(Circular) (Six-base) MAPSORT of: pFN21AB8590.seq Check: 2093 from: 1 to: 5740 pFN21AB8590 5740 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3803 5187 5455 1632 Size: 2171 1384 268 1917 Fragments arranged by size: 2171 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 3483 5055 3483 Size: 1572 4168 ApaLI G'TGCA_C Cuts at: 2739 3797 4953 2739 Size: 1058 1156 3526 Fragments arranged by size: 3526 1156 1058 ApoI r'AATT_y Cuts at: 1493 2092 2373 3049 3275 3427 5536 1493 Size: 599 281 676 226 152 2109 1697 Fragments arranged by size: 2109 1697 676 599 281 226 152 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 3065 1870 Size: 73 1122 4545 Fragments arranged by size: 4545 1122 73 BamHI G'GATC_C Cuts at: 2160 3070 3437 2160 Size: 910 367 4463 Fragments arranged by size: 4463 910 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2819 3061 4516 618 Size: 325 217 627 1032 242 1455 1842 Fragments arranged by size: 1842 1455 1032 627 325 242 217 BanII G_rGCy'C Cuts at: 1391 2561 3059 1391 Size: 1170 498 4072 Fragments arranged by size: 4072 1170 498 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4769 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3931 3965 1903 Size: 34 1994 34 3678 Fragments arranged by size: 3678 1994 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2971 3632 4842 2971 Size: 661 1210 3869 Fragments arranged by size: 3869 1210 661 BclI T'GATC_A Cuts at: 1548 2236 1548 Size: 688 5052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4351 136 Size: 107 122 71 631 605 337 2342 1525 Fragments arranged by size: 2342 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 2743 3801 4957 1790 Size: 953 1058 1156 2573 Fragments arranged by size: 2573 1156 1058 953 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2709 3471 4429 Size: 416 53 83 399 24 1292 762 958 Cuts at: 4429 442 Size: 1753 Fragments arranged by size: 1753 1292 958 762 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 3042 4400 1239 Size: 1044 759 1358 2579 Fragments arranged by size: 2579 1358 1044 759 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2768 4403 882 Size: 617 1269 1635 2219 Fragments arranged by size: 2219 1635 1269 617 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3547 3929 4564 275 Size: 53 83 186 936 2014 382 635 1451 Fragments arranged by size: 2014 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2031 4173 4571 4845 4992 5403 2031 Size: 2142 398 274 147 411 2368 Fragments arranged by size: 2368 2142 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2395 2425 1178 Size: 30 165 30 992 30 4493 Fragments arranged by size: 4493 992 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2610 1186 Size: 1424 4316 BseYI C'CCAG_C Cuts at: 2059 4943 5439 2059 Size: 2884 496 2360 Fragments arranged by size: 2884 2360 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2628 2928 1163 Size: 573 892 300 3975 Fragments arranged by size: 3975 892 573 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3951 4979 1540 Size: 275 186 1189 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2743 3801 3886 4957 2743 Size: 1058 85 1071 3526 Fragments arranged by size: 3526 1071 1058 85 BsmI GAATG_Cn' Cuts at: 2532 3251 3344 2532 Size: 719 93 4928 Fragments arranged by size: 4928 719 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2561 2743 3059 3801 3886 4957 1391 Size: 399 771 182 316 742 85 1071 2174 Fragments arranged by size: 2174 1071 771 742 399 316 182 85 BspEI T'CCGG_A Cuts at: 2031 5403 2031 Size: 3372 2368 BspHI T'CATG_A Cuts at: 2457 2466 3629 5359 2457 Size: 9 1163 1730 2838 Fragments arranged by size: 2838 1730 1163 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3095 844 Size: 2251 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3627 1394 Size: 429 1804 3507 Fragments arranged by size: 3507 1804 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2847 4235 4409 65 Size: 1183 1599 1388 174 1396 Fragments arranged by size: 1599 1396 1388 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4384 4571 1700 Size: 110 2574 187 2869 Fragments arranged by size: 2869 2574 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5740 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstYI r'GATC_y Cuts at: 1972 2160 2786 3070 3437 3822 3839 5291 5377 Size: 188 626 284 367 385 17 1452 86 Cuts at: 5377 5389 5735 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 626 385 367 346 284 188 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2511 2811 513 Size: 552 1446 300 3442 Fragments arranged by size: 3442 1446 552 300 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 2616 3256 4050 4070 2616 Size: 640 794 20 4286 Fragments arranged by size: 4286 794 640 20 ClaI AT'CG_AT Cuts at: 1554 2213 1554 Size: 659 5081 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 2230 3044 2230 Size: 814 4926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4747 809 Size: 507 3431 1802 Fragments arranged by size: 3431 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2179 2700 3187 Size: 54 1425 50 275 18 349 521 487 Cuts at: 3187 4076 5705 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 521 487 349 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2649 3670 1353 Size: 1296 1021 3423 Fragments arranged by size: 3423 1296 1021 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 4701 4847 387 Size: 1064 138 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 3042 3803 4400 5187 5455 1239 Size: 393 651 759 761 597 787 268 1524 Fragments arranged by size: 1524 787 761 759 651 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2639 2639 Size: 5740 EcoRI G'AATT_C Cuts at: 2092 2373 3049 3427 2092 Size: 281 676 378 4405 Fragments arranged by size: 4405 676 378 281 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 4887 1678 Size: 3209 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4381 4413 4455 4487 1373 Size: 32 135 32 2809 32 42 32 2626 Fragments arranged by size: 2809 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2121 3084 3330 669 Size: 988 281 183 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 183 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 2121 3330 2121 Size: 1209 4531 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 2490 3339 2490 Size: 849 4891 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2497 4854 5038 1305 Size: 1192 2357 184 2007 Fragments arranged by size: 2357 2007 1192 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2181 2702 10 Size: 54 1425 343 349 521 3048 Fragments arranged by size: 3048 1425 521 349 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 2595 3698 4057 4216 518 Size: 648 1221 208 1103 359 159 2042 Fragments arranged by size: 2042 1221 1103 648 359 208 159 MspA1I CmG'CkG Cuts at: 1606 2672 3156 3833 4981 5226 1606 Size: 1066 484 677 1148 245 2120 Fragments arranged by size: 2120 1148 1066 677 484 245 NcoI C'CATG_G Cuts at: 513 1065 2511 513 Size: 552 1446 3742 Fragments arranged by size: 3742 1446 552 NdeI CA'TA_TG Cuts at: 387 2548 387 Size: 2161 3579 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 2872 3098 4643 1132 Size: 97 1643 226 1545 2229 Fragments arranged by size: 2229 1643 1545 226 97 PasI CC'CwG_GG Cuts at: 1348 2746 1348 Size: 1398 4342 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 2927 1108 Size: 1819 3921 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2639 2639 Size: 5740 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PstI C_TGCA'G Cuts at: 830 3092 830 Size: 2262 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 PvuII CAG'CTG Cuts at: 2672 2672 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 3088 4223 4904 5095 826 Size: 219 2043 1135 681 191 1471 Fragments arranged by size: 2043 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 3067 1872 Size: 1195 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3844 4745 5007 820 Size: 197 926 1901 901 262 1553 Fragments arranged by size: 1901 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 2631 3664 5 Size: 47 2579 1033 2081 Fragments arranged by size: 2579 2081 1033 47 StuI AGG'CCT Cuts at: 1517 2075 2688 1517 Size: 558 613 4569 Fragments arranged by size: 4569 613 558 StyI C'CwwG_G Cuts at: 513 1065 2070 2511 3179 513 Size: 552 1005 441 668 3074 Fragments arranged by size: 3074 1005 668 552 441 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2073 2473 2594 2726 3767 3784 3937 Size: 1255 493 400 121 132 1041 17 153 Cuts at: 3937 4122 4567 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1041 493 445 400 185 153 132 121 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 3986 5698 Size: 274 80 33 51 1443 414 1595 1712 Cuts at: 5698 96 Size: 138 Fragments arranged by size: 1712 1595 1443 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2152 2184 5700 5732 2152 Size: 32 3516 32 2160 Fragments arranged by size: 3516 2160 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 3065 1870 Size: 1195 4545 XmnI GAAnn'nnTTC Cuts at: 2377 2724 3869 2377 Size: 347 1145 4248 Fragments arranged by size: 4248 1145 347 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI