(Circular) (Six-base) MAPSORT of: pFN21AB8591.seq Check: 7366 from: 1 to: 7015 pFN21AB8591 7015 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4825 278 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4358 1937 Size: 2421 4594 Acc65I G'GTAC_C Cuts at: 4336 4336 Size: 7015 AclI AA'CG_TT Cuts at: 1563 5142 5515 1563 Size: 3579 373 3063 Fragments arranged by size: 3579 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3061 4188 5078 6462 6730 1632 Size: 1429 1127 890 1384 268 1917 Fragments arranged by size: 1917 1429 1384 1127 890 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6818 AflIII A'CryG_T Cuts at: 1203 4295 5914 6891 1203 Size: 3092 1619 977 1327 Fragments arranged by size: 3092 1619 1327 977 AgeI A'CCGG_T Cuts at: 5846 5846 Size: 7015 AhdI GACnn_n'nnGTC Cuts at: 3113 3664 5744 3113 Size: 551 2080 4384 Fragments arranged by size: 4384 2080 551 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7015 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2955 1184 Size: 1771 5244 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2122 2154 2122 Size: 32 6983 AlwNI CAG_nnn'CTG Cuts at: 2543 2903 4758 6330 2543 Size: 360 1855 1572 3228 Fragments arranged by size: 3228 1855 1572 360 ApaI G_GGCC'C Cuts at: 3973 3973 Size: 7015 ApaLI G'TGCA_C Cuts at: 5072 6228 5072 Size: 1156 5859 ApoI r'AATT_y Cuts at: 1493 4324 4550 4702 6811 1493 Size: 2831 226 152 2109 1697 Fragments arranged by size: 2831 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5569 160 Size: 872 4537 1606 Fragments arranged by size: 4537 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2039 2247 2769 2834 4340 1870 Size: 73 89 7 208 522 65 1506 4545 Fragments arranged by size: 4545 1506 522 208 89 73 65 7 BamHI G'GATC_C Cuts at: 2215 4345 4712 2215 Size: 2130 367 4518 Fragments arranged by size: 4518 2130 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2243 2426 3798 4336 Size: 325 217 627 335 121 183 1372 538 Cuts at: 4336 5791 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1372 627 538 335 325 217 183 121 BanII G_rGCy'C Cuts at: 1391 2708 3415 3973 4117 4285 4334 1391 Size: 1317 707 558 144 168 49 4072 Fragments arranged by size: 4072 1317 707 558 168 144 49 BbeI G_GCGC'C Cuts at: 2247 2247 Size: 7015 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2852 928 Size: 971 953 5091 Fragments arranged by size: 5091 971 953 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5206 5240 1903 Size: 34 50 34 3185 34 3678 Fragments arranged by size: 3678 3185 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2110 4907 6117 2110 Size: 2797 1210 3008 Fragments arranged by size: 3008 2797 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7015 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2665 5626 Size: 107 122 71 631 605 337 656 2961 Cuts at: 5626 136 Size: 1525 Fragments arranged by size: 2961 1525 656 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3433 7010 3433 Size: 3577 3438 BlpI GC'TnA_GC Cuts at: 2087 2529 3414 3419 3775 4432 2087 Size: 442 885 5 356 657 4670 Fragments arranged by size: 4670 885 657 442 356 5 Bme1580I G_kGCm'C Cuts at: 1790 2127 3135 3973 5076 6232 1790 Size: 337 1008 838 1103 1156 2573 Fragments arranged by size: 2573 1156 1103 1008 838 337 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7015 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2995 3581 3801 Size: 416 53 83 399 24 1578 586 220 Cuts at: 3801 4287 4746 5704 442 Size: 486 459 958 1753 Fragments arranged by size: 1753 1578 958 586 486 459 416 399 220 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7015 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2173 3434 2173 Size: 1261 5754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2837 2905 3168 3536 3619 3723 3866 5675 Size: 1598 68 263 368 83 104 143 1809 Cuts at: 5675 1239 Size: 2579 Fragments arranged by size: 2579 1809 1598 368 263 143 104 83 68 Bpu10I CC'TnA_GC Cuts at: 2904 2955 2904 Size: 51 6964 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 2722 5140 6005 6303 2513 Size: 209 2418 865 298 3225 Fragments arranged by size: 3225 2418 865 298 209 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2284 5678 882 Size: 617 785 3394 2219 Fragments arranged by size: 3394 2219 785 617 BsaAI yAC'GTr Cuts at: 493 1204 4806 493 Size: 711 3602 2702 Fragments arranged by size: 3602 2702 711 BsaBI GATnn'nnATC Cuts at: 7009 7009 Size: 7015 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2244 4051 4822 5204 Size: 53 83 186 936 711 1807 771 382 Cuts at: 5204 5839 275 Size: 635 1451 Fragments arranged by size: 1807 1451 936 771 711 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5448 5846 6120 6267 6678 5448 Size: 398 274 147 411 5785 Fragments arranged by size: 5785 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4139 4169 4267 4297 1178 Size: 30 165 30 2736 30 98 30 3896 Fragments arranged by size: 3896 2736 165 98 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 3670 4275 1186 Size: 1219 1265 605 3926 Fragments arranged by size: 3926 1265 1219 605 BseYI C'CCAG_C Cuts at: 2552 2677 2707 3135 4258 6218 6714 2552 Size: 125 30 428 1123 1960 496 2853 Fragments arranged by size: 2853 1960 1123 496 428 125 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3804 1163 Size: 573 2068 4374 Fragments arranged by size: 4374 2068 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2107 4465 5226 6254 1540 Size: 275 186 106 2358 761 1028 2301 Fragments arranged by size: 2358 2301 1028 761 275 186 106 BsiHKAI G_wGCw'C Cuts at: 3363 3415 3642 5076 5161 6232 3363 Size: 52 227 1434 85 1071 4146 Fragments arranged by size: 4146 1434 1071 227 85 52 BsmI GAATG_Cn' Cuts at: 4526 4619 4526 Size: 93 6922 BsmBI CGTCTCn'nnnn_ Cuts at: 2727 3554 2727 Size: 827 6188 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2708 3135 3363 3415 3642 3973 Size: 399 337 581 427 228 52 227 331 Cuts at: 3973 4117 4285 4334 5076 5161 6232 1391 Size: 144 168 49 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 581 427 399 337 331 228 227 168 144 85 52 49 BspEI T'CCGG_A Cuts at: 6678 6678 Size: 7015 BspHI T'CATG_A Cuts at: 4904 6634 4904 Size: 1730 5285 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2470 2895 4370 844 Size: 1626 425 1475 3489 Fragments arranged by size: 3489 1626 1475 425 BsrBI CCG'CTC Cuts at: 1394 1823 2091 2118 4177 4902 1394 Size: 429 268 27 2059 725 3507 Fragments arranged by size: 3507 2059 725 429 268 27 BsrDI GCAATG_nn' Cuts at: 65 1248 2394 5510 5684 65 Size: 1183 1146 3116 174 1396 Fragments arranged by size: 3116 1396 1183 1146 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 5659 5846 1700 Size: 110 198 3651 187 2869 Fragments arranged by size: 3651 2869 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7015 BssSI C'ACGA_G Cuts at: 5075 6087 5075 Size: 1012 6003 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7015 BstBI TT'CG_AA Cuts at: 5819 5819 Size: 7015 BstEII G'GTnAC_C Cuts at: 1184 3328 3856 1184 Size: 2144 528 4343 Fragments arranged by size: 4343 2144 528 BstXI CCAn_nnnn'nTGG Cuts at: 2620 2715 3809 2620 Size: 95 1094 5826 Fragments arranged by size: 5826 1094 95 BstYI r'GATC_y Cuts at: 1972 2215 3433 4345 4712 5097 5114 6566 6652 Size: 243 1218 912 367 385 17 1452 86 Cuts at: 6652 6664 7010 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1218 912 385 367 346 243 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2319 2319 Size: 7015 BtgI C'CryG_G Cuts at: 513 1065 2694 2804 3732 3870 513 Size: 552 1629 110 928 138 3658 Fragments arranged by size: 3658 1629 928 552 138 110 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6276 Fragments arranged by size: 6276 683 56 BtsI GCAGTG_nn' Cuts at: 2625 3405 4213 4531 5325 5345 2625 Size: 780 808 318 794 20 4295 Fragments arranged by size: 4295 808 794 780 318 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7015 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6980 DraI TTT'AAA Cuts at: 4319 4319 Size: 7015 DraIII CAC_nnn'GTG Cuts at: 3328 3979 3328 Size: 651 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6022 809 Size: 507 4706 1802 Fragments arranged by size: 4706 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 2500 2911 Size: 54 1425 50 275 18 274 396 411 Cuts at: 2911 3271 3544 4194 4462 5351 6980 8 Size: 360 273 650 268 889 1629 43 Fragments arranged by size: 1629 1425 889 650 411 396 360 275 274 273 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2104 4462 1537 Size: 275 292 2358 4090 Fragments arranged by size: 4090 2358 292 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4945 1353 Size: 3592 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5602 5976 6122 387 Size: 1064 138 4013 374 146 1280 Fragments arranged by size: 4013 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3413 3413 Size: 7015 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2837 2905 3061 3168 3536 3619 3723 Size: 393 1205 68 156 107 368 83 104 Cuts at: 3723 3866 4188 5078 5675 6462 6730 1239 Size: 143 322 890 597 787 268 1524 Fragments arranged by size: 1524 1205 890 787 597 393 368 322 268 156 143 107 104 83 68 EcoNI CCTnn'n_nnAGG Cuts at: 3578 3578 Size: 7015 EcoO109I rG'GnC_Cy Cuts at: 2036 2560 2830 2900 3583 2036 Size: 524 270 70 683 5468 Fragments arranged by size: 5468 683 524 270 70 EcoRI G'AATT_C Cuts at: 4324 4702 4324 Size: 378 6637 FspI TGC'GCA Cuts at: 3747 5521 3747 Size: 1774 5241 HaeII r_GCGC'y Cuts at: 1678 2247 3879 6162 1678 Size: 569 1632 2283 2531 Fragments arranged by size: 2531 2283 1632 569 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 3099 3131 3141 Size: 32 135 32 635 32 860 32 10 Cuts at: 3141 3173 3397 3429 3636 3650 3665 3668 3682 Size: 32 224 32 207 14 15 3 14 Cuts at: 3682 3697 4139 4171 4292 4324 5656 5688 5730 Size: 15 442 32 121 32 1332 32 42 Cuts at: 5730 5762 1373 Size: 32 2626 Fragments arranged by size: 2626 1332 860 635 442 224 207 135 121 42 32 32 32 32 32 32 32 32 32 32 15 15 14 14 10 3 HincII GTy'rAC Cuts at: 669 1657 1938 4359 4605 669 Size: 988 281 2421 246 3079 Fragments arranged by size: 3079 2421 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7015 HpaI GTT'AAC Cuts at: 4605 4605 Size: 7015 KasI G'GCGC_C Cuts at: 2243 2243 Size: 7015 KpnI G_GTAC'C Cuts at: 4340 4340 Size: 7015 MfeI C'AATT_G Cuts at: 4614 4614 Size: 7015 MluI A'CGCG_T Cuts at: 6891 6891 Size: 7015 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3064 6129 6313 1305 Size: 1759 3065 184 2007 Fragments arranged by size: 3065 2007 1759 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3273 4196 10 Size: 54 1425 343 1441 923 2829 Fragments arranged by size: 2829 1441 1425 923 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4973 5332 5491 518 Size: 648 3807 359 159 2042 Fragments arranged by size: 3807 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2533 2543 3266 3489 3743 4186 4431 5108 Size: 927 10 723 223 254 443 245 677 Cuts at: 5108 6256 6501 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 927 723 677 443 254 245 245 223 10 NaeI GCC'GGC Cuts at: 2010 2010 Size: 7015 NarI GG'CG_CC Cuts at: 2244 2244 Size: 7015 NcoI C'CATG_G Cuts at: 513 1065 3870 513 Size: 552 2805 3658 Fragments arranged by size: 3658 2805 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7015 NgoMIV G'CCGG_C Cuts at: 2008 2008 Size: 7015 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7015 NotI GC'GGCC_GC Cuts at: 4462 4462 Size: 7015 NspI r_CATG'y Cuts at: 1132 1229 3752 4299 4373 5918 1132 Size: 97 2523 547 74 1545 2229 Fragments arranged by size: 2523 2229 1545 547 97 74 PasI CC'CwG_GG Cuts at: 1348 3863 1348 Size: 2515 4500 PciI A'CATG_T Cuts at: 4295 5914 4295 Size: 1619 5396 PflMI CCAn_nnn'nTGG Cuts at: 1108 3269 3979 4124 1108 Size: 2161 710 145 3999 Fragments arranged by size: 3999 2161 710 145 PfoI T'CCnGG_A Cuts at: 3228 3228 Size: 7015 PmeI GTTT'AAAC Cuts at: 4319 4319 Size: 7015 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5084 5116 6597 6629 5084 Size: 32 1481 32 5470 Fragments arranged by size: 5470 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2036 2560 2830 3583 2036 Size: 524 270 753 5468 Fragments arranged by size: 5468 753 524 270 PshAI GACnn'nnGTC Cuts at: 1613 2160 2367 1613 Size: 547 207 6261 Fragments arranged by size: 6261 547 207 PsiI TTA'TAA Cuts at: 4585 4585 Size: 7015 PspOMI G'GGCC_C Cuts at: 3969 3969 Size: 7015 PstI C_TGCA'G Cuts at: 830 2934 4367 830 Size: 2104 1433 3478 Fragments arranged by size: 3478 2104 1433 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7015 PvuII CAG'CTG Cuts at: 2543 3266 3489 3743 2543 Size: 723 223 254 5815 Fragments arranged by size: 5815 723 254 223 SacI G_AGCT'C Cuts at: 3415 3415 Size: 7015 SalI G'TCGA_C Cuts at: 1936 4357 1936 Size: 2421 4594 SanDI GG'GwC_CC Cuts at: 2036 2036 Size: 7015 SbfI CC_TGCA'GG Cuts at: 2934 4367 2934 Size: 1433 5582 ScaI AGT'ACT Cuts at: 5263 6975 5263 Size: 1712 5303 SfcI C'TryA_G Cuts at: 826 1045 2049 2338 2930 3291 3738 4138 4363 Size: 219 1004 289 592 361 447 400 225 Cuts at: 4363 5498 6179 6370 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1004 681 592 447 400 361 289 225 219 191 SfoI GGC'GCC Cuts at: 2245 2245 Size: 7015 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7015 SmaI CCC'GGG Cuts at: 1872 2034 2041 2249 4342 1872 Size: 162 7 208 2093 4545 Fragments arranged by size: 4545 2093 208 162 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 2492 2701 5119 6020 6282 820 Size: 197 926 549 209 2418 901 262 1553 Fragments arranged by size: 2418 1553 926 901 549 262 209 197 SnaBI TAC'GTA Cuts at: 493 4806 493 Size: 4313 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7015 SphI G_CATG'C Cuts at: 1132 4373 1132 Size: 3241 3774 SspI AAT'ATT Cuts at: 5 52 4939 5 Size: 47 4887 2081 Fragments arranged by size: 4887 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7015 StyI C'CwwG_G Cuts at: 513 1065 3870 4454 513 Size: 552 2805 584 3074 Fragments arranged by size: 3074 2805 584 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3252 5042 5059 5212 5397 5842 325 Size: 1255 1672 1790 17 153 185 445 1498 Fragments arranged by size: 1790 1672 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5261 6973 96 Size: 274 80 33 51 1443 3284 1712 138 Fragments arranged by size: 3284 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3834 3866 4218 4250 6975 7007 3834 Size: 32 352 32 2725 32 3842 Fragments arranged by size: 3842 2725 352 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3587 1316 Size: 2271 4744 XbaI T'CTAG_A Cuts at: 4351 4351 Size: 7015 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3143 3594 3777 3143 Size: 451 183 6381 Fragments arranged by size: 6381 451 183 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7015 XmaI C'CCGG_G Cuts at: 1870 2032 2039 2247 4340 1870 Size: 162 7 208 2093 4545 Fragments arranged by size: 4545 2093 208 162 7 XmnI GAAnn'nnTTC Cuts at: 5144 5144 Size: 7015 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4823 276 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI