(Circular) (Six-base) MAPSORT of: pFN21AB8629.seq Check: 8583 from: 1 to: 6298 pFN21AB8629 6298 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4108 278 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3303 3641 1937 Size: 1366 338 4594 Fragments arranged by size: 4594 1366 338 Acc65I G'GTAC_C Cuts at: 3619 3619 Size: 6298 AclI AA'CG_TT Cuts at: 1563 3597 4425 4798 1563 Size: 2034 828 373 3063 Fragments arranged by size: 3063 2034 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3104 3140 4361 5745 6013 1632 Size: 1472 36 1221 1384 268 1917 Fragments arranged by size: 1917 1472 1384 1221 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6101 AflIII A'CryG_T Cuts at: 1203 2260 2892 2922 2928 2997 5197 6174 1203 Size: 1057 632 30 6 69 2200 977 1327 Fragments arranged by size: 2200 1327 1057 977 632 69 30 6 AgeI A'CCGG_T Cuts at: 5129 5129 Size: 6298 AhdI GACnn_n'nnGTC Cuts at: 5027 5027 Size: 6298 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6298 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6298 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2247 2279 2247 Size: 32 6266 AlwNI CAG_nnn'CTG Cuts at: 3110 4041 5613 3110 Size: 931 1572 3795 Fragments arranged by size: 3795 1572 931 ApaLI G'TGCA_C Cuts at: 4355 5511 4355 Size: 1156 5142 ApoI r'AATT_y Cuts at: 1493 3092 3470 3557 3607 3833 3985 6094 1493 Size: 1599 378 87 50 226 152 2109 1697 Fragments arranged by size: 2109 1697 1599 378 226 152 87 50 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 3623 1870 Size: 73 1680 4545 Fragments arranged by size: 4545 1680 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2178 2211 2178 Size: 33 6265 BamHI G'GATC_C Cuts at: 3628 3995 3628 Size: 367 5931 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 3033 3619 5074 618 Size: 325 217 627 300 946 586 1455 1842 Fragments arranged by size: 1842 1455 946 627 586 325 300 217 BanII G_rGCy'C Cuts at: 1391 3617 1391 Size: 2226 4072 BbeI G_GCGC'C Cuts at: 3037 3037 Size: 6298 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2074 3063 3225 3323 928 Size: 971 175 989 162 98 3903 Fragments arranged by size: 3903 989 971 175 162 98 BbvCI CC'TCA_GC Cuts at: 2092 2092 Size: 6298 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4489 4523 1903 Size: 34 2552 34 3678 Fragments arranged by size: 3678 2552 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4190 5400 4190 Size: 1210 5088 BclI T'GATC_A Cuts at: 1548 2382 1548 Size: 834 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4909 136 Size: 107 122 71 631 605 3237 1525 Fragments arranged by size: 3237 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6293 6293 Size: 6298 BlpI GC'TnA_GC Cuts at: 2825 3715 2825 Size: 890 5408 Bme1580I G_kGCm'C Cuts at: 1790 2922 4359 5515 1790 Size: 1132 1437 1156 2573 Fragments arranged by size: 2573 1437 1156 1132 BmgBI CAC'GTC Cuts at: 1318 2895 2998 1318 Size: 1577 103 4618 Fragments arranged by size: 4618 1577 103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3254 4029 4987 Size: 416 53 83 399 24 1837 775 958 Cuts at: 4987 442 Size: 1753 Fragments arranged by size: 1837 1753 958 775 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2193 3263 4958 1239 Size: 954 1070 1695 2579 Fragments arranged by size: 2579 1695 1070 954 Bpu10I CC'TnA_GC Cuts at: 2092 2092 Size: 6298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2034 4423 5288 5586 2034 Size: 2389 865 298 2746 Fragments arranged by size: 2746 2389 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4961 882 Size: 617 3462 2219 Fragments arranged by size: 3462 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2923 4089 493 Size: 711 1719 1166 2702 Fragments arranged by size: 2702 1719 1166 711 BsaBI GATnn'nnATC Cuts at: 3239 6292 3239 Size: 3053 3245 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3034 4105 4487 5122 Size: 53 83 186 936 1501 1071 382 635 Cuts at: 5122 275 Size: 1451 Fragments arranged by size: 1501 1451 1071 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4731 5129 5403 5550 5961 4731 Size: 398 274 147 411 5068 Fragments arranged by size: 5068 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6073 Fragments arranged by size: 6073 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2505 1186 Size: 1319 4979 BseYI C'CCAG_C Cuts at: 2616 2641 2797 2830 5501 5997 2616 Size: 25 156 33 2671 496 2917 Fragments arranged by size: 2917 2671 496 156 33 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2971 1163 Size: 573 1235 4490 Fragments arranged by size: 4490 1235 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3748 4509 5537 1540 Size: 275 186 1747 761 1028 2301 Fragments arranged by size: 2301 1747 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4359 4444 5515 4359 Size: 85 1071 5142 Fragments arranged by size: 5142 1071 85 BsmI GAATG_Cn' Cuts at: 3421 3809 3902 3421 Size: 388 93 5817 Fragments arranged by size: 5817 388 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3031 3031 Size: 6298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2922 3617 4359 4444 5515 1391 Size: 399 1132 695 742 85 1071 2174 Fragments arranged by size: 2174 1132 1071 742 695 399 85 BspEI T'CCGG_A Cuts at: 5961 5961 Size: 6298 BspHI T'CATG_A Cuts at: 4187 5917 4187 Size: 1730 4568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2829 3315 3653 844 Size: 1985 486 338 3489 Fragments arranged by size: 3489 1985 486 338 BsrBI CCG'CTC Cuts at: 1394 1823 4185 1394 Size: 429 2362 3507 Fragments arranged by size: 3507 2362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2559 3561 4793 4967 65 Size: 1183 1311 1002 1232 174 1396 Fragments arranged by size: 1396 1311 1232 1183 1002 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4942 5129 1700 Size: 110 3132 187 2869 Fragments arranged by size: 3132 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2925 96 Size: 2829 3469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6298 BssSI C'ACGA_G Cuts at: 3028 4358 5370 3028 Size: 1330 1012 3956 Fragments arranged by size: 3956 1330 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6298 BstBI TT'CG_AA Cuts at: 3127 5102 3127 Size: 1975 4323 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6298 BstYI r'GATC_y Cuts at: 1972 3628 3995 4380 4397 5849 5935 5947 6293 Size: 1656 367 385 17 1452 86 12 346 Cuts at: 6293 1972 Size: 1977 Fragments arranged by size: 1977 1656 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2583 513 Size: 552 939 579 4228 Fragments arranged by size: 4228 939 579 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2335 487 Size: 683 56 1109 4450 Fragments arranged by size: 4450 1109 683 56 BtsI GCAGTG_nn' Cuts at: 3814 4608 4628 3814 Size: 794 20 5484 Fragments arranged by size: 5484 794 20 ClaI AT'CG_AT Cuts at: 1554 3243 1554 Size: 1689 4609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6263 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DraIII CAC_nnn'GTG Cuts at: 2701 2701 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5305 809 Size: 507 3989 1802 Fragments arranged by size: 3989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2476 3745 4634 Size: 54 1425 50 275 18 646 1269 889 Cuts at: 4634 6263 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1269 889 646 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3745 1537 Size: 275 1933 4090 Fragments arranged by size: 4090 1933 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4228 1353 Size: 2875 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4885 5259 5405 387 Size: 1064 138 3296 374 146 1280 Fragments arranged by size: 3296 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2193 3104 3140 3263 4361 4958 5745 Size: 393 561 911 36 123 1098 597 787 Cuts at: 5745 6013 1239 Size: 268 1524 Fragments arranged by size: 1524 1098 911 787 597 561 393 268 123 36 EcoRI G'AATT_C Cuts at: 3470 3557 3607 3985 3470 Size: 87 50 378 5783 Fragments arranged by size: 5783 378 87 50 FspI TGC'GCA Cuts at: 4804 4804 Size: 6298 HaeII r_GCGC'y Cuts at: 1678 3037 5445 1678 Size: 1359 2408 2531 Fragments arranged by size: 2531 2408 1359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 4939 4971 5013 Size: 32 135 32 473 32 2862 32 42 Cuts at: 5013 5045 1373 Size: 32 2626 Fragments arranged by size: 2862 2626 473 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3304 3642 3888 669 Size: 988 281 1366 338 246 3079 Fragments arranged by size: 3079 1366 988 338 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6298 HpaI GTT'AAC Cuts at: 3888 3888 Size: 6298 KasI G'GCGC_C Cuts at: 3033 3033 Size: 6298 KpnI G_GTAC'C Cuts at: 3623 3623 Size: 6298 MfeI C'AATT_G Cuts at: 3897 3897 Size: 6298 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1996 5412 5596 1305 Size: 691 3416 184 2007 Fragments arranged by size: 3416 2007 691 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2478 10 Size: 54 1425 343 646 3830 Fragments arranged by size: 3830 1425 646 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2843 4256 4615 4774 518 Size: 648 1677 1413 359 159 2042 Fragments arranged by size: 2042 1677 1413 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2438 2641 2668 2797 3714 4391 5539 5784 Size: 832 203 27 129 917 677 1148 245 Cuts at: 5784 1606 Size: 2120 Fragments arranged by size: 2120 1148 917 832 677 245 203 129 27 NarI GG'CG_CC Cuts at: 3034 3034 Size: 6298 NcoI C'CATG_G Cuts at: 513 1065 2004 2583 513 Size: 552 939 579 4228 Fragments arranged by size: 4228 939 579 552 NdeI CA'TA_TG Cuts at: 387 2788 387 Size: 2401 3897 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6298 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NspI r_CATG'y Cuts at: 1132 1229 2264 2932 3656 5201 1132 Size: 97 1035 668 724 1545 2229 Fragments arranged by size: 2229 1545 1035 724 668 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6298 PciI A'CATG_T Cuts at: 2260 2928 5197 2260 Size: 668 2269 3361 Fragments arranged by size: 3361 2269 668 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6298 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PmlI CAC'GTG Cuts at: 2923 2923 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6298 PsiI TTA'TAA Cuts at: 2466 3868 2466 Size: 1402 4896 PstI C_TGCA'G Cuts at: 830 2902 3442 3523 3650 830 Size: 2072 540 81 127 3478 Fragments arranged by size: 3478 2072 540 127 81 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 PvuII CAG'CTG Cuts at: 2438 2641 2668 2797 2438 Size: 203 27 129 5939 Fragments arranged by size: 5939 203 129 27 SalI G'TCGA_C Cuts at: 1936 3302 3640 1936 Size: 1366 338 4594 Fragments arranged by size: 4594 1366 338 SbfI CC_TGCA'GG Cuts at: 3650 3650 Size: 6298 ScaI AGT'ACT Cuts at: 4546 6258 4546 Size: 1712 4586 SfcI C'TryA_G Cuts at: 826 1045 2152 2496 2898 3324 3438 3519 3646 Size: 219 1107 344 402 426 114 81 127 Cuts at: 3646 4781 5462 5653 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1107 681 426 402 344 219 191 127 114 81 SfoI GGC'GCC Cuts at: 3035 3035 Size: 6298 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6298 SmaI CCC'GGG Cuts at: 1872 3625 1872 Size: 1753 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2049 4402 5303 5565 820 Size: 197 926 106 2353 901 262 1553 Fragments arranged by size: 2353 1553 926 901 262 197 106 SnaBI TAC'GTA Cuts at: 493 4089 493 Size: 3596 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6298 SphI G_CATG'C Cuts at: 1132 3656 1132 Size: 2524 3774 SspI AAT'ATT Cuts at: 5 52 4222 5 Size: 47 4170 2081 Fragments arranged by size: 4170 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6298 StyI C'CwwG_G Cuts at: 513 1065 2004 2344 2583 3737 513 Size: 552 939 340 239 1154 3074 Fragments arranged by size: 3074 1154 939 552 340 239 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2990 4325 4342 4495 4680 5125 325 Size: 1255 1410 1335 17 153 185 445 1498 Fragments arranged by size: 1498 1410 1335 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2186 2925 4544 Size: 274 80 33 51 1443 209 739 1619 Cuts at: 4544 6256 96 Size: 1712 138 Fragments arranged by size: 1712 1619 1443 739 274 209 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2062 2094 3393 3425 6258 6290 2062 Size: 32 1299 32 2833 32 2070 Fragments arranged by size: 2833 2070 1299 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3299 1316 Size: 1983 4315 XbaI T'CTAG_A Cuts at: 3634 3634 Size: 6298 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2327 2590 2327 Size: 263 6035 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 3623 1870 Size: 1753 4545 XmnI GAAnn'nnTTC Cuts at: 4427 4427 Size: 6298 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4106 276 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI