Restriction map for
pFN21AB8656
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstNI BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRII EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HpaI KasI
KpnI KspI MluI MluNI MroI
MscI MvaI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 289 CCGC CCGC(-3/-1)
AflIII 305 ACATGT A^C[GA][CT]GT
AgeI 536 ACCGGT A^CCGGT
AlwI 573 GGATC GGATC(4/5)
AocI 278 CCTCAGG CC^T[ACGT]AGG
ApaI 469 GGGCCC GGGCC^C
BanI 1332 GGCACC G^G[CT][GA]CC
BfaI 352 CTAG C^TAG
BsaWI 536 ACCGGT [AT]^CCGG[AT]
BseRI 626 CTCCTC GAGGAG(10/8)
BsgI 1164 CTGCAC GTGCAG(16/14)
BsmFI 766 GGGAC GGGAC(10/14)
BspHI 1635 TCATGA T^CATGA
BspLU11I 305 ACATGT A^CATGT
BsrFI 536 ACCGGT [GA]^CCGG[CT]
BstBI 555 TTCGAA TT^CGAA
BstYI 573 GGATCT [GA]^GATC[CT]
Bsu36I 278 CCTCAGG CC^T[ACGT]AGG
CfoI 1128 GCGC GCG^C
Cfr10I 536 ACCGGT [GA]^CCGG[CT]
DraII 469 GGGCCCC [GA]G^G[ACGT]CC[CT]
Eco81I 278 CCTCAGG CC^T[ACGT]AGG
EcoO109I 469 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1239 GAATTC G^AATTC
EcoT14I 928 CCAAGG C^C[AT][AT]GG
HhaI 1128 GCGC GCG^C
HinP1I 1128 GCGC G^CGC
HincII 1295 GTCAAC GT[CT]^[GA]AC
HindII 1295 GTCAAC GT[CT]^[GA]AC
HindIII 747 AAGCTT A^AGCTT
MaeI 352 CTAG C^TAG
MaeII 1293 ACGT A^CGT
MfeI 613 CAATTG C^AATTG
MflI 573 GGATCT [GA]^GATC[CT]
MslI 1665 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 770 CAGCTG C[AC]G^C[GT]G
MunI 613 CAATTG C^AATTG
NdeI 833 CATATG CA^TATG
NspV 555 TTCGAA TT^CGAA
PinAI 536 ACCGGT A^CCGGT
PstI 95 CTGCAG CTGCA^G
PvuII 770 CAGCTG CAG^CTG
RcaI 1635 TCATGA T^CATGA
SacI 483 GAGCTC GAGCT^C
SapI 910 GCTCTTC GCTCTTC(1/4)
SfcI 95 CTGCAG C^T[GA][CT]AG
SfuI 555 TTCGAA TT^CGAA
SmlI 75 CTTGAG C^T[CT][GA]AG
SpeI 351 ACTAGT A^CTAGT
StyI 928 CCAAGG C^C[AT][AT]GG
TfiI 793 GATTC G^A[AT]TC
XhoII 573 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 128 GTAC GT^AC
355 GTAC
AspHI 78 GAGCAC G[AT]GC[AT]^C
483 GAGCTC
AvaII 49 GGTCC G^G[AT]CC
415 GGTCC
BcnI 467 CCGGG CC^[GC]GG
473 CCCGG
BsaBI 329 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1465 GATGAAAATC
BsaJI 472 CCCCGG C^C[ACGT][ACGT]GG
928 CCAAGG
BsiHKAI 78 GAGCAC G[AT]GC[AT]^C
483 GAGCTC
BsmAI 369 GAGAC GTCTC(1/5)
775 GAGAC
BsmI 918 GCATTC GAATGC(1/-1)
1401 GCATTC
BspMI 684 ACCTGC ACCTGC(4/8)
1133 ACCTGC
BsrDI 220 GCAATG GCAATG(2/0)
934 GCAATG
BssKI 467 CCGGG ^CC[ACGT]GG
473 CCCGG
Cac8I 225 GCGAGC GC[ACGT]^[ACGT]GC
813 GCAGGC
DraIII 185 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
563 CACCCAGTG
HaeIII 470 GGCC GG^CC
509 GGCC
MamI 329 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1465 GATGAAAATC
MwoI 220 GCAATGCGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1128 GCGCCACCTGC
NciI 467 CCGGG CC^[GC]GG
473 CCCGG
NspI 305 ACATGT [GA]CATG^[CT]
1140 GCATGT
PleI 880 GACTC GAGTC(4/5)
17 GAGTC
RsaI 128 GTAC GT^AC
355 GTAC
ScrFI 467 CCGGG CC^[ACGT]GG
473 CCCGG
TaqI 556 TCGA T^CGA
1082 TCGA
TthHB8I 556 TCGA T^CGA
1082 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccI AcsI AluI ApoI BanII
BbvI BmyI BpmI BsiYI BslI
Bsp1286I BsrI BstF5I Cfr13I DdeI
DpnII DraI EarI EcoNI Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MspI NdeII NlaIII
NlaIV Sau3AI Sau96I SfaNI Tru9I
TseI Tsp45I Tsp509I TspRI