(Circular) (Six-base) MAPSORT of: pFN21AB8684.seq Check: 4092 from: 1 to: 5842 pFN21AB8684 5842 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3652 278 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 1937 Size: 1248 4594 Acc65I G'GTAC_C Cuts at: 3163 3163 Size: 5842 AclI AA'CG_TT Cuts at: 1563 2322 3969 4342 1563 Size: 759 1647 373 3063 Fragments arranged by size: 3063 1647 759 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2439 3905 5289 5557 1632 Size: 807 1466 1384 268 1917 Fragments arranged by size: 1917 1466 1384 807 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5645 AflIII A'CryG_T Cuts at: 1203 2797 4741 5718 1203 Size: 1594 1944 977 1327 Fragments arranged by size: 1944 1594 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 2270 4571 2270 Size: 2301 3541 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2325 1184 Size: 1141 4701 AlwNI CAG_nnn'CTG Cuts at: 2085 3585 5157 2085 Size: 1500 1572 2770 Fragments arranged by size: 2770 1572 1500 ApaLI G'TGCA_C Cuts at: 3899 5055 3899 Size: 1156 4686 ApoI r'AATT_y Cuts at: 1493 2342 2927 3151 3377 3529 5638 1493 Size: 849 585 224 226 152 2109 1697 Fragments arranged by size: 2109 1697 849 585 226 224 152 AseI AT'TA_AT Cuts at: 160 1032 2255 4396 160 Size: 872 1223 2141 1606 Fragments arranged by size: 2141 1606 1223 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 3167 1870 Size: 73 1224 4545 Fragments arranged by size: 4545 1224 73 BamHI G'GATC_C Cuts at: 3172 3539 3172 Size: 367 5475 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3163 4618 618 Size: 325 217 627 1376 1455 1842 Fragments arranged by size: 1842 1455 1376 627 325 217 BanII G_rGCy'C Cuts at: 1391 3161 1391 Size: 1770 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2907 928 Size: 971 1008 3863 Fragments arranged by size: 3863 1008 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4033 4067 1903 Size: 34 2096 34 3678 Fragments arranged by size: 3678 2096 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3734 4944 3734 Size: 1210 4632 BclI T'GATC_A Cuts at: 1548 1548 Size: 5842 BfrBI ATG'CAT Cuts at: 2027 2157 2027 Size: 130 5712 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4453 136 Size: 107 122 71 631 605 2781 1525 Fragments arranged by size: 2781 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5837 5837 Size: 5842 BlpI GC'TnA_GC Cuts at: 3259 3259 Size: 5842 Bme1580I G_kGCm'C Cuts at: 1790 3903 5059 1790 Size: 2113 1156 2573 Fragments arranged by size: 2573 2113 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2468 3573 4531 Size: 416 53 83 399 24 1051 1105 958 Cuts at: 4531 442 Size: 1753 Fragments arranged by size: 1753 1105 1051 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4502 1239 Size: 3263 2579 Bpu10I CC'TnA_GC Cuts at: 2999 2999 Size: 5842 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2632 2717 3967 4832 5130 2632 Size: 85 1250 865 298 3344 Fragments arranged by size: 3344 1250 865 298 85 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2257 4505 882 Size: 617 758 2248 2219 Fragments arranged by size: 2248 2219 758 617 BsaAI yAC'GTr Cuts at: 493 1204 2149 3633 493 Size: 711 945 1484 2702 Fragments arranged by size: 2702 1484 945 711 BsaBI GATnn'nnATC Cuts at: 3042 5836 3042 Size: 2794 3048 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2532 3649 4031 4666 Size: 53 83 186 936 999 1117 382 635 Cuts at: 4666 275 Size: 1451 Fragments arranged by size: 1451 1117 999 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4275 4673 4947 5094 5505 4275 Size: 398 274 147 411 4612 Fragments arranged by size: 4612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2451 2481 1178 Size: 30 165 30 1048 30 4539 Fragments arranged by size: 4539 1048 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2761 1186 Size: 1575 4267 BseYI C'CCAG_C Cuts at: 5045 5541 5045 Size: 496 5346 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5269 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3292 4053 5081 1540 Size: 275 186 1291 761 1028 2301 Fragments arranged by size: 2301 1291 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3903 3988 5059 3903 Size: 85 1071 4686 Fragments arranged by size: 4686 1071 85 BsmI GAATG_Cn' Cuts at: 2096 3353 3446 2096 Size: 1257 93 4492 Fragments arranged by size: 4492 1257 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3161 3903 3988 5059 1391 Size: 399 1371 742 85 1071 2174 Fragments arranged by size: 2174 1371 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5505 5505 Size: 5842 BspHI T'CATG_A Cuts at: 3731 5461 3731 Size: 1730 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3197 844 Size: 2353 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3729 1394 Size: 429 1906 3507 Fragments arranged by size: 3507 1906 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2029 4337 4511 65 Size: 1183 781 2308 174 1396 Fragments arranged by size: 2308 1396 1183 781 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4486 4673 1700 Size: 110 2676 187 2869 Fragments arranged by size: 2869 2676 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5842 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 3902 4914 3902 Size: 1012 4830 BstAPI GCAn_nnn'nTGC Cuts at: 794 2109 794 Size: 1315 4527 BstBI TT'CG_AA Cuts at: 4646 4646 Size: 5842 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5842 BstYI r'GATC_y Cuts at: 1972 3050 3172 3539 3924 3941 5393 5479 5491 Size: 1078 122 367 385 17 1452 86 12 Cuts at: 5491 5837 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1078 385 367 346 122 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2721 2883 2721 Size: 162 5680 BtgI C'CryG_G Cuts at: 513 1065 2004 2535 513 Size: 552 939 531 3820 Fragments arranged by size: 3820 939 552 531 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2145 487 Size: 683 56 919 4184 Fragments arranged by size: 4184 919 683 56 BtsI GCAGTG_nn' Cuts at: 3358 4152 4172 3358 Size: 794 20 5028 Fragments arranged by size: 5028 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2011 2046 529 Size: 35 1447 35 4325 Fragments arranged by size: 4325 1447 35 35 DraI TTT'AAA Cuts at: 2370 3146 2370 Size: 776 5066 DraIII CAC_nnn'GTG Cuts at: 2476 2476 Size: 5842 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2462 3289 4178 Size: 54 1425 50 275 18 632 827 889 Cuts at: 4178 5807 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 827 632 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3289 1537 Size: 275 1477 4090 Fragments arranged by size: 4090 1477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2738 2784 2807 3051 3772 1353 Size: 1385 46 23 244 721 3423 Fragments arranged by size: 3423 1385 721 244 46 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2904 4429 4803 4949 387 Size: 1064 138 1315 1525 374 146 1280 Fragments arranged by size: 1525 1315 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2439 3905 4502 5289 5557 1239 Size: 393 807 1466 597 787 268 1524 Fragments arranged by size: 1524 1466 807 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2919 2919 Size: 5842 EcoO109I rG'GnC_Cy Cuts at: 2968 2968 Size: 5842 EcoRI G'AATT_C Cuts at: 3151 3529 3151 Size: 378 5464 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3149 3149 Size: 5842 FspI TGC'GCA Cuts at: 4348 4348 Size: 5842 HaeII r_GCGC'y Cuts at: 1678 2781 4989 1678 Size: 1103 2208 2531 Fragments arranged by size: 2531 2208 1103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2256 2288 4483 4515 4557 Size: 32 135 32 684 32 2195 32 42 Cuts at: 4557 4589 1373 Size: 32 2626 Fragments arranged by size: 2626 2195 684 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3186 3432 669 Size: 988 281 1248 246 3079 Fragments arranged by size: 3079 1248 988 281 246 HindIII A'AGCT_T Cuts at: 748 2421 3131 748 Size: 1673 710 3459 Fragments arranged by size: 3459 1673 710 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KpnI G_GTAC'C Cuts at: 3167 3167 Size: 5842 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 2797 5718 2797 Size: 2921 2921 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2451 4956 5140 1305 Size: 1146 2505 184 2007 Fragments arranged by size: 2505 2007 1146 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4020 Fragments arranged by size: 4020 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3800 4159 4318 518 Size: 648 2634 359 159 2042 Fragments arranged by size: 2634 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3258 3935 5083 5328 1606 Size: 1652 677 1148 245 2120 Fragments arranged by size: 2120 1652 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4351 Fragments arranged by size: 4351 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 3289 3289 Size: 5842 NsiI A_TGCA'T Cuts at: 2029 2159 2029 Size: 130 5712 NspI r_CATG'y Cuts at: 1132 1229 2022 2031 2069 2492 3200 4745 1132 Size: 97 793 9 38 423 708 1545 2229 Fragments arranged by size: 2229 1545 793 708 423 97 38 9 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 2476 1108 Size: 1368 4474 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PmlI CAC'GTG Cuts at: 2149 2149 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 5424 5456 3911 Size: 32 1481 32 4297 Fragments arranged by size: 4297 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PstI C_TGCA'G Cuts at: 830 2740 3194 830 Size: 1910 454 3478 Fragments arranged by size: 3478 1910 454 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 3023 4090 5802 3023 Size: 1067 1712 3063 Fragments arranged by size: 3063 1712 1067 SexAI A'CCwGG_T Cuts at: 2397 2397 Size: 5842 SfcI C'TryA_G Cuts at: 826 1045 2736 3190 4325 5006 5197 826 Size: 219 1691 454 1135 681 191 1471 Fragments arranged by size: 1691 1471 1135 681 454 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 3169 1872 Size: 1297 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2647 2696 3946 4847 5109 820 Size: 197 926 704 49 1250 901 262 1553 Fragments arranged by size: 1553 1250 926 901 704 262 197 49 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5842 StyI C'CwwG_G Cuts at: 513 1065 2004 2505 3281 513 Size: 552 939 501 776 3074 Fragments arranged by size: 3074 939 776 552 501 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2487 3869 3886 4039 4224 4669 325 Size: 1255 907 1382 17 153 185 445 1498 Fragments arranged by size: 1498 1382 1255 907 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3021 4088 5800 Size: 274 80 33 51 1443 1044 1067 1712 Cuts at: 5800 96 Size: 138 Fragments arranged by size: 1712 1443 1067 1044 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2387 2419 2494 2526 5802 5834 2387 Size: 32 75 32 3276 32 2395 Fragments arranged by size: 3276 2395 75 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3125 1316 Size: 1809 4033 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5842 XmaI C'CCGG_G Cuts at: 1870 3167 1870 Size: 1297 4545 XmnI GAAnn'nnTTC Cuts at: 2388 3971 2388 Size: 1583 4259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3650 276 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI