(Circular) (Six-base) MAPSORT of: pFN21AB8631.seq Check: 4041 from: 1 to: 6097 pFN21AB8631 6097 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3385 3385 Size: 6097 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2493 2925 3118 3440 1937 Size: 556 432 193 322 4594 Fragments arranged by size: 4594 556 432 322 193 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2148 2607 2658 2940 3032 4160 5544 5812 Size: 516 459 51 282 92 1128 1384 268 Cuts at: 5812 1632 Size: 1917 Fragments arranged by size: 1917 1384 1128 516 459 282 268 92 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 4996 5973 1203 Size: 3793 977 1327 Fragments arranged by size: 3793 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 2612 1166 Size: 1446 4651 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2155 3193 1184 Size: 971 1038 4088 Fragments arranged by size: 4088 1038 971 AlwNI CAG_nnn'CTG Cuts at: 3840 5412 3840 Size: 1572 4525 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2643 3406 3632 3784 5893 1493 Size: 1150 763 226 152 2109 1697 Fragments arranged by size: 2109 1697 1150 763 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2181 3422 1870 Size: 73 238 1241 4545 Fragments arranged by size: 4545 1241 238 73 BamHI G'GATC_C Cuts at: 2230 3427 3794 2230 Size: 1197 367 4533 Fragments arranged by size: 4533 1197 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3418 4873 618 Size: 325 217 627 1631 1455 1842 Fragments arranged by size: 1842 1631 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2094 2218 2391 3416 1391 Size: 703 124 173 1025 4072 Fragments arranged by size: 4072 1025 703 173 124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 928 Size: 971 199 4927 Fragments arranged by size: 4927 971 199 BbvCI CC'TCA_GC Cuts at: 3312 3312 Size: 6097 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4708 136 Size: 107 122 71 631 605 3036 1525 Fragments arranged by size: 3036 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 4158 5314 1790 Size: 2368 1156 2573 Fragments arranged by size: 2573 2368 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6097 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2575 3828 4786 Size: 416 53 83 399 24 1158 1253 958 Cuts at: 4786 442 Size: 1753 Fragments arranged by size: 1753 1253 1158 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2331 4757 1239 Size: 1092 2426 2579 Fragments arranged by size: 2579 2426 1092 Bpu10I CC'TnA_GC Cuts at: 3312 3312 Size: 6097 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2492 3076 4222 5087 5385 2492 Size: 584 1146 865 298 3204 Fragments arranged by size: 3204 1146 865 584 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 2783 4760 882 Size: 617 504 780 1977 2219 Fragments arranged by size: 2219 1977 780 617 504 BsaAI yAC'GTr Cuts at: 493 1204 3103 3888 493 Size: 711 1899 785 2702 Fragments arranged by size: 2702 1899 785 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3904 4286 4921 275 Size: 53 83 186 936 2371 382 635 1451 Fragments arranged by size: 2371 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2076 2106 2275 2305 3060 Size: 30 165 30 673 30 169 30 755 Cuts at: 3060 3090 1178 Size: 30 4185 Fragments arranged by size: 4185 755 673 169 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2564 1186 Size: 1378 4719 BseYI C'CCAG_C Cuts at: 2055 2076 2221 5300 5796 2055 Size: 21 145 3079 496 2356 Fragments arranged by size: 3079 2356 496 145 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2504 1163 Size: 573 768 4756 Fragments arranged by size: 4756 768 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3547 4308 5336 1540 Size: 275 186 1546 761 1028 2301 Fragments arranged by size: 2301 1546 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4158 4243 5314 4158 Size: 85 1071 4941 Fragments arranged by size: 4941 1071 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2218 2391 3416 4158 4243 5314 Size: 399 304 124 173 1025 742 85 1071 Cuts at: 5314 1391 Size: 2174 Fragments arranged by size: 2174 1071 1025 742 399 304 173 124 85 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2526 3385 3452 844 Size: 1682 859 67 3489 Fragments arranged by size: 3489 1682 859 67 BsrBI CCG'CTC Cuts at: 1394 1823 3984 1394 Size: 429 2161 3507 Fragments arranged by size: 3507 2161 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2139 4592 4766 65 Size: 1183 891 2453 174 1396 Fragments arranged by size: 2453 1396 1183 891 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2012 2498 4741 4928 1700 Size: 110 202 486 2243 187 2869 Fragments arranged by size: 2869 2243 486 202 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 2527 794 Size: 1733 4364 BstBI TT'CG_AA Cuts at: 2894 4901 2894 Size: 2007 4090 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2039 2230 3130 3427 3794 4179 4196 5648 Size: 67 191 900 297 367 385 17 1452 Cuts at: 5648 5734 5746 6092 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 900 385 367 346 297 191 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2657 2657 Size: 6097 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 513 Size: 552 939 114 4492 Fragments arranged by size: 4492 939 552 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 2137 3613 4407 4427 2137 Size: 1476 794 20 3807 Fragments arranged by size: 3807 1476 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2378 2933 5104 809 Size: 507 1062 555 2171 1802 Fragments arranged by size: 2171 1802 1062 555 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2293 3212 3544 Size: 54 1425 50 275 18 463 919 332 Cuts at: 3544 4433 6062 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 919 889 463 332 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2898 4027 1353 Size: 705 840 1129 3423 Fragments arranged by size: 3423 1129 840 705 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2331 2607 2658 2940 3032 4160 Size: 393 516 183 276 51 282 92 1128 Cuts at: 4160 4757 5544 5812 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1128 787 597 516 393 282 276 268 183 92 51 EcoO109I rG'GnC_Cy Cuts at: 2186 2286 2186 Size: 100 5997 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3308 3308 Size: 6097 FspI TGC'GCA Cuts at: 2732 4603 2732 Size: 1871 4226 FspAI rTGC'GCAy Cuts at: 2732 2732 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 5244 1678 Size: 3566 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2076 2108 2435 2467 2782 Size: 32 135 32 504 32 327 32 315 Cuts at: 2782 2814 2899 2931 3060 3092 4738 4770 4812 Size: 32 85 32 129 32 1646 32 42 Cuts at: 4812 4844 1373 Size: 32 2626 Fragments arranged by size: 2626 1646 504 327 315 135 129 85 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2809 3441 3687 669 Size: 988 281 871 632 246 3079 Fragments arranged by size: 3079 988 871 632 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5211 5395 1305 Size: 3906 184 2007 Fragments arranged by size: 3906 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2295 3214 10 Size: 54 1425 343 463 919 2893 Fragments arranged by size: 2893 1425 919 463 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 2612 4055 4414 4573 518 Size: 648 1248 198 1443 359 159 2042 Fragments arranged by size: 2042 1443 1248 648 359 198 159 MspA1I CmG'CkG Cuts at: 1606 2076 2221 2351 2448 3513 4190 5338 5583 Size: 470 145 130 97 1065 677 1148 245 Cuts at: 5583 1606 Size: 2120 Fragments arranged by size: 2120 1148 1065 677 470 245 145 130 97 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2731 3455 5000 1132 Size: 97 1502 724 1545 2229 Fragments arranged by size: 2229 1545 1502 724 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2186 2286 2186 Size: 100 5997 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PstI C_TGCA'G Cuts at: 830 2144 2862 3169 3382 3449 830 Size: 1314 718 307 213 67 3478 Fragments arranged by size: 3478 1314 718 307 213 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 2076 2351 2448 2076 Size: 275 97 5725 Fragments arranged by size: 5725 275 97 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 3053 4345 6057 3053 Size: 1292 1712 3093 Fragments arranged by size: 3093 1712 1292 SfcI C'TryA_G Cuts at: 826 1045 2140 2172 2858 3165 3378 3445 4580 Size: 219 1095 32 686 307 213 67 1135 Cuts at: 4580 5261 5452 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1095 686 681 307 219 213 191 67 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2507 3055 4201 5102 5364 820 Size: 197 926 564 548 1146 901 262 1553 Fragments arranged by size: 1553 1146 926 901 564 548 262 197 SnaBI TAC'GTA Cuts at: 493 3103 3888 493 Size: 2610 785 2702 Fragments arranged by size: 2702 2610 785 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 2731 3455 1132 Size: 1599 724 3774 Fragments arranged by size: 3774 1599 724 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2004 3536 513 Size: 552 939 1532 3074 Fragments arranged by size: 3074 1532 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2443 4124 4141 4294 4479 4924 325 Size: 1255 863 1681 17 153 185 445 1498 Fragments arranged by size: 1681 1498 1255 863 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 3051 3333 Size: 274 80 33 51 1443 300 774 282 Cuts at: 3333 4343 6055 96 Size: 1010 1712 138 Fragments arranged by size: 1712 1443 1010 774 300 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2275 2307 6057 6089 2275 Size: 32 3750 32 2283 Fragments arranged by size: 3750 2283 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2218 2218 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI