(Circular) (Six-base) MAPSORT of: pFN21AB8645.seq Check: 9880 from: 1 to: 5503 pFN21AB8645 5503 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3313 278 Size: 53 83 186 936 1777 2468 Fragments arranged by size: 2468 1777 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2181 2846 1937 Size: 244 665 4594 Fragments arranged by size: 4594 665 244 Acc65I G'GTAC_C Cuts at: 2824 2824 Size: 5503 AclI AA'CG_TT Cuts at: 1563 3630 4003 1563 Size: 2067 373 3063 Fragments arranged by size: 3063 2067 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2324 2393 2548 3566 4950 5218 1632 Size: 692 69 155 1018 1384 268 1917 Fragments arranged by size: 1917 1384 1018 692 268 155 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5306 AflIII A'CryG_T Cuts at: 1203 2199 4402 5379 1203 Size: 996 2203 977 1327 Fragments arranged by size: 2203 1327 996 977 AgeI A'CCGG_T Cuts at: 4334 4334 Size: 5503 AhdI GACnn_n'nnGTC Cuts at: 4232 4232 Size: 5503 AleI CACnn'nnGTG Cuts at: 1166 2537 1166 Size: 1371 4132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2090 1184 Size: 906 4597 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2534 2566 2534 Size: 32 5471 AlwNI CAG_nnn'CTG Cuts at: 2353 3246 4818 2353 Size: 893 1572 3038 Fragments arranged by size: 3038 1572 893 ApaLI G'TGCA_C Cuts at: 2791 3560 4716 2791 Size: 769 1156 3578 Fragments arranged by size: 3578 1156 769 ApoI r'AATT_y Cuts at: 1493 2812 3038 3190 5299 1493 Size: 1319 226 152 2109 1697 Fragments arranged by size: 2109 1697 1319 226 152 AseI AT'TA_AT Cuts at: 160 1032 4057 160 Size: 872 3025 1606 Fragments arranged by size: 3025 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 AvaI C'yCGr_G Cuts at: 1870 1943 2088 2828 1870 Size: 73 145 740 4545 Fragments arranged by size: 4545 740 145 73 BamHI G'GATC_C Cuts at: 2833 3200 2833 Size: 367 5136 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 2455 2824 4279 618 Size: 325 217 627 294 374 369 1455 1842 Fragments arranged by size: 1842 1455 627 374 369 325 294 217 BanII G_rGCy'C Cuts at: 1391 2105 2597 2822 1391 Size: 714 492 225 4072 Fragments arranged by size: 4072 714 492 225 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2018 2275 928 Size: 971 119 257 4156 Fragments arranged by size: 4156 971 257 119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3694 3728 1903 Size: 34 1757 34 3678 Fragments arranged by size: 3678 1757 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2215 3395 4605 2215 Size: 1180 1210 3113 Fragments arranged by size: 3113 1210 1180 BclI T'GATC_A Cuts at: 1548 1548 Size: 5503 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4114 136 Size: 107 122 71 631 605 2442 1525 Fragments arranged by size: 2442 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2613 5498 2613 Size: 2885 2618 BlpI GC'TnA_GC Cuts at: 2920 2920 Size: 5503 Bme1580I G_kGCm'C Cuts at: 1790 2049 2460 2795 3564 4720 1790 Size: 259 411 335 769 1156 2573 Fragments arranged by size: 2573 1156 769 411 335 259 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5503 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3234 4192 442 Size: 416 53 83 399 24 1817 958 1753 Fragments arranged by size: 1817 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5503 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2375 4163 1239 Size: 1136 1788 2579 Fragments arranged by size: 2579 1788 1136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2720 3628 4493 4791 2720 Size: 908 865 298 3432 Fragments arranged by size: 3432 908 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2701 4166 882 Size: 617 1202 1465 2219 Fragments arranged by size: 2219 1465 1202 617 BsaAI yAC'GTr Cuts at: 493 1204 2557 3294 493 Size: 711 1353 737 2702 Fragments arranged by size: 2702 1353 737 711 BsaBI GATnn'nnATC Cuts at: 5497 5497 Size: 5503 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2634 2682 3310 3692 Size: 53 83 186 936 1101 48 628 382 Cuts at: 3692 4327 275 Size: 635 1451 Fragments arranged by size: 1451 1101 936 635 628 382 186 83 53 48 BsaWI w'CCGG_w Cuts at: 3936 4334 4608 4755 5166 3936 Size: 398 274 147 411 4273 Fragments arranged by size: 4273 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2348 2378 1178 Size: 30 165 30 945 30 4303 Fragments arranged by size: 4303 945 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5503 BseYI C'CCAG_C Cuts at: 2077 2717 4706 5202 2077 Size: 640 1989 496 2378 Fragments arranged by size: 2378 1989 640 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2017 2642 1163 Size: 573 281 625 4024 Fragments arranged by size: 4024 625 573 281 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2953 3714 4742 1540 Size: 275 186 952 761 1028 2301 Fragments arranged by size: 2301 1028 952 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2105 2562 2795 3564 3649 4720 2105 Size: 457 233 769 85 1071 2888 Fragments arranged by size: 2888 1071 769 457 233 85 BsmI GAATG_Cn' Cuts at: 3014 3107 3014 Size: 93 5410 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2105 2460 2562 2597 2795 2822 Size: 399 259 56 355 102 35 198 27 Cuts at: 2822 3564 3649 4720 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 355 259 198 102 85 56 35 27 BspEI T'CCGG_A Cuts at: 5166 5166 Size: 5503 BspHI T'CATG_A Cuts at: 3392 5122 3392 Size: 1730 3773 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2858 844 Size: 2014 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3390 1394 Size: 429 1567 3507 Fragments arranged by size: 3507 1567 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3998 4172 65 Size: 1183 2750 174 1396 Fragments arranged by size: 2750 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4147 4334 1700 Size: 110 2337 187 2869 Fragments arranged by size: 2869 2337 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5503 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5503 BssSI C'ACGA_G Cuts at: 2585 3563 4575 2585 Size: 978 1012 3513 Fragments arranged by size: 3513 1012 978 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5503 BstBI TT'CG_AA Cuts at: 4307 4307 Size: 5503 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5503 BstYI r'GATC_y Cuts at: 1972 2349 2613 2833 3200 3585 3602 5054 5140 Size: 377 264 220 367 385 17 1452 86 Cuts at: 5140 5152 5498 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 377 367 346 264 220 86 17 12 BstZ17I GTA'TAC Cuts at: 2182 2182 Size: 5503 BtgI C'CryG_G Cuts at: 513 1065 2191 2406 2434 513 Size: 552 1126 215 28 3582 Fragments arranged by size: 3582 1126 552 215 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2591 487 Size: 683 56 1365 3399 Fragments arranged by size: 3399 1365 683 56 BtsI GCAGTG_nn' Cuts at: 3019 3813 3833 3019 Size: 794 20 4689 Fragments arranged by size: 4689 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5503 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5468 DraI TTT'AAA Cuts at: 2807 2807 Size: 5503 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4510 809 Size: 507 3194 1802 Fragments arranged by size: 3194 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2128 2520 2571 Size: 54 1425 50 275 18 298 392 51 Cuts at: 2571 2950 3839 5468 8 Size: 379 889 1629 43 Fragments arranged by size: 1629 1425 889 392 379 298 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2950 1537 Size: 275 1138 4090 Fragments arranged by size: 4090 1138 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2567 3433 1353 Size: 1214 866 3423 Fragments arranged by size: 3423 1214 866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2411 4090 4464 4610 387 Size: 1064 138 822 1679 374 146 1280 Fragments arranged by size: 1679 1280 1064 822 374 146 138 EcoICRI GAG'CTC Cuts at: 2103 2103 Size: 5503 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2375 2393 2548 3566 4163 4950 Size: 393 692 51 18 155 1018 597 787 Cuts at: 4950 5218 1239 Size: 268 1524 Fragments arranged by size: 1524 1018 787 692 597 393 268 155 51 18 EcoO109I rG'GnC_Cy Cuts at: 2649 2755 2649 Size: 106 5397 EcoRI G'AATT_C Cuts at: 2812 3190 2812 Size: 378 5125 FspI TGC'GCA Cuts at: 2255 4009 2255 Size: 1754 3749 HaeII r_GCGC'y Cuts at: 1678 2338 4650 1678 Size: 660 2312 2531 Fragments arranged by size: 2531 2312 660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 4144 4176 4218 Size: 32 135 32 776 32 1764 32 42 Cuts at: 4218 4250 1373 Size: 32 2626 Fragments arranged by size: 2626 1764 776 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2725 2847 3093 669 Size: 988 281 787 122 246 3079 Fragments arranged by size: 3079 988 787 281 246 122 HindIII A'AGCT_T Cuts at: 748 748 Size: 5503 HpaI GTT'AAC Cuts at: 3093 3093 Size: 5503 KpnI G_GTAC'C Cuts at: 2828 2828 Size: 5503 MfeI C'AATT_G Cuts at: 3102 3102 Size: 5503 MluI A'CGCG_T Cuts at: 5379 5379 Size: 5503 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4617 4801 1305 Size: 3312 184 2007 Fragments arranged by size: 3312 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2573 10 Size: 54 1425 343 741 2940 Fragments arranged by size: 2940 1425 741 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2291 2537 3461 3820 3979 518 Size: 648 906 219 246 924 359 159 2042 Fragments arranged by size: 2042 924 906 648 359 246 219 159 MspA1I CmG'CkG Cuts at: 1606 2025 2193 2919 3596 4744 4989 1606 Size: 419 168 726 677 1148 245 2120 Fragments arranged by size: 2120 1148 726 677 419 245 168 NcoI C'CATG_G Cuts at: 513 1065 2406 513 Size: 552 1341 3610 Fragments arranged by size: 3610 1341 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5503 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5503 NotI GC'GGCC_GC Cuts at: 2950 2950 Size: 5503 NspI r_CATG'y Cuts at: 1132 1229 2203 2662 2861 4406 1132 Size: 97 974 459 199 1545 2229 Fragments arranged by size: 2229 1545 974 459 199 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5503 PciI A'CATG_T Cuts at: 2199 4402 2199 Size: 2203 3300 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5503 PfoI T'CCnGG_A Cuts at: 2217 2352 2217 Size: 135 5368 PmeI GTTT'AAAC Cuts at: 2807 2807 Size: 5503 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3572 3604 5085 5117 3572 Size: 32 1481 32 3958 Fragments arranged by size: 3958 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2755 2755 Size: 5503 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5503 PsiI TTA'TAA Cuts at: 3073 3073 Size: 5503 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2489 2521 2489 Size: 32 5471 PstI C_TGCA'G Cuts at: 830 2855 830 Size: 2025 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5503 SacI G_AGCT'C Cuts at: 2105 2105 Size: 5503 SacII CC_GC'GG Cuts at: 2194 2194 Size: 5503 SalI G'TCGA_C Cuts at: 1936 2845 1936 Size: 909 4594 SapI GCTCTTCn'nnn_ Cuts at: 2567 2567 Size: 5503 SbfI CC_TGCA'GG Cuts at: 2855 2855 Size: 5503 ScaI AGT'ACT Cuts at: 3751 5463 3751 Size: 1712 3791 SfcI C'TryA_G Cuts at: 826 1045 2851 3986 4667 4858 826 Size: 219 1806 1135 681 191 1471 Fragments arranged by size: 1806 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5503 SmaI CCC'GGG Cuts at: 1872 2830 1872 Size: 958 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2735 3607 4508 4770 820 Size: 197 926 792 872 901 262 1553 Fragments arranged by size: 1553 926 901 872 792 262 197 SnaBI TAC'GTA Cuts at: 493 3294 493 Size: 2801 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5503 SphI G_CATG'C Cuts at: 1132 2662 2861 1132 Size: 1530 199 3774 Fragments arranged by size: 3774 1530 199 SspI AAT'ATT Cuts at: 5 52 3427 5 Size: 47 3375 2081 Fragments arranged by size: 3375 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5503 StyI C'CwwG_G Cuts at: 513 1065 2385 2406 2751 2942 513 Size: 552 1320 21 345 191 3074 Fragments arranged by size: 3074 1320 552 345 191 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 2167 3530 3547 3700 3885 4330 Size: 1255 489 98 1363 17 153 185 445 Cuts at: 4330 325 Size: 1498 Fragments arranged by size: 1498 1363 1255 489 445 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 2739 3749 Size: 274 80 33 51 1443 168 594 1010 Cuts at: 3749 5461 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1010 594 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5463 5495 5463 Size: 32 5471 Tth111I GACn'n_nGTC Cuts at: 1316 2017 1316 Size: 701 4802 XbaI T'CTAG_A Cuts at: 2839 2839 Size: 5503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5503 XmaI C'CCGG_G Cuts at: 1870 2828 1870 Size: 958 4545 XmnI GAAnn'nnTTC Cuts at: 3632 3632 Size: 5503 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3311 276 Size: 53 83 186 936 1777 2468 Fragments arranged by size: 2468 1777 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI