(Circular) (Six-base) MAPSORT of: pFN21AB8574.seq Check: 7024 from: 1 to: 7048 pFN21AB8574 7048 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4858 278 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2800 3799 4391 1937 Size: 863 999 592 4594 Fragments arranged by size: 4594 999 863 592 Acc65I G'GTAC_C Cuts at: 4369 4369 Size: 7048 AclI AA'CG_TT Cuts at: 1563 5175 5548 1563 Size: 3612 373 3063 Fragments arranged by size: 3612 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2832 3401 3597 3857 4018 5111 6495 6763 Size: 1200 569 196 260 161 1093 1384 268 Cuts at: 6763 1632 Size: 1917 Fragments arranged by size: 1917 1384 1200 1093 569 268 260 196 161 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6851 AflIII A'CryG_T Cuts at: 1203 5947 6924 1203 Size: 4744 977 1327 Fragments arranged by size: 4744 1327 977 AgeI A'CCGG_T Cuts at: 5879 5879 Size: 7048 AhdI GACnn_n'nnGTC Cuts at: 3795 5777 3795 Size: 1982 5066 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7048 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3707 1184 Size: 2523 4525 AlwNI CAG_nnn'CTG Cuts at: 2105 4013 4135 4791 6363 2105 Size: 1908 122 656 1572 2790 Fragments arranged by size: 2790 1908 1572 656 122 ApaI G_GGCC'C Cuts at: 4051 4187 4051 Size: 136 6912 ApaLI G'TGCA_C Cuts at: 5105 6261 5105 Size: 1156 5892 ApoI r'AATT_y Cuts at: 1493 2435 3008 3062 4336 4342 4357 4583 4735 Size: 942 573 54 1274 6 15 226 152 Cuts at: 4735 6844 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1274 942 573 226 152 54 15 6 AseI AT'TA_AT Cuts at: 160 1032 5602 160 Size: 872 4570 1606 Fragments arranged by size: 4570 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 AvaI C'yCGr_G Cuts at: 1870 1943 3503 4373 1870 Size: 73 1560 870 4545 Fragments arranged by size: 4545 1560 870 73 BamHI G'GATC_C Cuts at: 2827 4378 4745 2827 Size: 1551 367 5130 Fragments arranged by size: 5130 1551 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4159 4369 5824 618 Size: 325 217 627 2372 210 1455 1842 Fragments arranged by size: 2372 1842 1455 627 325 217 210 BanII G_rGCy'C Cuts at: 1391 3600 4051 4149 4187 4367 1391 Size: 2209 451 98 38 180 4072 Fragments arranged by size: 4072 2209 451 180 98 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2024 3296 928 Size: 971 125 1272 4680 Fragments arranged by size: 4680 1272 971 125 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5239 5273 1903 Size: 34 3302 34 3678 Fragments arranged by size: 3678 3302 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4940 6150 4940 Size: 1210 5838 BclI T'GATC_A Cuts at: 1548 2248 1548 Size: 700 6348 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4097 5659 136 Size: 107 122 71 631 605 2425 1562 1525 Fragments arranged by size: 2425 1562 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7043 7043 Size: 7048 BlpI GC'TnA_GC Cuts at: 4465 4465 Size: 7048 Bme1580I G_kGCm'C Cuts at: 1790 4051 4187 5109 6265 1790 Size: 2261 136 922 1156 2573 Fragments arranged by size: 2573 2261 1156 922 136 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2107 2127 4779 Size: 416 53 83 399 24 690 20 2652 Cuts at: 4779 5737 442 Size: 958 1753 Fragments arranged by size: 2652 1753 958 690 416 399 83 53 24 20 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7048 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3182 3298 3584 4136 4313 5708 1239 Size: 1943 116 286 552 177 1395 2579 Fragments arranged by size: 2579 1943 1395 552 286 177 116 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2902 5173 6038 6336 2902 Size: 2271 865 298 3614 Fragments arranged by size: 3614 2271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5711 882 Size: 617 4212 2219 Fragments arranged by size: 4212 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4839 493 Size: 711 3635 2702 Fragments arranged by size: 3635 2702 711 BsaBI GATnn'nnATC Cuts at: 7042 7042 Size: 7048 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4855 5237 5872 275 Size: 53 83 186 936 3322 382 635 1451 Fragments arranged by size: 3322 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5481 5879 6153 6300 6711 5481 Size: 398 274 147 411 5818 Fragments arranged by size: 5818 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2632 2662 1178 Size: 30 165 30 1229 30 5564 Fragments arranged by size: 5564 1229 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7048 BseYI C'CCAG_C Cuts at: 2037 4166 4187 4213 6251 6747 2037 Size: 2129 21 26 2038 496 2338 Fragments arranged by size: 2338 2129 2038 496 26 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2490 3149 4145 1163 Size: 573 754 659 996 4066 Fragments arranged by size: 4066 996 754 659 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4498 5259 6287 1540 Size: 275 186 2497 761 1028 2301 Fragments arranged by size: 2497 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3600 5109 5194 6265 3600 Size: 1509 85 1071 4383 Fragments arranged by size: 4383 1509 1071 85 BsmI GAATG_Cn' Cuts at: 4559 4652 4559 Size: 93 6955 BsmBI CGTCTCn'nnnn_ Cuts at: 3428 3428 Size: 7048 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3600 4051 4149 4187 4367 5109 5194 Size: 399 1810 451 98 38 180 742 85 Cuts at: 5194 6265 1391 Size: 1071 2174 Fragments arranged by size: 2174 1810 1071 742 451 399 180 98 85 38 BspEI T'CCGG_A Cuts at: 6711 6711 Size: 7048 BspHI T'CATG_A Cuts at: 4937 6667 4937 Size: 1730 5318 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4403 844 Size: 3559 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4935 1394 Size: 429 3112 3507 Fragments arranged by size: 3507 3112 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2397 2766 5543 5717 65 Size: 1183 1149 369 2777 174 1396 Fragments arranged by size: 2777 1396 1183 1149 369 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5692 5879 1700 Size: 110 3882 187 2869 Fragments arranged by size: 3882 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2657 96 Size: 2561 4487 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7048 BssSI C'ACGA_G Cuts at: 5108 6120 5108 Size: 1012 6036 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7048 BstBI TT'CG_AA Cuts at: 5852 5852 Size: 7048 BstEII G'GTnAC_C Cuts at: 1184 2363 1184 Size: 1179 5869 BstXI CCAn_nnnn'nTGG Cuts at: 3208 3208 Size: 7048 BstYI r'GATC_y Cuts at: 1972 2782 2827 3351 3654 3772 4029 4378 4745 Size: 810 45 524 303 118 257 349 367 Cuts at: 4745 5130 5147 6599 6685 6697 7043 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 810 524 385 367 349 346 303 257 118 86 45 17 12 BstZ17I GTA'TAC Cuts at: 2801 2801 Size: 7048 Bsu36I CC'TnA_GG Cuts at: 4155 4155 Size: 7048 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5557 Fragments arranged by size: 5557 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6309 Fragments arranged by size: 6309 683 56 BtsI GCAGTG_nn' Cuts at: 2580 3234 4564 5358 5378 2580 Size: 654 1330 794 20 4250 Fragments arranged by size: 4250 1330 794 654 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7048 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2489 2524 529 Size: 35 1925 35 5053 Fragments arranged by size: 5053 1925 35 35 DraI TTT'AAA Cuts at: 3097 4352 3097 Size: 1255 5793 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6055 809 Size: 507 4739 1802 Fragments arranged by size: 4739 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4495 5384 7013 Size: 54 1425 50 275 18 2665 889 1629 Cuts at: 7013 8 Size: 43 Fragments arranged by size: 2665 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4495 1537 Size: 275 2683 4090 Fragments arranged by size: 4090 2683 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 3034 3107 3573 4227 4978 1353 Size: 1197 484 73 466 654 751 3423 Fragments arranged by size: 3423 1197 751 654 484 466 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5635 6009 6155 387 Size: 1064 138 4046 374 146 1280 Fragments arranged by size: 4046 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3598 3598 Size: 7048 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2832 3182 3298 3401 3584 3597 3857 Size: 393 1200 350 116 103 183 13 260 Cuts at: 3857 4018 4136 4313 5111 5708 6495 6763 1239 Size: 161 118 177 798 597 787 268 1524 Fragments arranged by size: 1524 1200 798 787 597 393 350 268 260 183 177 161 118 116 103 13 EcoO109I rG'GnC_Cy Cuts at: 2513 4047 4183 4184 2513 Size: 1534 136 1 5377 Fragments arranged by size: 5377 1534 136 1 EcoRI G'AATT_C Cuts at: 4357 4735 4357 Size: 378 6670 FspI TGC'GCA Cuts at: 5554 5554 Size: 7048 HaeII r_GCGC'y Cuts at: 1678 3321 6195 1678 Size: 1643 2874 2531 Fragments arranged by size: 2874 2531 1643 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2288 2320 2372 2404 3538 Size: 32 135 32 716 32 52 32 1134 Cuts at: 3538 3557 3570 3589 3781 3813 5689 5721 5763 Size: 19 13 19 192 32 1876 32 42 Cuts at: 5763 5795 1373 Size: 32 2626 Fragments arranged by size: 2626 1876 1134 716 192 135 52 42 32 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 3800 4392 4638 669 Size: 988 281 1862 592 246 3079 Fragments arranged by size: 3079 1862 988 592 281 246 HindIII A'AGCT_T Cuts at: 748 2814 748 Size: 2066 4982 HpaI GTT'AAC Cuts at: 4638 4638 Size: 7048 KpnI G_GTAC'C Cuts at: 4373 4373 Size: 7048 MfeI C'AATT_G Cuts at: 4647 4647 Size: 7048 MluI A'CGCG_T Cuts at: 6924 6924 Size: 7048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3114 3202 6162 6346 1305 Size: 1809 88 2960 184 2007 Fragments arranged by size: 2960 2007 1809 184 88 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5226 Fragments arranged by size: 5226 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2169 5006 5365 5524 518 Size: 648 1003 2837 359 159 2042 Fragments arranged by size: 2837 2042 1003 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2570 4191 4464 5141 6289 6534 1606 Size: 964 1621 273 677 1148 245 2120 Fragments arranged by size: 2120 1621 1148 964 677 273 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5557 Fragments arranged by size: 5557 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7048 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7048 NotI GC'GGCC_GC Cuts at: 4495 4495 Size: 7048 NspI r_CATG'y Cuts at: 1132 1229 2029 2184 3151 3188 4406 5951 1132 Size: 97 800 155 967 37 1218 1545 2229 Fragments arranged by size: 2229 1545 1218 967 800 155 97 37 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7048 PciI A'CATG_T Cuts at: 5947 5947 Size: 7048 PflMI CCAn_nnn'nTGG Cuts at: 1108 4069 1108 Size: 2961 4087 PmeI GTTT'AAAC Cuts at: 4352 4352 Size: 7048 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5117 5149 6630 6662 5117 Size: 32 1481 32 5503 Fragments arranged by size: 5503 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2513 2513 Size: 7048 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7048 PsiI TTA'TAA Cuts at: 2124 4618 2124 Size: 2494 4554 PspOMI G'GGCC_C Cuts at: 4047 4183 4047 Size: 136 6912 PstI C_TGCA'G Cuts at: 830 2048 2575 3686 4201 4400 830 Size: 1218 527 1111 515 199 3478 Fragments arranged by size: 3478 1218 1111 527 515 199 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7048 PvuII CAG'CTG Cuts at: 2570 4191 2570 Size: 1621 5427 SacI G_AGCT'C Cuts at: 3600 3600 Size: 7048 SalI G'TCGA_C Cuts at: 1936 3798 4390 1936 Size: 1862 592 4594 Fragments arranged by size: 4594 1862 592 SapI GCTCTTCn'nnn_ Cuts at: 3107 3573 4227 3107 Size: 466 654 5928 Fragments arranged by size: 5928 654 466 SbfI CC_TGCA'GG Cuts at: 4400 4400 Size: 7048 ScaI AGT'ACT Cuts at: 5296 7008 5296 Size: 1712 5336 SfcI C'TryA_G Cuts at: 826 1045 2044 2571 2712 3682 4197 4396 5531 Size: 219 999 527 141 970 515 199 1135 Cuts at: 5531 6212 6403 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 999 970 681 527 515 219 199 191 141 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7048 SmaI CCC'GGG Cuts at: 1872 4375 1872 Size: 2503 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2917 5152 6053 6315 820 Size: 197 926 974 2235 901 262 1553 Fragments arranged by size: 2235 1553 974 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4839 493 Size: 4346 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7048 SphI G_CATG'C Cuts at: 1132 3151 3188 4406 1132 Size: 2019 37 1218 3774 Fragments arranged by size: 3774 2019 1218 37 SspI AAT'ATT Cuts at: 5 52 4972 5 Size: 47 4920 2081 Fragments arranged by size: 4920 2081 47 StuI AGG'CCT Cuts at: 1517 3617 4153 1517 Size: 2100 536 4412 Fragments arranged by size: 4412 2100 536 StyI C'CwwG_G Cuts at: 513 1065 2004 2987 3306 3357 4308 4487 513 Size: 552 939 983 319 51 951 179 3074 Fragments arranged by size: 3074 983 951 939 552 319 179 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5075 5092 5245 5430 5875 325 Size: 1255 3495 17 153 185 445 1498 Fragments arranged by size: 3495 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2657 5294 7006 Size: 274 80 33 51 1443 680 2637 1712 Cuts at: 7006 96 Size: 138 Fragments arranged by size: 2637 1712 1443 680 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 7008 7040 2042 Size: 32 4934 32 2050 Fragments arranged by size: 4934 2050 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7048 XbaI T'CTAG_A Cuts at: 4384 4384 Size: 7048 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7048 XmaI C'CCGG_G Cuts at: 1870 4373 1870 Size: 2503 4545 XmnI GAAnn'nnTTC Cuts at: 5177 5177 Size: 7048 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4856 276 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI