(Circular) (Six-base) MAPSORT of: pFN21AB8464.seq Check: 4146 from: 1 to: 7084 pFN21AB8464 7084 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4168 4168 Size: 7084 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4894 278 Size: 53 83 186 936 3358 2468 Fragments arranged by size: 3358 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4427 1937 Size: 2490 4594 Acc65I G'GTAC_C Cuts at: 4405 4405 Size: 7084 AclI AA'CG_TT Cuts at: 1563 5211 5584 1563 Size: 3648 373 3063 Fragments arranged by size: 3648 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3672 3711 4079 5147 6531 6799 1632 Size: 2040 39 368 1068 1384 268 1917 Fragments arranged by size: 2040 1917 1384 1068 368 268 39 AfeI AGC'GCT Cuts at: 2942 2942 Size: 7084 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6887 AflIII A'CryG_T Cuts at: 1203 5983 6960 1203 Size: 4780 977 1327 Fragments arranged by size: 4780 1327 977 AgeI A'CCGG_T Cuts at: 5915 5915 Size: 7084 AhdI GACnn_n'nnGTC Cuts at: 5813 5813 Size: 7084 AleI CACnn'nnGTG Cuts at: 1166 3728 1166 Size: 2562 4522 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7084 AlwNI CAG_nnn'CTG Cuts at: 2386 2548 3218 4183 4827 6399 2386 Size: 162 670 965 644 1572 3071 Fragments arranged by size: 3071 1572 965 670 644 162 ApaI G_GGCC'C Cuts at: 2923 2923 Size: 7084 ApaLI G'TGCA_C Cuts at: 5141 6297 5141 Size: 1156 5928 ApoI r'AATT_y Cuts at: 1493 2201 2756 4393 4619 4771 6880 1493 Size: 708 555 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 708 555 226 152 AseI AT'TA_AT Cuts at: 160 1032 5638 160 Size: 872 4606 1606 Fragments arranged by size: 4606 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7084 AvaI C'yCGr_G Cuts at: 1870 1943 2712 4409 1870 Size: 73 769 1697 4545 Fragments arranged by size: 4545 1697 769 73 BamHI G'GATC_C Cuts at: 2214 4414 4781 2214 Size: 2200 367 4517 Fragments arranged by size: 4517 2200 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2538 3610 3889 3979 Size: 325 217 627 242 509 1072 279 90 Cuts at: 3979 4135 4405 5860 618 Size: 156 270 1455 1842 Fragments arranged by size: 1842 1455 1072 627 509 325 279 270 242 217 156 90 BanII G_rGCy'C Cuts at: 1391 2061 2923 4044 4403 1391 Size: 670 862 1121 359 4072 Fragments arranged by size: 4072 1121 862 670 359 BbeI G_GCGC'C Cuts at: 2542 2542 Size: 7084 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4067 4180 928 Size: 971 2168 113 3832 Fragments arranged by size: 3832 2168 971 113 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3314 3348 5275 5309 1903 Size: 34 50 34 1293 34 1927 34 3678 Fragments arranged by size: 3678 1927 1293 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4976 6186 4976 Size: 1210 5874 BclI T'GATC_A Cuts at: 1548 1548 Size: 7084 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3522 5695 Size: 107 122 71 631 605 337 1513 2173 Cuts at: 5695 136 Size: 1525 Fragments arranged by size: 2173 1525 1513 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2655 7079 2655 Size: 4424 2660 BlpI GC'TnA_GC Cuts at: 3414 4501 3414 Size: 1087 5997 Bme1580I G_kGCm'C Cuts at: 1790 2034 2052 2433 2923 3111 3735 3894 4140 Size: 244 18 381 490 188 624 159 246 Cuts at: 4140 5145 6301 1790 Size: 1005 1156 2573 Fragments arranged by size: 2573 1156 1005 624 490 381 246 244 188 159 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7084 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3261 4815 5773 Size: 416 53 83 399 24 1844 1554 958 Cuts at: 5773 442 Size: 1753 Fragments arranged by size: 1844 1753 1554 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7084 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4063 4063 Size: 7084 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 3138 3195 3207 3341 3554 3741 3978 Size: 888 1011 57 12 134 213 187 237 Cuts at: 3978 5744 1239 Size: 1766 2579 Fragments arranged by size: 2579 1766 1011 888 237 213 187 134 57 12 Bpu10I CC'TnA_GC Cuts at: 2322 2322 Size: 7084 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2675 4343 5209 6074 6372 2675 Size: 1668 866 865 298 3387 Fragments arranged by size: 3387 1668 866 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2414 4219 5747 882 Size: 617 915 1805 1528 2219 Fragments arranged by size: 2219 1805 1528 915 617 BsaAI yAC'GTr Cuts at: 493 1204 4875 493 Size: 711 3671 2702 Fragments arranged by size: 3671 2702 711 BsaBI GATnn'nnATC Cuts at: 3183 3231 7078 3183 Size: 48 3847 3189 Fragments arranged by size: 3847 3189 48 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2539 4891 5273 5908 Size: 53 83 186 936 1006 2352 382 635 Cuts at: 5908 275 Size: 1451 Fragments arranged by size: 2352 1451 1006 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5517 5915 6189 6336 6747 5517 Size: 398 274 147 411 5854 Fragments arranged by size: 5854 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2972 3002 1178 Size: 30 165 30 1569 30 5260 Fragments arranged by size: 5260 1569 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2130 2433 2804 2924 3011 3038 3488 3491 Size: 944 303 371 120 87 27 450 3 Cuts at: 3491 4100 4103 1186 Size: 609 3 4167 Fragments arranged by size: 4167 944 609 450 371 303 120 87 27 3 3 BseYI C'CCAG_C Cuts at: 2376 2553 2914 3036 3540 3644 3704 3988 6287 Size: 177 361 122 504 104 60 284 2299 Cuts at: 6287 6783 2376 Size: 496 2677 Fragments arranged by size: 2677 2299 504 496 361 284 177 122 104 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 2088 1163 Size: 573 306 46 6159 Fragments arranged by size: 6159 573 306 46 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 4534 5295 6323 1540 Size: 275 186 12 2521 761 1028 2301 Fragments arranged by size: 2521 2301 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 5145 5230 6301 5145 Size: 85 1071 5928 Fragments arranged by size: 5928 1071 85 BsmI GAATG_Cn' Cuts at: 4595 4688 4595 Size: 93 6991 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2052 2061 2433 2923 3111 3735 Size: 399 244 18 9 372 490 188 624 Cuts at: 3735 3894 4044 4140 4403 5145 5230 6301 1391 Size: 159 150 96 263 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 624 490 399 372 263 244 188 159 150 96 85 18 9 BspEI T'CCGG_A Cuts at: 6747 6747 Size: 7084 BspHI T'CATG_A Cuts at: 4237 4973 6703 4237 Size: 736 1730 4618 Fragments arranged by size: 4618 1730 736 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4168 4439 844 Size: 3324 271 3489 Fragments arranged by size: 3489 3324 271 BsrBI CCG'CTC Cuts at: 1394 1823 2806 3418 4971 1394 Size: 429 983 612 1553 3507 Fragments arranged by size: 3507 1553 983 612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2460 5579 5753 65 Size: 1183 1212 3119 174 1396 Fragments arranged by size: 3119 1396 1212 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 5728 5915 1700 Size: 110 198 3720 187 2869 Fragments arranged by size: 3720 2869 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7084 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7084 BssSI C'ACGA_G Cuts at: 5144 6156 5144 Size: 1012 6072 BstAPI GCAn_nnn'nTGC Cuts at: 794 2029 2386 3106 794 Size: 1235 357 720 4772 Fragments arranged by size: 4772 1235 720 357 BstBI TT'CG_AA Cuts at: 5888 5888 Size: 7084 BstEII G'GTnAC_C Cuts at: 1184 3502 1184 Size: 2318 4766 BstXI CCAn_nnnn'nTGG Cuts at: 2182 3607 4312 2182 Size: 1425 705 4954 Fragments arranged by size: 4954 1425 705 BstYI r'GATC_y Cuts at: 1972 2214 2655 3261 3480 4414 4781 5166 5183 Size: 242 441 606 219 934 367 385 17 Cuts at: 5183 6635 6721 6733 7079 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 934 606 441 385 367 346 242 219 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2052 4029 513 Size: 552 987 1977 3568 Fragments arranged by size: 3568 1977 987 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3372 487 Size: 683 56 2146 4199 Fragments arranged by size: 4199 2146 683 56 BtsI GCAGTG_nn' Cuts at: 4600 5394 5414 4600 Size: 794 20 6270 Fragments arranged by size: 6270 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7084 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7049 DraI TTT'AAA Cuts at: 4388 4388 Size: 7084 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6091 809 Size: 507 4775 1802 Fragments arranged by size: 4775 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2184 2526 Size: 54 1425 50 275 18 180 174 342 Cuts at: 2526 3514 4329 4531 5420 7049 8 Size: 988 815 202 889 1629 43 Fragments arranged by size: 1629 1425 988 889 815 342 275 202 180 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 4531 1537 Size: 275 198 2521 4090 Fragments arranged by size: 4090 2521 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2621 2781 5014 1353 Size: 1268 160 2233 3423 Fragments arranged by size: 3423 2233 1268 160 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2323 2588 3035 5671 6045 6191 Size: 1064 138 734 265 447 2636 374 146 Cuts at: 6191 387 Size: 1280 Fragments arranged by size: 2636 1280 1064 734 447 374 265 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 3138 3195 3207 3341 3554 3672 Size: 393 495 1011 57 12 134 213 118 Cuts at: 3672 3711 3741 3978 4079 5147 5744 6531 6799 Size: 39 30 237 101 1068 597 787 268 Cuts at: 6799 1239 Size: 1524 Fragments arranged by size: 1524 1068 1011 787 597 495 393 268 237 213 134 118 101 57 39 30 12 EcoNI CCTnn'n_nnAGG Cuts at: 2933 2933 Size: 7084 EcoO109I rG'GnC_Cy Cuts at: 2919 2919 Size: 7084 EcoRI G'AATT_C Cuts at: 4393 4771 4393 Size: 378 6706 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2266 2266 Size: 7084 FspI TGC'GCA Cuts at: 3293 4100 5590 3293 Size: 807 1490 4787 Fragments arranged by size: 4787 1490 807 FspAI rTGC'GCAy Cuts at: 3293 3293 Size: 7084 HaeII r_GCGC'y Cuts at: 1678 2542 2944 6231 1678 Size: 864 402 3287 2531 Fragments arranged by size: 3287 2531 864 402 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 2972 3004 3503 Size: 32 135 32 887 32 481 32 499 Cuts at: 3503 3535 4199 4231 4292 4324 5725 5757 5799 Size: 32 664 32 61 32 1401 32 42 Cuts at: 5799 5831 1373 Size: 32 2626 Fragments arranged by size: 2626 1401 887 664 499 481 135 61 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2164 4428 4674 669 Size: 988 281 226 2264 246 3079 Fragments arranged by size: 3079 2264 988 281 246 226 HindIII A'AGCT_T Cuts at: 748 748 Size: 7084 HpaI GTT'AAC Cuts at: 4674 4674 Size: 7084 KasI G'GCGC_C Cuts at: 2538 2538 Size: 7084 KpnI G_GTAC'C Cuts at: 4409 4409 Size: 7084 MfeI C'AATT_G Cuts at: 4683 4683 Size: 7084 MluI A'CGCG_T Cuts at: 6960 6960 Size: 7084 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2242 2890 4329 6198 6382 1305 Size: 937 648 1439 1869 184 2007 Fragments arranged by size: 2007 1869 1439 937 648 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 2528 3516 4331 10 Size: 54 1425 343 354 342 988 815 2763 Fragments arranged by size: 2763 1425 988 815 354 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 3728 3944 4226 4250 5042 5401 5560 Size: 648 2562 216 282 24 792 359 159 Cuts at: 5560 518 Size: 2042 Fragments arranged by size: 2562 2042 792 648 359 282 216 159 24 MspA1I CmG'CkG Cuts at: 1606 2386 2557 3040 3328 4500 5177 6325 6570 Size: 780 171 483 288 1172 677 1148 245 Cuts at: 6570 1606 Size: 2120 Fragments arranged by size: 2120 1172 1148 780 677 483 288 245 171 NaeI GCC'GGC Cuts at: 2010 2010 Size: 7084 NarI GG'CG_CC Cuts at: 2539 2539 Size: 7084 NcoI C'CATG_G Cuts at: 513 1065 4029 513 Size: 552 2964 3568 Fragments arranged by size: 3568 2964 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7084 NgoMIV G'CCGG_C Cuts at: 2008 2008 Size: 7084 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7084 NotI GC'GGCC_GC Cuts at: 4531 4531 Size: 7084 NspI r_CATG'y Cuts at: 1132 1229 3292 4147 4255 4442 5987 1132 Size: 97 2063 855 108 187 1545 2229 Fragments arranged by size: 2229 2063 1545 855 187 108 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7084 PciI A'CATG_T Cuts at: 5983 5983 Size: 7084 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7084 PfoI T'CCnGG_A Cuts at: 2760 3570 2760 Size: 810 6274 PmeI GTTT'AAAC Cuts at: 4388 4388 Size: 7084 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2441 2473 4076 4108 5153 5185 6666 6698 2441 Size: 32 1603 32 1045 32 1481 32 2827 Fragments arranged by size: 2827 1603 1481 1045 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7084 PsiI TTA'TAA Cuts at: 4654 4654 Size: 7084 PspOMI G'GGCC_C Cuts at: 2919 2919 Size: 7084 PstI C_TGCA'G Cuts at: 830 4261 4436 830 Size: 3431 175 3478 Fragments arranged by size: 3478 3431 175 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7084 PvuII CAG'CTG Cuts at: 2386 2557 3040 2386 Size: 171 483 6430 Fragments arranged by size: 6430 483 171 SalI G'TCGA_C Cuts at: 1936 4426 1936 Size: 2490 4594 SbfI CC_TGCA'GG Cuts at: 4261 4436 4261 Size: 175 6909 ScaI AGT'ACT Cuts at: 5332 7044 5332 Size: 1712 5372 SexAI A'CCwGG_T Cuts at: 3376 4209 3376 Size: 833 6251 SfcI C'TryA_G Cuts at: 826 1045 3768 4257 4274 4432 5567 6248 6439 Size: 219 2723 489 17 158 1135 681 191 Cuts at: 6439 826 Size: 1471 Fragments arranged by size: 2723 1471 1135 681 489 219 191 158 17 SfoI GGC'GCC Cuts at: 2540 2540 Size: 7084 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7084 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7084 SmaI CCC'GGG Cuts at: 1872 4411 1872 Size: 2539 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2690 4358 5188 6089 6351 820 Size: 197 926 747 1668 830 901 262 1553 Fragments arranged by size: 1668 1553 926 901 830 747 262 197 SnaBI TAC'GTA Cuts at: 493 4875 493 Size: 4382 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7084 SphI G_CATG'C Cuts at: 1132 4255 4442 1132 Size: 3123 187 3774 Fragments arranged by size: 3774 3123 187 SspI AAT'ATT Cuts at: 5 52 2158 5008 5 Size: 47 2106 2850 2081 Fragments arranged by size: 2850 2106 2081 47 StuI AGG'CCT Cuts at: 1517 3606 1517 Size: 2089 4995 StyI C'CwwG_G Cuts at: 513 1065 2346 4029 4523 513 Size: 552 1281 1683 494 3074 Fragments arranged by size: 3074 1683 1281 552 494 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2390 2683 3068 3142 3733 3757 5111 Size: 1255 810 293 385 74 591 24 1354 Cuts at: 5111 5128 5281 5466 5911 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1354 1255 810 591 445 385 293 185 153 74 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2646 3058 5330 Size: 274 80 33 51 1443 669 412 2272 Cuts at: 5330 7042 96 Size: 1712 138 Fragments arranged by size: 2272 1712 1443 669 412 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2717 2749 7044 7076 2717 Size: 32 4295 32 2725 Fragments arranged by size: 4295 2725 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3161 4211 1316 Size: 1845 1050 4189 Fragments arranged by size: 4189 1845 1050 XbaI T'CTAG_A Cuts at: 4420 4420 Size: 7084 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3883 3883 Size: 7084 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7084 XmaI C'CCGG_G Cuts at: 1870 4409 1870 Size: 2539 4545 XmnI GAAnn'nnTTC Cuts at: 5213 5213 Size: 7084 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4892 276 Size: 53 83 186 936 3358 2468 Fragments arranged by size: 3358 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI