Restriction map for
pFN21AB8575
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FokI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeII
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 406 GTCTAC GT^[AC][GT]AC
AflIII 908 ACATGT A^C[GA][CT]GT
AlwI 357 GATCC GGATC(4/5)
Asp700I 372 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 755 CCCGGG C^[CT]CG[GA]G
BanI 112 GGTGCC G^G[CT][GA]CC
BbsI 567 GAAGAC GAAGAC(2/6)
BclI 423 TGATCA T^GATCA
BglI 664 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 966 GCTAAGC GC^T[ACGT]AGC
BpmI 196 CTCCAG CTGGAG(16/14)
Bpu1102I 966 GCTAAGC GC^T[ACGT]AGC
BpuAI 567 GAAGAC GAAGAC(2/6)
BsaBI 433 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 184 GTGCAG GTGCAG(16/14)
BsmBI 997 GAGACG CGTCTC(1/5)
BsoBI 755 CCCGGG C^[CT]CG[GA]G
BspLU11I 908 ACATGT A^CATGT
BsrBI 628 GAGCGG CCGCTC(-3/-3)
BstYI 356 AGATCC [GA]^GATC[CT]
CelII 966 GCTAAGC GC^T[ACGT]AGC
CfoI 640 GCGC GCG^C
DsaI 473 CCATGG C^C[GA][CT]GG
EaeI 1001 CGGCCA [CT]^GGCC[GA]
FbaI 423 TGATCA T^GATCA
HaeII 639 GGCGCT [GA]GCGC^[CT]
HapII 756 CCGG C^CGG
HhaI 640 GCGC GCG^C
HinP1I 640 GCGC G^CGC
HinfI 919 GACTC G^A[ACGT]TC
HpaII 756 CCGG C^CGG
MaeIII 508 GTAAC ^GT[ACGT]AC
MamI 433 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 356 AGATCC [GA]^GATC[CT]
MspI 756 CCGG C^CGG
NcoI 473 CCATGG C^CATGG
NheI 954 GCTAGC G^CTAGC
NspI 908 ACATGT [GA]CATG^[CT]
PleI 919 GACTC GAGTC(4/5)
PpuMI 461 AGGACCT [GA]G^G[AT]CC[CT]
SmaI 755 CCCGGG CCC^GGG
SpeI 232 ACTAGT A^CTAGT
TaqI 127 TCGA T^CGA
TthHB8I 127 TCGA T^CGA
XhoII 356 AGATCC [GA]^GATC[CT]
XmaCI 755 CCCGGG C^CCGGG
XmaI 755 CCCGGG C^CCGGG
XmnI 372 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 220 GTAC GT^AC
855 GTAC
Alw44I 67 GTGCAC G^TGCAC
388 GTGCAC
AlwNI 460 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
773 CAGATGCTG
ApaLI 67 GTGCAC G^TGCAC
388 GTGCAC
AspHI 67 GTGCAC G[AT]GC[AT]^C
388 GTGCAC
AvaII 285 GGTCC G^G[AT]CC
462 GGACC
BbvI 843 GCAGC GCAGC(8/12)
742 GCTGC
BcnI 755 CCCGG CC^[GC]GG
756 CCGGG
BfaI 233 CTAG C^TAG
955 CTAG
BsiHKAI 67 GTGCAC G[AT]GC[AT]^C
388 GTGCAC
BsiYI 480 CCCTGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
756 CCGGGAGAAGG
BslI 480 CCCTGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
756 CCGGGAGAAGG
BsrDI 582 CATTGC GCAATG(2/0)
864 CATTGC
DraII 338 AGGCCCT [GA]G^G[ACGT]CC[CT]
461 AGGACCT
EarI 55 CTCTTC CTCTTC(1/4)
79 GAAGAG
EcoO109I 338 AGGCCCT [GA]G^G[ACGT]CC[CT]
461 AGGACCT
EcoT14I 473 CCATGG C^C[AT][AT]GG
992 CCTTGG
FauI 504 GCGGG CCCGC(4/6)
925 GCGGG
HindIII 574 AAGCTT A^AGCTT
969 AAGCTT
Ksp632I 55 CTCTTC CTCTTC(1/4)
79 GAAGAG
MaeI 233 CTAG C^TAG
955 CTAG
MslI 789 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
909 CATGTTCATG
NciI 755 CCCGG CC^[GC]GG
756 CCGGG
PstI 381 CTGCAG CTGCA^G
841 CTGCAG
PvuII 62 CAGCTG CAG^CTG
844 CAGCTG
RsaI 220 GTAC GT^AC
855 GTAC
SfcI 381 CTGCAG C^T[GA][CT]AG
841 CTGCAG
StyI 473 CCATGG C^C[AT][AT]GG
992 CCTTGG
TseI 742 GCTGC G^C[AT]GC
843 GCAGC
Tsp509I 264 AATT ^AATT
732 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BmyI BsaJI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HphI ItaI MboI
MboII MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TspRI