Restriction map for pFN21AB8458
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BbeI        BbrPI       BcgI        BclI        BfaI        
BfrI        BlnI        BsaBI       BsaI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MluNI       MscI        
MseI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TfiI        Tru9I       Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         382  GACGTC               GACGT^C
AcsI          567  GAATTT               [GA]^AATT[CT]
AcyI          382  GACGTC               G[GA]^CG[CT]C
AlwNI          13  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          691  CCTGAGG              CC^T[ACGT]AGG
ApoI          567  GAATTT               [GA]^AATT[CT]
Asp700I        40  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         814  GAGCTC               G[AT]GC[AT]^C
BamHI         148  GGATCC               G^GATCC
BanI          426  GGTGCC               G^G[CT][GA]CC
BanII         814  GAGCTC               G[GA]GC[CT]^C
BbvCI         868  GCTGAGG              CCTCAGC(-5/-2)
BciVI         314  GGATAC               GTATCC(6/5)
BglI           61  GCCATCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         777  AGATCT               A^GATCT
BlpI          348  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      348  GCTCAGC              GC^T[ACGT]AGC
BsaAI         280  TACGTG               [CT]AC^GT[GA]
BsaHI         382  GACGTC               G[GA]^CG[CT]C
BsgI          970  CTGCAC               GTGCAG(16/14)
BsiHKAI       814  GAGCTC               G[AT]GC[AT]^C
BsrDI          75  CATTGC               GCAATG(2/0)
BssSI         929  CTCGTG               CACGAG(-5/-1)
Bsu36I        691  CCTGAGG              CC^T[ACGT]AGG
CelII         348  GCTCAGC              GC^T[ACGT]AGC
DsaI          644  CCACGG               C^C[GA][CT]GG
EaeI           68  CGGCCA               [CT]^GGCC[GA]
Eco81I        691  CCTGAGG              CC^T[ACGT]AGG
HgaI          865  GACGC                GACGC(5/10)
Hin1I         382  GACGTC               G[GA]^CG[CT]C
HincII        499  GTTGAC               GT[CT]^[GA]AC
HindII        499  GTTGAC               GT[CT]^[GA]AC
HinfI         415  GACTC                G^A[ACGT]TC
MaeIII         32  GTGAC                ^GT[ACGT]AC
MspA1I         16  CAGCTG               C[AC]G^C[GT]G
NaeI          443  GCCGGC               GCC^GGC
NgoMIV        443  GCCGGC               G^CCGGC
PflMI         707  CCACCAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          415  GACTC                GAGTC(4/5)
PvuII          16  CAGCTG               CAG^CTG
SacI          814  GAGCTC               GAGCT^C
SapI          339  GAAGAGC              GCTCTTC(1/4)
SfcI          993  CTACAG               C^T[GA][CT]AG
Tsp45I         32  GTGAC                ^GT[GC]AC
Van91I        707  CCACCAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          272  CCAAAGTCTACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           40  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          277  GTCTAC               GT^[AC][GT]AC
             1000  GTCTAC              
AccII         455  CGCG                 CG^CG
              765  CGCG                
AccIII         48  TCCGGA               T^CCGGA
              386  TCCGGA              
AfaI          156  GTAC                 GT^AC
              251  GTAC                
Aor51HI       212  AGCGCT               AGC^GCT
              909  AGCGCT              
BbsI          135  GAAGAC               GAAGAC(2/6)
              736  GAAGAC              
BpuAI         135  GAAGAC               GAAGAC(2/6)
              736  GAAGAC              
BsaWI          48  TCCGGA               [AT]^CCGG[AT]
              386  TCCGGA              
BseAI          48  TCCGGA               T^CCGGA
              386  TCCGGA              
BsmFI         863  GGGAC                GGGAC(10/14)
              480  GTCCC               
BspEI          48  TCCGGA               T^CCGGA
              386  TCCGGA              
BsrFI         357  GCCGGT               [GA]^CCGG[CT]
              443  GCCGGC              
BstUI         455  CGCG                 CG^CG
              765  CGCG                
Cfr10I        357  GCCGGT               [GA]^CCGG[CT]
              443  GCCGGC              
Eco47III      212  AGCGCT               AGC^GCT
              909  AGCGCT              
HphI          132  GGTGA                GGTGA(8/7)
              104  TCACC               
MroI           48  TCCGGA               T^CCGGA
              386  TCCGGA              
MslI          126  CATCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              762  CATCGCGGTG          
MvnI          455  CGCG                 CG^CG
              765  CGCG                
RsaI          156  GTAC                 GT^AC
              251  GTAC                
TaqI           86  TCGA                 T^CGA
              128  TCGA                
Tsp509I       568  AATT                 ^AATT
              856  AATT                
TthHB8I        86  TCGA                 T^CGA
              128  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
Ksp632I     MaeII       MboI        MboII       MflI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       TseI        TspRI       XhoII       
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