(Circular) (Six-base) MAPSORT of: pFN21AB8460.seq Check: 8200 from: 1 to: 5461 pFN21AB8460 5461 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2804 1937 Size: 867 4594 Acc65I G'GTAC_C Cuts at: 2782 2782 Size: 5461 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2614 3524 4908 5176 1632 Size: 982 910 1384 268 1917 Fragments arranged by size: 1917 1384 982 910 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5264 AflIII A'CryG_T Cuts at: 1203 4360 5337 1203 Size: 3157 977 1327 Fragments arranged by size: 3157 1327 977 AgeI A'CCGG_T Cuts at: 4292 4292 Size: 5461 AhdI GACnn_n'nnGTC Cuts at: 4190 4190 Size: 5461 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2603 1184 Size: 1419 4042 AlwNI CAG_nnn'CTG Cuts at: 2473 2686 3204 4776 2473 Size: 213 518 1572 3158 Fragments arranged by size: 3158 1572 518 213 ApaLI G'TGCA_C Cuts at: 3518 4674 3518 Size: 1156 4305 ApoI r'AATT_y Cuts at: 1493 2770 2996 3148 5257 1493 Size: 1277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 226 152 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2237 2435 2757 2786 1870 Size: 73 294 198 322 29 4545 Fragments arranged by size: 4545 322 294 198 73 29 BamHI G'GATC_C Cuts at: 2791 3158 2791 Size: 367 5094 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2782 4237 618 Size: 325 217 627 995 1455 1842 Fragments arranged by size: 1842 1455 995 627 325 217 BanII G_rGCy'C Cuts at: 1391 2442 2499 2651 2780 1391 Size: 1051 57 152 129 4072 Fragments arranged by size: 4072 1051 152 129 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2620 928 Size: 971 721 3769 Fragments arranged by size: 3769 971 721 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 1903 Size: 34 1715 34 3678 Fragments arranged by size: 3678 1715 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2198 3353 4563 2198 Size: 1155 1210 3096 Fragments arranged by size: 3096 1210 1155 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4072 136 Size: 107 122 71 631 605 2400 1525 Fragments arranged by size: 2400 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2143 5456 2143 Size: 3313 2148 BlpI GC'TnA_GC Cuts at: 2493 2878 2493 Size: 385 5076 Bme1580I G_kGCm'C Cuts at: 1790 3522 4678 1790 Size: 1732 1156 2573 Fragments arranged by size: 2573 1732 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2244 2321 3192 Size: 416 53 83 399 24 827 77 871 Cuts at: 3192 4150 442 Size: 958 1753 Fragments arranged by size: 1753 958 871 827 416 399 83 77 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2591 2701 4121 1239 Size: 875 477 110 1420 2579 Fragments arranged by size: 2579 1420 875 477 110 Bpu10I CC'TnA_GC Cuts at: 2441 2441 Size: 5461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3586 4451 4749 3586 Size: 865 298 4298 Fragments arranged by size: 4298 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2252 4124 882 Size: 617 753 1872 2219 Fragments arranged by size: 2219 1872 753 617 BsaAI yAC'GTr Cuts at: 493 1204 2186 3252 493 Size: 711 982 1066 2702 Fragments arranged by size: 2702 1066 982 711 BsaBI GATnn'nnATC Cuts at: 5455 5455 Size: 5461 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3268 3650 4285 275 Size: 53 83 186 936 1735 382 635 1451 Fragments arranged by size: 1735 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2655 3894 4292 4566 4713 5124 2655 Size: 1239 398 274 147 411 2992 Fragments arranged by size: 2992 1239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5236 Fragments arranged by size: 5236 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 1186 Size: 1084 4377 BseYI C'CCAG_C Cuts at: 2272 2466 4664 5160 2272 Size: 194 2198 496 2573 Fragments arranged by size: 2573 2198 496 194 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 1163 Size: 573 573 4315 Fragments arranged by size: 4315 573 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2911 3672 4700 1540 Size: 275 186 910 761 1028 2301 Fragments arranged by size: 2301 1028 910 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 2223 3522 3607 4678 2118 Size: 105 1299 85 1071 2901 Fragments arranged by size: 2901 1299 1071 105 85 BsmI GAATG_Cn' Cuts at: 2972 3065 2972 Size: 93 5368 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2223 2442 2499 2651 2780 3522 Size: 399 328 105 219 57 152 129 742 Cuts at: 3522 3607 4678 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 219 152 129 105 85 57 BspEI T'CCGG_A Cuts at: 5124 5124 Size: 5461 BspHI T'CATG_A Cuts at: 2736 3350 5080 2736 Size: 614 1730 3117 Fragments arranged by size: 3117 1730 614 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2571 2816 844 Size: 1727 245 3489 Fragments arranged by size: 3489 1727 245 BsrBI CCG'CTC Cuts at: 1394 1823 3348 1394 Size: 429 1525 3507 Fragments arranged by size: 3507 1525 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3956 4130 65 Size: 1183 2708 174 1396 Fragments arranged by size: 2708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4105 4292 1700 Size: 110 2295 187 2869 Fragments arranged by size: 2869 2295 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5461 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5461 BssSI C'ACGA_G Cuts at: 3521 4533 3521 Size: 1012 4449 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5461 BstBI TT'CG_AA Cuts at: 4265 4265 Size: 5461 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5461 BstYI r'GATC_y Cuts at: 1972 2143 2791 3158 3543 3560 5012 5098 5110 Size: 171 648 367 385 17 1452 86 12 Cuts at: 5110 5456 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 648 385 367 346 171 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2264 2687 2264 Size: 423 5038 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3970 Fragments arranged by size: 3970 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2585 487 Size: 683 56 1359 3363 Fragments arranged by size: 3363 1359 683 56 BtsI GCAGTG_nn' Cuts at: 2221 2977 3771 3791 2221 Size: 756 794 20 3891 Fragments arranged by size: 3891 794 756 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5426 DraI TTT'AAA Cuts at: 2765 2765 Size: 5461 DraIII CAC_nnn'GTG Cuts at: 2617 2617 Size: 5461 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2334 2509 Size: 54 1425 50 275 18 292 212 175 Cuts at: 2509 2908 3797 5426 8 Size: 399 889 1629 43 Fragments arranged by size: 1629 1425 889 399 292 275 212 175 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2908 1537 Size: 275 1096 4090 Fragments arranged by size: 4090 1096 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3391 1353 Size: 2038 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4048 4422 4568 387 Size: 1064 138 2459 374 146 1280 Fragments arranged by size: 2459 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2591 2614 2701 3524 4121 4908 Size: 393 482 477 23 87 823 597 787 Cuts at: 4908 5176 1239 Size: 268 1524 Fragments arranged by size: 1524 823 787 597 482 477 393 268 87 23 EcoNI CCTnn'n_nnAGG Cuts at: 2357 2690 2357 Size: 333 5128 EcoO109I rG'GnC_Cy Cuts at: 2241 2517 2683 2241 Size: 276 166 5019 Fragments arranged by size: 5019 276 166 EcoRI G'AATT_C Cuts at: 2770 3148 2770 Size: 378 5083 FspI TGC'GCA Cuts at: 2702 3967 2702 Size: 1265 4196 HaeII r_GCGC'y Cuts at: 1678 2236 2353 4608 1678 Size: 558 117 2255 2531 Fragments arranged by size: 2531 2255 558 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4102 4134 4176 4208 1373 Size: 32 135 32 2530 32 42 32 2626 Fragments arranged by size: 2626 2530 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3051 669 Size: 988 281 867 246 3079 Fragments arranged by size: 3079 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KpnI G_GTAC'C Cuts at: 2786 2786 Size: 5461 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4575 4759 1305 Size: 3270 184 2007 Fragments arranged by size: 3270 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2124 10 Size: 54 1425 343 292 3347 Fragments arranged by size: 3347 1425 343 292 54 MslI CAynn'nnrTG Cuts at: 518 1166 3419 3778 3937 518 Size: 648 2253 359 159 2042 Fragments arranged by size: 2253 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 2470 2752 2877 3554 4702 4947 1606 Size: 488 376 282 125 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 488 376 282 245 125 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3970 Fragments arranged by size: 3970 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5461 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2908 2908 Size: 5461 NspI r_CATG'y Cuts at: 1132 1229 2819 4364 1132 Size: 97 1590 1545 2229 Fragments arranged by size: 2229 1590 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5461 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 2197 2371 2473 1108 Size: 1089 174 102 4096 Fragments arranged by size: 4096 1089 174 102 PfoI T'CCnGG_A Cuts at: 2169 2717 2169 Size: 548 4913 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2438 2470 2438 Size: 32 5429 PstI C_TGCA'G Cuts at: 830 2228 2295 2415 2582 2813 830 Size: 1398 67 120 167 231 3478 Fragments arranged by size: 3478 1398 231 167 120 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 PvuII CAG'CTG Cuts at: 2094 2470 2752 2094 Size: 376 282 4803 Fragments arranged by size: 4803 376 282 SalI G'TCGA_C Cuts at: 1936 2803 1936 Size: 867 4594 SbfI CC_TGCA'GG Cuts at: 2813 2813 Size: 5461 ScaI AGT'ACT Cuts at: 3709 5421 3709 Size: 1712 3749 SfcI C'TryA_G Cuts at: 826 1045 2085 2224 2291 2411 2578 2809 3944 Size: 219 1040 139 67 120 167 231 1135 Cuts at: 3944 4625 4816 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1040 681 231 219 191 167 139 120 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5461 SmaI CCC'GGG Cuts at: 1872 2239 2759 2788 1872 Size: 367 520 29 4545 Fragments arranged by size: 4545 520 367 29 SmlI C'TyrA_G Cuts at: 820 1017 1943 3565 4466 4728 820 Size: 197 926 1622 901 262 1553 Fragments arranged by size: 1622 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2186 3252 493 Size: 1693 1066 2702 Fragments arranged by size: 2702 1693 1066 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2819 1132 Size: 1687 3774 SspI AAT'ATT Cuts at: 5 52 3385 5 Size: 47 3333 2081 Fragments arranged by size: 3333 2081 47 StuI AGG'CCT Cuts at: 1517 2196 1517 Size: 679 4782 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 2191 2358 2900 513 Size: 552 939 115 72 167 542 3074 Fragments arranged by size: 3074 939 552 542 167 115 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3488 3505 3658 3843 4288 325 Size: 1255 1908 17 153 185 445 1498 Fragments arranged by size: 1908 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3707 5419 96 Size: 274 80 33 51 1443 1730 1712 138 Fragments arranged by size: 1730 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5421 5453 5421 Size: 32 5429 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5461 XbaI T'CTAG_A Cuts at: 2797 2797 Size: 5461 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2167 Size: 5461 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5461 XmaI C'CCGG_G Cuts at: 1870 2237 2757 2786 1870 Size: 367 520 29 4545 Fragments arranged by size: 4545 520 367 29 XmnI GAAnn'nnTTC Cuts at: 3590 3590 Size: 5461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI