(Circular) (Six-base) MAPSORT of: pFN21AB8467.seq Check: 6344 from: 1 to: 6052 pFN21AB8467 6052 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3036 3395 1937 Size: 1099 359 4594 Fragments arranged by size: 4594 1099 359 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4115 5499 5767 1632 Size: 2483 1384 268 1917 Fragments arranged by size: 2483 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5855 AflIII A'CryG_T Cuts at: 1203 4951 5928 1203 Size: 3748 977 1327 Fragments arranged by size: 3748 1327 977 AgeI A'CCGG_T Cuts at: 4883 4883 Size: 6052 AhdI GACnn_n'nnGTC Cuts at: 4781 4781 Size: 6052 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6052 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 2076 Size: 32 6020 AlwNI CAG_nnn'CTG Cuts at: 2886 3795 5367 2886 Size: 909 1572 3571 Fragments arranged by size: 3571 1572 909 ApaI G_GGCC'C Cuts at: 2746 3172 2746 Size: 426 5626 ApaLI G'TGCA_C Cuts at: 4109 5265 4109 Size: 1156 4896 ApoI r'AATT_y Cuts at: 1493 3254 3347 3361 3587 3739 5848 1493 Size: 1761 93 14 226 152 2109 1697 Fragments arranged by size: 2109 1761 1697 226 152 93 14 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 2193 2316 2923 3095 3377 1870 Size: 73 250 123 607 172 282 4545 Fragments arranged by size: 4545 607 282 250 172 123 73 BamHI G'GATC_C Cuts at: 3382 3749 3382 Size: 367 5685 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2782 2974 3373 4828 618 Size: 325 217 627 995 192 399 1455 1842 Fragments arranged by size: 1842 1455 995 627 399 325 217 192 BanII G_rGCy'C Cuts at: 1391 2073 2556 2746 2898 3172 3371 1391 Size: 682 483 190 152 274 199 4072 Fragments arranged by size: 4072 682 483 274 199 190 152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3279 928 Size: 971 1380 3701 Fragments arranged by size: 3701 1380 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4243 4277 1903 Size: 34 50 34 2222 34 3678 Fragments arranged by size: 3678 2222 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3944 5154 3944 Size: 1210 4842 BclI T'GATC_A Cuts at: 1548 2280 1548 Size: 732 5320 BfrBI ATG'CAT Cuts at: 2016 2016 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2403 3209 4663 Size: 107 122 71 631 605 731 806 1454 Cuts at: 4663 136 Size: 1525 Fragments arranged by size: 1525 1454 806 731 631 605 122 107 71 BglII A'GATC_T Cuts at: 2209 6047 2209 Size: 3838 2214 BlpI GC'TnA_GC Cuts at: 3137 3469 3137 Size: 332 5720 Bme1580I G_kGCm'C Cuts at: 1790 2193 2438 2746 2787 2979 3172 4113 5269 Size: 403 245 308 41 192 193 941 1156 Cuts at: 5269 1790 Size: 2573 Fragments arranged by size: 2573 1156 941 403 308 245 193 192 41 BmgBI CAC'GTC Cuts at: 1318 2971 1318 Size: 1653 4399 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3783 4741 442 Size: 416 53 83 399 24 2366 958 1753 Fragments arranged by size: 2366 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2127 1056 Size: 1071 4981 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2525 3308 4712 1239 Size: 995 291 783 1404 2579 Fragments arranged by size: 2579 1404 995 783 291 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2103 4177 5042 5340 2103 Size: 2074 865 298 2815 Fragments arranged by size: 2815 2074 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2539 3096 4715 882 Size: 617 1040 557 1619 2219 Fragments arranged by size: 2219 1619 1040 617 557 BsaAI yAC'GTr Cuts at: 493 1204 2029 3843 493 Size: 711 825 1814 2702 Fragments arranged by size: 2702 1814 825 711 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3859 4241 4876 275 Size: 53 83 186 936 2326 382 635 1451 Fragments arranged by size: 2326 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4485 4883 5157 5304 5715 4485 Size: 398 274 147 411 4822 Fragments arranged by size: 4822 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5827 Fragments arranged by size: 5827 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6052 BseYI C'CCAG_C Cuts at: 2064 2521 5255 5751 2064 Size: 457 2734 496 2365 Fragments arranged by size: 2734 2365 496 457 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3137 1163 Size: 573 1401 4078 Fragments arranged by size: 4078 1401 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3087 3502 4263 5291 1540 Size: 275 186 1086 415 761 1028 2301 Fragments arranged by size: 2301 1086 1028 761 415 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 4113 4198 5269 2073 Size: 2040 85 1071 2856 Fragments arranged by size: 2856 2040 1071 85 BsmI GAATG_Cn' Cuts at: 2018 3563 3656 2018 Size: 1545 93 4414 Fragments arranged by size: 4414 1545 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2193 2438 2556 2746 2787 2898 Size: 399 283 120 245 118 190 41 111 Cuts at: 2898 2979 3172 3371 4113 4198 5269 1391 Size: 81 193 199 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 245 199 193 190 120 118 111 85 81 41 BspEI T'CCGG_A Cuts at: 5715 5715 Size: 6052 BspHI T'CATG_A Cuts at: 2202 3941 5671 2202 Size: 1739 1730 2583 Fragments arranged by size: 2583 1739 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3407 844 Size: 2563 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3939 1394 Size: 429 2116 3507 Fragments arranged by size: 3507 2116 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2062 2182 3153 4547 4721 65 Size: 1183 814 120 971 1394 174 1396 Fragments arranged by size: 1396 1394 1183 971 814 174 120 BsrFI r'CCGG_y Cuts at: 1700 1810 4696 4883 1700 Size: 110 2886 187 2869 Fragments arranged by size: 2886 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 4112 5124 4112 Size: 1012 5040 BstAPI GCAn_nnn'nTGC Cuts at: 794 2061 794 Size: 1267 4785 BstBI TT'CG_AA Cuts at: 4856 4856 Size: 6052 BstEII G'GTnAC_C Cuts at: 1184 2812 1184 Size: 1628 4424 BstXI CCAn_nnnn'nTGG Cuts at: 2426 2426 Size: 6052 BstYI r'GATC_y Cuts at: 1972 2209 3382 3749 4134 4151 5603 5689 5701 Size: 237 1173 367 385 17 1452 86 12 Cuts at: 5701 6047 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1173 385 367 346 237 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3087 3147 513 Size: 552 2022 60 3418 Fragments arranged by size: 3418 2022 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5313 Fragments arranged by size: 5313 683 56 BtsI GCAGTG_nn' Cuts at: 2550 2718 3568 4362 4382 2550 Size: 168 850 794 20 4220 Fragments arranged by size: 4220 850 794 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3136 3171 529 Size: 35 2572 35 3410 Fragments arranged by size: 3410 2572 35 35 DraI TTT'AAA Cuts at: 3356 3356 Size: 6052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5059 809 Size: 507 3743 1802 Fragments arranged by size: 3743 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2868 3108 3150 Size: 54 1425 50 275 18 1038 240 42 Cuts at: 3150 3499 4388 6017 8 Size: 349 889 1629 43 Fragments arranged by size: 1629 1425 1038 889 349 275 240 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 2267 3982 1353 Size: 725 189 1715 3423 Fragments arranged by size: 3423 1715 725 189 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4639 5013 5159 387 Size: 1064 138 3050 374 146 1280 Fragments arranged by size: 3050 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2071 2071 Size: 6052 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2525 3308 4115 4712 5499 5767 Size: 393 602 291 783 807 597 787 268 Cuts at: 5767 1239 Size: 1524 Fragments arranged by size: 1524 807 787 783 602 597 393 291 268 EcoNI CCTnn'n_nnAGG Cuts at: 3110 3290 3110 Size: 180 5872 EcoO109I rG'GnC_Cy Cuts at: 2742 2743 2742 Size: 1 6051 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3229 3229 Size: 6052 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 2668 5199 1678 Size: 990 2531 2531 Fragments arranged by size: 2531 2531 990 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 2375 2407 4693 Size: 32 135 32 557 32 214 32 2286 Cuts at: 4693 4725 4767 4799 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2286 557 214 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3396 3642 669 Size: 988 281 1458 246 3079 Fragments arranged by size: 3079 1458 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6052 HpaI GTT'AAC Cuts at: 3642 3642 Size: 6052 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 3651 3651 Size: 6052 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5166 5350 1305 Size: 3861 184 2007 Fragments arranged by size: 3861 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3110 10 Size: 54 1425 343 1278 2952 Fragments arranged by size: 2952 1425 1278 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3225 4010 4369 4528 518 Size: 648 2059 785 359 159 2042 Fragments arranged by size: 2059 2042 785 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2278 2809 3121 3468 4145 5293 5538 1606 Size: 672 531 312 347 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 531 347 312 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5500 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NheI G'CTAG_C Cuts at: 1052 2123 1052 Size: 1071 4981 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NsiI A_TGCA'T Cuts at: 2018 2018 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 2189 2956 3136 3410 4955 1132 Size: 97 960 767 180 274 1545 2229 Fragments arranged by size: 2229 1545 960 767 274 180 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6052 PciI A'CATG_T Cuts at: 4951 4951 Size: 6052 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6052 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PmlI CAC'GTG Cuts at: 2029 2029 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 5634 5666 4121 Size: 32 1481 32 4507 Fragments arranged by size: 4507 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3042 3089 1613 Size: 1429 47 4576 Fragments arranged by size: 4576 1429 47 PsiI TTA'TAA Cuts at: 3622 3622 Size: 6052 PspOMI G'GGCC_C Cuts at: 2742 3168 2742 Size: 426 5626 PstI C_TGCA'G Cuts at: 830 2403 2545 3404 830 Size: 1573 142 859 3478 Fragments arranged by size: 3478 1573 859 142 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 PvuII CAG'CTG Cuts at: 2278 2278 Size: 6052 SacI G_AGCT'C Cuts at: 2073 2073 Size: 6052 SalI G'TCGA_C Cuts at: 1936 3394 1936 Size: 1458 4594 SapI GCTCTTCn'nnn_ Cuts at: 2078 2267 2078 Size: 189 5863 SbfI CC_TGCA'GG Cuts at: 2403 3404 2403 Size: 1001 5051 ScaI AGT'ACT Cuts at: 4300 6012 4300 Size: 1712 4340 SexAI A'CCwGG_T Cuts at: 2352 2778 2352 Size: 426 5626 SfcI C'TryA_G Cuts at: 826 1045 2223 2399 2541 3400 4535 5216 5407 Size: 219 1178 176 142 859 1135 681 191 Cuts at: 5407 826 Size: 1471 Fragments arranged by size: 1471 1178 1135 859 681 219 191 176 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6052 SmaI CCC'GGG Cuts at: 1872 2195 2318 3097 3379 1872 Size: 323 123 779 282 4545 Fragments arranged by size: 4545 779 323 282 123 SmlI C'TyrA_G Cuts at: 820 1017 1943 2118 4156 5057 5319 820 Size: 197 926 175 2038 901 262 1553 Fragments arranged by size: 2038 1553 926 901 262 197 175 SnaBI TAC'GTA Cuts at: 493 3843 493 Size: 3350 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 2956 3136 3410 1132 Size: 1824 180 274 3774 Fragments arranged by size: 3774 1824 274 180 SspI AAT'ATT Cuts at: 5 52 3976 5 Size: 47 3924 2081 Fragments arranged by size: 3924 2081 47 StuI AGG'CCT Cuts at: 1517 3164 1517 Size: 1647 4405 StyI C'CwwG_G Cuts at: 513 1065 2979 3111 3491 513 Size: 552 1914 132 380 3074 Fragments arranged by size: 3074 1914 552 380 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2591 4079 4096 4249 4434 4879 325 Size: 1255 1011 1488 17 153 185 445 1498 Fragments arranged by size: 1498 1488 1255 1011 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2937 4298 6010 Size: 274 80 33 51 1443 960 1361 1712 Cuts at: 6010 96 Size: 138 Fragments arranged by size: 1712 1443 1361 960 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6012 6044 6012 Size: 32 6020 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6052 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2426 2905 2426 Size: 479 5573 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6052 XmaI C'CCGG_G Cuts at: 1870 2193 2316 3095 3377 1870 Size: 323 123 779 282 4545 Fragments arranged by size: 4545 779 323 282 123 XmnI GAAnn'nnTTC Cuts at: 4181 4181 Size: 6052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI