(Circular) (Six-base) MAPSORT of: pFN21AB8624.seq Check: 7712 from: 1 to: 5755 pFN21AB8624 5755 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2269 2444 2582 2644 2269 Size: 175 138 62 5380 Fragments arranged by size: 5380 175 138 62 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2525 3565 278 Size: 53 83 186 936 989 1040 2468 Fragments arranged by size: 2468 1040 989 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2190 3098 1937 Size: 253 908 4594 Fragments arranged by size: 4594 908 253 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2241 2314 3818 5202 5470 1632 Size: 609 73 1504 1384 268 1917 Fragments arranged by size: 1917 1504 1384 609 268 73 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 3052 4654 5631 1203 Size: 1849 1602 977 1327 Fragments arranged by size: 1849 1602 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2222 2254 2222 Size: 32 5723 AlwNI CAG_nnn'CTG Cuts at: 2173 2216 2827 3498 5070 2173 Size: 43 611 671 1572 2858 Fragments arranged by size: 2858 1572 671 611 43 ApaI G_GGCC'C Cuts at: 2816 2933 2816 Size: 117 5638 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 3064 3290 3442 5551 1493 Size: 1571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1571 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 BamHI G'GATC_C Cuts at: 2910 3085 3452 2910 Size: 175 367 5213 Fragments arranged by size: 5213 367 175 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2033 2546 2570 2840 Size: 325 217 627 233 13 513 24 270 Cuts at: 2840 3076 4531 618 Size: 236 1455 1842 Fragments arranged by size: 1842 1455 627 513 325 270 236 233 217 24 13 BanII G_rGCy'C Cuts at: 1391 2816 2933 3074 1391 Size: 1425 117 141 4072 Fragments arranged by size: 4072 1425 141 117 BbeI G_GCGC'C Cuts at: 2024 2037 2024 Size: 13 5742 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2476 2525 928 Size: 971 577 49 4158 Fragments arranged by size: 4158 971 577 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2560 2594 3946 3980 1903 Size: 34 623 34 1352 34 3678 Fragments arranged by size: 3678 1352 623 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2324 4366 136 Size: 107 122 71 631 605 652 2042 1525 Fragments arranged by size: 2042 1525 652 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 2095 2270 3172 2095 Size: 175 902 4678 Fragments arranged by size: 4678 902 175 Bme1580I G_kGCm'C Cuts at: 1790 2549 2561 2816 2933 3816 4972 1790 Size: 759 12 255 117 883 1156 2573 Fragments arranged by size: 2573 1156 883 759 255 117 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2305 2426 2945 4415 1239 Size: 1066 121 519 1470 2579 Fragments arranged by size: 2579 1470 1066 519 121 Bpu10I CC'TnA_GC Cuts at: 2385 2385 Size: 5755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3880 4745 5043 3880 Size: 865 298 4592 Fragments arranged by size: 4592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 2420 5749 2420 Size: 3329 2426 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 2034 2522 3562 Size: 53 83 186 936 488 13 488 1040 Cuts at: 3562 3944 4579 275 Size: 382 635 1451 Fragments arranged by size: 1451 1040 936 635 488 488 382 186 83 53 13 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2822 1186 Size: 1054 582 4119 Fragments arranged by size: 4119 1054 582 BseYI C'CCAG_C Cuts at: 2303 2590 2668 4958 5454 2303 Size: 287 78 2290 496 2604 Fragments arranged by size: 2604 2290 496 287 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3816 3901 4972 3816 Size: 85 1071 4599 Fragments arranged by size: 4599 1071 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2549 2561 2816 2933 3074 3816 3901 Size: 399 759 12 255 117 141 742 85 Cuts at: 3901 4972 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 759 742 399 255 141 117 85 12 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2269 2444 2582 2644 3110 844 Size: 1425 175 138 62 466 3489 Fragments arranged by size: 3489 1425 466 175 138 62 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2689 4250 4424 65 Size: 1183 1441 1561 174 1396 Fragments arranged by size: 1561 1441 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 2274 4399 4586 1700 Size: 110 213 251 2125 187 2869 Fragments arranged by size: 2869 2125 251 213 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 2207 2216 794 Size: 1413 9 4333 Fragments arranged by size: 4333 1413 9 BstBI TT'CG_AA Cuts at: 2070 4559 2070 Size: 2489 3266 BstEII G'GTnAC_C Cuts at: 1184 3037 1184 Size: 1853 3902 BstYI r'GATC_y Cuts at: 1972 2910 3085 3452 3837 3854 5306 5392 5404 Size: 938 175 367 385 17 1452 86 12 Cuts at: 5404 5750 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 938 385 367 346 175 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2131 2131 Size: 5755 BtgI C'CryG_G Cuts at: 513 1065 2004 2509 2808 513 Size: 552 939 505 299 3460 Fragments arranged by size: 3460 939 552 505 299 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 2663 2774 2796 3271 4065 4085 2663 Size: 111 22 475 794 20 4333 Fragments arranged by size: 4333 794 475 111 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DraIII CAC_nnn'GTG Cuts at: 2899 2899 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3046 4762 809 Size: 507 1730 1716 1802 Fragments arranged by size: 1802 1730 1716 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720 Size: 54 1425 50 275 18 1372 889 1629 Cuts at: 5720 8 Size: 43 Fragments arranged by size: 1629 1425 1372 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2217 2741 2750 2870 3685 1353 Size: 864 524 9 120 815 3423 Fragments arranged by size: 3423 864 815 524 120 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2103 4342 4716 4862 387 Size: 1064 138 514 2239 374 146 1280 Fragments arranged by size: 2239 1280 1064 514 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2241 2305 2314 2426 2945 3818 4415 Size: 393 609 64 9 112 519 873 597 Cuts at: 4415 5202 5470 1239 Size: 787 268 1524 Fragments arranged by size: 1524 873 787 609 597 519 393 268 112 64 9 EcoNI CCTnn'n_nnAGG Cuts at: 2177 2177 Size: 5755 EcoO109I rG'GnC_Cy Cuts at: 2325 2813 2929 2930 2956 2325 Size: 488 116 1 26 5124 Fragments arranged by size: 5124 488 116 26 1 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FspI TGC'GCA Cuts at: 2780 4261 2780 Size: 1481 4274 HaeII r_GCGC'y Cuts at: 1678 2024 2037 2173 4902 1678 Size: 346 13 136 2729 2531 Fragments arranged by size: 2729 2531 346 136 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 4396 4428 4470 Size: 32 135 32 542 32 2250 32 42 Cuts at: 4470 4502 1373 Size: 32 2626 Fragments arranged by size: 2626 2250 542 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2434 3099 3345 669 Size: 988 281 496 665 246 3079 Fragments arranged by size: 3079 988 665 496 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KasI G'GCGC_C Cuts at: 2020 2033 2020 Size: 13 5742 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4869 5053 1305 Size: 3564 184 2007 Fragments arranged by size: 3564 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3933 Fragments arranged by size: 3933 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 3713 4072 4231 518 Size: 648 1386 1161 359 159 2042 Fragments arranged by size: 2042 1386 1161 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2099 2204 2213 2269 2824 3171 3848 Size: 412 81 105 9 56 555 347 677 Cuts at: 3848 4996 5241 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 555 412 347 245 105 81 56 9 NaeI GCC'GGC Cuts at: 2025 2276 2025 Size: 251 5504 NarI GG'CG_CC Cuts at: 2021 2034 2021 Size: 13 5742 NcoI C'CATG_G Cuts at: 513 1065 2004 2509 513 Size: 552 939 505 3759 Fragments arranged by size: 3759 939 552 505 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NgoMIV G'CCGG_C Cuts at: 2023 2274 2023 Size: 251 5504 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2863 3113 4658 1132 Size: 97 1634 250 1545 2229 Fragments arranged by size: 2229 1634 1545 250 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2899 1108 Size: 1791 3964 PfoI T'CCnGG_A Cuts at: 2403 2538 2913 2403 Size: 135 375 5245 Fragments arranged by size: 5245 375 135 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2956 2956 Size: 5755 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PspOMI G'GGCC_C Cuts at: 2812 2929 2812 Size: 117 5638 PstI C_TGCA'G Cuts at: 830 2655 3048 3107 830 Size: 1825 393 59 3478 Fragments arranged by size: 3478 1825 393 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2099 2204 2213 2269 2099 Size: 105 9 56 5585 Fragments arranged by size: 5585 105 56 9 RsrII CG'GwC_CG Cuts at: 2805 2805 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SanDI GG'GwC_CC Cuts at: 2956 2956 Size: 5755 SapI GCTCTTCn'nnn_ Cuts at: 2870 2870 Size: 5755 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2013 2651 3044 3103 4238 4919 5110 Size: 219 968 638 393 59 1135 681 191 Cuts at: 5110 826 Size: 1471 Fragments arranged by size: 1471 1135 968 681 638 393 219 191 59 SfoI GGC'GCC Cuts at: 2022 2035 2022 Size: 13 5742 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 2023 1700 Size: 323 5432 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3859 4760 5022 820 Size: 197 926 1916 901 262 1553 Fragments arranged by size: 1916 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2004 2509 3194 513 Size: 552 939 505 685 3074 Fragments arranged by size: 3074 939 685 552 505 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2144 2750 3782 3799 3952 4137 4582 Size: 1255 564 606 1032 17 153 185 445 Cuts at: 4582 325 Size: 1498 Fragments arranged by size: 1498 1255 1032 606 564 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2779 2779 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2523 3563 276 Size: 53 83 186 936 989 1040 2468 Fragments arranged by size: 2468 1040 989 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SacI SacII SexAI SfiI SrfI SwaI