Restriction map for
pFN21AB8624
(Linear) (Six-base) MAPSORT of: KIBB8624.seq Check: 7083 from: 1 to: 1050
KIBB8624.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 264 439 577 639 1050
Size: 264 175 138 62 411
Fragments arranged by size:
411 264 175 138 62
AatII G_ACGT'C
Cuts at: 0 520 1050
Size: 520 530
AccI GT'mk_AC
Cuts at: 0 185 1050
Size: 185 865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 309 1050
Size: 236 73 741
Fragments arranged by size:
741 236 73
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 1050
Size: 217 32 801
Fragments arranged by size:
801 217 32
AlwNI CAG_nnn'CTG
Cuts at: 0 168 211 822 1050
Size: 168 43 611 228
Fragments arranged by size:
611 228 168 43
ApaI G_GGCC'C
Cuts at: 0 811 928 1050
Size: 811 117 122
Fragments arranged by size:
811 122 117
BamHI G'GATC_C
Cuts at: 0 905 1050
Size: 905 145
BanI G'GyrC_C
Cuts at: 0 15 28 541 565 835 1050
Size: 15 13 513 24 270 215
Fragments arranged by size:
513 270 215 24 15 13
BanII G_rGCy'C
Cuts at: 0 811 928 1050
Size: 811 117 122
Fragments arranged by size:
811 122 117
BbeI G_GCGC'C
Cuts at: 0 19 32 1050
Size: 19 13 1018
Fragments arranged by size:
1018 19 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 471 520 1050
Size: 471 49 530
Fragments arranged by size:
530 471 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 555 589 1050
Size: 555 34 461
Fragments arranged by size:
555 461 34
BglI GCCn_nnn'nGGC
Cuts at: 0 319 1050
Size: 319 731
BlpI GC'TnA_GC
Cuts at: 0 90 265 1050
Size: 90 175 785
Fragments arranged by size:
785 175 90
Bme1580I G_kGCm'C
Cuts at: 0 544 556 811 928 1050
Size: 544 12 255 117 122
Fragments arranged by size:
544 255 122 117 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 421 940 1050
Size: 300 121 519 110
Fragments arranged by size:
519 300 121 110
Bpu10I CC'TnA_GC
Cuts at: 0 380 1050
Size: 380 670
BsaBI GATnn'nnATC
Cuts at: 0 415 1050
Size: 415 635
BsaHI Gr'CG_yC
Cuts at: 0 16 29 517 1050
Size: 16 13 488 533
Fragments arranged by size:
533 488 16 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 817 1050
Size: 235 582 233
Fragments arranged by size:
582 235 233
BseYI C'CCAG_C
Cuts at: 0 298 585 663 1050
Size: 298 287 78 387
Fragments arranged by size:
387 298 287 78
Bsp1286I G_dGCh'C
Cuts at: 0 544 556 811 928 1050
Size: 544 12 255 117 122
Fragments arranged by size:
544 255 122 117 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 264 439 577 639 1050
Size: 264 175 138 62 411
Fragments arranged by size:
411 264 175 138 62
BsrDI GCAATG_nn'
Cuts at: 0 684 1050
Size: 684 366
BsrFI r'CCGG_y
Cuts at: 0 18 269 1050
Size: 18 251 781
Fragments arranged by size:
781 251 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 202 211 1050
Size: 202 9 839
Fragments arranged by size:
839 202 9
BstBI TT'CG_AA
Cuts at: 0 65 1050
Size: 65 985
BstEII G'GTnAC_C
Cuts at: 0 1032 1050
Size: 1032 18
BstYI r'GATC_y
Cuts at: 0 905 1050
Size: 905 145
Bsu36I CC'TnA_GG
Cuts at: 0 126 1050
Size: 126 924
BtgI C'CryG_G
Cuts at: 0 504 803 1050
Size: 504 299 247
Fragments arranged by size:
504 299 247
BtsI GCAGTG_nn'
Cuts at: 0 658 769 791 1050
Size: 658 111 22 259
Fragments arranged by size:
658 259 111 22
DraIII CAC_nnn'GTG
Cuts at: 0 894 1050
Size: 894 156
DrdI GACnn_nn'nnGTC
Cuts at: 0 1041 1050
Size: 1041 9
EarI CTCTTCn'nnn_
Cuts at: 0 212 736 745 865 1050
Size: 212 524 9 120 185
Fragments arranged by size:
524 212 185 120 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 98 1050
Size: 98 952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 300 309 421 940 1050
Size: 236 64 9 112 519 110
Fragments arranged by size:
519 236 112 110 64 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 1050
Size: 172 878
EcoO109I rG'GnC_Cy
Cuts at: 0 320 808 924 925 951 1050
Size: 320 488 116 1 26 99
Fragments arranged by size:
488 320 116 99 26 1
FspI TGC'GCA
Cuts at: 0 775 1050
Size: 775 275
HaeII r_GCGC'y
Cuts at: 0 19 32 168 1050
Size: 19 13 136 882
Fragments arranged by size:
882 136 19 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 1050
Size: 109 32 909
Fragments arranged by size:
909 109 32
HincII GTy'rAC
Cuts at: 0 429 1050
Size: 429 621
KasI G'GCGC_C
Cuts at: 0 15 28 1050
Size: 15 13 1022
Fragments arranged by size:
1022 15 13
MslI CAynn'nnrTG
Cuts at: 0 547 1050
Size: 547 503
MspA1I CmG'CkG
Cuts at: 0 13 94 199 208 264 819 1050
Size: 13 81 105 9 56 555 231
Fragments arranged by size:
555 231 105 81 56 13 9
NaeI GCC'GGC
Cuts at: 0 20 271 1050
Size: 20 251 779
Fragments arranged by size:
779 251 20
NarI GG'CG_CC
Cuts at: 0 16 29 1050
Size: 16 13 1021
Fragments arranged by size:
1021 16 13
NcoI C'CATG_G
Cuts at: 0 504 1050
Size: 504 546
NgoMIV G'CCGG_C
Cuts at: 0 18 269 1050
Size: 18 251 781
Fragments arranged by size:
781 251 18
NspI r_CATG'y
Cuts at: 0 858 1050
Size: 858 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 894 1050
Size: 894 156
PfoI T'CCnGG_A
Cuts at: 0 398 533 908 1050
Size: 398 135 375 142
Fragments arranged by size:
398 375 142 135
PpuMI rG'GwC_Cy
Cuts at: 0 951 1050
Size: 951 99
PspOMI G'GGCC_C
Cuts at: 0 807 924 1050
Size: 807 117 126
Fragments arranged by size:
807 126 117
PstI C_TGCA'G
Cuts at: 0 650 1043 1050
Size: 650 393 7
Fragments arranged by size:
650 393 7
PvuII CAG'CTG
Cuts at: 0 94 199 208 264 1050
Size: 94 105 9 56 786
Fragments arranged by size:
786 105 94 56 9
RsrII CG'GwC_CG
Cuts at: 0 800 1050
Size: 800 250
SanDI GG'GwC_CC
Cuts at: 0 951 1050
Size: 951 99
SapI GCTCTTCn'nnn_
Cuts at: 0 865 1050
Size: 865 185
SfcI C'TryA_G
Cuts at: 0 8 646 1039 1050
Size: 8 638 393 11
Fragments arranged by size:
638 393 11 8
SfoI GGC'GCC
Cuts at: 0 17 30 1050
Size: 17 13 1020
Fragments arranged by size:
1020 17 13
SgrAI Cr'CCGG_yG
Cuts at: 0 18 1050
Size: 18 1032
StyI C'CwwG_G
Cuts at: 0 504 1050
Size: 504 546
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 139 745 1050
Size: 139 606 305
Fragments arranged by size:
606 305 139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 774 1050
Size: 774 276
ZraI GAC'GTC
Cuts at: 0 518 1050
Size: 518 532
Enzymes that do cut:
AarI AatII AccI AcuI AloI AlwNI ApaI BamHI
BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I
BpmI Bpu10I BsaBI BsaHI BseRI BseYI Bsp1286I BspMI
BsrDI BsrFI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtsI DraIII DrdI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HincII KasI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PflMI PfoI PpuMI PspOMI
PstI PvuII RsrII SanDI SapI SfcI SfoI SgrAI
StyI TaqII XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbvCI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaWI BsaXI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstXI BstZ17I BtgZI ClaI CspCI
DraI EaeI EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
SacI SacII SalI SbfI ScaI SexAI SfiI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI