(Circular) (Six-base) MAPSORT of: pFN21AB8669.seq Check: 8225 from: 1 to: 6475 pFN21AB8669 6475 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2764 2764 Size: 6475 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3818 1937 Size: 1881 4594 Acc65I G'GTAC_C Cuts at: 2679 2928 3796 2679 Size: 249 868 5358 Fragments arranged by size: 5358 868 249 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3139 3470 4538 5922 6190 1632 Size: 1507 331 1068 1384 268 1917 Fragments arranged by size: 1917 1507 1384 1068 331 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 5374 6351 1203 Size: 4171 977 1327 Fragments arranged by size: 4171 1327 977 AgeI A'CCGG_T Cuts at: 5306 5306 Size: 6475 AhdI GACnn_n'nnGTC Cuts at: 5204 5204 Size: 6475 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6475 AlwNI CAG_nnn'CTG Cuts at: 2513 2591 2716 2876 2974 4218 5790 2513 Size: 78 125 160 98 1244 1572 3198 Fragments arranged by size: 3198 1572 1244 160 125 98 78 ApaI G_GGCC'C Cuts at: 2870 3620 2870 Size: 750 5725 ApaLI G'TGCA_C Cuts at: 3068 4532 5688 3068 Size: 1464 1156 3855 Fragments arranged by size: 3855 1464 1156 ApoI r'AATT_y Cuts at: 1493 3784 4010 4162 6271 1493 Size: 2291 226 152 2109 1697 Fragments arranged by size: 2291 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 2136 3527 3800 1870 Size: 73 193 1391 273 4545 Fragments arranged by size: 4545 1391 273 193 73 AvrII C'CTAG_G Cuts at: 2302 2302 Size: 6475 BamHI G'GATC_C Cuts at: 2961 3805 4172 2961 Size: 844 367 5264 Fragments arranged by size: 5264 844 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2100 2245 2346 2665 2679 Size: 325 217 627 313 145 101 319 14 Cuts at: 2679 2896 2928 3118 3342 3796 5251 618 Size: 217 32 190 224 454 1455 1842 Fragments arranged by size: 1842 1455 627 454 325 319 313 224 217 217 190 145 101 32 14 BanII G_rGCy'C Cuts at: 1391 2481 2870 3309 3552 3620 3794 1391 Size: 1090 389 439 243 68 174 4072 Fragments arranged by size: 4072 1090 439 389 243 174 68 BbeI G_GCGC'C Cuts at: 3346 3346 Size: 6475 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3083 3085 928 Size: 971 1184 2 4318 Fragments arranged by size: 4318 1184 971 2 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4666 4700 1903 Size: 34 2729 34 3678 Fragments arranged by size: 3678 2729 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2514 4367 5577 2514 Size: 1853 1210 3412 Fragments arranged by size: 3412 1853 1210 BclI T'GATC_A Cuts at: 1548 3429 1548 Size: 1881 4594 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2334 2370 2847 Size: 107 122 71 631 605 662 36 477 Cuts at: 2847 3134 3615 5086 136 Size: 287 481 1471 1525 Fragments arranged by size: 1525 1471 662 631 605 481 477 287 122 107 71 36 BglII A'GATC_T Cuts at: 3024 3295 6470 3024 Size: 271 3175 3029 Fragments arranged by size: 3175 3029 271 BlpI GC'TnA_GC Cuts at: 2433 3551 3892 2433 Size: 1118 341 5016 Fragments arranged by size: 5016 1118 341 Bme1580I G_kGCm'C Cuts at: 1790 2044 2250 2349 2638 2870 2899 3072 3123 Size: 254 206 99 289 232 29 173 51 Cuts at: 3123 3588 3620 4536 5692 1790 Size: 465 32 916 1156 2573 Fragments arranged by size: 2573 1156 916 465 289 254 232 206 173 99 51 32 29 BmgBI CAC'GTC Cuts at: 1318 3520 1318 Size: 2202 4273 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2607 2625 2632 Size: 416 53 83 399 24 1190 18 7 Cuts at: 2632 3661 4206 5164 442 Size: 1029 545 958 1753 Fragments arranged by size: 1753 1190 1029 958 545 416 399 83 53 24 18 7 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2587 3298 2587 Size: 711 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 2799 2898 3245 3280 5135 1239 Size: 1223 337 99 347 35 1855 2579 Fragments arranged by size: 2579 1855 1223 347 337 99 35 Bpu10I CC'TnA_GC Cuts at: 2475 2562 2475 Size: 87 6388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4600 5465 5763 4600 Size: 865 298 5312 Fragments arranged by size: 5312 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2059 5138 882 Size: 617 560 3079 2219 Fragments arranged by size: 3079 2219 617 560 BsaAI yAC'GTr Cuts at: 493 1204 3415 4266 493 Size: 711 2211 851 2702 Fragments arranged by size: 2702 2211 851 711 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3343 3445 4282 4664 Size: 53 83 186 936 1810 102 837 382 Cuts at: 4664 5299 275 Size: 635 1451 Fragments arranged by size: 1810 1451 936 837 635 382 186 102 83 53 BsaWI w'CCGG_w Cuts at: 4908 5306 5580 5727 6138 4908 Size: 398 274 147 411 5245 Fragments arranged by size: 5245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6250 Fragments arranged by size: 6250 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2759 1186 Size: 1573 4902 BseYI C'CCAG_C Cuts at: 2241 2383 2534 2683 2706 2724 2735 5678 6174 Size: 142 151 149 23 18 11 2943 496 Cuts at: 6174 2241 Size: 2542 Fragments arranged by size: 2943 2542 496 151 149 142 23 18 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3134 1163 Size: 573 1398 4504 Fragments arranged by size: 4504 1398 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3339 3643 3925 4686 5714 1540 Size: 275 186 1338 304 282 761 1028 2301 Fragments arranged by size: 2301 1338 1028 761 304 282 275 186 BsiHKAI G_wGCw'C Cuts at: 3072 3309 3651 3693 4536 4621 5692 3072 Size: 237 342 42 843 85 1071 3855 Fragments arranged by size: 3855 1071 843 342 237 85 42 BsmI GAATG_Cn' Cuts at: 3986 4079 3986 Size: 93 6382 BsmBI CGTCTCn'nnnn_ Cuts at: 3095 3095 Size: 6475 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2250 2349 2481 2638 2870 2899 Size: 399 254 206 99 132 157 232 29 Cuts at: 2899 3072 3123 3309 3552 3588 3620 3651 3693 Size: 173 51 186 243 36 32 31 42 Cuts at: 3693 3794 4536 4621 5692 1391 Size: 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 254 243 232 206 186 173 157 132 101 99 85 51 42 36 32 31 29 BspEI T'CCGG_A Cuts at: 6138 6138 Size: 6475 BspHI T'CATG_A Cuts at: 4364 6094 4364 Size: 1730 4745 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2641 2764 3039 3830 844 Size: 1797 123 275 791 3489 Fragments arranged by size: 3489 1797 791 275 123 BsrBI CCG'CTC Cuts at: 1394 1823 2859 3256 4362 1394 Size: 429 1036 397 1106 3507 Fragments arranged by size: 3507 1106 1036 429 397 BsrDI GCAATG_nn' Cuts at: 65 1248 4970 5144 65 Size: 1183 3722 174 1396 Fragments arranged by size: 3722 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2034 2850 5119 5306 1700 Size: 110 224 816 2269 187 2869 Fragments arranged by size: 2869 2269 816 224 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6475 BssSI C'ACGA_G Cuts at: 4535 5547 4535 Size: 1012 5463 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6475 BstBI TT'CG_AA Cuts at: 5279 5279 Size: 6475 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6475 BstXI CCAn_nnnn'nTGG Cuts at: 2837 2837 Size: 6475 BstYI r'GATC_y Cuts at: 1972 2961 3024 3295 3805 4172 4557 4574 6026 Size: 989 63 271 510 367 385 17 1452 Cuts at: 6026 6112 6124 6470 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 989 510 385 367 346 271 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 3333 3561 3684 513 Size: 552 2268 228 123 3304 Fragments arranged by size: 3304 2268 552 228 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3398 3541 487 Size: 683 56 2172 143 3421 Fragments arranged by size: 3421 2172 683 143 56 BtsI GCAGTG_nn' Cuts at: 2280 2532 2746 3991 4785 4805 2280 Size: 252 214 1245 794 20 3950 Fragments arranged by size: 3950 1245 794 252 214 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DraIII CAC_nnn'GTG Cuts at: 2903 2903 Size: 6475 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3017 5482 809 Size: 507 1701 2465 1802 Fragments arranged by size: 2465 1802 1701 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2371 2529 2848 Size: 54 1425 50 275 18 541 158 319 Cuts at: 2848 2905 3126 3199 3231 3336 3640 3666 3922 Size: 57 221 73 32 105 304 26 256 Cuts at: 3922 4811 6440 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 541 319 304 275 256 221 158 105 73 57 54 50 43 32 26 18 EagI C'GGCC_G Cuts at: 1537 1812 3336 3640 3922 1537 Size: 275 1524 304 282 4090 Fragments arranged by size: 4090 1524 304 282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2225 4405 1353 Size: 872 2180 3423 Fragments arranged by size: 3423 2180 872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2352 2869 5062 5436 5582 387 Size: 1064 138 763 517 2193 374 146 1280 Fragments arranged by size: 2193 1280 1064 763 517 374 146 138 EcoICRI GAG'CTC Cuts at: 3307 3307 Size: 6475 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2462 2799 2898 3139 3245 3280 3470 Size: 393 830 337 99 241 106 35 190 Cuts at: 3470 4538 5135 5922 6190 1239 Size: 1068 597 787 268 1524 Fragments arranged by size: 1524 1068 830 787 597 393 337 268 241 190 106 99 35 EcoO109I rG'GnC_Cy Cuts at: 2306 2866 2971 3617 2306 Size: 560 105 646 5164 Fragments arranged by size: 5164 646 560 105 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3314 3314 Size: 6475 FspI TGC'GCA Cuts at: 2294 4981 2294 Size: 2687 3788 HaeII r_GCGC'y Cuts at: 1678 2752 3346 3509 5622 1678 Size: 1074 594 163 2113 2531 Fragments arranged by size: 2531 2113 1074 594 163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 3164 3196 5116 Size: 32 135 32 911 32 649 32 1920 Cuts at: 5116 5148 5190 5222 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1920 911 649 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3819 4065 669 Size: 988 281 1881 246 3079 Fragments arranged by size: 3079 1881 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6475 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KasI G'GCGC_C Cuts at: 3342 3342 Size: 6475 KpnI G_GTAC'C Cuts at: 2683 2932 3800 2683 Size: 249 868 5358 Fragments arranged by size: 5358 868 249 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3021 5589 5773 1305 Size: 1716 2568 184 2007 Fragments arranged by size: 2568 2007 1716 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2373 3668 10 Size: 54 1425 343 541 1295 2817 Fragments arranged by size: 2817 1425 1295 541 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 2400 2406 3560 4433 4792 4951 Size: 648 993 241 6 1154 873 359 159 Cuts at: 4951 518 Size: 2042 Fragments arranged by size: 2042 1154 993 873 648 359 241 159 6 MspA1I CmG'CkG Cuts at: 1606 2534 2950 3891 4568 5716 5961 1606 Size: 928 416 941 677 1148 245 2120 Fragments arranged by size: 2120 1148 941 928 677 416 245 NaeI GCC'GGC Cuts at: 2036 2852 2036 Size: 816 5659 NarI GG'CG_CC Cuts at: 3343 3343 Size: 6475 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5923 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NgoMIV G'CCGG_C Cuts at: 2034 2850 2034 Size: 816 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 3833 5378 1132 Size: 97 2604 1545 2229 Fragments arranged by size: 2604 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6475 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 2876 2953 1108 Size: 1768 77 4630 Fragments arranged by size: 4630 1768 77 PfoI T'CCnGG_A Cuts at: 2228 3222 2228 Size: 994 5481 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4544 4576 6057 6089 4544 Size: 32 1481 32 4930 Fragments arranged by size: 4930 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6475 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PspOMI G'GGCC_C Cuts at: 2866 3616 2866 Size: 750 5725 PstI C_TGCA'G Cuts at: 830 2518 2970 3827 830 Size: 1688 452 857 3478 Fragments arranged by size: 3478 1688 857 452 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 2950 2950 Size: 6475 SacI G_AGCT'C Cuts at: 3309 3309 Size: 6475 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SbfI CC_TGCA'GG Cuts at: 2970 3827 2970 Size: 857 5618 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SexAI A'CCwGG_T Cuts at: 2675 2675 Size: 6475 SfcI C'TryA_G Cuts at: 826 1045 2514 2966 3823 4958 5639 5830 826 Size: 219 1469 452 857 1135 681 191 1471 Fragments arranged by size: 1471 1469 1135 857 681 452 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2370 2847 3134 2370 Size: 477 287 5711 Fragments arranged by size: 5711 477 287 SfoI GGC'GCC Cuts at: 3344 3344 Size: 6475 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6475 SmaI CCC'GGG Cuts at: 1872 2138 3802 1872 Size: 266 1664 4545 Fragments arranged by size: 4545 1664 266 SmlI C'TyrA_G Cuts at: 820 1017 1943 4579 5480 5742 820 Size: 197 926 2636 901 262 1553 Fragments arranged by size: 2636 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4266 493 Size: 3773 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 3833 1132 Size: 2701 3774 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 2328 3422 1517 Size: 811 1094 4570 Fragments arranged by size: 4570 1094 811 StyI C'CwwG_G Cuts at: 513 1065 2302 2988 3063 3914 513 Size: 552 1237 686 75 851 3074 Fragments arranged by size: 3074 1237 851 686 552 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2133 2164 2198 2233 2357 2683 2720 Size: 1255 553 31 34 35 124 326 37 Cuts at: 2720 4502 4519 4672 4857 5302 325 Size: 1782 17 153 185 445 1498 Fragments arranged by size: 1782 1498 1255 553 445 326 185 153 124 37 35 34 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4721 6433 96 Size: 274 80 33 51 1443 2744 1712 138 Fragments arranged by size: 2744 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6435 6467 6435 Size: 32 6443 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6475 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2357 2357 Size: 6475 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6475 XmaI C'CCGG_G Cuts at: 1870 2136 3800 1870 Size: 266 1664 4545 Fragments arranged by size: 4545 1664 266 XmnI GAAnn'nnTTC Cuts at: 4604 4604 Size: 6475 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SrfI SwaI