(Circular) (Six-base) MAPSORT of: pFN21AB8673.seq Check: 8329 from: 1 to: 6676 pFN21AB8673 6676 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4486 278 Size: 53 83 186 936 2950 2468 Fragments arranged by size: 2950 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4019 1937 Size: 2082 4594 Acc65I G'GTAC_C Cuts at: 3997 3997 Size: 6676 AclI AA'CG_TT Cuts at: 1563 4803 5176 1563 Size: 3240 373 3063 Fragments arranged by size: 3240 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2409 3172 3321 4739 6123 6391 1632 Size: 777 763 149 1418 1384 268 1917 Fragments arranged by size: 1917 1418 1384 777 763 268 149 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6479 AflIII A'CryG_T Cuts at: 1203 2958 5575 6552 1203 Size: 1755 2617 977 1327 Fragments arranged by size: 2617 1755 1327 977 AgeI A'CCGG_T Cuts at: 5507 5507 Size: 6676 AhdI GACnn_n'nnGTC Cuts at: 5405 5405 Size: 6676 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6676 AlwNI CAG_nnn'CTG Cuts at: 4419 5991 4419 Size: 1572 5104 ApaLI G'TGCA_C Cuts at: 4733 5889 4733 Size: 1156 5520 ApoI r'AATT_y Cuts at: 1493 2727 2930 2975 3047 3477 3985 4211 4363 Size: 1234 203 45 72 430 508 226 152 Cuts at: 4363 6472 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1234 508 430 226 203 152 72 45 AseI AT'TA_AT Cuts at: 160 1032 5230 160 Size: 872 4198 1606 Fragments arranged by size: 4198 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 AvaI C'yCGr_G Cuts at: 1870 1943 3086 3123 3651 4001 1870 Size: 73 1143 37 528 350 4545 Fragments arranged by size: 4545 1143 528 350 73 37 BamHI G'GATC_C Cuts at: 4006 4373 4006 Size: 367 6309 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3997 5452 618 Size: 325 217 627 2210 1455 1842 Fragments arranged by size: 2210 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2634 3995 1391 Size: 1243 1361 4072 Fragments arranged by size: 4072 1361 1243 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2120 928 Size: 971 221 5484 Fragments arranged by size: 5484 971 221 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4867 4901 1903 Size: 34 2930 34 3678 Fragments arranged by size: 3678 2930 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3093 4568 5778 3093 Size: 1475 1210 3991 Fragments arranged by size: 3991 1475 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6676 BfrBI ATG'CAT Cuts at: 3373 3373 Size: 6676 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5287 136 Size: 107 122 71 631 605 3615 1525 Fragments arranged by size: 3615 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2225 6671 2225 Size: 4446 2230 BlpI GC'TnA_GC Cuts at: 4093 4093 Size: 6676 Bme1580I G_kGCm'C Cuts at: 1790 4737 5893 1790 Size: 2947 1156 2573 Fragments arranged by size: 2947 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6676 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2495 4407 5365 Size: 416 53 83 399 24 1078 1912 958 Cuts at: 5365 442 Size: 1753 Fragments arranged by size: 1912 1753 1078 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6676 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2379 5336 1239 Size: 1140 2957 2579 Fragments arranged by size: 2957 2579 1140 Bpu10I CC'TnA_GC Cuts at: 2028 2028 Size: 6676 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4801 5666 5964 4801 Size: 865 298 5513 Fragments arranged by size: 5513 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3202 5339 882 Size: 617 1703 2137 2219 Fragments arranged by size: 2219 2137 1703 617 BsaAI yAC'GTr Cuts at: 493 1204 4467 493 Size: 711 3263 2702 Fragments arranged by size: 3263 2702 711 BsaBI GATnn'nnATC Cuts at: 6670 6670 Size: 6676 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4483 4865 5500 275 Size: 53 83 186 936 2950 382 635 1451 Fragments arranged by size: 2950 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2368 5109 5507 5781 5928 6339 2368 Size: 2741 398 274 147 411 2705 Fragments arranged by size: 2741 2705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2377 2407 3262 3292 1178 Size: 30 165 30 974 30 855 30 4562 Fragments arranged by size: 4562 974 855 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2049 3270 1186 Size: 863 1221 4592 Fragments arranged by size: 4592 1221 863 BseYI C'CCAG_C Cuts at: 5879 6375 5879 Size: 496 6180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2066 1163 Size: 573 330 5773 Fragments arranged by size: 5773 573 330 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4126 4887 5915 1540 Size: 275 186 2125 761 1028 2301 Fragments arranged by size: 2301 2125 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2634 2868 4737 4822 5893 2634 Size: 234 1869 85 1071 3417 Fragments arranged by size: 3417 1869 1071 234 85 BsmI GAATG_Cn' Cuts at: 2953 4187 4280 2953 Size: 1234 93 5349 Fragments arranged by size: 5349 1234 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2634 2868 3995 4737 4822 5893 1391 Size: 399 844 234 1127 742 85 1071 2174 Fragments arranged by size: 2174 1127 1071 844 742 399 234 85 BspEI T'CCGG_A Cuts at: 6339 6339 Size: 6676 BspHI T'CATG_A Cuts at: 4565 6295 4565 Size: 1730 4946 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4031 844 Size: 3187 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3222 4563 1394 Size: 429 1399 1341 3507 Fragments arranged by size: 3507 1399 1341 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3022 5171 5345 65 Size: 1183 1774 2149 174 1396 Fragments arranged by size: 2149 1774 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5320 5507 1700 Size: 110 3510 187 2869 Fragments arranged by size: 3510 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6676 BssSI C'ACGA_G Cuts at: 2669 4736 5748 2669 Size: 2067 1012 3597 Fragments arranged by size: 3597 2067 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6676 BstBI TT'CG_AA Cuts at: 5480 5480 Size: 6676 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6676 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2805 2011 Size: 794 5882 BstYI r'GATC_y Cuts at: 1972 2225 4006 4373 4758 4775 6227 6313 6325 Size: 253 1781 367 385 17 1452 86 12 Cuts at: 6325 6671 1972 Size: 346 1977 Fragments arranged by size: 1977 1781 1452 385 367 346 253 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2162 2162 Size: 6676 BtgI C'CryG_G Cuts at: 513 1065 2004 2283 513 Size: 552 939 279 4906 Fragments arranged by size: 4906 939 552 279 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5937 Fragments arranged by size: 5937 683 56 BtsI GCAGTG_nn' Cuts at: 3847 4192 4986 5006 3847 Size: 345 794 20 5517 Fragments arranged by size: 5517 794 345 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6676 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6641 DraI TTT'AAA Cuts at: 3469 3980 3469 Size: 511 6165 DraIII CAC_nnn'GTG Cuts at: 3260 3260 Size: 6676 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3653 5683 809 Size: 507 2337 2030 1802 Fragments arranged by size: 2337 2030 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3562 3768 4123 Size: 54 1425 50 275 18 1732 206 355 Cuts at: 4123 5012 6641 8 Size: 889 1629 43 Fragments arranged by size: 1732 1629 1425 889 355 275 206 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4123 1537 Size: 275 2311 4090 Fragments arranged by size: 4090 2311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2196 2646 3221 4606 1353 Size: 843 450 575 1385 3423 Fragments arranged by size: 3423 1385 843 575 450 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5263 5637 5783 387 Size: 1064 138 3674 374 146 1280 Fragments arranged by size: 3674 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2632 2632 Size: 6676 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2409 3172 3321 4739 5336 6123 Size: 393 747 30 763 149 1418 597 787 Cuts at: 6123 6391 1239 Size: 268 1524 Fragments arranged by size: 1524 1418 787 763 747 597 393 268 149 30 EcoO109I rG'GnC_Cy Cuts at: 3819 3819 Size: 6676 EcoRI G'AATT_C Cuts at: 3985 4363 3985 Size: 378 6298 FspI TGC'GCA Cuts at: 5182 5182 Size: 6676 HaeII r_GCGC'y Cuts at: 1678 5823 1678 Size: 4145 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2812 2844 3067 3099 5317 Size: 32 135 32 1240 32 223 32 2218 Cuts at: 5317 5349 5391 5423 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2218 1240 223 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3022 4020 4266 669 Size: 988 281 1084 998 246 3079 Fragments arranged by size: 3079 1084 998 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6676 HpaI GTT'AAC Cuts at: 4266 4266 Size: 6676 KpnI G_GTAC'C Cuts at: 4001 4001 Size: 6676 MfeI C'AATT_G Cuts at: 4275 4275 Size: 6676 MluI A'CGCG_T Cuts at: 6552 6552 Size: 6676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5790 5974 1305 Size: 4485 184 2007 Fragments arranged by size: 4485 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3770 10 Size: 54 1425 343 1938 2916 Fragments arranged by size: 2916 1938 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2957 4634 4993 5152 518 Size: 648 843 948 1677 359 159 2042 Fragments arranged by size: 2042 1677 948 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4092 4769 5917 6162 1606 Size: 2486 677 1148 245 2120 Fragments arranged by size: 2486 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2283 513 Size: 552 939 279 4906 Fragments arranged by size: 4906 939 552 279 NdeI CA'TA_TG Cuts at: 387 387 Size: 6676 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6676 NotI GC'GGCC_GC Cuts at: 4123 4123 Size: 6676 NsiI A_TGCA'T Cuts at: 3375 3375 Size: 6676 NspI r_CATG'y Cuts at: 1132 1229 3671 4034 5579 1132 Size: 97 2442 363 1545 2229 Fragments arranged by size: 2442 2229 1545 363 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6676 PciI A'CATG_T Cuts at: 5575 5575 Size: 6676 PflMI CCAn_nnn'nTGG Cuts at: 1108 3616 1108 Size: 2508 4168 PmeI GTTT'AAAC Cuts at: 3980 3980 Size: 6676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4745 4777 6258 6290 4745 Size: 32 1481 32 5131 Fragments arranged by size: 5131 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6676 PsiI TTA'TAA Cuts at: 4246 4246 Size: 6676 PspXI vC'TCGA_Gb Cuts at: 3651 3651 Size: 6676 PstI C_TGCA'G Cuts at: 830 3412 4028 830 Size: 2582 616 3478 Fragments arranged by size: 3478 2582 616 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6676 SacI G_AGCT'C Cuts at: 2634 2634 Size: 6676 SalI G'TCGA_C Cuts at: 1936 4018 1936 Size: 2082 4594 SbfI CC_TGCA'GG Cuts at: 4028 4028 Size: 6676 ScaI AGT'ACT Cuts at: 2626 4924 6636 2626 Size: 2298 1712 2666 Fragments arranged by size: 2666 2298 1712 SfcI C'TryA_G Cuts at: 826 1045 3055 3408 4024 5159 5840 6031 826 Size: 219 2010 353 616 1135 681 191 1471 Fragments arranged by size: 2010 1471 1135 681 616 353 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6676 SmaI CCC'GGG Cuts at: 1872 4003 1872 Size: 2131 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3651 4780 5681 5943 820 Size: 197 926 1708 1129 901 262 1553 Fragments arranged by size: 1708 1553 1129 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4467 493 Size: 3974 2702 SpeI A'CTAG_T Cuts at: 152 2522 152 Size: 2370 4306 SphI G_CATG'C Cuts at: 1132 3671 4034 1132 Size: 2539 363 3774 Fragments arranged by size: 3774 2539 363 SspI AAT'ATT Cuts at: 5 52 2181 4600 5 Size: 47 2129 2419 2081 Fragments arranged by size: 2419 2129 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6676 StyI C'CwwG_G Cuts at: 513 1065 2004 2283 4115 513 Size: 552 939 279 1832 3074 Fragments arranged by size: 3074 1832 939 552 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2514 4703 4720 4873 5058 5503 325 Size: 1255 934 2189 17 153 185 445 1498 Fragments arranged by size: 2189 1498 1255 934 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2240 2624 4922 Size: 274 80 33 51 1443 263 384 2298 Cuts at: 4922 6634 96 Size: 1712 138 Fragments arranged by size: 2298 1712 1443 384 274 263 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2377 2409 3262 3294 6636 6668 2377 Size: 32 853 32 3342 32 2385 Fragments arranged by size: 3342 2385 853 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2543 1316 Size: 1227 5449 XbaI T'CTAG_A Cuts at: 2194 4012 2194 Size: 1818 4858 XhoI C'TCGA_G Cuts at: 1943 3651 1943 Size: 1708 4968 XmaI C'CCGG_G Cuts at: 1870 4001 1870 Size: 2131 4545 XmnI GAAnn'nnTTC Cuts at: 3050 4805 3050 Size: 1755 4921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4484 276 Size: 53 83 186 936 2950 2468 Fragments arranged by size: 2950 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI