(Circular) (Six-base) MAPSORT of: pFN21AB8661.seq Check: 9938 from: 1 to: 5965 pFN21AB8661 5965 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3775 278 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3308 1937 Size: 1371 4594 Acc65I G'GTAC_C Cuts at: 3286 3286 Size: 5965 AclI AA'CG_TT Cuts at: 1563 4092 4465 1563 Size: 2529 373 3063 Fragments arranged by size: 3063 2529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 4028 5412 5680 1632 Size: 597 1799 1384 268 1917 Fragments arranged by size: 1917 1799 1384 597 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5768 AflIII A'CryG_T Cuts at: 1203 2081 3150 4864 5841 1203 Size: 878 1069 1714 977 1327 Fragments arranged by size: 1714 1327 1069 977 878 AgeI A'CCGG_T Cuts at: 4796 4796 Size: 5965 AhdI GACnn_n'nnGTC Cuts at: 4694 4694 Size: 5965 AleI CACnn'nnGTG Cuts at: 1166 2792 1166 Size: 1626 4339 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5965 AlwNI CAG_nnn'CTG Cuts at: 2966 3708 5280 2966 Size: 742 1572 3651 Fragments arranged by size: 3651 1572 742 ApaLI G'TGCA_C Cuts at: 4022 5178 4022 Size: 1156 4809 ApoI r'AATT_y Cuts at: 1493 3274 3500 3652 5761 1493 Size: 1781 226 152 2109 1697 Fragments arranged by size: 2109 1781 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4519 160 Size: 872 3487 1606 Fragments arranged by size: 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 AvaI C'yCGr_G Cuts at: 1870 1943 3290 1870 Size: 73 1347 4545 Fragments arranged by size: 4545 1347 73 BamHI G'GATC_C Cuts at: 3295 3662 3295 Size: 367 5598 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2175 3286 4741 618 Size: 325 217 627 388 1111 1455 1842 Fragments arranged by size: 1842 1455 1111 627 388 325 217 BanII G_rGCy'C Cuts at: 1391 3007 3193 3241 3284 1391 Size: 1616 186 48 43 4072 Fragments arranged by size: 4072 1616 186 48 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2210 928 Size: 971 311 4683 Fragments arranged by size: 4683 971 311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4156 4190 1903 Size: 34 2219 34 3678 Fragments arranged by size: 3678 2219 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3857 5067 3857 Size: 1210 4755 BclI T'GATC_A Cuts at: 1548 2587 1548 Size: 1039 4926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4576 136 Size: 107 122 71 631 605 2904 1525 Fragments arranged by size: 2904 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5960 5960 Size: 5965 BlpI GC'TnA_GC Cuts at: 3382 3382 Size: 5965 Bme1580I G_kGCm'C Cuts at: 1790 2180 4026 5182 1790 Size: 390 1846 1156 2573 Fragments arranged by size: 2573 1846 1156 390 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5965 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2056 3696 4654 Size: 416 53 83 399 24 639 1640 958 Cuts at: 4654 442 Size: 1753 Fragments arranged by size: 1753 1640 958 639 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2202 3060 3177 4625 1239 Size: 963 858 117 1448 2579 Fragments arranged by size: 2579 1448 963 858 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4090 4955 5253 4090 Size: 865 298 4802 Fragments arranged by size: 4802 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4628 882 Size: 617 3129 2219 Fragments arranged by size: 3129 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3756 493 Size: 711 2552 2702 Fragments arranged by size: 2702 2552 711 BsaBI GATnn'nnATC Cuts at: 5959 5959 Size: 5965 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3772 4154 4789 275 Size: 53 83 186 936 2239 382 635 1451 Fragments arranged by size: 2239 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2778 4398 4796 5070 5217 5628 2778 Size: 1620 398 274 147 411 3115 Fragments arranged by size: 3115 1620 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2483 2513 1178 Size: 30 165 30 1080 30 4630 Fragments arranged by size: 4630 1080 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2544 3003 3006 1186 Size: 1358 459 3 4145 Fragments arranged by size: 4145 1358 459 3 BseYI C'CCAG_C Cuts at: 2413 5168 5664 2413 Size: 2755 496 2714 Fragments arranged by size: 2755 2714 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2816 1163 Size: 573 1080 4312 Fragments arranged by size: 4312 1080 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3415 4176 5204 1540 Size: 275 186 1414 761 1028 2301 Fragments arranged by size: 2301 1414 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3007 3241 4026 4111 5182 3007 Size: 234 785 85 1071 3790 Fragments arranged by size: 3790 1071 785 234 85 BsmI GAATG_Cn' Cuts at: 2141 3476 3569 2141 Size: 1335 93 4537 Fragments arranged by size: 4537 1335 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 3007 3193 3241 3284 4026 4111 Size: 399 390 827 186 48 43 742 85 Cuts at: 4111 5182 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 827 742 399 390 186 85 48 43 BspEI T'CCGG_A Cuts at: 2778 5628 2778 Size: 2850 3115 BspHI T'CATG_A Cuts at: 3854 5584 3854 Size: 1730 4235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2231 2261 3320 844 Size: 1387 30 1059 3489 Fragments arranged by size: 3489 1387 1059 30 BsrBI CCG'CTC Cuts at: 1394 1823 3852 1394 Size: 429 2029 3507 Fragments arranged by size: 3507 2029 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3090 3114 4460 4634 65 Size: 1183 1842 24 1346 174 1396 Fragments arranged by size: 1842 1396 1346 1183 174 24 BsrFI r'CCGG_y Cuts at: 1700 1810 4609 4796 1700 Size: 110 2799 187 2869 Fragments arranged by size: 2869 2799 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5965 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5965 BssSI C'ACGA_G Cuts at: 4025 5037 4025 Size: 1012 4953 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5965 BstBI TT'CG_AA Cuts at: 4769 4769 Size: 5965 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5965 BstYI r'GATC_y Cuts at: 1972 2566 2775 2915 3295 3662 4047 4064 5516 Size: 594 209 140 380 367 385 17 1452 Cuts at: 5516 5602 5614 5960 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 594 385 380 367 346 209 140 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4474 Fragments arranged by size: 4474 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5226 Fragments arranged by size: 5226 683 56 BtsI GCAGTG_nn' Cuts at: 2813 3481 4275 4295 2813 Size: 668 794 20 4483 Fragments arranged by size: 4483 794 668 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5930 DraI TTT'AAA Cuts at: 2314 3269 2314 Size: 955 5010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4972 809 Size: 507 3656 1802 Fragments arranged by size: 3656 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2399 2887 3412 Size: 54 1425 50 275 18 569 488 525 Cuts at: 3412 4301 5930 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 569 525 488 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3412 1537 Size: 275 1600 4090 Fragments arranged by size: 4090 1600 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3012 3895 1353 Size: 1659 883 3423 Fragments arranged by size: 3423 1659 883 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4552 4926 5072 387 Size: 1064 138 2963 374 146 1280 Fragments arranged by size: 2963 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3005 3239 3005 Size: 234 5731 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2202 2229 3060 3177 4028 4625 5412 Size: 393 570 27 831 117 851 597 787 Cuts at: 5412 5680 1239 Size: 268 1524 Fragments arranged by size: 1524 851 831 787 597 570 393 268 117 27 EcoNI CCTnn'n_nnAGG Cuts at: 2035 2988 2035 Size: 953 5012 EcoO109I rG'GnC_Cy Cuts at: 3220 3220 Size: 5965 EcoRI G'AATT_C Cuts at: 3274 3652 3274 Size: 378 5587 FspI TGC'GCA Cuts at: 4471 4471 Size: 5965 HaeII r_GCGC'y Cuts at: 1678 5112 1678 Size: 3434 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3223 3255 4606 4638 4680 Size: 32 135 32 1651 32 1351 32 42 Cuts at: 4680 4712 1373 Size: 32 2626 Fragments arranged by size: 2626 1651 1351 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2341 3309 3555 669 Size: 988 281 403 968 246 3079 Fragments arranged by size: 3079 988 968 403 281 246 HindIII A'AGCT_T Cuts at: 748 2639 748 Size: 1891 4074 HpaI GTT'AAC Cuts at: 3555 3555 Size: 5965 KpnI G_GTAC'C Cuts at: 3290 3290 Size: 5965 MfeI C'AATT_G Cuts at: 3564 3564 Size: 5965 MluI A'CGCG_T Cuts at: 5841 5841 Size: 5965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 5079 5263 1305 Size: 744 3030 184 2007 Fragments arranged by size: 3030 2007 744 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4143 Fragments arranged by size: 4143 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2792 3923 4282 4441 518 Size: 648 1626 1131 359 159 2042 Fragments arranged by size: 2042 1626 1131 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3080 3381 4058 5206 5451 1606 Size: 1474 301 677 1148 245 2120 Fragments arranged by size: 2120 1474 1148 677 301 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4474 Fragments arranged by size: 4474 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5965 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5965 NotI GC'GGCC_GC Cuts at: 3412 3412 Size: 5965 NspI r_CATG'y Cuts at: 1132 1229 2085 2194 2326 2413 2443 3154 3323 Size: 97 856 109 132 87 30 711 169 Cuts at: 3323 4868 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 856 711 169 132 109 97 87 30 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5965 PciI A'CATG_T Cuts at: 2081 3150 4864 2081 Size: 1069 1714 3182 Fragments arranged by size: 3182 1714 1069 PflMI CCAn_nnn'nTGG Cuts at: 1108 3140 3163 1108 Size: 2032 23 3910 Fragments arranged by size: 3910 2032 23 PfoI T'CCnGG_A Cuts at: 2989 3193 2989 Size: 204 5761 PmeI GTTT'AAAC Cuts at: 3269 3269 Size: 5965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4034 4066 5547 5579 4034 Size: 32 1481 32 4420 Fragments arranged by size: 4420 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3220 3220 Size: 5965 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5965 PsiI TTA'TAA Cuts at: 3535 3535 Size: 5965 PstI C_TGCA'G Cuts at: 830 2228 2845 3025 3317 830 Size: 1398 617 180 292 3478 Fragments arranged by size: 3478 1398 617 292 180 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5965 PvuII CAG'CTG Cuts at: 3080 3080 Size: 5965 SacI G_AGCT'C Cuts at: 3007 3241 3007 Size: 234 5731 SalI G'TCGA_C Cuts at: 1936 3307 1936 Size: 1371 4594 SapI GCTCTTCn'nnn_ Cuts at: 3012 3012 Size: 5965 SbfI CC_TGCA'GG Cuts at: 3317 3317 Size: 5965 ScaI AGT'ACT Cuts at: 2351 4213 5925 2351 Size: 1862 1712 2391 Fragments arranged by size: 2391 1862 1712 SfcI C'TryA_G Cuts at: 826 1045 2224 2562 2628 2841 2958 3021 3313 Size: 219 1179 338 66 213 117 63 292 Cuts at: 3313 4448 5129 5320 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1179 1135 681 338 292 219 213 191 117 66 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5965 SmaI CCC'GGG Cuts at: 1872 3292 1872 Size: 1420 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4069 4970 5232 820 Size: 197 926 2126 901 262 1553 Fragments arranged by size: 2126 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3756 493 Size: 3263 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5965 SphI G_CATG'C Cuts at: 1132 2194 2413 3323 1132 Size: 1062 219 910 3774 Fragments arranged by size: 3774 1062 910 219 SspI AAT'ATT Cuts at: 5 52 2596 3889 5 Size: 47 2544 1293 2081 Fragments arranged by size: 2544 2081 1293 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5965 StyI C'CwwG_G Cuts at: 513 1065 2004 3404 513 Size: 552 939 1400 3074 Fragments arranged by size: 3074 1400 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2497 3174 3992 4009 4162 4347 4792 Size: 1255 917 677 818 17 153 185 445 Cuts at: 4792 325 Size: 1498 Fragments arranged by size: 1498 1255 917 818 677 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2349 4211 5923 Size: 274 80 33 51 1443 372 1862 1712 Cuts at: 5923 96 Size: 138 Fragments arranged by size: 1862 1712 1443 372 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2483 2515 3138 3170 5925 5957 2483 Size: 32 623 32 2755 32 2491 Fragments arranged by size: 2755 2491 623 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5965 XbaI T'CTAG_A Cuts at: 3301 3301 Size: 5965 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2084 2084 Size: 5965 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5965 XmaI C'CCGG_G Cuts at: 1870 3290 1870 Size: 1420 4545 XmnI GAAnn'nnTTC Cuts at: 2985 4094 2985 Size: 1109 4856 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3773 276 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI