(Circular) (Six-base) MAPSORT of: pFN21AB8457.seq Check: 1878 from: 1 to: 5878 pFN21AB8457 5878 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2797 2797 Size: 5878 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3941 5325 5593 1632 Size: 2309 1384 268 1917 Fragments arranged by size: 2309 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 2184 4777 5754 1203 Size: 981 2593 977 1327 Fragments arranged by size: 2593 1327 981 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2348 2380 2465 2497 2348 Size: 32 85 32 5729 Fragments arranged by size: 5729 85 32 32 AlwNI CAG_nnn'CTG Cuts at: 2768 2789 2831 2897 2964 3621 5193 2768 Size: 21 42 66 67 657 1572 3453 Fragments arranged by size: 3453 1572 657 67 66 42 21 ApaI G_GGCC'C Cuts at: 2616 2891 2616 Size: 275 5603 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 3140 3187 3413 3565 5674 1493 Size: 1647 47 226 152 2109 1697 Fragments arranged by size: 2109 1697 1647 226 152 47 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 2129 2558 2931 3045 3203 1870 Size: 73 186 429 373 114 158 4545 Fragments arranged by size: 4545 429 373 186 158 114 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2284 3082 3199 4654 Size: 325 217 627 243 254 798 117 1455 Cuts at: 4654 618 Size: 1842 Fragments arranged by size: 1842 1455 798 627 325 254 243 217 117 BanII G_rGCy'C Cuts at: 1391 2559 2616 2891 3197 1391 Size: 1168 57 275 306 4072 Fragments arranged by size: 4072 1168 306 275 57 BbeI G_GCGC'C Cuts at: 2034 2288 3086 2034 Size: 254 798 4826 Fragments arranged by size: 4826 798 254 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2167 928 Size: 971 268 4639 Fragments arranged by size: 4639 971 268 BbvCI CC'TCA_GC Cuts at: 2195 2624 2782 2898 3153 2195 Size: 429 158 116 255 4920 Fragments arranged by size: 4920 429 255 158 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4069 4103 1903 Size: 34 50 34 2048 34 3678 Fragments arranged by size: 3678 2048 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 2115 1548 Size: 567 5311 BfrBI ATG'CAT Cuts at: 2152 2152 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 2417 3295 2417 Size: 878 5000 Bme1580I G_kGCm'C Cuts at: 1790 2616 2891 3114 3939 5095 1790 Size: 826 275 223 825 1156 2573 Fragments arranged by size: 2573 1156 826 825 275 223 BmgBI CAC'GTC Cuts at: 1318 2187 1318 Size: 869 5009 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2885 2909 2974 Size: 416 53 83 399 24 1468 24 65 Cuts at: 2974 3101 3609 4567 442 Size: 127 508 958 1753 Fragments arranged by size: 1753 1468 958 508 416 399 127 83 65 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2443 2661 4538 1239 Size: 1204 218 1877 2579 Fragments arranged by size: 2579 1877 1204 218 Bpu10I CC'TnA_GC Cuts at: 2195 2624 2782 2898 3153 2195 Size: 429 158 116 255 4920 Fragments arranged by size: 4920 429 255 158 116 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4003 4868 5166 4003 Size: 865 298 4715 Fragments arranged by size: 4715 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2523 4541 882 Size: 617 1024 2018 2219 Fragments arranged by size: 2219 2018 1024 617 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 5872 5872 Size: 5878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2285 3083 3685 Size: 53 83 186 936 498 254 798 602 Cuts at: 3685 4067 4702 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 798 635 602 498 382 254 186 83 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2348 2378 2441 2471 1178 Size: 30 165 30 945 30 63 30 4585 Fragments arranged by size: 4585 945 165 63 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2612 3170 1186 Size: 1426 558 3894 Fragments arranged by size: 3894 1426 558 BseYI C'CCAG_C Cuts at: 2070 2373 2698 2740 2761 2824 2879 2976 3113 Size: 303 325 42 21 63 55 97 137 Cuts at: 3113 5081 5577 2070 Size: 1968 496 2371 Fragments arranged by size: 2371 1968 496 325 303 137 97 63 55 42 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2298 2470 1163 Size: 573 562 172 4571 Fragments arranged by size: 4571 573 562 172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2225 3939 4024 5095 2225 Size: 1714 85 1071 3008 Fragments arranged by size: 3008 1714 1071 85 BsmI GAATG_Cn' Cuts at: 3389 3482 3389 Size: 93 5785 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2225 2559 2616 2891 3114 3197 3939 Size: 399 435 334 57 275 223 83 742 Cuts at: 3939 4024 5095 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 334 275 223 85 83 57 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 2643 3767 5497 2643 Size: 1124 1730 3024 Fragments arranged by size: 3024 1730 1124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2797 2947 3233 844 Size: 1953 150 286 3489 Fragments arranged by size: 3489 1953 286 150 BsrBI CCG'CTC Cuts at: 1394 1823 2508 2571 3022 3765 1394 Size: 429 685 63 451 743 3507 Fragments arranged by size: 3507 743 685 451 429 63 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 4547 65 Size: 1183 3125 174 1396 Fragments arranged by size: 3125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2332 4522 4709 1700 Size: 110 522 2190 187 2869 Fragments arranged by size: 2869 2190 522 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 2402 3938 4950 2402 Size: 1536 1012 3330 Fragments arranged by size: 3330 1536 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2269 794 Size: 1475 4403 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 2047 2155 2917 2047 Size: 108 762 5008 Fragments arranged by size: 5008 762 108 BstYI r'GATC_y Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873 Size: 1236 367 385 17 1452 86 12 346 Cuts at: 5873 1972 Size: 1977 Fragments arranged by size: 1977 1452 1236 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2161 2203 2445 2718 2802 2851 513 Size: 552 1096 42 242 273 84 49 3540 Fragments arranged by size: 3540 1096 552 273 242 84 49 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2213 487 Size: 683 56 987 4152 Fragments arranged by size: 4152 987 683 56 BtsI GCAGTG_nn' Cuts at: 3394 4188 4208 3394 Size: 794 20 5064 Fragments arranged by size: 5064 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2188 2223 529 Size: 35 1624 35 4184 Fragments arranged by size: 4184 1624 35 35 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2334 2452 Size: 54 1425 50 275 18 420 84 118 Cuts at: 2452 2986 3325 4214 5843 8 Size: 534 339 889 1629 43 Fragments arranged by size: 1629 1425 889 534 420 339 275 118 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3066 3808 1353 Size: 1713 742 3423 Fragments arranged by size: 3423 1713 742 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2129 2582 4465 4839 4985 387 Size: 1064 138 540 453 1883 374 146 1280 Fragments arranged by size: 1883 1280 1064 540 453 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2443 2661 3941 4538 5325 5593 1239 Size: 393 811 218 1280 597 787 268 1524 Fragments arranged by size: 1524 1280 811 787 597 393 268 218 EcoO109I rG'GnC_Cy Cuts at: 2613 2723 2807 2927 2936 3102 2613 Size: 110 84 120 9 166 5389 Fragments arranged by size: 5389 166 120 110 84 9 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2437 2437 Size: 5878 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 2034 2288 2768 3086 5025 1678 Size: 356 254 480 318 1939 2531 Fragments arranged by size: 2531 1939 480 356 318 254 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2212 2244 2290 2322 2441 Size: 32 135 32 640 32 46 32 119 Cuts at: 2441 2473 2891 2923 4519 4551 4593 4625 1373 Size: 32 418 32 1596 32 42 32 2626 Fragments arranged by size: 2626 1596 640 418 135 119 46 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3222 3468 669 Size: 988 281 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KasI G'GCGC_C Cuts at: 2030 2284 3082 2030 Size: 254 798 4826 Fragments arranged by size: 4826 798 254 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4992 5176 1305 Size: 3687 184 2007 Fragments arranged by size: 3687 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 10 Size: 54 1425 343 420 3636 Fragments arranged by size: 3636 1425 420 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2153 3836 4195 4354 518 Size: 648 987 1683 359 159 2042 Fragments arranged by size: 2042 1683 987 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2266 2669 2687 2879 3294 3971 5119 5364 Size: 660 403 18 192 415 677 1148 245 Cuts at: 5364 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 660 415 403 245 192 18 NarI GG'CG_CC Cuts at: 2031 2285 3083 2031 Size: 254 798 4826 Fragments arranged by size: 4826 798 254 NcoI C'CATG_G Cuts at: 513 1065 2851 513 Size: 552 1786 3540 Fragments arranged by size: 3540 1786 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NsiI A_TGCA'T Cuts at: 2154 2154 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 2156 3236 4781 1132 Size: 97 927 1080 1545 2229 Fragments arranged by size: 2229 1545 1080 927 97 PasI CC'CwG_GG Cuts at: 1348 2017 1348 Size: 669 5209 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 2216 2216 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2348 2380 3947 3979 5460 5492 2348 Size: 32 1567 32 1481 32 2734 Fragments arranged by size: 2734 1567 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2723 2807 2927 2936 3102 2723 Size: 84 120 9 166 5499 Fragments arranged by size: 5499 166 120 84 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PspOMI G'GGCC_C Cuts at: 2612 2887 2612 Size: 275 5603 PstI C_TGCA'G Cuts at: 830 2110 2265 2668 3230 830 Size: 1280 155 403 562 3478 Fragments arranged by size: 3478 1280 562 403 155 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 PvuII CAG'CTG Cuts at: 2266 2669 2879 2266 Size: 403 210 5265 Fragments arranged by size: 5265 403 210 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SanDI GG'GwC_CC Cuts at: 2927 2927 Size: 5878 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 2106 2261 2664 3226 4361 5042 5233 Size: 219 1061 155 403 562 1135 681 191 Cuts at: 5233 826 Size: 1471 Fragments arranged by size: 1471 1135 1061 681 562 403 219 191 155 SfoI GGC'GCC Cuts at: 2032 2286 3084 2032 Size: 254 798 4826 Fragments arranged by size: 4826 798 254 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 2560 3047 3205 1872 Size: 688 487 158 4545 Fragments arranged by size: 4545 688 487 158 SmlI C'TyrA_G Cuts at: 820 1017 1943 3982 4883 5145 820 Size: 197 926 2039 901 262 1553 Fragments arranged by size: 2039 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SrfI GCCC'GGGC Cuts at: 2560 3047 2560 Size: 487 5391 SspI AAT'ATT Cuts at: 5 52 3802 5 Size: 47 3750 2081 Fragments arranged by size: 3750 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2608 2851 3317 513 Size: 552 1543 243 466 3074 Fragments arranged by size: 3074 1543 552 466 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2062 2139 2240 2541 3905 3922 4075 Size: 1255 482 77 101 301 1364 17 153 Cuts at: 4075 4260 4705 325 Size: 185 445 1498 Fragments arranged by size: 1498 1364 1255 482 445 301 185 153 101 77 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4124 5836 96 Size: 274 80 33 51 1443 2147 1712 138 Fragments arranged by size: 2147 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3059 3091 5838 5870 3059 Size: 32 2747 32 3067 Fragments arranged by size: 3067 2747 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2859 1316 Size: 1543 4335 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2044 2467 2044 Size: 423 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 2558 3045 3203 1870 Size: 688 487 158 4545 Fragments arranged by size: 4545 688 487 158 XmnI GAAnn'nnTTC Cuts at: 4007 4007 Size: 5878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SwaI