(Circular) (Six-base) MAPSORT of: pFN21AB8575.seq Check: 2332 from: 1 to: 5722 pFN21AB8575 5722 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2412 3065 1937 Size: 475 653 4594 Fragments arranged by size: 4594 653 475 Acc65I G'GTAC_C Cuts at: 3043 3043 Size: 5722 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2846 3785 5169 5437 1632 Size: 1214 939 1384 268 1917 Fragments arranged by size: 1917 1384 1214 939 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 2913 4621 5598 1203 Size: 1710 1708 977 1327 Fragments arranged by size: 1710 1708 1327 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5722 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5722 AlwNI CAG_nnn'CTG Cuts at: 2470 2783 3465 5037 2470 Size: 313 682 1572 3155 Fragments arranged by size: 3155 1572 682 313 ApaLI G'TGCA_C Cuts at: 2072 2393 3779 4935 2072 Size: 321 1386 1156 2859 Fragments arranged by size: 2859 1386 1156 321 ApoI r'AATT_y Cuts at: 1493 3031 3257 3409 5518 1493 Size: 1538 226 152 2109 1697 Fragments arranged by size: 2109 1697 1538 226 152 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 2760 3047 1870 Size: 73 817 287 4545 Fragments arranged by size: 4545 817 287 73 BamHI G'GATC_C Cuts at: 3052 3419 3052 Size: 367 5355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 3043 4498 618 Size: 325 217 627 330 926 1455 1842 Fragments arranged by size: 1842 1455 926 627 330 325 217 BanII G_rGCy'C Cuts at: 1391 3041 1391 Size: 1650 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2579 928 Size: 971 680 4071 Fragments arranged by size: 4071 971 680 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 1903 Size: 34 1976 34 3678 Fragments arranged by size: 3678 1976 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3614 4824 3614 Size: 1210 4512 BclI T'GATC_A Cuts at: 1548 2428 1548 Size: 880 4842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2675 4333 136 Size: 107 122 71 631 605 1003 1658 1525 Fragments arranged by size: 1658 1525 1003 631 605 122 107 71 BglII A'GATC_T Cuts at: 5717 5717 Size: 5722 BlpI GC'TnA_GC Cuts at: 2972 3139 2972 Size: 167 5555 Bme1580I G_kGCm'C Cuts at: 1790 2076 2122 2397 3783 4939 1790 Size: 286 46 275 1386 1156 2573 Fragments arranged by size: 2573 1386 1156 286 275 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3453 4411 442 Size: 416 53 83 399 24 2036 958 1753 Fragments arranged by size: 2036 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2963 1056 Size: 1907 3815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 4382 1239 Size: 947 2196 2579 Fragments arranged by size: 2579 2196 947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3847 4712 5010 3847 Size: 865 298 4559 Fragments arranged by size: 4559 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4385 882 Size: 617 2886 2219 Fragments arranged by size: 2886 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 2442 5716 2442 Size: 3274 2448 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3529 3911 4546 275 Size: 53 83 186 936 1996 382 635 1451 Fragments arranged by size: 1996 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4155 4553 4827 4974 5385 4155 Size: 398 274 147 411 4492 Fragments arranged by size: 4492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2010 2040 2459 2489 1178 Size: 30 165 30 607 30 419 30 4411 Fragments arranged by size: 4411 607 419 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5722 BseYI C'CCAG_C Cuts at: 4925 5421 4925 Size: 496 5226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 1163 Size: 573 474 4675 Fragments arranged by size: 4675 573 474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3172 3933 4961 1540 Size: 275 186 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 2397 3783 3868 4939 2076 Size: 321 1386 85 1071 2859 Fragments arranged by size: 2859 1386 1071 321 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 BsmBI CGTCTCn'nnnn_ Cuts at: 2996 2996 Size: 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2122 2397 3041 3783 3868 4939 Size: 399 286 46 275 644 742 85 1071 Cuts at: 4939 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 644 399 286 275 85 46 BspEI T'CCGG_A Cuts at: 5385 5385 Size: 5722 BspHI T'CATG_A Cuts at: 3611 5341 3611 Size: 1730 3992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3077 844 Size: 2233 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2635 3609 1394 Size: 429 812 974 3507 Fragments arranged by size: 3507 974 812 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2586 2868 4217 4391 65 Size: 1183 1338 282 1349 174 1396 Fragments arranged by size: 1396 1349 1338 1183 282 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4366 4553 1700 Size: 110 2556 187 2869 Fragments arranged by size: 2869 2556 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5722 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 3782 4794 3782 Size: 1012 4710 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5722 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2361 3052 3419 3804 3821 5273 5359 5371 Size: 389 691 367 385 17 1452 86 12 Cuts at: 5371 5717 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 691 389 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2478 513 Size: 552 939 474 3757 Fragments arranged by size: 3757 939 552 474 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4983 Fragments arranged by size: 4983 683 56 BtsI GCAGTG_nn' Cuts at: 2307 2395 2793 2941 3238 4032 4052 2307 Size: 88 398 148 297 794 20 3977 Fragments arranged by size: 3977 794 398 297 148 88 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3006 3169 4058 Size: 54 1425 50 275 18 1176 163 889 Cuts at: 4058 5687 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1176 889 275 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2079 3652 1353 Size: 713 13 1573 3423 Fragments arranged by size: 3423 1573 713 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4309 4683 4829 387 Size: 1064 138 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2846 3785 4382 5169 5437 1239 Size: 393 554 660 939 597 787 268 1524 Fragments arranged by size: 1524 939 787 660 597 554 393 268 EcoO109I rG'GnC_Cy Cuts at: 2344 2467 2344 Size: 123 5599 EcoRI G'AATT_C Cuts at: 3031 3409 3031 Size: 378 5344 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2908 2992 2908 Size: 84 5638 FspI TGC'GCA Cuts at: 4228 4228 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 2648 4869 1678 Size: 970 2221 2531 Fragments arranged by size: 2531 2221 970 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 4363 4395 4437 Size: 32 135 32 887 32 1872 32 42 Cuts at: 4437 4469 1373 Size: 32 2626 Fragments arranged by size: 2626 1872 887 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3066 3312 669 Size: 988 281 1128 246 3079 Fragments arranged by size: 3079 1128 988 281 246 HindIII A'AGCT_T Cuts at: 748 2579 2974 748 Size: 1831 395 3496 Fragments arranged by size: 3496 1831 395 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 3047 3047 Size: 5722 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2901 4836 5020 1305 Size: 1596 1935 184 2007 Fragments arranged by size: 2007 1935 1596 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3900 Fragments arranged by size: 3900 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2798 2918 3680 4039 4198 518 Size: 648 1632 120 762 359 159 2042 Fragments arranged by size: 2042 1632 762 648 359 159 120 MspA1I CmG'CkG Cuts at: 1606 2069 2139 2851 3138 3815 4963 5208 1606 Size: 463 70 712 287 677 1148 245 2120 Fragments arranged by size: 2120 1148 712 677 463 287 245 70 NcoI C'CATG_G Cuts at: 513 1065 2004 2478 513 Size: 552 939 474 3757 Fragments arranged by size: 3757 939 552 474 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NheI G'CTAG_C Cuts at: 1052 2959 1052 Size: 1907 3815 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 2917 3080 4625 1132 Size: 97 1688 163 1545 2229 Fragments arranged by size: 2229 1688 1545 163 97 PasI CC'CwG_GG Cuts at: 1348 2486 1348 Size: 1138 4584 PciI A'CATG_T Cuts at: 2913 4621 2913 Size: 1708 4014 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5722 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3791 3823 5304 5336 3791 Size: 32 1481 32 4177 Fragments arranged by size: 4177 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2467 2467 Size: 5722 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PstI C_TGCA'G Cuts at: 830 2390 2850 3074 830 Size: 1560 460 224 3478 Fragments arranged by size: 3478 1560 460 224 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 PvuII CAG'CTG Cuts at: 2069 2851 2069 Size: 782 4940 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SfcI C'TryA_G Cuts at: 826 1045 2386 2846 3070 4205 4886 5077 826 Size: 219 1341 460 224 1135 681 191 1471 Fragments arranged by size: 1471 1341 1135 681 460 224 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 2762 3049 1872 Size: 890 287 4545 Fragments arranged by size: 4545 890 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 3826 4727 4989 820 Size: 197 926 1883 901 262 1553 Fragments arranged by size: 1883 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 2237 152 Size: 2085 3637 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 3646 5 Size: 47 3594 2081 Fragments arranged by size: 3594 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5722 StyI C'CwwG_G Cuts at: 513 1065 2004 2478 2997 3161 513 Size: 552 939 474 519 164 3074 Fragments arranged by size: 3074 939 552 519 474 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3749 3766 3919 4104 4549 325 Size: 1255 2169 17 153 185 445 1498 Fragments arranged by size: 2169 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2859 3968 5680 Size: 274 80 33 51 1443 882 1109 1712 Cuts at: 5680 96 Size: 138 Fragments arranged by size: 1712 1443 1109 882 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2010 2042 2227 2259 5682 5714 2010 Size: 32 185 32 3423 32 2018 Fragments arranged by size: 3423 2018 185 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5722 XmaI C'CCGG_G Cuts at: 1870 2760 3047 1870 Size: 890 287 4545 Fragments arranged by size: 4545 890 287 XmnI GAAnn'nnTTC Cuts at: 2381 3851 2381 Size: 1470 4252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI