(Circular) (Six-base) MAPSORT of: pFN21AB8454.seq Check: 6949 from: 1 to: 6304 pFN21AB8454 6304 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2940 4114 278 Size: 53 83 186 936 1404 1174 2468 Fragments arranged by size: 2468 1404 1174 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3647 1937 Size: 1710 4594 Acc65I G'GTAC_C Cuts at: 2454 3625 2454 Size: 1171 5133 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3163 3488 4367 5751 6019 1632 Size: 1531 325 879 1384 268 1917 Fragments arranged by size: 1917 1531 1384 879 325 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 3444 5203 6180 1203 Size: 2241 1759 977 1327 Fragments arranged by size: 2241 1759 1327 977 AgeI A'CCGG_T Cuts at: 3480 5135 3480 Size: 1655 4649 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 2564 1166 Size: 1398 4906 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2390 3161 3254 3465 1184 Size: 1206 771 93 211 4023 Fragments arranged by size: 4023 1206 771 211 93 AlwNI CAG_nnn'CTG Cuts at: 2254 4047 5619 2254 Size: 1793 1572 2939 Fragments arranged by size: 2939 1793 1572 ApaLI G'TGCA_C Cuts at: 4361 5517 4361 Size: 1156 5148 ApoI r'AATT_y Cuts at: 1493 3613 3839 3991 6100 1493 Size: 2120 226 152 2109 1697 Fragments arranged by size: 2120 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4858 160 Size: 872 3826 1606 Fragments arranged by size: 3826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 2109 2189 2306 2392 2469 3278 3557 Size: 73 166 80 117 86 77 809 279 Cuts at: 3557 3576 3629 1870 Size: 19 53 4545 Fragments arranged by size: 4545 809 279 166 117 86 80 77 73 53 19 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2438 2471 2438 Size: 33 6271 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 2454 2492 2602 2614 Size: 325 217 627 579 88 38 110 12 Cuts at: 2614 2783 3074 3543 3625 5080 618 Size: 169 291 469 82 1455 1842 Fragments arranged by size: 1842 1455 627 579 469 325 291 217 169 110 88 82 38 12 BanII G_rGCy'C Cuts at: 1391 2283 3021 3519 3623 1391 Size: 892 738 498 104 4072 Fragments arranged by size: 4072 892 738 498 104 BbeI G_GCGC'C Cuts at: 2496 3547 2496 Size: 1051 5253 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2812 2932 928 Size: 971 913 120 4300 Fragments arranged by size: 4300 971 913 120 BbvCI CC'TCA_GC Cuts at: 2126 2126 Size: 6304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4495 4529 1903 Size: 34 2558 34 3678 Fragments arranged by size: 3678 2558 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 5406 4196 Size: 1210 5094 BclI T'GATC_A Cuts at: 1548 1548 Size: 6304 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2529 2533 4915 Size: 107 122 71 631 605 857 4 2382 Cuts at: 4915 136 Size: 1525 Fragments arranged by size: 2382 1525 857 631 605 122 107 71 4 BglII A'GATC_T Cuts at: 6299 6299 Size: 6304 BlpI GC'TnA_GC Cuts at: 3721 3721 Size: 6304 Bme1580I G_kGCm'C Cuts at: 1790 2559 2607 3077 4365 5521 1790 Size: 769 48 470 1288 1156 2573 Fragments arranged by size: 2573 1288 1156 769 470 48 BmgBI CAC'GTC Cuts at: 1318 2671 2695 1318 Size: 1353 24 4927 Fragments arranged by size: 4927 1353 24 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4035 4993 442 Size: 416 53 83 399 24 2618 958 1753 Fragments arranged by size: 2618 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2399 3101 4964 1239 Size: 1160 702 1863 2579 Fragments arranged by size: 2579 1863 1160 702 Bpu10I CC'TnA_GC Cuts at: 2126 2462 2126 Size: 336 5968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2741 2978 4429 5294 5592 2741 Size: 237 1451 865 298 3453 Fragments arranged by size: 3453 1451 865 298 237 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4967 882 Size: 617 3468 2219 Fragments arranged by size: 3468 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4095 493 Size: 711 2891 2702 Fragments arranged by size: 2891 2702 711 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 2493 2703 2904 Size: 53 83 186 936 816 144 210 201 Cuts at: 2904 2937 2982 3544 4111 4493 5128 275 Size: 33 45 562 567 382 635 1451 Fragments arranged by size: 1451 936 816 635 567 562 382 210 201 186 144 83 53 45 33 BsaWI w'CCGG_w Cuts at: 3480 4737 5135 5409 5556 5967 3480 Size: 1257 398 274 147 411 3817 Fragments arranged by size: 3817 1257 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2504 2534 3074 3104 1178 Size: 30 165 30 1101 30 540 30 4378 Fragments arranged by size: 4378 1101 540 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 2420 2422 1186 Size: 1105 129 2 5068 Fragments arranged by size: 5068 1105 129 2 BseYI C'CCAG_C Cuts at: 2261 2543 2686 3226 3330 3434 5507 6003 2261 Size: 282 143 540 104 104 2073 496 2562 Fragments arranged by size: 2562 2073 540 496 282 143 104 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2057 2588 3082 3224 3236 1163 Size: 573 321 531 494 142 12 4231 Fragments arranged by size: 4231 573 531 494 321 142 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2274 2402 2753 2848 3481 3754 Size: 275 186 273 128 351 95 633 273 Cuts at: 3754 4515 5543 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 633 351 275 273 273 186 128 95 BsiHKAI G_wGCw'C Cuts at: 2283 2814 3444 3527 4365 4450 5521 2283 Size: 531 630 83 838 85 1071 3066 Fragments arranged by size: 3066 1071 838 630 531 85 83 BsmI GAATG_Cn' Cuts at: 3815 3908 3815 Size: 93 6211 BsmBI CGTCTCn'nnnn_ Cuts at: 2008 2911 2008 Size: 903 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2559 2607 2814 3021 3077 3444 Size: 399 493 276 48 207 207 56 367 Cuts at: 3444 3519 3527 3623 4365 4450 5521 1391 Size: 75 8 96 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 493 399 367 276 207 207 96 85 75 56 48 8 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 2589 2670 2886 2931 3471 3501 3659 Size: 1373 372 81 216 45 540 30 158 Cuts at: 3659 844 Size: 3489 Fragments arranged by size: 3489 1373 540 372 216 158 81 45 30 BsrBI CCG'CTC Cuts at: 1394 1823 2596 2755 4191 1394 Size: 429 773 159 1436 3507 Fragments arranged by size: 3507 1436 773 429 159 BsrDI GCAATG_nn' Cuts at: 65 1248 4799 4973 65 Size: 1183 3551 174 1396 Fragments arranged by size: 3551 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2342 2847 3200 3248 3480 4948 Size: 110 238 294 505 353 48 232 1468 Cuts at: 4948 5135 1700 Size: 187 2869 Fragments arranged by size: 2869 1468 505 353 294 238 232 187 110 48 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 2651 2681 3299 4364 5376 2651 Size: 30 618 1065 1012 3579 Fragments arranged by size: 3579 1065 1012 618 30 BstAPI GCAn_nnn'nTGC Cuts at: 794 2254 794 Size: 1460 4844 BstBI TT'CG_AA Cuts at: 5108 5108 Size: 6304 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6304 BstXI CCAn_nnnn'nTGG Cuts at: 2377 2377 Size: 6304 BstYI r'GATC_y Cuts at: 1972 2964 3634 4001 4386 4403 5855 5941 5953 Size: 992 670 367 385 17 1452 86 12 Cuts at: 5953 6299 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 992 670 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2415 2415 Size: 6304 BtgI C'CryG_G Cuts at: 513 1065 2747 3370 513 Size: 552 1682 623 3447 Fragments arranged by size: 3447 1682 623 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5565 Fragments arranged by size: 5565 683 56 BtsI GCAGTG_nn' Cuts at: 3126 3224 3256 3820 4614 4634 3126 Size: 98 32 564 794 20 4796 Fragments arranged by size: 4796 794 564 98 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DraIII CAC_nnn'GTG Cuts at: 2290 2809 2290 Size: 519 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5311 809 Size: 507 3995 1802 Fragments arranged by size: 3995 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2271 2399 Size: 54 1425 50 275 18 216 225 128 Cuts at: 2399 2444 2750 2845 3202 3751 4640 6269 8 Size: 45 306 95 357 549 889 1629 43 Fragments arranged by size: 1629 1425 889 549 357 306 275 225 216 128 95 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2271 2399 2750 2845 3751 1537 Size: 275 459 128 351 95 906 4090 Fragments arranged by size: 4090 906 459 351 275 128 95 EarI CTCTTCn'nnn_ Cuts at: 1353 4234 1353 Size: 2881 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2789 3131 3295 3611 4891 5265 Size: 1064 138 1200 342 164 316 1280 374 Cuts at: 5265 5411 387 Size: 146 1280 Fragments arranged by size: 1280 1280 1200 1064 374 342 316 164 146 138 EcoICRI GAG'CTC Cuts at: 2281 2281 Size: 6304 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 3101 3163 3488 4367 4964 5751 Size: 393 767 702 62 325 879 597 787 Cuts at: 5751 6019 1239 Size: 268 1524 Fragments arranged by size: 1524 879 787 767 702 597 393 325 268 62 EcoNI CCTnn'n_nnAGG Cuts at: 2894 2894 Size: 6304 EcoO109I rG'GnC_Cy Cuts at: 2201 3355 3554 2201 Size: 1154 199 4951 Fragments arranged by size: 4951 1154 199 EcoRI G'AATT_C Cuts at: 3613 3991 3613 Size: 378 5926 FseI GG_CCGG'CC Cuts at: 2052 2052 Size: 6304 FspI TGC'GCA Cuts at: 2333 2882 2918 3038 4810 2333 Size: 549 36 120 1772 3827 Fragments arranged by size: 3827 1772 549 120 36 FspAI rTGC'GCAy Cuts at: 2333 2333 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 2469 2496 3547 5451 1678 Size: 791 27 1051 1904 2531 Fragments arranged by size: 2531 1904 1051 791 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2891 2923 3074 3106 4945 Size: 32 135 32 1319 32 151 32 1839 Cuts at: 4945 4977 5019 5051 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1839 1319 151 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2647 3648 3894 669 Size: 988 281 709 1001 246 3079 Fragments arranged by size: 3079 1001 988 709 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6304 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KasI G'GCGC_C Cuts at: 2492 3543 2492 Size: 1051 5253 KpnI G_GTAC'C Cuts at: 2458 3629 2458 Size: 1171 5133 MfeI C'AATT_G Cuts at: 3903 3903 Size: 6304 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2601 2748 5418 5602 1305 Size: 1296 147 2670 184 2007 Fragments arranged by size: 2670 2007 1296 184 147 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4482 Fragments arranged by size: 4482 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2372 2564 3212 3447 4262 4621 Size: 648 843 363 192 648 235 815 359 Cuts at: 4621 4780 518 Size: 159 2042 Fragments arranged by size: 2042 843 815 648 648 363 359 235 192 159 MspA1I CmG'CkG Cuts at: 1606 2254 2547 2749 3372 3720 4397 5545 5790 Size: 648 293 202 623 348 677 1148 245 Cuts at: 5790 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 648 623 348 293 245 202 NaeI GCC'GGC Cuts at: 2050 2344 2849 3250 2050 Size: 294 505 401 5104 Fragments arranged by size: 5104 505 401 294 NarI GG'CG_CC Cuts at: 2493 3544 2493 Size: 1051 5253 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5752 NdeI CA'TA_TG Cuts at: 387 387 Size: 6304 NgoMIV G'CCGG_C Cuts at: 2048 2342 2847 3248 2048 Size: 294 505 401 5104 Fragments arranged by size: 5104 505 401 294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 2750 3751 2750 Size: 1001 5303 NspI r_CATG'y Cuts at: 1132 1229 3037 3112 3448 3662 5207 1132 Size: 97 1808 75 336 214 1545 2229 Fragments arranged by size: 2229 1808 1545 336 214 97 75 PasI CC'CwG_GG Cuts at: 1348 2607 1348 Size: 1259 5045 PciI A'CATG_T Cuts at: 3444 5203 3444 Size: 1759 4545 PflMI CCAn_nnn'nTGG Cuts at: 1108 2290 2686 3211 1108 Size: 1182 396 525 4201 Fragments arranged by size: 4201 1182 525 396 PfoI T'CCnGG_A Cuts at: 2763 2823 2763 Size: 60 6244 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3355 3554 3355 Size: 199 6105 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PstI C_TGCA'G Cuts at: 830 2250 3117 3430 3656 830 Size: 1420 867 313 226 3478 Fragments arranged by size: 3478 1420 867 313 226 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 PvuII CAG'CTG Cuts at: 2547 2547 Size: 6304 SacI G_AGCT'C Cuts at: 2283 2283 Size: 6304 SacII CC_GC'GG Cuts at: 2750 3373 2750 Size: 623 5681 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SanDI GG'GwC_CC Cuts at: 3554 3554 Size: 6304 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 2573 3161 4552 6264 2573 Size: 588 1391 1712 2613 Fragments arranged by size: 2613 1712 1391 588 SexAI A'CCwGG_T Cuts at: 2136 2136 Size: 6304 SfcI C'TryA_G Cuts at: 826 1045 2246 3113 3426 3652 4787 5468 5659 Size: 219 1201 867 313 226 1135 681 191 Cuts at: 5659 826 Size: 1471 Fragments arranged by size: 1471 1201 1135 867 681 313 226 219 191 SfoI GGC'GCC Cuts at: 2494 3545 2494 Size: 1051 5253 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 2394 2471 3578 3631 1872 Size: 522 77 1107 53 4545 Fragments arranged by size: 4545 1107 522 77 53 SmlI C'TyrA_G Cuts at: 820 1017 1943 2756 2993 4408 5309 5571 820 Size: 197 926 813 237 1415 901 262 1553 Fragments arranged by size: 1553 1415 926 901 813 262 237 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6304 StyI C'CwwG_G Cuts at: 513 1065 2337 2589 3743 513 Size: 552 1272 252 1154 3074 Fragments arranged by size: 3074 1272 1154 552 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2497 2909 3029 4331 4348 4501 4686 Size: 1255 917 412 120 1302 17 153 185 Cuts at: 4686 5131 325 Size: 445 1498 Fragments arranged by size: 1498 1302 1255 917 445 412 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2571 2793 3159 Size: 274 80 33 51 1443 594 222 366 Cuts at: 3159 4550 6262 96 Size: 1391 1712 138 Fragments arranged by size: 1712 1443 1391 594 366 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2504 2536 6264 6296 2504 Size: 32 3728 32 2512 Fragments arranged by size: 3728 2512 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2089 2903 2936 2981 1316 Size: 773 814 33 45 4639 Fragments arranged by size: 4639 814 773 45 33 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2596 2596 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 2392 2469 3576 3629 1870 Size: 522 77 1107 53 4545 Fragments arranged by size: 4545 1107 522 77 53 XmnI GAAnn'nnTTC Cuts at: 4433 4433 Size: 6304 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2938 4112 276 Size: 53 83 186 936 1404 1174 2468 Fragments arranged by size: 2468 1404 1174 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoRV FalI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SapI SfiI SrfI SwaI