(Circular) (Six-base) MAPSORT of: pFN21AB8656.seq Check: 5184 from: 1 to: 6406 pFN21AB8656 6406 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2698 3147 2698 Size: 449 5957 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2106 2389 3159 3749 1937 Size: 169 283 770 590 4594 Fragments arranged by size: 4594 770 590 283 169 Acc65I G'GTAC_C Cuts at: 3727 3727 Size: 6406 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2352 2903 2978 4469 5853 6121 1632 Size: 720 551 75 1491 1384 268 1917 Fragments arranged by size: 1917 1491 1384 720 551 268 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 2310 5305 6282 1203 Size: 1107 2995 977 1327 Fragments arranged by size: 2995 1327 1107 977 AgeI A'CCGG_T Cuts at: 2541 5237 2541 Size: 2696 3710 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6406 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2125 1184 Size: 941 5465 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2423 2455 2423 Size: 32 6374 AlwNI CAG_nnn'CTG Cuts at: 4149 5721 4149 Size: 1572 4834 ApaI G_GGCC'C Cuts at: 2478 2478 Size: 6406 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 2533 3039 3244 3377 3715 3941 4093 6202 Size: 1040 506 205 133 338 226 152 2109 Cuts at: 6202 1493 Size: 1697 Fragments arranged by size: 2109 1697 1040 506 338 226 205 152 133 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 3731 1870 Size: 73 1788 4545 Fragments arranged by size: 4545 1788 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2990 3023 2990 Size: 33 6373 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3337 3727 5182 618 Size: 325 217 627 1550 390 1455 1842 Fragments arranged by size: 1842 1550 1455 627 390 325 217 BanII G_rGCy'C Cuts at: 1391 2283 2478 2492 2500 3725 1391 Size: 892 195 14 8 1225 4072 Fragments arranged by size: 4072 1225 892 195 14 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5435 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4597 4631 1903 Size: 34 2660 34 3678 Fragments arranged by size: 3678 2660 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5017 136 Size: 107 122 71 631 605 3345 1525 Fragments arranged by size: 3345 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 2478 4467 5623 1790 Size: 688 1989 1156 2573 Fragments arranged by size: 2573 1989 1156 688 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2565 4137 5095 Size: 416 53 83 399 24 1148 1572 958 Cuts at: 5095 442 Size: 1753 Fragments arranged by size: 1753 1572 1148 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2189 2449 3202 2189 Size: 260 753 5393 Fragments arranged by size: 5393 753 260 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2419 2514 3601 5066 1239 Size: 1180 95 1087 1465 2579 Fragments arranged by size: 2579 1465 1180 1087 95 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2101 4531 5396 5694 2101 Size: 2430 865 298 2813 Fragments arranged by size: 2813 2430 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5069 882 Size: 617 3570 2219 Fragments arranged by size: 3570 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4197 493 Size: 711 2993 2702 Fragments arranged by size: 2993 2702 711 BsaBI GATnn'nnATC Cuts at: 2338 3474 6400 2338 Size: 1136 2926 2344 Fragments arranged by size: 2926 2344 1136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4213 4595 5230 275 Size: 53 83 186 936 2680 382 635 1451 Fragments arranged by size: 2680 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2541 4839 5237 5511 5658 6069 2541 Size: 2298 398 274 147 411 2878 Fragments arranged by size: 2878 2298 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6181 Fragments arranged by size: 6181 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2622 1186 Size: 1436 4970 BseYI C'CCAG_C Cuts at: 3239 5609 6105 3239 Size: 2370 496 3540 Fragments arranged by size: 3540 2370 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3154 1163 Size: 573 1418 4415 Fragments arranged by size: 4415 1418 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3856 4617 5645 1540 Size: 275 186 1855 761 1028 2301 Fragments arranged by size: 2301 1855 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2087 2492 4467 4552 5623 2087 Size: 405 1975 85 1071 2870 Fragments arranged by size: 2870 1975 1071 405 85 BsmI GAATG_Cn' Cuts at: 2923 3406 3917 4010 2923 Size: 483 511 93 5319 Fragments arranged by size: 5319 511 483 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2283 2478 2492 2500 3725 4467 Size: 399 297 196 195 14 8 1225 742 Cuts at: 4467 4552 5623 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1225 1071 742 399 297 196 195 85 14 8 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 3640 4295 6025 3640 Size: 655 1730 4021 Fragments arranged by size: 4021 1730 655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2698 3147 3761 844 Size: 1854 449 614 3489 Fragments arranged by size: 3489 1854 614 449 BsrBI CCG'CTC Cuts at: 1394 1823 4293 1394 Size: 429 2470 3507 Fragments arranged by size: 3507 2470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2232 2946 4901 5075 65 Size: 1183 984 714 1955 174 1396 Fragments arranged by size: 1955 1396 1183 984 714 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2541 5050 5237 1700 Size: 110 731 2509 187 2869 Fragments arranged by size: 2869 2509 731 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6406 BstBI TT'CG_AA Cuts at: 2561 5210 2561 Size: 2649 3757 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6406 BstYI r'GATC_y Cuts at: 1972 2578 3736 4103 4488 4505 5957 6043 6055 Size: 606 1158 367 385 17 1452 86 12 Cuts at: 6055 6401 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1158 606 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2284 2284 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4915 Fragments arranged by size: 4915 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5667 Fragments arranged by size: 5667 683 56 BtsI GCAGTG_nn' Cuts at: 3922 4716 4736 3922 Size: 794 20 5592 Fragments arranged by size: 5592 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 2071 2643 2758 3710 2071 Size: 572 115 952 4767 Fragments arranged by size: 4767 952 572 115 DraIII CAC_nnn'GTG Cuts at: 2195 2573 2195 Size: 378 6028 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3853 4742 6371 Size: 54 1425 50 275 18 2023 889 1629 Cuts at: 6371 8 Size: 43 Fragments arranged by size: 2023 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3853 1537 Size: 275 2041 4090 Fragments arranged by size: 4090 2041 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2158 2371 2922 4336 1353 Size: 805 213 551 1414 3423 Fragments arranged by size: 3423 1414 805 551 213 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2490 2490 Size: 6406 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2352 2419 2514 2903 2978 3601 4469 Size: 393 720 67 95 389 75 623 868 Cuts at: 4469 5066 5853 6121 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 868 787 720 623 597 393 389 268 95 75 67 EcoNI CCTnn'n_nnAGG Cuts at: 2347 2874 2932 2347 Size: 527 58 5821 Fragments arranged by size: 5821 527 58 EcoO109I rG'GnC_Cy Cuts at: 2475 2475 Size: 6406 EcoRI G'AATT_C Cuts at: 3244 3715 4093 3244 Size: 471 378 5557 Fragments arranged by size: 5557 471 378 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 5553 1678 Size: 3875 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5047 5079 5121 5153 1373 Size: 32 135 32 3475 32 42 32 2626 Fragments arranged by size: 3475 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3302 3750 3996 669 Size: 988 281 1364 448 246 3079 Fragments arranged by size: 3079 1364 988 448 281 246 HindIII A'AGCT_T Cuts at: 748 2752 748 Size: 2004 4402 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KpnI G_GTAC'C Cuts at: 3731 3731 Size: 6406 MfeI C'AATT_G Cuts at: 2618 4005 2618 Size: 1387 5019 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5520 5704 1305 Size: 4215 184 2007 Fragments arranged by size: 4215 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4584 Fragments arranged by size: 4584 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3674 4364 4723 4882 518 Size: 648 2508 690 359 159 2042 Fragments arranged by size: 2508 2042 690 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2777 3822 4499 5647 5892 1606 Size: 1171 1045 677 1148 245 2120 Fragments arranged by size: 2120 1171 1148 1045 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4915 Fragments arranged by size: 4915 939 552 NdeI CA'TA_TG Cuts at: 387 2839 387 Size: 2452 3954 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 2314 3149 3764 5309 1132 Size: 97 1085 835 615 1545 2229 Fragments arranged by size: 2229 1545 1085 835 615 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 2310 5305 2310 Size: 2995 3411 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6406 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4475 4507 5988 6020 4475 Size: 32 1481 32 4861 Fragments arranged by size: 4861 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 2241 3396 3422 3976 2241 Size: 1155 26 554 4671 Fragments arranged by size: 4671 1155 554 26 PspOMI G'GGCC_C Cuts at: 2474 2474 Size: 6406 PstI C_TGCA'G Cuts at: 830 2104 3758 830 Size: 1274 1654 3478 Fragments arranged by size: 3478 1654 1274 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2777 2777 Size: 6406 SacI G_AGCT'C Cuts at: 2492 2492 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SapI GCTCTTCn'nnn_ Cuts at: 2922 2922 Size: 6406 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SfcI C'TryA_G Cuts at: 826 1045 2100 3754 4889 5570 5761 826 Size: 219 1055 1654 1135 681 191 1471 Fragments arranged by size: 1654 1471 1135 1055 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2080 4510 5411 5673 820 Size: 197 926 137 2430 901 262 1553 Fragments arranged by size: 2430 1553 926 901 262 197 137 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 2356 152 Size: 2204 4202 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6406 StyI C'CwwG_G Cuts at: 513 1065 2004 2933 3845 513 Size: 552 939 929 912 3074 Fragments arranged by size: 3074 939 929 912 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2584 4433 4450 4603 4788 5233 325 Size: 1255 1004 1849 17 153 185 445 1498 Fragments arranged by size: 1849 1498 1255 1004 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4652 6364 96 Size: 274 80 33 51 1443 2675 1712 138 Fragments arranged by size: 2675 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2404 2436 6366 6398 2404 Size: 32 3930 32 2412 Fragments arranged by size: 3930 2412 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 4535 4535 Size: 6406 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI