Restriction map for
pFN21AB8448
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvrII BamHI BbeI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsgI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eam1105I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MluI MseI
MunI NarI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 1241 TCCGGA T^CCGGA
AflIII 86 ACGTGT A^C[GA][CT]GT
AlwI 933 GATCC GGATC(4/5)
Aor51HI 119 AGCGCT AGC^GCT
Asp700I 354 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1049 CCCGGG C^[CT]CG[GA]G
AviII 626 TGCGCA TGC^GCA
BbsI 964 GTCTTC GAAGAC(2/6)
BcgI 53 GCAACCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 964 GTCTTC GAAGAC(2/6)
BseAI 1241 TCCGGA T^CCGGA
BsiEI 1181 CGGCCG CG[GA][CT]^CG
BsmBI 388 GAGACG CGTCTC(1/5)
BsmFI 719 GGGAC GGGAC(10/14)
BsoBI 1049 CCCGGG C^[CT]CG[GA]G
BspEI 1241 TCCGGA T^CCGGA
BsrFI 1027 GCCGGC [GA]^CCGG[CT]
BssSI 787 CACGAG CACGAG(-5/-1)
BstEII 253 GGTGACC G^GT[ACGT]ACC
BstPI 253 GGTGACC G^GT[ACGT]ACC
BstXI 143 CCACCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 932 AGATCC [GA]^GATC[CT]
Cfr10I 1027 GCCGGC [GA]^CCGG[CT]
DdeI 103 CTGAG C^T[ACGT]AG
DraIII 637 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1181 CGGCCG C^GGCCG
EclXI 1181 CGGCCG C^GGCCG
Eco47III 119 AGCGCT AGC^GCT
Eco52I 1181 CGGCCG C^GGCCG
EcoNI 1313 CCTACCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 253 GGTGACC G^GT[ACGT]ACC
EcoT14I 1220 CCAAGG C^C[AT][AT]GG
FspI 626 TGCGCA TGC^GCA
MflI 932 AGATCC [GA]^GATC[CT]
MroI 1241 TCCGGA T^CCGGA
NaeI 1027 GCCGGC GCC^GGC
NgoMIV 1027 GCCGGC G^CCGGC
NotI 1180 GCGGCCGC GC^GGCCGC
PflMI 636 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 719 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1057 CTGCAG CTGCA^G
PvuII 1172 CAGCTG CAG^CTG
SapI 975 GAAGAGC GCTCTTC(1/4)
SfcI 1057 CTGCAG C^T[GA][CT]AG
SfiI 1182 GGCCGCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1049 CCCGGG CCC^GGG
SphI 1280 GCATGC GCATG^C
StuI 1197 AGGCCT AGG^CCT
StyI 1220 CCAAGG C^C[AT][AT]GG
TspRI 1104 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 636 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 932 AGATCC [GA]^GATC[CT]
XmaCI 1049 CCCGGG C^CCGGG
XmaI 1049 CCCGGG C^CCGGG
XmnI 354 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 90 GTAC GT^AC
699 GTAC
ApaI 291 GGGCCC GGGCC^C
659 GGGCCC
BalI 515 TGGCCA TGG^CCA
923 TGGCCA
BanII 291 GGGCCC G[GA]GC[CT]^C
659 GGGCCC
BsaWI 416 TCCGGT [AT]^CCGG[AT]
1241 TCCGGA
BsmAI 388 GAGAC GTCTC(1/5)
202 GTCTC
BspHI 176 TCATGA T^CATGA
497 TCATGA
BsrI 465 CCAGT ACTGG(1/-1)
1103 CCAGT
DraII 290 GGGGCCC [GA]G^G[ACGT]CC[CT]
719 GGGACCT
EcoO109I 290 GGGGCCC [GA]G^G[ACGT]CC[CT]
719 GGGACCT
HgaI 390 GACGC GACGC(5/10)
482 GCGTC
KspI 188 CCGCGG CCGC^GG
1184 CCGCGG
MaeIII 254 GTGAC ^GT[ACGT]AC
424 GTGAC
MluNI 515 TGGCCA TGG^CCA
923 TGGCCA
MscI 515 TGGCCA TGG^CCA
923 TGGCCA
NspI 170 ACATGC [GA]CATG^[CT]
1280 GCATGC
RcaI 176 TCATGA T^CATGA
497 TCATGA
RsaI 90 GTAC GT^AC
699 GTAC
SacII 188 CCGCGG CCGC^GG
1184 CCGCGG
Tsp45I 254 GTGAC ^GT[GC]AC
424 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI AvaII BanI
BbrPI BbvI BcnI BglI BmyI
BpmI BsaAI BsaJI BseRI BsiYI
BslI Bsp1286I BspMI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII DsaI EaeI EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PmaCI
PmlI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TthHB8I