Restriction map for
pFN21AB8455
(Linear) (Six-base) MAPSORT of: KIBB8455.seq Check: 6900 from: 1 to: 345
KIBB8455.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 284 345
Size: 284 61
AleI CACnn'nnGTG
Cuts at: 0 25 345
Size: 25 320
AlwNI CAG_nnn'CTG
Cuts at: 0 78 345
Size: 78 267
ApaLI G'TGCA_C
Cuts at: 0 118 345
Size: 118 227
BanII G_rGCy'C
Cuts at: 0 68 345
Size: 68 277
Bme1580I G_kGCm'C
Cuts at: 0 32 122 345
Size: 32 90 223
Fragments arranged by size:
223 90 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 99 345
Size: 99 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 345
Size: 141 204
BsaAI yAC'GTr
Cuts at: 0 123 345
Size: 123 222
BsaBI GATnn'nnATC
Cuts at: 0 10 345
Size: 10 335
BseYI C'CCAG_C
Cuts at: 0 71 345
Size: 71 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 345
Size: 169 176
BsiHKAI G_wGCw'C
Cuts at: 0 68 122 345
Size: 68 54 223
Fragments arranged by size:
223 68 54
Bsp1286I G_dGCh'C
Cuts at: 0 32 68 122 345
Size: 32 36 54 223
Fragments arranged by size:
223 54 36 32
BsrBI CCG'CTC
Cuts at: 0 221 345
Size: 221 124
BsrFI r'CCGG_y
Cuts at: 0 91 345
Size: 91 254
BssHII G'CGCG_C
Cuts at: 0 170 209 345
Size: 170 39 136
Fragments arranged by size:
170 136 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 96 345
Size: 96 249
EaeI y'GGCC_r
Cuts at: 0 251 311 345
Size: 251 60 34
Fragments arranged by size:
251 60 34
EcoICRI GAG'CTC
Cuts at: 0 66 345
Size: 66 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 345
Size: 141 204
FspI TGC'GCA
Cuts at: 0 265 345
Size: 265 80
HaeII r_GCGC'y
Cuts at: 0 261 345
Size: 261 84
MslI CAynn'nnrTG
Cuts at: 0 25 115 345
Size: 25 90 230
Fragments arranged by size:
230 90 25
MspA1I CmG'CkG
Cuts at: 0 78 345
Size: 78 267
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 141 345
Size: 78 63 204
Fragments arranged by size:
204 78 63
PfoI T'CCnGG_A
Cuts at: 0 128 345
Size: 128 217
PmlI CAC'GTG
Cuts at: 0 123 345
Size: 123 222
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 208 240 345
Size: 208 32 105
Fragments arranged by size:
208 105 32
PstI C_TGCA'G
Cuts at: 0 181 345
Size: 181 164
PvuII CAG'CTG
Cuts at: 0 78 345
Size: 78 267
SacI G_AGCT'C
Cuts at: 0 68 345
Size: 68 277
SfcI C'TryA_G
Cuts at: 0 177 225 345
Size: 177 48 120
Fragments arranged by size:
177 120 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 345
Size: 85 260
TatI w'GTAC_w
Cuts at: 0 107 345
Size: 107 238
Tth111I GACn'n_nGTC
Cuts at: 0 280 345
Size: 280 65
Enzymes that do cut:
AccI AleI AlwNI ApaLI BanII Bme1580I BmrI BpmI
BsaAI BsaBI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI
BssHII BtgZI EaeI EcoICRI Eco57MI FspI HaeII MslI
MspA1I PflMI PfoI PmlI PsrI PstI PvuII SacI
SfcI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI