Restriction map for pFN21AB8617
 (Circular) (Six-base) MAPSORT of: pFN21AB8617.seq  Check: 560  from: 1  to: 6562



pFN21AB8617  6562 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:27  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4372     278 
   Size:         53      83     186     936    2836    2468

  Fragments arranged by size: 

                2836    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2505    3905    1937 
   Size:        568    1400    4594

  Fragments arranged by size: 

                4594    1400     568
 
Acc65I G'GTAC_C

Cuts at:   3883    3883 
   Size:       6562
 
AclI AA'CG_TT

Cuts at:   1563    4689    5062    1563 
   Size:       3126     373    3063

  Fragments arranged by size: 

                3126    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2084    2286    2519    4625    6009    6277    1632 
   Size:        452     202     233    2106    1384     268    1917

  Fragments arranged by size: 

                2106    1917    1384     452     268     233     202
 
AfeI AGC'GCT

Cuts at:   2300    2300 
   Size:       6562
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6365
 
AflIII A'CryG_T

Cuts at:   1203    2968    5461    6438    1203 
   Size:       1765    2493     977    1327

  Fragments arranged by size: 

                2493    1765    1327     977
 
AgeI A'CCGG_T

Cuts at:   5393    5393 
   Size:       6562
 
AhdI GACnn_n'nnGTC

Cuts at:   5291    5291 
   Size:       6562
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6562
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6562
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2565    2597    2565 
   Size:         32    6530
 
AlwNI CAG_nnn'CTG

Cuts at:   2569    2750    3199    4305    5877    2569 
   Size:        181     449    1106    1572    3254

  Fragments arranged by size: 

                3254    1572    1106     449     181
 
ApaLI G'TGCA_C

Cuts at:   4619    5775    4619 
   Size:       1156    5406
 
ApoI r'AATT_y

Cuts at:   1493    2574    3309    3733    3871    4097    4249    6358    1493 
   Size:       1081     735     424     138     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1081     735     424     226     152     138
            
 
AseI AT'TA_AT

Cuts at:    160    1032    5116     160 
   Size:        872    4084    1606

  Fragments arranged by size: 

                4084    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6562
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2234    3887    1870 
   Size:         73     291    1653    4545

  Fragments arranged by size: 

                4545    1653     291      73
 
BamHI G'GATC_C

Cuts at:   3707    3892    4259    3707 
   Size:        185     367    6010

  Fragments arranged by size: 

                6010     367     185
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2677    3883    5338     618 
   Size:        325     217     627     890    1206    1455    1842

  Fragments arranged by size: 

                1842    1455    1206     890     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2274    3026    3881    1391 
   Size:        883     752     855    4072

  Fragments arranged by size: 

                4072     883     855     752
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2100    2547    3285     928 
   Size:        971     201     447     738    4205

  Fragments arranged by size: 

                4205     971     738     447     201
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2225    2259    4753    4787    1903 
   Size:         34     288      34    2494      34    3678

  Fragments arranged by size: 

                3678    2494     288      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4454    5664    4454 
   Size:       1210    5352
 
BclI T'GATC_A

Cuts at:   1548    3537    1548 
   Size:       1989    4573
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2202    5173     136 
   Size:        107     122      71     631     605     530    2971    1525

  Fragments arranged by size: 

                2971    1525     631     605     530     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2018    3376    6557    2018 
   Size:       1358    3181    2023

  Fragments arranged by size: 

                3181    2023    1358
 
BlpI GC'TnA_GC

Cuts at:   3979    3979 
   Size:       6562
 
Bme1580I G_kGCm'C

Cuts at:   1790    4623    5779    1790 
   Size:       2833    1156    2573

  Fragments arranged by size: 

                2833    2573    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6562
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4293    5251     442 
   Size:        416      53      83     399      24    2876     958    1753

  Fragments arranged by size: 

                2876    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    3188    1056 
   Size:       2132    4430
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2832    2832 
   Size:       6562
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3246    3324    5222    1239 
   Size:       2007      78    1898    2579

  Fragments arranged by size: 

                2579    2007    1898      78
 
Bpu10I CC'TnA_GC

Cuts at:   2755    2755 
   Size:       6562
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4687    5552    5850    4687 
   Size:        865     298    5399

  Fragments arranged by size: 

                5399     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5225     882 
   Size:        617    3726    2219

  Fragments arranged by size: 

                3726    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4353     493 
   Size:        711    3149    2702

  Fragments arranged by size: 

                3149    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6556    6556 
   Size:       6562
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4369    4751    5386     275 
   Size:         53      83     186     936    2836     382     635    1451

  Fragments arranged by size: 

                2836    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4995    5393    5667    5814    6225    4995 
   Size:        398     274     147     411    5332

  Fragments arranged by size: 

                5332     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6337

  Fragments arranged by size: 

                6337     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       6562
 
BseYI C'CCAG_C

Cuts at:   2192    2301    2569    3283    5765    6261    2192 
   Size:        109     268     714    2482     496    2493

  Fragments arranged by size: 

                2493    2482     714     496     268     109
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2763    1163 
   Size:        573    1027    4962

  Fragments arranged by size: 

                4962    1027     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4012    4773    5801    1540 
   Size:        275     186    2011     761    1028    2301

  Fragments arranged by size: 

                2301    2011    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2517    3026    3509    4623    4708    5779    2517 
   Size:        509     483    1114      85    1071    3300

  Fragments arranged by size: 

                3300    1114    1071     509     483      85
 
BsmI GAATG_Cn'

Cuts at:   2044    2497    4073    4166    2044 
   Size:        453    1576      93    4440

  Fragments arranged by size: 

                4440    1576     453      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2274    2517    3026    3509    3881    4623    4708 
   Size:        399     484     243     509     483     372     742      85

Cuts at:   4708    5779    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     509     484     483     399     372
                 243      85
 
BspEI T'CCGG_A

Cuts at:   6225    6225 
   Size:       6562
 
BspHI T'CATG_A

Cuts at:   3748    4451    6181    3748 
   Size:        703    1730    4129

  Fragments arranged by size: 

                4129    1730     703
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3917     844 
   Size:       3073    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4449    1394 
   Size:        429    2626    3507

  Fragments arranged by size: 

                3507    2626     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    5057    5231      65 
   Size:       1183    3809     174    1396

  Fragments arranged by size: 

                3809    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5206    5393    1700 
   Size:        110    3396     187    2869

  Fragments arranged by size: 

                3396    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6562
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6562
 
BssSI C'ACGA_G

Cuts at:   4622    5634    4622 
   Size:       1012    5550
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6562
 
BstBI TT'CG_AA

Cuts at:   5366    5366 
   Size:       6562
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6562
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3602    3602 
   Size:       6562
 
BstYI r'GATC_y

Cuts at:   1972    2018    3376    3707    3892    4259    4644    4661    6113 
   Size:         46    1358     331     185     367     385      17    1452

Cuts at:   6113    6199    6211    6557    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1358     385     367     346     331     185
                  86      46      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2637    2637 
   Size:       6562
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    6010
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5823

  Fragments arranged by size: 

                5823     683      56
 
BtsI GCAGTG_nn'

Cuts at:   4078    4872    4892    4078 
   Size:        794      20    5748

  Fragments arranged by size: 

                5748     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6562
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6527
 
DraI TTT'AAA

Cuts at:   3315    3866    3315 
   Size:        551    6011
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5569     809 
   Size:        507    4253    1802

  Fragments arranged by size: 

                4253    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2203    2736    4009 
   Size:         54    1425      50     275      18     373     533    1273

Cuts at:   4009    4898    6527       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425    1273     889     533     373     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4009    1537 
   Size:        275    2197    4090

  Fragments arranged by size: 

                4090    2197     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2262    2427    2719    3601    3677    3752    4492    1353 
   Size:        909     165     292     882      76      75     740    3423

  Fragments arranged by size: 

                3423     909     882     740     292     165      76      75
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5149    5523    5669     387 
   Size:       1064     138    3560     374     146    1280

  Fragments arranged by size: 

                3560    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   3024    3024 
   Size:       6562
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2084    2286    2519    3246    3324    4625    5222 
   Size:        393     452     202     233     727      78    1301     597

Cuts at:   5222    6009    6277    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524    1301     787     727     597     452     393     268
                 233     202      78
 
EcoRI G'AATT_C

Cuts at:   3871    4249    3871 
   Size:        378    6184
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3346    3346 
   Size:       6562
 
FspI TGC'GCA

Cuts at:   5068    5068 
   Size:       6562
 
HaeII r_GCGC'y

Cuts at:   1678    2302    5709    1678 
   Size:        624    3407    2531

  Fragments arranged by size: 

                3407    2531     624
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2340    2372    2465    2497    2539 
   Size:         32     135      32     768      32      93      32      42

Cuts at:   2539    2571    2738    2770    5203    5235    5277    5309    1373 
   Size:         32     167      32    2433      32      42      32    2626

  Fragments arranged by size: 

                2626    2433     768     167     135      93      42      42
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3906    4152     669 
   Size:        988     281    1968     246    3079

  Fragments arranged by size: 

                3079    1968     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6562
 
HpaI GTT'AAC

Cuts at:   4152    4152 
   Size:       6562
 
KpnI G_GTAC'C

Cuts at:   3887    3887 
   Size:       6562
 
MfeI C'AATT_G

Cuts at:   4161    4161 
   Size:       6562
 
MluI A'CGCG_T

Cuts at:   6438    6438 
   Size:       6562
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2085    3191    5676    5860    1305 
   Size:        780    1106    2485     184    2007

  Fragments arranged by size: 

                2485    2007    1106     780     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2205    2738      10 
   Size:         54    1425     343     373     533    3834

  Fragments arranged by size: 

                3834    1425     533     373     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2693    4520    4879    5038     518 
   Size:        648    1527    1827     359     159    2042

  Fragments arranged by size: 

                2042    1827    1527     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2569    2768    3978    4655    5803    6048    1606 
   Size:        963     199    1210     677    1148     245    2120

  Fragments arranged by size: 

                2120    1210    1148     963     677     245     199
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    6010
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6562
 
NheI G'CTAG_C

Cuts at:   1052    3184    1052 
   Size:       2132    4430
 
NotI GC'GGCC_GC

Cuts at:   4009    4009 
   Size:       6562
 
NspI r_CATG'y

Cuts at:   1132    1229    2972    3920    5465    1132 
   Size:         97    1743     948    1545    2229

  Fragments arranged by size: 

                2229    1743    1545     948      97
 
PasI CC'CwG_GG

Cuts at:   1348    2993    1348 
   Size:       1645    4917
 
PciI A'CATG_T

Cuts at:   2968    5461    2968 
   Size:       2493    4069
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6562
 
PmeI GTTT'AAAC

Cuts at:   3866    3866 
   Size:       6562
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4631    4663    6144    6176    4631 
   Size:         32    1481      32    5017

  Fragments arranged by size: 

                5017    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6562
 
PsiI TTA'TAA

Cuts at:   4132    4132 
   Size:       6562
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   3625    3657    3625 
   Size:         32    6530
 
PstI C_TGCA'G

Cuts at:    830    3914     830 
   Size:       3084    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6562
 
PvuII CAG'CTG

Cuts at:   2569    2768    2569 
   Size:        199    6363
 
SacI G_AGCT'C

Cuts at:   3026    3026 
   Size:       6562
 
SalI G'TCGA_C

Cuts at:   1936    3904    1936 
   Size:       1968    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2262    2262 
   Size:       6562
 
SbfI CC_TGCA'GG

Cuts at:   3914    3914 
   Size:       6562
 
ScaI AGT'ACT

Cuts at:   4810    6522    4810 
   Size:       1712    4850
 
SfcI C'TryA_G

Cuts at:    826    1045    2136    2142    3027    3910    5045    5726    5917 
   Size:        219    1091       6     885     883    1135     681     191

Cuts at:   5917     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135    1091     885     883     681     219     191
                   6
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6562
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6562
 
SmaI CCC'GGG

Cuts at:   1872    3889    1872 
   Size:       2017    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4666    5567    5829     820 
   Size:        197     926    2723     901     262    1553

  Fragments arranged by size: 

                2723    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4353     493 
   Size:       3860    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6562
 
SphI G_CATG'C

Cuts at:   1132    3920    1132 
   Size:       2788    3774
 
SspI AAT'ATT

Cuts at:      5      52    4486       5 
   Size:         47    4434    2081

  Fragments arranged by size: 

                4434    2081      47
 
StuI AGG'CCT

Cuts at:   1517    3528    1517 
   Size:       2011    4551
 
StyI C'CwwG_G

Cuts at:    513    1065    4001     513 
   Size:        552    2936    3074

  Fragments arranged by size: 

                3074    2936     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4589    4606    4759    4944    5389     325 
   Size:       1255    3009      17     153     185     445    1498

  Fragments arranged by size: 

                3009    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4808    6520      96 
   Size:        274      80      33      51    1443    2831    1712     138

  Fragments arranged by size: 

                2831    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6522    6554    6522 
   Size:         32    6530
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6562
 
XbaI T'CTAG_A

Cuts at:   3898    3898 
   Size:       6562
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3082    3082 
   Size:       6562
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6562
 
XmaI C'CCGG_G

Cuts at:   1870    3887    1870 
   Size:       2017    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2097    3123    3393    4691    2097 
   Size:       1026     270    1298    3968

  Fragments arranged by size: 

                3968    1298    1026     270
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4370     276 
   Size:         53      83     186     936    2836    2468

  Fragments arranged by size: 

                2836    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PsrI     PstI     PvuI    PvuII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
    BsiWI    BsmBI  BstZ17I   DraIII    EcoNI EcoO109I    EcoRV     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI    RsrII    SacII    SanDI
    SexAI     SfiI     SfoI     SrfI     SwaI
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