cuts at: 2557 5123 2557 Size: 2566 3753 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstYI r'GATC_y Cuts at: 1972 2908 3090 3649 4016 4401 4418 5870 5956 Size: 936 182 559 367 385 17 1452 86 Cuts at: 5956 5968 6314 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 936 559 385 367 346 182 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2076 2076 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 2004 3310 3396 3580 513 Size: 552 939 1306 86 184 3252 Fragments arranged by size: 3252 1306 939 552 184 86 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 3835 4629 4649 3835 Size: 794 20 5505 Fragments arranged by size: 5505 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3310 3345 529 Size: 35 2746 35 3503 Fragments arranged by size: 3503 2746 35 35 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DraIII CAC_nnn'GTG Cuts at: 2272 2272 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2200 2251 Size: 54 1425 50 275 18 177 193 51 Cuts at: 2251 2436 3766 4655 6284 8 Size: 185 1330 889 1629 43 Fragments arranged by size: 1629 1425 1330 889 275 193 185 177 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2062 2174 2559 3026 3131 3239 4249 1353 Size: 709 112 385 467 105 108 1010 3423 Fragments arranged by size: 3423 1010 709 467 385 112 108 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2123 2210 2300 2908 2957 3098 Size: 393 411 80 87 90 608 49 141 Cuts at: 3098 3248 3587 4382 4979 5766 6034 1239 Size: 150 339 795 597 787 268 1524 Fragments arranged by size: 1524 795 787 608 597 411 393 339 268 150 141 90 87 80 49 EcoNI CCTnn'n_nnAGG Cuts at: 2411 2411 Size: 6319 EcoO109I rG'GnC_Cy Cuts at: 2046 3559 2046 Size: 1513 4806 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 EcoRV GAT'ATC Cuts at: 2245 2245 Size: 6319 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2016 2719 3422 5466 1678 Size: 338 703 703 2044 2531 Fragments arranged by size: 2531 2044 703 703 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2376 2408 2857 2881 2889 Size: 32 135 32 804 32 449 24 8 Cuts at: 2889 2913 4960 4992 5034 5066 1373 Size: 24 2047 32 42 32 2626 Fragments arranged by size: 2626 2047 804 449 135 42 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2959 3663 3909 669 Size: 988 281 1021 704 246 3079 Fragments arranged by size: 3079 1021 988 704 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 2959 3909 2959 Size: 950 5369 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3568 5433 5617 1305 Size: 2263 1865 184 2007 Fragments arranged by size: 2263 2007 1865 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2253 10 Size: 54 1425 343 421 4076 Fragments arranged by size: 4076 1425 421 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3290 4277 4636 4795 518 Size: 648 2124 987 359 159 2042 Fragments arranged by size: 2124 2042 987 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2145 2976 3735 4412 5560 5805 1606 Size: 427 112 831 759 677 1148 245 2120 Fragments arranged by size: 2120 1148 831 759 677 427 245 112 NaeI GCC'GGC Cuts at: 2011 2011 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4828 Fragments arranged by size: 4828 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 2009 2009 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 3139 3295 3677 5222 1132 Size: 97 1910 156 382 1545 2229 Fragments arranged by size: 2229 1910 1545 382 156 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 3135 5218 3135 Size: 2083 4236 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6319 PfoI T'CCnGG_A Cuts at: 2114 2277 2114 Size: 163 6156 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2046 2046 Size: 6319 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PstI C_TGCA'G Cuts at: 830 3474 3671 830 Size: 2644 197 3478 Fragments arranged by size: 3478 2644 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SapI GCTCTTCn'nnn_ Cuts at: 2559 2559 Size: 6319 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SfcI C'TryA_G Cuts at: 826 1045 3470 3667 4802 5483 5674 826 Size: 219 2425 197 1135 681 191 1471 Fragments arranged by size: 2425 1471 1135 681 219 197 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3260 3646 1872 Size: 1388 386 4545 Fragments arranged by size: 4545 1388 386 SmlI C'TyrA_G Cuts at: 820 1017 1943 4423 5324 5586 820 Size: 197 926 2480 901 262 1553 Fragments arranged by size: 2480 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3295 3677 1132 Size: 2163 382 3774 Fragments arranged by size: 3774 2163 382 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2004 2133 3328 3615 3758 513 Size: 552 939 129 1195 287 143 3074 Fragments arranged by size: 3074 1195 939 552 287 143 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4346 4363 4516 4701 5146 325 Size: 1255 2766 17 153 185 445 1498 Fragments arranged by size: 2766 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4565 6277 96 Size: 274 80 33 51 1443 2588 1712 138 Fragments arranged by size: 2588 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 3258 3644 1870 Size: 1388 386 4545 Fragments arranged by size: 4545 1388 386 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2533 4127 276 Size: 53 83 186 936 999 1594 2468 Fragments arranged by size: 2468 1594 999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI