Restriction map for
pFN21AB8624
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvrII BalI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaAI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstXI BstZ17I ClaI DraI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacI SacII
SalI SbfI ScaI SexAI SfiI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 516 GACGTC GACGT^C
AccI 184 GTAGAC GT^[AC][GT]AC
AccII 172 CGCG CG^CG
AfaI 333 GTAC GT^AC
AocI 125 CCTCAGG CC^T[ACGT]AGG
AviII 773 TGCGCA TGC^GCA
BamHI 905 GGATCC G^GATCC
BcgI 566 GCACCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 134 CTAG C^TAG
BglI 313 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 411 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 685 CATTGC GCAATG(2/0)
BstBI 64 TTCGAA TT^CGAA
BstEII 1032 GGTGACC G^GT[ACGT]ACC
BstPI 1032 GGTGACC G^GT[ACGT]ACC
BstUI 172 CGCG CG^CG
BstYI 905 GGATCC [GA]^GATC[CT]
Bsu36I 125 CCTCAGG CC^T[ACGT]AGG
CpoI 799 CGGACCG CG^G[AT]CCG
DraIII 889 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1035 GACCCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 125 CCTCAGG CC^T[ACGT]AGG
EcoNI 168 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1032 GGTGACC G^GT[ACGT]ACC
EcoT14I 504 CCATGG C^C[AT][AT]GG
FspI 773 TGCGCA TGC^GCA
HgaI 187 GACGC GACGC(5/10)
HincII 427 GTCAAC GT[CT]^[GA]AC
HindII 427 GTCAAC GT[CT]^[GA]AC
MaeI 134 CTAG C^TAG
MaeII 517 ACGT A^CGT
MamI 411 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 905 GGATCC [GA]^GATC[CT]
MseI 254 TTAA T^TAA
MslI 543 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 172 CGCG CG^CG
NcoI 504 CCATGG C^CATGG
NspI 854 ACATGC [GA]CATG^[CT]
NspV 64 TTCGAA TT^CGAA
PflMI 888 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 950 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 333 GTAC GT^AC
RsrII 799 CGGACCG CG^G[AT]CCG
SapI 858 GCTCTTC GCTCTTC(1/4)
SfuI 64 TTCGAA TT^CGAA
SgrAI 17 CGCCGGCG C[GA]^CCGG[CT]G
StyI 504 CCATGG C^C[AT][AT]GG
Tru9I 254 TTAA T^TAA
Tsp509I 444 AATT ^AATT
Van91I 888 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 767 CCACACTGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 905 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 807 GGGCCC GGGCC^C
924 GGGCCC
BanII 807 GGGCCC G[GA]GC[CT]^C
924 GGGCCC
BbeI 15 GGCGCC GGCGC^C
28 GGCGCC
BbsI 513 GAAGAC GAAGAC(2/6)
478 GTCTTC
BcnI 48 CCCGG CC^[GC]GG
534 CCGGG
BlpI 89 GCTCAGC GC^T[ACGT]AGC
264 GCTGAGC
Bpu1102I 89 GCTCAGC GC^T[ACGT]AGC
264 GCTGAGC
BpuAI 513 GAAGAC GAAGAC(2/6)
478 GTCTTC
BseRI 826 CTCCTC GAGGAG(10/8)
220 GAGGAG
BsmFI 258 GGGAC GGGAC(10/14)
952 GTCCC
BsrFI 18 GCCGGC [GA]^CCGG[CT]
269 GCCGGC
BsrI 581 ACTGG ACTGG(1/-1)
918 ACTGG
BstAPI 196 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
205 GCAGCTGCTGC
CelII 89 GCTCAGC GC^T[ACGT]AGC
264 GCTGAGC
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
269 GCCGGC
DsaI 504 CCATGG C^C[GA][CT]GG
803 CCGTGG
FauI 936 CCCGC CCCGC(4/6)
22 GCGGG
HinfI 403 GAGTC G^A[ACGT]TC
590 GAGTC
KasI 15 GGCGCC G^GCGCC
28 GGCGCC
MaeIII 1033 GTGAC ^GT[ACGT]AC
1044 GTCAC
NaeI 18 GCCGGC GCC^GGC
269 GCCGGC
NarI 15 GGCGCC GG^CGCC
28 GGCGCC
NciI 48 CCCGG CC^[GC]GG
534 CCGGG
NgoMIV 18 GCCGGC G^CCGGC
269 GCCGGC
PleI 403 GAGTC GAGTC(4/5)
590 GAGTC
PstI 646 CTGCAG CTGCA^G
1039 CTGCAG
SfoI 15 GGCGCC GGC^GCC
28 GGCGCC
Tsp45I 1033 GTGAC ^GT[GC]AC
1044 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
AvaII BanI BbvI BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmAI
Bsp1286I BspMI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
Ksp632I MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI TspRI
TthHB8I