Restriction map for pFN21AB8458
 (Linear) (Six-base) MAPSORT of: KIBB8458.seq  Check: 1456  from: 1  to: 1017



KIBB8458.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     414    1017 
   Size:        414     603
 
AatII G_ACGT'C

Cuts at:      0     386    1017 
   Size:        386     631
 
AccI GT'mk_AC

Cuts at:      0     278    1001    1017 
   Size:        278     723      16

  Fragments arranged by size: 

                 723     278      16
 
AfeI AGC'GCT

Cuts at:      0     214     911    1017 
   Size:        214     697     106

  Fragments arranged by size: 

                 697     214     106
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18    1017 
   Size:         18     999
 
ApoI r'AATT_y

Cuts at:      0     567    1017 
   Size:        567     450
 
BamHI G'GATC_C

Cuts at:      0     148    1017 
   Size:        148     869
 
BanI G'GyrC_C

Cuts at:      0     426    1017 
   Size:        426     591
 
BanII G_rGCy'C

Cuts at:      0     818    1017 
   Size:        818     199
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     142     743    1017 
   Size:        142     601     274

  Fragments arranged by size: 

                 601     274     142
 
BbvCI CC'TCA_GC

Cuts at:      0     869    1017 
   Size:        869     148
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     308    1017 
   Size:        308     709
 
BglI GCCn_nnn'nGGC

Cuts at:      0      67    1017 
   Size:         67     950
 
BglII A'GATC_T

Cuts at:      0     777    1017 
   Size:        777     240
 
BlpI GC'TnA_GC

Cuts at:      0     349    1017 
   Size:        349     668
 
Bme1580I G_kGCm'C

Cuts at:      0     243     431     631    1017 
   Size:        243     188     200     386

  Fragments arranged by size: 

                 386     243     200     188
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     580     599     643     832    1017 
   Size:        226     354      19      44     189     185

  Fragments arranged by size: 

                 354     226     189     185      44      19
 
Bpu10I CC'TnA_GC

Cuts at:      0     869    1017 
   Size:        869     148
 
BsaAI yAC'GTr

Cuts at:      0     282    1017 
   Size:        282     735
 
BsaHI Gr'CG_yC

Cuts at:      0     383    1017 
   Size:        383     634
 
BsaWI w'CCGG_w

Cuts at:      0      48     386    1017 
   Size:         48     338     631

  Fragments arranged by size: 

                 631     338      48
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     223     586     709    1017 
   Size:        103     120     363     123     308

  Fragments arranged by size: 

                 363     308     123     120     103
 
BseYI C'CCAG_C

Cuts at:      0      11     267    1017 
   Size:         11     256     750

  Fragments arranged by size: 

                 750     256      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     955    1017 
   Size:        955      62
 
BsiHKAI G_wGCw'C

Cuts at:      0     818    1017 
   Size:        818     199
 
Bsp1286I G_dGCh'C

Cuts at:      0     243     431     631     818    1017 
   Size:        243     188     200     187     199

  Fragments arranged by size: 

                 243     200     199     188     187
 
BspEI T'CCGG_A

Cuts at:      0      48     386    1017 
   Size:         48     338     631

  Fragments arranged by size: 

                 631     338      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     327     414     815    1017 
   Size:        327      87     401     202

  Fragments arranged by size: 

                 401     327     202      87
 
BsrDI GCAATG_nn'

Cuts at:      0      74    1017 
   Size:         74     943
 
BsrFI r'CCGG_y

Cuts at:      0     357     443    1017 
   Size:        357      86     574

  Fragments arranged by size: 

                 574     357      86
 
BssSI C'ACGA_G

Cuts at:      0     929    1017 
   Size:        929      88
 
BstYI r'GATC_y

Cuts at:      0     148     389     674     777     876    1017 
   Size:        148     241     285     103      99     141

  Fragments arranged by size: 

                 285     241     148     141     103      99
 
Bsu36I CC'TnA_GG

Cuts at:      0     692    1017 
   Size:        692     325
 
BtgI C'CryG_G

Cuts at:      0     644    1017 
   Size:        644     373
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     747    1017 
   Size:        747     270
 
BtsI GCAGTG_nn'

Cuts at:      0     243    1017 
   Size:        243     774
 
EaeI y'GGCC_r

Cuts at:      0      68    1017 
   Size:         68     949
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     334     961    1017 
   Size:         88     246     627      56

  Fragments arranged by size: 

                 627     246      88      56
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     430     439    1017 
   Size:        430       9     578

  Fragments arranged by size: 

                 578     430       9
 
EcoICRI GAG'CTC

Cuts at:      0     816    1017 
   Size:        816     201
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     580     599     643     832    1017 
   Size:        226     354      19      44     189     185

  Fragments arranged by size: 

                 354     226     189     185      44      19
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     117     972    1017 
   Size:        117     855      45

  Fragments arranged by size: 

                 855     117      45
 
HaeII r_GCGC'y

Cuts at:      0     216     913     954    1017 
   Size:        216     697      41      63

  Fragments arranged by size: 

                 697     216      63      41
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     572     604    1017 
   Size:        572      32     413

  Fragments arranged by size: 

                 572     413      32
 
HincII GTy'rAC

Cuts at:      0     501    1017 
   Size:        501     516
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     875    1017 
   Size:        583     292     142

  Fragments arranged by size: 

                 583     292     142
 
MslI CAynn'nnrTG

Cuts at:      0     130     766    1017 
   Size:        130     636     251

  Fragments arranged by size: 

                 636     251     130
 
MspA1I CmG'CkG

Cuts at:      0      18    1017 
   Size:         18     999
 
NaeI GCC'GGC

Cuts at:      0     445    1017 
   Size:        445     572
 
NgoMIV G'CCGG_C

Cuts at:      0     443    1017 
   Size:        443     574
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     713    1017 
   Size:        713     304
 
PvuII CAG'CTG

Cuts at:      0      18    1017 
   Size:         18     999
 
SacI G_AGCT'C

Cuts at:      0     818    1017 
   Size:        818     199
 
SapI GCTCTTCn'nnn_

Cuts at:      0     334    1017 
   Size:        334     683
 
SfcI C'TryA_G

Cuts at:      0     993    1017 
   Size:        993      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     274     354    1017 
   Size:        274      80     663

  Fragments arranged by size: 

                 663     274      80
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     279    1017 
   Size:        279     738
 
XmnI GAAnn'nnTTC

Cuts at:      0      44    1017 
   Size:         44     973
 
ZraI GAC'GTC

Cuts at:      0     384    1017 
   Size:        384     633
 

 Enzymes that do cut:

     AarI    AatII     AccI     AfeI    AlwNI     ApoI    BamHI     BanI
    BanII     BbsI    BbvCI    BciVI     BglI    BglII     BlpI Bme1580I
     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspEI    BspMI    BsrDI    BsrFI    BssSI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EarI     EciI  EcoICRI
  Eco57MI     FalI    HaeII    Hin4I   HincII     MmeI     MslI   MspA1I
     NaeI   NgoMIV    PflMI    PvuII     SacI     SapI     SfcI    TaqII
     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI     BcgI     BclI    BfrBI    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspHI    BsrBI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI
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