Restriction map for
pFN21AB8575
(Linear) (Six-base) MAPSORT of: KIBB8575.seq Check: 3255 from: 1 to: 1017
KIBB8575.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 407 1017
Size: 407 610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1017
Size: 841 176
AflIII A'CryG_T
Cuts at: 0 908 1017
Size: 908 109
AlwNI CAG_nnn'CTG
Cuts at: 0 465 778 1017
Size: 465 313 239
Fragments arranged by size:
465 313 239
ApaLI G'TGCA_C
Cuts at: 0 67 388 1017
Size: 67 321 629
Fragments arranged by size:
629 321 67
AvaI C'yCGr_G
Cuts at: 0 755 1017
Size: 755 262
BanI G'GyrC_C
Cuts at: 0 112 1017
Size: 112 905
BbsI GAAGACnn'nnnn_
Cuts at: 0 574 1017
Size: 574 443
BclI T'GATC_A
Cuts at: 0 423 1017
Size: 423 594
BglI GCCn_nnn'nGGC
Cuts at: 0 670 1017
Size: 670 347
BlpI GC'TnA_GC
Cuts at: 0 967 1017
Size: 967 50
Bme1580I G_kGCm'C
Cuts at: 0 71 117 392 1017
Size: 71 46 275 625
Fragments arranged by size:
625 275 71 46
BmtI G_CTAG'C
Cuts at: 0 958 1017
Size: 958 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 1017
Size: 181 836
BsaBI GATnn'nnATC
Cuts at: 0 437 1017
Size: 437 580
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 35 454 484 1017
Size: 5 30 419 30 533
Fragments arranged by size:
533 419 30 30 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1017
Size: 205 812
BsiHKAI G_wGCw'C
Cuts at: 0 71 392 1017
Size: 71 321 625
Fragments arranged by size:
625 321 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 991 1017
Size: 991 26
Bsp1286I G_dGCh'C
Cuts at: 0 71 117 392 1017
Size: 71 46 275 625
Fragments arranged by size:
625 275 71 46
BsrBI CCG'CTC
Cuts at: 0 630 1017
Size: 630 387
BsrDI GCAATG_nn'
Cuts at: 0 581 863 1017
Size: 581 282 154
Fragments arranged by size:
581 282 154
BstYI r'GATC_y
Cuts at: 0 356 1017
Size: 356 661
BtgI C'CryG_G
Cuts at: 0 473 1017
Size: 473 544
BtsI GCAGTG_nn'
Cuts at: 0 302 390 788 936 1017
Size: 302 88 398 148 81
Fragments arranged by size:
398 302 148 88 81
EaeI y'GGCC_r
Cuts at: 0 1001 1017
Size: 1001 16
EarI CTCTTCn'nnn_
Cuts at: 0 61 74 1017
Size: 61 13 943
Fragments arranged by size:
943 61 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 841 1017
Size: 181 660 176
Fragments arranged by size:
660 181 176
EcoO109I rG'GnC_Cy
Cuts at: 0 339 462 1017
Size: 339 123 555
Fragments arranged by size:
555 339 123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 903 987 1017
Size: 903 84 30
Fragments arranged by size:
903 84 30
HaeII r_GCGC'y
Cuts at: 0 643 1017
Size: 643 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 1017
Size: 454 32 531
Fragments arranged by size:
531 454 32
HindIII A'AGCT_T
Cuts at: 0 574 969 1017
Size: 574 395 48
Fragments arranged by size:
574 395 48
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1017
Size: 896 121
MslI CAynn'nnrTG
Cuts at: 0 793 913 1017
Size: 793 120 104
Fragments arranged by size:
793 120 104
MspA1I CmG'CkG
Cuts at: 0 64 134 846 1017
Size: 64 70 712 171
Fragments arranged by size:
712 171 70 64
NcoI C'CATG_G
Cuts at: 0 473 1017
Size: 473 544
NheI G'CTAG_C
Cuts at: 0 954 1017
Size: 954 63
NspI r_CATG'y
Cuts at: 0 912 1017
Size: 912 105
PasI CC'CwG_GG
Cuts at: 0 481 1017
Size: 481 536
PciI A'CATG_T
Cuts at: 0 908 1017
Size: 908 109
PpuMI rG'GwC_Cy
Cuts at: 0 462 1017
Size: 462 555
PstI C_TGCA'G
Cuts at: 0 385 845 1017
Size: 385 460 172
Fragments arranged by size:
460 385 172
PvuII CAG'CTG
Cuts at: 0 64 846 1017
Size: 64 782 171
Fragments arranged by size:
782 171 64
SfcI C'TryA_G
Cuts at: 0 381 841 1017
Size: 381 460 176
Fragments arranged by size:
460 381 176
SmaI CCC'GGG
Cuts at: 0 757 1017
Size: 757 260
SpeI A'CTAG_T
Cuts at: 0 232 1017
Size: 232 785
StyI C'CwwG_G
Cuts at: 0 473 992 1017
Size: 473 519 25
Fragments arranged by size:
519 473 25
TatI w'GTAC_w
Cuts at: 0 854 1017
Size: 854 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5 37 222 254 1017
Size: 5 32 185 32 763
Fragments arranged by size:
763 185 32 32 5
XmaI C'CCGG_G
Cuts at: 0 755 1017
Size: 755 262
XmnI GAAnn'nnTTC
Cuts at: 0 376 1017
Size: 376 641
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaLI AvaI BanI BbsI
BclI BglI BlpI Bme1580I BmtI BpmI BsaBI BsaXI
BsgI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BstYI BtgI
BtsI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I
HindIII MmeI MslI MspA1I NcoI NheI NspI PasI
PciI PpuMI PstI PvuII SfcI SmaI SpeI StyI
TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BfrBI BglII BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XhoI ZraI