(Circular) (Six-base) MAPSORT of: pFN21AB8592.seq Check: 8685 from: 1 to: 7072 pFN21AB8592 7072 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4306 4306 Size: 7072 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4882 278 Size: 53 83 186 936 3346 2468 Fragments arranged by size: 3346 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4415 1937 Size: 2478 4594 Acc65I G'GTAC_C Cuts at: 4393 4393 Size: 7072 AclI AA'CG_TT Cuts at: 1563 5199 5572 1563 Size: 3636 373 3063 Fragments arranged by size: 3636 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2646 3314 3459 3768 5135 6519 6787 1632 Size: 1014 668 145 309 1367 1384 268 1917 Fragments arranged by size: 1917 1384 1367 1014 668 309 268 145 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6875 AflIII A'CryG_T Cuts at: 1203 2196 2421 4249 5971 6948 1203 Size: 993 225 1828 1722 977 1327 Fragments arranged by size: 1828 1722 1327 993 977 225 AgeI A'CCGG_T Cuts at: 5903 5903 Size: 7072 AhdI GACnn_n'nnGTC Cuts at: 5801 5801 Size: 7072 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7072 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7072 AlwNI CAG_nnn'CTG Cuts at: 4815 6387 4815 Size: 1572 5500 ApaLI G'TGCA_C Cuts at: 5129 6285 5129 Size: 1156 5916 ApoI r'AATT_y Cuts at: 1493 2297 2334 2413 2920 3359 3820 4238 4363 Size: 804 37 79 507 439 461 418 125 Cuts at: 4363 4381 4607 4759 6868 1493 Size: 18 226 152 2109 1697 Fragments arranged by size: 2109 1697 804 507 461 439 418 226 152 125 79 37 18 AseI AT'TA_AT Cuts at: 160 1032 5626 160 Size: 872 4594 1606 Fragments arranged by size: 4594 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7072 AvaI C'yCGr_G Cuts at: 1870 1943 4397 1870 Size: 73 2454 4545 Fragments arranged by size: 4545 2454 73 BamHI G'GATC_C Cuts at: 4402 4769 4402 Size: 367 6705 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4393 5848 618 Size: 325 217 627 2606 1455 1842 Fragments arranged by size: 2606 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4391 1391 Size: 3000 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2831 3320 3424 4020 928 Size: 971 932 489 104 596 3980 Fragments arranged by size: 3980 971 932 596 489 104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5263 5297 1903 Size: 34 3326 34 3678 Fragments arranged by size: 3678 3326 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4964 6174 4964 Size: 1210 5862 BclI T'GATC_A Cuts at: 1548 4228 4348 1548 Size: 2680 120 4272 Fragments arranged by size: 4272 2680 120 BfrBI ATG'CAT Cuts at: 3923 3923 Size: 7072 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5683 136 Size: 107 122 71 631 605 4011 1525 Fragments arranged by size: 4011 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3313 7067 3313 Size: 3754 3318 BlpI GC'TnA_GC Cuts at: 4489 4489 Size: 7072 Bme1580I G_kGCm'C Cuts at: 1790 4277 5133 6289 1790 Size: 2487 856 1156 2573 Fragments arranged by size: 2573 2487 1156 856 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7072 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4803 5761 442 Size: 416 53 83 399 24 3386 958 1753 Fragments arranged by size: 3386 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7072 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2199 2199 Size: 7072 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2361 5732 1239 Size: 1122 3371 2579 Fragments arranged by size: 3371 2579 1122 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2474 2726 2765 3019 5197 6062 6360 2474 Size: 252 39 254 2178 865 298 3186 Fragments arranged by size: 3186 2178 865 298 254 252 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5735 882 Size: 617 4236 2219 Fragments arranged by size: 4236 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4139 4863 493 Size: 711 2935 724 2702 Fragments arranged by size: 2935 2702 724 711 BsaBI GATnn'nnATC Cuts at: 7066 7066 Size: 7072 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4879 5261 5896 275 Size: 53 83 186 936 3346 382 635 1451 Fragments arranged by size: 3346 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5505 5903 6177 6324 6735 5505 Size: 398 274 147 411 5842 Fragments arranged by size: 5842 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2104 2134 1178 Size: 30 165 30 701 30 6116 Fragments arranged by size: 6116 701 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7072 BseYI C'CCAG_C Cuts at: 3490 6275 6771 3490 Size: 2785 496 3791 Fragments arranged by size: 3791 2785 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3168 4303 4355 1163 Size: 573 1432 1135 52 3880 Fragments arranged by size: 3880 1432 1135 573 52 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4522 5283 6311 1540 Size: 275 186 2521 761 1028 2301 Fragments arranged by size: 2521 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5133 5218 6289 5133 Size: 85 1071 5916 Fragments arranged by size: 5916 1071 85 BsiWI C'GTAC_G Cuts at: 3151 3151 Size: 7072 BsmI GAATG_Cn' Cuts at: 2261 3374 4583 4676 2261 Size: 1113 1209 93 4657 Fragments arranged by size: 4657 1209 1113 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4277 4391 5133 5218 6289 1391 Size: 399 2487 114 742 85 1071 2174 Fragments arranged by size: 2487 2174 1071 742 399 114 85 BspEI T'CCGG_A Cuts at: 6735 6735 Size: 7072 BspHI T'CATG_A Cuts at: 4961 6691 4961 Size: 1730 5342 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3183 4306 4427 844 Size: 2339 1123 121 3489 Fragments arranged by size: 3489 2339 1123 121 BsrBI CCG'CTC Cuts at: 1394 1823 4959 1394 Size: 429 3136 3507 Fragments arranged by size: 3507 3136 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2027 5567 5741 65 Size: 1183 779 3540 174 1396 Fragments arranged by size: 3540 1396 1183 779 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5716 5903 1700 Size: 110 3906 187 2869 Fragments arranged by size: 3906 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7072 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7072 BssSI C'ACGA_G Cuts at: 2184 5132 6144 2184 Size: 2948 1012 3112 Fragments arranged by size: 3112 2948 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3953 794 Size: 3159 3913 BstBI TT'CG_AA Cuts at: 5876 5876 Size: 7072 BstEII G'GTnAC_C Cuts at: 1184 2431 1184 Size: 1247 5825 BstXI CCAn_nnnn'nTGG Cuts at: 2148 2148 Size: 7072 BstYI r'GATC_y Cuts at: 1972 3313 4402 4769 5154 5171 6623 6709 6721 Size: 1341 1089 367 385 17 1452 86 12 Cuts at: 6721 7067 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1341 1089 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6520 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6333 Fragments arranged by size: 6333 683 56 BtsI GCAGTG_nn' Cuts at: 3234 4588 5382 5402 3234 Size: 1354 794 20 4904 Fragments arranged by size: 4904 1354 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7072 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7037 DraI TTT'AAA Cuts at: 2769 2916 3060 4376 2769 Size: 147 144 1316 5465 Fragments arranged by size: 5465 1316 147 144 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4022 6079 809 Size: 507 2706 2057 1802 Fragments arranged by size: 2706 2057 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 4519 5408 Size: 54 1425 50 275 18 240 2449 889 Cuts at: 5408 7037 8 Size: 1629 43 Fragments arranged by size: 2449 1629 1425 889 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4519 1537 Size: 275 2707 4090 Fragments arranged by size: 4090 2707 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 2652 2663 2958 3295 5002 1353 Size: 1026 273 11 295 337 1707 3423 Fragments arranged by size: 3423 1707 1026 337 295 273 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5659 6033 6179 387 Size: 1064 138 4070 374 146 1280 Fragments arranged by size: 4070 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2361 2646 3314 3459 3768 5135 5732 Size: 393 729 285 668 145 309 1367 597 Cuts at: 5732 6519 6787 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1367 787 729 668 597 393 309 285 268 145 EcoRI G'AATT_C Cuts at: 3820 4381 4759 3820 Size: 561 378 6133 Fragments arranged by size: 6133 561 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2370 2408 2465 2370 Size: 38 57 6977 Fragments arranged by size: 6977 57 38 FspI TGC'GCA Cuts at: 5578 5578 Size: 7072 HaeII r_GCGC'y Cuts at: 1678 6219 1678 Size: 4541 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2229 2261 2672 2704 2705 Size: 32 135 32 657 32 411 32 1 Cuts at: 2705 2737 5713 5745 5787 5819 1373 Size: 32 2976 32 42 32 2626 Fragments arranged by size: 2976 2626 657 411 135 42 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3148 4130 4416 4662 669 Size: 988 281 1210 982 286 246 3079 Fragments arranged by size: 3079 1210 988 982 286 281 246 HindIII A'AGCT_T Cuts at: 748 2638 2891 748 Size: 1890 253 4929 Fragments arranged by size: 4929 1890 253 HpaI GTT'AAC Cuts at: 4662 4662 Size: 7072 KpnI G_GTAC'C Cuts at: 4397 4397 Size: 7072 MfeI C'AATT_G Cuts at: 2248 2739 4671 2248 Size: 491 1932 4649 Fragments arranged by size: 4649 1932 491 MluI A'CGCG_T Cuts at: 6948 6948 Size: 7072 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2064 2149 6186 6370 1305 Size: 759 85 4037 184 2007 Fragments arranged by size: 4037 2007 759 184 85 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 10 Size: 54 1425 343 240 5010 Fragments arranged by size: 5010 1425 343 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 2426 4137 5030 5389 5548 518 Size: 648 1162 98 1711 893 359 159 2042 Fragments arranged by size: 2042 1711 1162 893 648 359 159 98 MspA1I CmG'CkG Cuts at: 1606 4488 5165 6313 6558 1606 Size: 2882 677 1148 245 2120 Fragments arranged by size: 2882 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6520 NdeI CA'TA_TG Cuts at: 387 387 Size: 7072 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7072 NotI GC'GGCC_GC Cuts at: 4519 4519 Size: 7072 NsiI A_TGCA'T Cuts at: 3925 3925 Size: 7072 NspI r_CATG'y Cuts at: 1132 1229 2425 3852 4253 4334 4430 5975 1132 Size: 97 1196 1427 401 81 96 1545 2229 Fragments arranged by size: 2229 1545 1427 1196 401 97 96 81 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7072 PciI A'CATG_T Cuts at: 2421 4249 5971 2421 Size: 1828 1722 3522 Fragments arranged by size: 3522 1828 1722 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7072 PmeI GTTT'AAAC Cuts at: 4376 4376 Size: 7072 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5141 5173 6654 6686 5141 Size: 32 1481 32 5527 Fragments arranged by size: 5527 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7072 PsiI TTA'TAA Cuts at: 2853 3351 3765 4642 2853 Size: 498 414 877 5283 Fragments arranged by size: 5283 877 498 414 PstI C_TGCA'G Cuts at: 830 2982 3343 3538 4031 4424 830 Size: 2152 361 195 493 393 3478 Fragments arranged by size: 3478 2152 493 393 361 195 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7072 SalI G'TCGA_C Cuts at: 1936 4414 1936 Size: 2478 4594 SapI GCTCTTCn'nnn_ Cuts at: 2379 2379 Size: 7072 SbfI CC_TGCA'GG Cuts at: 4424 4424 Size: 7072 ScaI AGT'ACT Cuts at: 2179 5320 7032 2179 Size: 3141 1712 2219 Fragments arranged by size: 3141 2219 1712 SfcI C'TryA_G Cuts at: 826 1045 2978 3339 3510 3534 4027 4420 5555 Size: 219 1933 361 171 24 493 393 1135 Cuts at: 5555 6236 6427 826 Size: 681 191 1471 Fragments arranged by size: 1933 1471 1135 681 493 393 361 219 191 171 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7072 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7072 SmaI CCC'GGG Cuts at: 1872 4399 1872 Size: 2527 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 2705 2780 3034 5176 6077 Size: 197 926 510 252 75 254 2142 901 Cuts at: 6077 6339 820 Size: 262 1553 Fragments arranged by size: 2142 1553 926 901 510 262 254 252 197 75 SnaBI TAC'GTA Cuts at: 493 4863 493 Size: 4370 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7072 SphI G_CATG'C Cuts at: 1132 4430 1132 Size: 3298 3774 SspI AAT'ATT Cuts at: 5 52 4996 5 Size: 47 4944 2081 Fragments arranged by size: 4944 2081 47 StuI AGG'CCT Cuts at: 1517 2448 1517 Size: 931 6141 StyI C'CwwG_G Cuts at: 513 1065 2266 4511 513 Size: 552 1201 2245 3074 Fragments arranged by size: 3074 2245 1201 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5099 5116 5269 5454 5899 325 Size: 1255 3519 17 153 185 445 1498 Fragments arranged by size: 3519 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2177 2254 2348 Size: 274 80 33 51 1443 200 77 94 Cuts at: 2348 3751 5318 7030 96 Size: 1403 1567 1712 138 Fragments arranged by size: 1712 1567 1443 1403 274 200 138 94 80 77 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2104 2136 7032 7064 2104 Size: 32 4896 32 2112 Fragments arranged by size: 4896 2112 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7072 XbaI T'CTAG_A Cuts at: 3784 4408 3784 Size: 624 6448 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7072 XmaI C'CCGG_G Cuts at: 1870 4397 1870 Size: 2527 4545 XmnI GAAnn'nnTTC Cuts at: 2672 3090 5201 2672 Size: 418 2111 4543 Fragments arranged by size: 4543 2111 418 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4880 276 Size: 53 83 186 936 3346 2468 Fragments arranged by size: 3346 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI