Restriction map for
pFN21AB8455
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AvrII BalI BamHI
BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstNI BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FbaI FokI FseI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeIII MboII MfeI MflI
MluI MluNI MroI MscI MseI
MunI MvaI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 283 GTCTAC GT^[AC][GT]AC
AfaI 108 GTAC GT^AC
Alw44I 118 GTGCAC G^TGCAC
AlwNI 73 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 118 GTGCAC G^TGCAC
AspI 277 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 263 TGCGCA TGC^GCA
BanII 64 GAGCTC G[GA]GC[CT]^C
BbrPI 121 CACGTG CAC^GTG
BpmI 156 CTCCAG CTGGAG(16/14)
BsaAI 121 CACGTG [CT]AC^GT[GA]
BsaBI 6 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 184 CTGCAC GTGCAG(16/14)
BsmFI 127 GTCCC GGGAC(10/14)
BsrBI 219 CCGCTC CCGCTC(-3/-3)
BsrFI 91 ACCGGC [GA]^CCGG[CT]
BsrI 105 CCAGT ACTGG(1/-1)
Cfr10I 91 ACCGGC [GA]^CCGG[CT]
DpnII 201 GATC ^GATC
FauI 32 CCCGC CCCGC(4/6)
FspI 263 TGCGCA TGC^GCA
HaeII 257 AGCGCC [GA]GCGC^[CT]
HphI 68 TCACC GGTGA(8/7)
MamI 6 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 201 GATC ^GATC
MspA1I 76 CAGCTG C[AC]G^C[GT]G
NdeII 201 GATC ^GATC
PflFI 277 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 121 CACGTG CAC^GTG
PmlI 121 CACGTG CAC^GTG
PstI 177 CTGCAG CTGCA^G
PvuII 76 CAGCTG CAG^CTG
RsaI 108 GTAC GT^AC
SacI 64 GAGCTC GAGCT^C
Sau3AI 201 GATC ^GATC
Tth111I 277 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AspHI 64 GAGCTC G[AT]GC[AT]^C
118 GTGCAC
AvaII 126 GGTCC G^G[AT]CC
132 GGACC
BcnI 129 CCCGG CC^[GC]GG
273 CCCGG
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
118 GTGCAC
BssHII 170 GCGCGC G^CGCGC
209 GCGCGC
BssKI 129 CCCGG ^CC[ACGT]GG
273 CCCGG
Cfr13I 126 GGTCC G^G[ACGT]CC
132 GGACC
EaeI 251 TGGCCG [CT]^GGCC[GA]
311 CGGCCA
MaeII 26 ACGT A^CGT
122 ACGT
MnlI 38 CCTC CCTC(7/6)
57 CCTC
MslI 21 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
111 CATCGCGGTG
NciI 129 CCCGG CC^[GC]GG
273 CCCGG
NlaIII 141 CATG CATG^
303 CATG
PflMI 72 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
135 CCAGCTCATGG
Sau96I 126 GGTCC G^G[ACGT]CC
132 GGACC
ScrFI 129 CCCGG CC^[ACGT]GG
273 CCCGG
SfaNI 191 GCATC GCATC(5/9)
266 GCATC
SfcI 177 CTGCAG C^T[GA][CT]AG
225 CTACAG
Van91I 72 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
135 CCAGCTCATGG
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BsiYI BslI Bsp1286I BstUI Cac8I
CfoI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MspI MvnI
MwoI NlaIV TseI