(Circular) (Six-base) MAPSORT of: pFN21AB8674.seq Check: 1171 from: 1 to: 6280 pFN21AB8674 6280 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3166 3166 Size: 6280 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4090 278 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3623 1937 Size: 1686 4594 Acc65I G'GTAC_C Cuts at: 3601 3601 Size: 6280 AclI AA'CG_TT Cuts at: 1563 4407 4780 1563 Size: 2844 373 3063 Fragments arranged by size: 3063 2844 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2571 2900 4343 5727 5995 1632 Size: 939 329 1443 1384 268 1917 Fragments arranged by size: 1917 1443 1384 939 329 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6083 AflIII A'CryG_T Cuts at: 1203 2182 5179 6156 1203 Size: 979 2997 977 1327 Fragments arranged by size: 2997 1327 979 977 AgeI A'CCGG_T Cuts at: 5111 5111 Size: 6280 AhdI GACnn_n'nnGTC Cuts at: 5009 5009 Size: 6280 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6280 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3470 3502 3470 Size: 32 6248 AlwNI CAG_nnn'CTG Cuts at: 2465 2864 3419 4023 5595 2465 Size: 399 555 604 1572 3150 Fragments arranged by size: 3150 1572 604 555 399 ApaLI G'TGCA_C Cuts at: 4337 5493 4337 Size: 1156 5124 ApoI r'AATT_y Cuts at: 1493 2360 2592 3310 3589 3815 3967 6076 1493 Size: 867 232 718 279 226 152 2109 1697 Fragments arranged by size: 2109 1697 867 718 279 232 226 152 AseI AT'TA_AT Cuts at: 160 1032 4834 160 Size: 872 3802 1606 Fragments arranged by size: 3802 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 AvaI C'yCGr_G Cuts at: 1870 1943 3605 1870 Size: 73 1662 4545 Fragments arranged by size: 4545 1662 73 AvrII C'CTAG_G Cuts at: 3262 3262 Size: 6280 BamHI G'GATC_C Cuts at: 3610 3977 3610 Size: 367 5913 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3601 5056 618 Size: 325 217 627 1814 1455 1842 Fragments arranged by size: 1842 1814 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2905 3599 1391 Size: 1514 694 4072 Fragments arranged by size: 4072 1514 694 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2514 928 Size: 971 615 4694 Fragments arranged by size: 4694 971 615 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4471 4505 1903 Size: 34 50 34 2450 34 3678 Fragments arranged by size: 3678 2450 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4172 5382 4172 Size: 1210 5070 BclI T'GATC_A Cuts at: 1548 1548 Size: 6280 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4891 136 Size: 107 122 71 631 605 3219 1525 Fragments arranged by size: 3219 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3290 6275 3290 Size: 2985 3295 BlpI GC'TnA_GC Cuts at: 3697 3697 Size: 6280 Bme1580I G_kGCm'C Cuts at: 1790 4341 5497 1790 Size: 2551 1156 2573 Fragments arranged by size: 2573 2551 1156 BmgBI CAC'GTC Cuts at: 1318 2041 1318 Size: 723 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4011 4969 442 Size: 416 53 83 399 24 2594 958 1753 Fragments arranged by size: 2594 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2421 3126 4940 1239 Size: 1182 705 1814 2579 Fragments arranged by size: 2579 1814 1182 705 Bpu10I CC'TnA_GC Cuts at: 2865 2865 Size: 6280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4405 5270 5568 4405 Size: 865 298 5117 Fragments arranged by size: 5117 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4943 882 Size: 617 3444 2219 Fragments arranged by size: 3444 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2285 4071 493 Size: 711 1081 1786 2702 Fragments arranged by size: 2702 1786 1081 711 BsaBI GATnn'nnATC Cuts at: 6274 6274 Size: 6280 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4087 4469 5104 275 Size: 53 83 186 936 2554 382 635 1451 Fragments arranged by size: 2554 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3514 4713 5111 5385 5532 5943 3514 Size: 1199 398 274 147 411 3851 Fragments arranged by size: 3851 1199 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2419 2449 1178 Size: 30 165 30 1016 30 5009 Fragments arranged by size: 5009 1016 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6280 BseYI C'CCAG_C Cuts at: 5483 5979 5483 Size: 496 5784 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5707 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3730 4491 5519 1540 Size: 275 186 1729 761 1028 2301 Fragments arranged by size: 2301 1729 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4341 4426 5497 4341 Size: 85 1071 5124 Fragments arranged by size: 5124 1071 85 BsmI GAATG_Cn' Cuts at: 3034 3228 3791 3884 3034 Size: 194 563 93 5430 Fragments arranged by size: 5430 563 194 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2905 3599 4341 4426 5497 1391 Size: 399 1115 694 742 85 1071 2174 Fragments arranged by size: 2174 1115 1071 742 694 399 85 BspEI T'CCGG_A Cuts at: 5943 5943 Size: 6280 BspHI T'CATG_A Cuts at: 4169 5899 4169 Size: 1730 4550 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2432 2899 3166 3635 844 Size: 1588 467 267 469 3489 Fragments arranged by size: 3489 1588 469 467 267 BsrBI CCG'CTC Cuts at: 1394 1823 4167 1394 Size: 429 2344 3507 Fragments arranged by size: 3507 2344 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4775 4949 65 Size: 1183 3527 174 1396 Fragments arranged by size: 3527 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4924 5111 1700 Size: 110 3114 187 2869 Fragments arranged by size: 3114 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6280 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6280 BssSI C'ACGA_G Cuts at: 4340 5352 4340 Size: 1012 5268 BstAPI GCAn_nnn'nTGC Cuts at: 794 3332 794 Size: 2538 3742 BstBI TT'CG_AA Cuts at: 5084 5084 Size: 6280 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6280 BstYI r'GATC_y Cuts at: 1972 2926 3290 3610 3977 4362 4379 5831 5917 Size: 954 364 320 367 385 17 1452 86 Cuts at: 5917 5929 6275 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 954 385 367 364 346 320 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5728 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5541 Fragments arranged by size: 5541 683 56 BtsI GCAGTG_nn' Cuts at: 3796 4590 4610 3796 Size: 794 20 5466 Fragments arranged by size: 5466 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6280 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6245 DraI TTT'AAA Cuts at: 2674 2784 3584 2674 Size: 110 800 5370 Fragments arranged by size: 5370 800 110 DraIII CAC_nnn'GTG Cuts at: 3206 3206 Size: 6280 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5287 809 Size: 507 3971 1802 Fragments arranged by size: 3971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3727 4616 6245 Size: 54 1425 50 275 18 1897 889 1629 Cuts at: 6245 8 Size: 43 Fragments arranged by size: 1897 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3727 1537 Size: 275 1915 4090 Fragments arranged by size: 4090 1915 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2211 4210 1353 Size: 858 1999 3423 Fragments arranged by size: 3423 1999 858 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4867 5241 5387 387 Size: 1064 138 3278 374 146 1280 Fragments arranged by size: 3278 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2421 2571 2900 3126 4343 4940 5727 Size: 393 789 150 329 226 1217 597 787 Cuts at: 5727 5995 1239 Size: 268 1524 Fragments arranged by size: 1524 1217 789 787 597 393 329 268 226 150 EcoNI CCTnn'n_nnAGG Cuts at: 2439 2439 Size: 6280 EcoO109I rG'GnC_Cy Cuts at: 2371 3266 2371 Size: 895 5385 EcoRI G'AATT_C Cuts at: 3310 3589 3967 3310 Size: 279 378 5623 Fragments arranged by size: 5623 378 279 EcoRV GAT'ATC Cuts at: 2391 2977 2391 Size: 586 5694 FspI TGC'GCA Cuts at: 4786 4786 Size: 6280 HaeII r_GCGC'y Cuts at: 1678 5427 1678 Size: 3749 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2911 2943 3452 3484 4921 Size: 32 135 32 1339 32 509 32 1437 Cuts at: 4921 4953 4995 5027 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1437 1339 509 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3624 3870 669 Size: 988 281 1686 246 3079 Fragments arranged by size: 3079 1686 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6280 HpaI GTT'AAC Cuts at: 3870 3870 Size: 6280 KpnI G_GTAC'C Cuts at: 3605 3605 Size: 6280 MfeI C'AATT_G Cuts at: 2716 3879 2716 Size: 1163 5117 MluI A'CGCG_T Cuts at: 6156 6156 Size: 6280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2092 2954 5394 5578 1305 Size: 787 862 2440 184 2007 Fragments arranged by size: 2440 2007 862 787 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4458 Fragments arranged by size: 4458 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4238 4597 4756 518 Size: 648 3072 359 159 2042 Fragments arranged by size: 3072 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2462 3696 4373 5521 5766 1606 Size: 856 1234 677 1148 245 2120 Fragments arranged by size: 2120 1234 1148 856 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5728 NdeI CA'TA_TG Cuts at: 387 2099 387 Size: 1712 4568 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6280 NotI GC'GGCC_GC Cuts at: 3727 3727 Size: 6280 NspI r_CATG'y Cuts at: 1132 1229 2186 3638 5183 1132 Size: 97 957 1452 1545 2229 Fragments arranged by size: 2229 1545 1452 957 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6280 PciI A'CATG_T Cuts at: 2182 5179 2182 Size: 2997 3283 PflMI CCAn_nnn'nTGG Cuts at: 1108 2721 2989 1108 Size: 1613 268 4399 Fragments arranged by size: 4399 1613 268 PfoI T'CCnGG_A Cuts at: 3066 3066 Size: 6280 PmeI GTTT'AAAC Cuts at: 3584 3584 Size: 6280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3383 3415 4349 4381 5862 5894 3383 Size: 32 934 32 1481 32 3769 Fragments arranged by size: 3769 1481 934 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2371 3266 2371 Size: 895 5385 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6280 PsiI TTA'TAA Cuts at: 3850 3850 Size: 6280 PstI C_TGCA'G Cuts at: 830 2910 3632 830 Size: 2080 722 3478 Fragments arranged by size: 3478 2080 722 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6280 PvuII CAG'CTG Cuts at: 2462 2462 Size: 6280 SalI G'TCGA_C Cuts at: 1936 3622 1936 Size: 1686 4594 SbfI CC_TGCA'GG Cuts at: 2910 3632 2910 Size: 722 5558 ScaI AGT'ACT Cuts at: 2119 4528 6240 2119 Size: 2409 1712 2159 Fragments arranged by size: 2409 2159 1712 SfcI C'TryA_G Cuts at: 826 1045 2070 2906 3256 3407 3628 4763 5444 Size: 219 1025 836 350 151 221 1135 681 Cuts at: 5444 5635 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1025 836 681 350 221 219 191 151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6280 SmaI CCC'GGG Cuts at: 1872 3607 1872 Size: 1735 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4384 5285 5547 820 Size: 197 926 2441 901 262 1553 Fragments arranged by size: 2441 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4071 493 Size: 3578 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6280 SphI G_CATG'C Cuts at: 1132 3638 1132 Size: 2506 3774 SspI AAT'ATT Cuts at: 5 52 4204 5 Size: 47 4152 2081 Fragments arranged by size: 4152 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6280 StyI C'CwwG_G Cuts at: 513 1065 3262 3719 513 Size: 552 2197 457 3074 Fragments arranged by size: 3074 2197 552 457 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4307 4324 4477 4662 5107 325 Size: 1255 2727 17 153 185 445 1498 Fragments arranged by size: 2727 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2061 2117 3114 Size: 274 80 33 51 1443 84 56 997 Cuts at: 3114 4526 6238 96 Size: 1412 1712 138 Fragments arranged by size: 1712 1443 1412 997 274 138 84 80 56 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6240 6272 6240 Size: 32 6248 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6280 XbaI T'CTAG_A Cuts at: 3616 3616 Size: 6280 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6280 XmaI C'CCGG_G Cuts at: 1870 3605 1870 Size: 1735 4545 XmnI GAAnn'nnTTC Cuts at: 2361 4409 2361 Size: 2048 4232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4088 276 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI