(Circular) (Six-base) MAPSORT of: pFN21AB8681.seq Check: 656 from: 1 to: 5836 pFN21AB8681 5836 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2250 2250 Size: 5836 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3090 3157 3090 Size: 67 5769 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3899 5283 5551 1632 Size: 2267 1384 268 1917 Fragments arranged by size: 2267 1917 1384 268 AfeI AGC'GCT Cuts at: 2270 2270 Size: 5836 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 2330 4735 5712 1203 Size: 1127 2405 977 1327 Fragments arranged by size: 2405 1327 1127 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 2089 2548 3579 5151 2089 Size: 459 1031 1572 2774 Fragments arranged by size: 2774 1572 1031 459 ApaLI G'TGCA_C Cuts at: 2732 3893 5049 2732 Size: 1161 1156 3519 Fragments arranged by size: 3519 1161 1156 ApoI r'AATT_y Cuts at: 1493 3145 3371 3523 5632 1493 Size: 1652 226 152 2109 1697 Fragments arranged by size: 2109 1697 1652 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2137 3161 1870 Size: 73 194 1024 4545 Fragments arranged by size: 4545 1024 194 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2238 2446 2828 2892 3090 Size: 325 217 627 451 208 382 64 198 Cuts at: 3090 3157 4612 618 Size: 67 1455 1842 Fragments arranged by size: 1842 1455 627 451 382 325 217 208 198 67 64 BanII G_rGCy'C Cuts at: 1391 2144 2171 2212 3155 1391 Size: 753 27 41 943 4072 Fragments arranged by size: 4072 943 753 41 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2354 2388 4027 4061 1903 Size: 34 417 34 1639 34 3678 Fragments arranged by size: 3678 1639 417 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BfrBI ATG'CAT Cuts at: 3050 3050 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2112 2582 4447 Size: 107 122 71 631 605 440 470 1865 Cuts at: 4447 136 Size: 1525 Fragments arranged by size: 1865 1525 631 605 470 440 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 2272 3253 2272 Size: 981 4855 Bme1580I G_kGCm'C Cuts at: 1790 2451 2736 3897 5053 1790 Size: 661 285 1161 1156 2573 Fragments arranged by size: 2573 1161 1156 661 285 BmgBI CAC'GTC Cuts at: 1318 2331 1318 Size: 1013 4823 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2378 2378 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2083 2885 4496 1239 Size: 844 802 1611 2579 Fragments arranged by size: 2579 1611 844 802 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2504 2867 3961 4826 5124 2504 Size: 363 1094 865 298 3216 Fragments arranged by size: 3216 1094 865 363 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2780 3643 4025 4660 Size: 53 83 186 936 1247 863 382 635 Cuts at: 4660 275 Size: 1451 Fragments arranged by size: 1451 1247 936 863 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2687 2717 2726 2756 1178 Size: 30 165 30 1284 30 9 30 4258 Fragments arranged by size: 4258 1284 165 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2422 2488 2708 1186 Size: 1236 66 220 4314 Fragments arranged by size: 4314 1236 220 66 BseYI C'CCAG_C Cuts at: 2068 2082 2179 2572 2658 3127 5039 5535 2068 Size: 14 97 393 86 469 1912 496 2369 Fragments arranged by size: 2369 1912 496 469 393 97 86 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2871 1163 Size: 573 1135 4128 Fragments arranged by size: 4128 1135 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2736 3897 3982 5053 2736 Size: 1161 85 1071 3519 Fragments arranged by size: 3519 1161 1071 85 BsmI GAATG_Cn' Cuts at: 3050 3347 3440 3050 Size: 297 93 5446 Fragments arranged by size: 5446 297 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2171 2212 2451 2736 3155 3897 Size: 399 354 27 41 239 285 419 742 Cuts at: 3897 3982 5053 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 419 399 354 285 239 85 41 27 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2250 3102 3191 844 Size: 1406 852 89 3489 Fragments arranged by size: 3489 1406 852 89 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4331 4505 65 Size: 1183 3083 174 1396 Fragments arranged by size: 3083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2967 4480 4667 1700 Size: 110 1157 1513 187 2869 Fragments arranged by size: 2869 1513 1157 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 2245 1925 Size: 320 5516 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5836 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 2608 1184 Size: 1424 4412 BstXI CCAn_nnnn'nTGG Cuts at: 2458 2845 2458 Size: 387 5449 BstYI r'GATC_y Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831 Size: 1194 367 385 17 1452 86 12 346 Cuts at: 5831 1972 Size: 1977 Fragments arranged by size: 1977 1452 1194 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2145 2171 2820 2832 513 Size: 552 939 141 26 649 12 3517 Fragments arranged by size: 3517 939 649 552 141 26 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2717 3352 4146 4166 2717 Size: 635 794 20 4387 Fragments arranged by size: 4387 794 635 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2501 2795 4843 809 Size: 507 1185 294 2048 1802 Fragments arranged by size: 2048 1802 1185 507 294 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2835 2856 3283 Size: 54 1425 50 275 18 1005 21 427 Cuts at: 3283 4172 5801 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1005 889 427 275 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2547 3766 1353 Size: 1194 1219 3423 Fragments arranged by size: 3423 1219 1194 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2732 4423 4797 4943 387 Size: 1064 138 1143 1691 374 146 1280 Fragments arranged by size: 1691 1280 1143 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 2885 3899 4496 5283 5551 1239 Size: 393 451 802 1014 597 787 268 1524 Fragments arranged by size: 1524 1014 802 787 597 451 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2927 2927 Size: 5836 EcoO109I rG'GnC_Cy Cuts at: 2254 2920 2254 Size: 666 5170 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FspI TGC'GCA Cuts at: 2411 4342 2411 Size: 1931 3905 HaeII r_GCGC'y Cuts at: 1678 2036 2195 2272 2517 4983 1678 Size: 358 159 77 245 2466 2531 Fragments arranged by size: 2531 2466 358 245 159 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373 Size: 32 135 32 2905 32 42 32 2626 Fragments arranged by size: 2905 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3094 3161 3094 Size: 67 5769 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2837 10 Size: 54 1425 343 1005 3009 Fragments arranged by size: 3009 1425 1005 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2456 3794 4153 4312 518 Size: 648 1290 1338 359 159 2042 Fragments arranged by size: 2042 1338 1290 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2173 2308 2855 3252 3929 5077 5322 Size: 483 84 135 547 397 677 1148 245 Cuts at: 5322 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 547 483 397 245 135 84 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NsiI A_TGCA'T Cuts at: 3052 3052 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 2200 2212 1348 Size: 852 12 4972 Fragments arranged by size: 4972 852 12 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2392 1108 Size: 1284 4552 PfoI T'CCnGG_A Cuts at: 2901 2901 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2411 2443 2795 2827 3905 3937 5418 5450 2411 Size: 32 352 32 1078 32 1481 32 2797 Fragments arranged by size: 2797 1481 1078 352 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2254 2920 2254 Size: 666 5170 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 2155 2307 2345 3188 830 Size: 1325 152 38 843 3478 Fragments arranged by size: 3478 1325 843 152 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2308 2308 Size: 5836 SacII CC_GC'GG Cuts at: 2174 2174 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 2630 4084 5796 2630 Size: 1454 1712 2670 Fragments arranged by size: 2670 1712 1454 SexAI A'CCwGG_T Cuts at: 2385 2529 2385 Size: 144 5692 SfcI C'TryA_G Cuts at: 826 1045 2151 2303 2341 2437 3184 4319 5000 Size: 219 1106 152 38 96 747 1135 681 Cuts at: 5000 5191 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1106 747 681 219 191 152 96 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2519 2882 3940 4841 5103 820 Size: 197 926 576 363 1058 901 262 1553 Fragments arranged by size: 1553 1058 926 901 576 363 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 3122 1517 Size: 1605 4231 StyI C'CwwG_G Cuts at: 513 1065 2004 2055 3275 513 Size: 552 939 51 1220 3074 Fragments arranged by size: 3074 1220 939 552 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2037 2627 2837 2935 3863 3880 4033 Size: 1255 457 590 210 98 928 17 153 Cuts at: 4033 4218 4663 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 928 590 457 445 210 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2628 2841 Size: 274 80 33 51 1443 345 306 213 Cuts at: 2841 4082 5794 96 Size: 1241 1712 138 Fragments arranged by size: 1712 1443 1241 345 306 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2687 2719 2726 2758 5796 5828 2687 Size: 32 7 32 3038 32 2695 Fragments arranged by size: 3038 2695 32 32 32 7 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2666 2773 2666 Size: 107 5729 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI