Restriction map for
pFN21AB8458
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BbeI BbrPI BcgI BclI BfaI
BfrI BlnI BsaBI BsaI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MscI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 382 GACGTC GACGT^C
AcsI 567 GAATTT [GA]^AATT[CT]
AcyI 382 GACGTC G[GA]^CG[CT]C
AlwNI 13 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 691 CCTGAGG CC^T[ACGT]AGG
ApoI 567 GAATTT [GA]^AATT[CT]
Asp700I 40 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 814 GAGCTC G[AT]GC[AT]^C
BamHI 148 GGATCC G^GATCC
BanI 426 GGTGCC G^G[CT][GA]CC
BanII 814 GAGCTC G[GA]GC[CT]^C
BbvCI 868 GCTGAGG CCTCAGC(-5/-2)
BciVI 314 GGATAC GTATCC(6/5)
BglI 61 GCCATCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 777 AGATCT A^GATCT
BlpI 348 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 348 GCTCAGC GC^T[ACGT]AGC
BsaAI 280 TACGTG [CT]AC^GT[GA]
BsaHI 382 GACGTC G[GA]^CG[CT]C
BsgI 970 CTGCAC GTGCAG(16/14)
BsiHKAI 814 GAGCTC G[AT]GC[AT]^C
BsrDI 75 CATTGC GCAATG(2/0)
BssSI 929 CTCGTG CACGAG(-5/-1)
Bsu36I 691 CCTGAGG CC^T[ACGT]AGG
CelII 348 GCTCAGC GC^T[ACGT]AGC
DsaI 644 CCACGG C^C[GA][CT]GG
EaeI 68 CGGCCA [CT]^GGCC[GA]
Eco81I 691 CCTGAGG CC^T[ACGT]AGG
HgaI 865 GACGC GACGC(5/10)
Hin1I 382 GACGTC G[GA]^CG[CT]C
HincII 499 GTTGAC GT[CT]^[GA]AC
HindII 499 GTTGAC GT[CT]^[GA]AC
HinfI 415 GACTC G^A[ACGT]TC
MaeIII 32 GTGAC ^GT[ACGT]AC
MspA1I 16 CAGCTG C[AC]G^C[GT]G
NaeI 443 GCCGGC GCC^GGC
NgoMIV 443 GCCGGC G^CCGGC
PflMI 707 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 415 GACTC GAGTC(4/5)
PvuII 16 CAGCTG CAG^CTG
SacI 814 GAGCTC GAGCT^C
SapI 339 GAAGAGC GCTCTTC(1/4)
SfcI 993 CTACAG C^T[GA][CT]AG
Tsp45I 32 GTGAC ^GT[GC]AC
Van91I 707 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 272 CCAAAGTCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 40 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 277 GTCTAC GT^[AC][GT]AC
1000 GTCTAC
AccII 455 CGCG CG^CG
765 CGCG
AccIII 48 TCCGGA T^CCGGA
386 TCCGGA
AfaI 156 GTAC GT^AC
251 GTAC
Aor51HI 212 AGCGCT AGC^GCT
909 AGCGCT
BbsI 135 GAAGAC GAAGAC(2/6)
736 GAAGAC
BpuAI 135 GAAGAC GAAGAC(2/6)
736 GAAGAC
BsaWI 48 TCCGGA [AT]^CCGG[AT]
386 TCCGGA
BseAI 48 TCCGGA T^CCGGA
386 TCCGGA
BsmFI 863 GGGAC GGGAC(10/14)
480 GTCCC
BspEI 48 TCCGGA T^CCGGA
386 TCCGGA
BsrFI 357 GCCGGT [GA]^CCGG[CT]
443 GCCGGC
BstUI 455 CGCG CG^CG
765 CGCG
Cfr10I 357 GCCGGT [GA]^CCGG[CT]
443 GCCGGC
Eco47III 212 AGCGCT AGC^GCT
909 AGCGCT
HphI 132 GGTGA GGTGA(8/7)
104 TCACC
MroI 48 TCCGGA T^CCGGA
386 TCCGGA
MslI 126 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
762 CATCGCGGTG
MvnI 455 CGCG CG^CG
765 CGCG
RsaI 156 GTAC GT^AC
251 GTAC
TaqI 86 TCGA T^CGA
128 TCGA
Tsp509I 568 AATT ^AATT
856 AATT
TthHB8I 86 TCGA T^CGA
128 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI TspRI XhoII