(Circular) (Six-base) MAPSORT of: pFN21AB8617.seq Check: 560 from: 1 to: 6562 pFN21AB8617 6562 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4372 278 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2505 3905 1937 Size: 568 1400 4594 Fragments arranged by size: 4594 1400 568 Acc65I G'GTAC_C Cuts at: 3883 3883 Size: 6562 AclI AA'CG_TT Cuts at: 1563 4689 5062 1563 Size: 3126 373 3063 Fragments arranged by size: 3126 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2286 2519 4625 6009 6277 1632 Size: 452 202 233 2106 1384 268 1917 Fragments arranged by size: 2106 1917 1384 452 268 233 202 AfeI AGC'GCT Cuts at: 2300 2300 Size: 6562 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6365 AflIII A'CryG_T Cuts at: 1203 2968 5461 6438 1203 Size: 1765 2493 977 1327 Fragments arranged by size: 2493 1765 1327 977 AgeI A'CCGG_T Cuts at: 5393 5393 Size: 6562 AhdI GACnn_n'nnGTC Cuts at: 5291 5291 Size: 6562 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6562 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6562 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2565 2597 2565 Size: 32 6530 AlwNI CAG_nnn'CTG Cuts at: 2569 2750 3199 4305 5877 2569 Size: 181 449 1106 1572 3254 Fragments arranged by size: 3254 1572 1106 449 181 ApaLI G'TGCA_C Cuts at: 4619 5775 4619 Size: 1156 5406 ApoI r'AATT_y Cuts at: 1493 2574 3309 3733 3871 4097 4249 6358 1493 Size: 1081 735 424 138 226 152 2109 1697 Fragments arranged by size: 2109 1697 1081 735 424 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 5116 160 Size: 872 4084 1606 Fragments arranged by size: 4084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 AvaI C'yCGr_G Cuts at: 1870 1943 2234 3887 1870 Size: 73 291 1653 4545 Fragments arranged by size: 4545 1653 291 73 BamHI G'GATC_C Cuts at: 3707 3892 4259 3707 Size: 185 367 6010 Fragments arranged by size: 6010 367 185 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2677 3883 5338 618 Size: 325 217 627 890 1206 1455 1842 Fragments arranged by size: 1842 1455 1206 890 627 325 217 BanII G_rGCy'C Cuts at: 1391 2274 3026 3881 1391 Size: 883 752 855 4072 Fragments arranged by size: 4072 883 855 752 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2100 2547 3285 928 Size: 971 201 447 738 4205 Fragments arranged by size: 4205 971 738 447 201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2225 2259 4753 4787 1903 Size: 34 288 34 2494 34 3678 Fragments arranged by size: 3678 2494 288 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4454 5664 4454 Size: 1210 5352 BclI T'GATC_A Cuts at: 1548 3537 1548 Size: 1989 4573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2202 5173 136 Size: 107 122 71 631 605 530 2971 1525 Fragments arranged by size: 2971 1525 631 605 530 122 107 71 BglII A'GATC_T Cuts at: 2018 3376 6557 2018 Size: 1358 3181 2023 Fragments arranged by size: 3181 2023 1358 BlpI GC'TnA_GC Cuts at: 3979 3979 Size: 6562 Bme1580I G_kGCm'C Cuts at: 1790 4623 5779 1790 Size: 2833 1156 2573 Fragments arranged by size: 2833 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6562 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4293 5251 442 Size: 416 53 83 399 24 2876 958 1753 Fragments arranged by size: 2876 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3188 1056 Size: 2132 4430 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2832 2832 Size: 6562 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3246 3324 5222 1239 Size: 2007 78 1898 2579 Fragments arranged by size: 2579 2007 1898 78 Bpu10I CC'TnA_GC Cuts at: 2755 2755 Size: 6562 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4687 5552 5850 4687 Size: 865 298 5399 Fragments arranged by size: 5399 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5225 882 Size: 617 3726 2219 Fragments arranged by size: 3726 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4353 493 Size: 711 3149 2702 Fragments arranged by size: 3149 2702 711 BsaBI GATnn'nnATC Cuts at: 6556 6556 Size: 6562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4369 4751 5386 275 Size: 53 83 186 936 2836 382 635 1451 Fragments arranged by size: 2836 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4995 5393 5667 5814 6225 4995 Size: 398 274 147 411 5332 Fragments arranged by size: 5332 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6337 Fragments arranged by size: 6337 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6562 BseYI C'CCAG_C Cuts at: 2192 2301 2569 3283 5765 6261 2192 Size: 109 268 714 2482 496 2493 Fragments arranged by size: 2493 2482 714 496 268 109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2763 1163 Size: 573 1027 4962 Fragments arranged by size: 4962 1027 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4012 4773 5801 1540 Size: 275 186 2011 761 1028 2301 Fragments arranged by size: 2301 2011 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2517 3026 3509 4623 4708 5779 2517 Size: 509 483 1114 85 1071 3300 Fragments arranged by size: 3300 1114 1071 509 483 85 BsmI GAATG_Cn' Cuts at: 2044 2497 4073 4166 2044 Size: 453 1576 93 4440 Fragments arranged by size: 4440 1576 453 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2274 2517 3026 3509 3881 4623 4708 Size: 399 484 243 509 483 372 742 85 Cuts at: 4708 5779 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 509 484 483 399 372 243 85 BspEI T'CCGG_A Cuts at: 6225 6225 Size: 6562 BspHI T'CATG_A Cuts at: 3748 4451 6181 3748 Size: 703 1730 4129 Fragments arranged by size: 4129 1730 703 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3917 844 Size: 3073 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4449 1394 Size: 429 2626 3507 Fragments arranged by size: 3507 2626 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5057 5231 65 Size: 1183 3809 174 1396 Fragments arranged by size: 3809 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5206 5393 1700 Size: 110 3396 187 2869 Fragments arranged by size: 3396 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6562 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6562 BssSI C'ACGA_G Cuts at: 4622 5634 4622 Size: 1012 5550 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6562 BstBI TT'CG_AA Cuts at: 5366 5366 Size: 6562 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6562 BstXI CCAn_nnnn'nTGG Cuts at: 3602 3602 Size: 6562 BstYI r'GATC_y Cuts at: 1972 2018 3376 3707 3892 4259 4644 4661 6113 Size: 46 1358 331 185 367 385 17 1452 Cuts at: 6113 6199 6211 6557 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1358 385 367 346 331 185 86 46 17 12 Bsu36I CC'TnA_GG Cuts at: 2637 2637 Size: 6562 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6010 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5823 Fragments arranged by size: 5823 683 56 BtsI GCAGTG_nn' Cuts at: 4078 4872 4892 4078 Size: 794 20 5748 Fragments arranged by size: 5748 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6527 DraI TTT'AAA Cuts at: 3315 3866 3315 Size: 551 6011 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5569 809 Size: 507 4253 1802 Fragments arranged by size: 4253 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2203 2736 4009 Size: 54 1425 50 275 18 373 533 1273 Cuts at: 4009 4898 6527 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1273 889 533 373 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4009 1537 Size: 275 2197 4090 Fragments arranged by size: 4090 2197 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 2427 2719 3601 3677 3752 4492 1353 Size: 909 165 292 882 76 75 740 3423 Fragments arranged by size: 3423 909 882 740 292 165 76 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5149 5523 5669 387 Size: 1064 138 3560 374 146 1280 Fragments arranged by size: 3560 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3024 3024 Size: 6562 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2286 2519 3246 3324 4625 5222 Size: 393 452 202 233 727 78 1301 597 Cuts at: 5222 6009 6277 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1301 787 727 597 452 393 268 233 202 78 EcoRI G'AATT_C Cuts at: 3871 4249 3871 Size: 378 6184 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3346 3346 Size: 6562 FspI TGC'GCA Cuts at: 5068 5068 Size: 6562 HaeII r_GCGC'y Cuts at: 1678 2302 5709 1678 Size: 624 3407 2531 Fragments arranged by size: 3407 2531 624 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2340 2372 2465 2497 2539 Size: 32 135 32 768 32 93 32 42 Cuts at: 2539 2571 2738 2770 5203 5235 5277 5309 1373 Size: 32 167 32 2433 32 42 32 2626 Fragments arranged by size: 2626 2433 768 167 135 93 42 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3906 4152 669 Size: 988 281 1968 246 3079 Fragments arranged by size: 3079 1968 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6562 HpaI GTT'AAC Cuts at: 4152 4152 Size: 6562 KpnI G_GTAC'C Cuts at: 3887 3887 Size: 6562 MfeI C'AATT_G Cuts at: 4161 4161 Size: 6562 MluI A'CGCG_T Cuts at: 6438 6438 Size: 6562 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 3191 5676 5860 1305 Size: 780 1106 2485 184 2007 Fragments arranged by size: 2485 2007 1106 780 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2205 2738 10 Size: 54 1425 343 373 533 3834 Fragments arranged by size: 3834 1425 533 373 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2693 4520 4879 5038 518 Size: 648 1527 1827 359 159 2042 Fragments arranged by size: 2042 1827 1527 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2569 2768 3978 4655 5803 6048 1606 Size: 963 199 1210 677 1148 245 2120 Fragments arranged by size: 2120 1210 1148 963 677 245 199 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6010 NdeI CA'TA_TG Cuts at: 387 387 Size: 6562 NheI G'CTAG_C Cuts at: 1052 3184 1052 Size: 2132 4430 NotI GC'GGCC_GC Cuts at: 4009 4009 Size: 6562 NspI r_CATG'y Cuts at: 1132 1229 2972 3920 5465 1132 Size: 97 1743 948 1545 2229 Fragments arranged by size: 2229 1743 1545 948 97 PasI CC'CwG_GG Cuts at: 1348 2993 1348 Size: 1645 4917 PciI A'CATG_T Cuts at: 2968 5461 2968 Size: 2493 4069 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6562 PmeI GTTT'AAAC Cuts at: 3866 3866 Size: 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4631 4663 6144 6176 4631 Size: 32 1481 32 5017 Fragments arranged by size: 5017 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6562 PsiI TTA'TAA Cuts at: 4132 4132 Size: 6562 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3625 3657 3625 Size: 32 6530 PstI C_TGCA'G Cuts at: 830 3914 830 Size: 3084 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6562 PvuII CAG'CTG Cuts at: 2569 2768 2569 Size: 199 6363 SacI G_AGCT'C Cuts at: 3026 3026 Size: 6562 SalI G'TCGA_C Cuts at: 1936 3904 1936 Size: 1968 4594 SapI GCTCTTCn'nnn_ Cuts at: 2262 2262 Size: 6562 SbfI CC_TGCA'GG Cuts at: 3914 3914 Size: 6562 ScaI AGT'ACT Cuts at: 4810 6522 4810 Size: 1712 4850 SfcI C'TryA_G Cuts at: 826 1045 2136 2142 3027 3910 5045 5726 5917 Size: 219 1091 6 885 883 1135 681 191 Cuts at: 5917 826 Size: 1471 Fragments arranged by size: 1471 1135 1091 885 883 681 219 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6562 SmaI CCC'GGG Cuts at: 1872 3889 1872 Size: 2017 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4666 5567 5829 820 Size: 197 926 2723 901 262 1553 Fragments arranged by size: 2723 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4353 493 Size: 3860 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6562 SphI G_CATG'C Cuts at: 1132 3920 1132 Size: 2788 3774 SspI AAT'ATT Cuts at: 5 52 4486 5 Size: 47 4434 2081 Fragments arranged by size: 4434 2081 47 StuI AGG'CCT Cuts at: 1517 3528 1517 Size: 2011 4551 StyI C'CwwG_G Cuts at: 513 1065 4001 513 Size: 552 2936 3074 Fragments arranged by size: 3074 2936 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4589 4606 4759 4944 5389 325 Size: 1255 3009 17 153 185 445 1498 Fragments arranged by size: 3009 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4808 6520 96 Size: 274 80 33 51 1443 2831 1712 138 Fragments arranged by size: 2831 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6522 6554 6522 Size: 32 6530 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6562 XbaI T'CTAG_A Cuts at: 3898 3898 Size: 6562 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3082 3082 Size: 6562 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6562 XmaI C'CCGG_G Cuts at: 1870 3887 1870 Size: 2017 4545 XmnI GAAnn'nnTTC Cuts at: 2097 3123 3393 4691 2097 Size: 1026 270 1298 3968 Fragments arranged by size: 3968 1298 1026 270 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4370 276 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI