(Circular) (Six-base) MAPSORT of: pFN21AB8448.seq Check: 3375 from: 1 to: 6040 pFN21AB8448 6040 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2159 2159 Size: 6040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4103 5487 5755 1632 Size: 2471 1384 268 1917 Fragments arranged by size: 2471 1917 1384 268 AfeI AGC'GCT Cuts at: 2126 2126 Size: 6040 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 2091 4939 5916 1203 Size: 888 2848 977 1327 Fragments arranged by size: 2848 1327 977 888 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 2153 1166 Size: 987 5053 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 3783 5355 3783 Size: 1572 4468 ApaI G_GGCC'C Cuts at: 2300 2668 2300 Size: 368 5672 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 3054 3365 1870 Size: 73 1111 311 4545 Fragments arranged by size: 4545 1111 311 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2281 2372 2510 3361 4816 Size: 325 217 627 494 91 138 851 1455 Cuts at: 4816 618 Size: 1842 Fragments arranged by size: 1842 1455 851 627 494 325 217 138 91 BanII G_rGCy'C Cuts at: 1391 2300 2668 3359 1391 Size: 909 368 691 4072 Fragments arranged by size: 4072 909 691 368 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2962 928 Size: 971 1063 4006 Fragments arranged by size: 4006 1063 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2047 2081 4231 4265 1903 Size: 34 50 34 26 34 2150 34 3678 Fragments arranged by size: 3678 2150 50 34 34 34 34 26 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2025 2330 2528 Size: 107 122 71 631 605 353 305 198 Cuts at: 2528 3194 4651 136 Size: 666 1457 1525 Fragments arranged by size: 1525 1457 666 631 605 353 305 198 122 107 71 BglII A'GATC_T Cuts at: 6035 6035 Size: 6040 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2043 2300 2444 2668 4101 5257 1790 Size: 253 257 144 224 1433 1156 2573 Fragments arranged by size: 2573 1433 1156 257 253 224 144 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3102 3771 4729 Size: 416 53 83 399 24 1685 669 958 Cuts at: 4729 442 Size: 1753 Fragments arranged by size: 1753 1685 958 669 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2861 2894 3233 3275 3299 4700 1239 Size: 1046 576 33 339 42 24 1401 2579 Fragments arranged by size: 2579 1401 1046 576 339 42 33 24 Bpu10I CC'TnA_GC Cuts at: 2108 2108 Size: 6040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4165 5030 5328 4165 Size: 865 298 4877 Fragments arranged by size: 4877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4703 882 Size: 617 3204 2219 Fragments arranged by size: 3204 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2038 2356 2659 3831 493 Size: 711 834 318 303 1172 2702 Fragments arranged by size: 2702 1172 834 711 318 303 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3847 4229 4864 275 Size: 53 83 186 936 2314 382 635 1451 Fragments arranged by size: 2314 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2421 3246 4473 4871 5145 5292 5703 2421 Size: 825 1227 398 274 147 411 2758 Fragments arranged by size: 2758 1227 825 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2687 2717 2828 2858 3299 Size: 30 165 30 1284 30 111 30 441 Cuts at: 3299 3329 1178 Size: 30 3889 Fragments arranged by size: 3889 1284 441 165 111 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2450 2849 2990 3173 3182 3185 3245 Size: 1033 231 399 141 183 9 3 60 Cuts at: 3245 1186 Size: 3981 Fragments arranged by size: 3981 1033 399 231 183 141 60 9 3 BseYI C'CCAG_C Cuts at: 2667 5243 5739 2667 Size: 2576 496 2968 Fragments arranged by size: 2968 2576 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5467 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3189 3490 4251 5279 1540 Size: 275 186 1188 301 761 1028 2301 Fragments arranged by size: 2301 1188 1028 761 301 275 186 BsiHKAI G_wGCw'C Cuts at: 4101 4186 5257 4101 Size: 85 1071 4884 Fragments arranged by size: 4884 1071 85 BsmI GAATG_Cn' Cuts at: 3551 3644 3551 Size: 93 5947 BsmBI CGTCTCn'nnnn_ Cuts at: 2387 2387 Size: 6040 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2300 2444 2668 3359 4101 4186 Size: 399 253 257 144 224 691 742 85 Cuts at: 4186 5257 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 691 399 257 253 224 144 85 BspEI T'CCGG_A Cuts at: 3246 5703 3246 Size: 2457 3583 BspHI T'CATG_A Cuts at: 2181 2502 3929 5659 2181 Size: 321 1427 1730 2562 Fragments arranged by size: 2562 1730 1427 321 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2159 2637 2715 3395 844 Size: 1315 478 78 680 3489 Fragments arranged by size: 3489 1315 680 478 78 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4535 4709 65 Size: 1183 3287 174 1396 Fragments arranged by size: 3287 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3032 4684 4871 1700 Size: 110 1222 1652 187 2869 Fragments arranged by size: 2869 1652 1222 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 2792 4100 5112 2792 Size: 1308 1012 3720 Fragments arranged by size: 3720 1308 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2557 2604 3042 794 Size: 1763 47 438 3792 Fragments arranged by size: 3792 1763 438 47 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 2258 1184 Size: 1074 4966 BstXI CCAn_nnnn'nTGG Cuts at: 2155 2155 Size: 6040 BstYI r'GATC_y Cuts at: 1972 2937 3370 3737 4122 4139 5591 5677 5689 Size: 965 433 367 385 17 1452 86 12 Cuts at: 5689 6035 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 965 433 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2193 2254 2892 3189 513 Size: 552 1128 61 638 297 3364 Fragments arranged by size: 3364 1128 638 552 297 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2440 487 Size: 683 56 1214 4087 Fragments arranged by size: 4087 1214 683 56 BtsI GCAGTG_nn' Cuts at: 3556 4350 4370 3556 Size: 794 20 5226 Fragments arranged by size: 5226 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DraIII CAC_nnn'GTG Cuts at: 2647 2647 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2080 2520 Size: 54 1425 50 275 18 196 54 440 Cuts at: 2520 2895 2928 3030 3186 3195 3487 4376 6005 Size: 375 33 102 156 9 292 889 1629 Cuts at: 6005 8 Size: 43 Fragments arranged by size: 1629 1425 889 440 375 292 275 196 156 102 54 54 50 43 33 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3186 3487 1537 Size: 275 1374 301 4090 Fragments arranged by size: 4090 1374 301 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2975 3150 3970 1353 Size: 884 738 175 820 3423 Fragments arranged by size: 3423 884 820 738 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2062 2150 2331 4627 5001 5147 Size: 1064 138 473 88 181 2296 374 146 Cuts at: 5147 387 Size: 1280 Fragments arranged by size: 2296 1280 1064 473 374 181 146 138 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2861 2894 3233 3275 3299 4103 Size: 393 653 576 33 339 42 24 804 Cuts at: 4103 4700 5487 5755 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 804 787 653 597 576 393 339 268 42 33 24 EcoNI CCTnn'n_nnAGG Cuts at: 3322 3322 Size: 6040 EcoO109I rG'GnC_Cy Cuts at: 2296 2725 2296 Size: 429 5611 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 2633 4546 2633 Size: 1913 4127 HaeII r_GCGC'y Cuts at: 1678 2056 2128 2173 5187 1678 Size: 378 72 45 3014 2531 Fragments arranged by size: 3014 2531 378 72 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2828 2860 3299 3331 4681 Size: 32 135 32 1256 32 439 32 1350 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 1256 439 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2826 5154 5338 1305 Size: 1521 2328 184 2007 Fragments arranged by size: 2328 2007 1521 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2522 2930 10 Size: 54 1425 343 690 408 3120 Fragments arranged by size: 3120 1425 690 408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2153 2180 2354 2459 2516 2585 2897 Size: 648 987 27 174 105 57 69 312 Cuts at: 2897 3248 3998 4357 4516 518 Size: 351 750 359 159 2042 Fragments arranged by size: 2042 987 750 648 359 351 312 174 159 105 69 57 27 MspA1I CmG'CkG Cuts at: 1606 2195 2446 2722 3179 3191 3304 3456 4133 Size: 589 251 276 457 12 113 152 677 Cuts at: 4133 5281 5526 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 589 457 276 251 245 152 113 12 NaeI GCC'GGC Cuts at: 3034 3034 Size: 6040 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5488 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NgoMIV G'CCGG_C Cuts at: 3032 3032 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3186 3487 3186 Size: 301 5739 NspI r_CATG'y Cuts at: 1132 1229 2179 3289 3398 4943 1132 Size: 97 950 1110 109 1545 2229 Fragments arranged by size: 2229 1545 1110 950 109 97 PasI CC'CwG_GG Cuts at: 1348 2276 1348 Size: 928 5112 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 1108 Size: 1539 4501 PfoI T'CCnGG_A Cuts at: 2871 2871 Size: 6040 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PmlI CAC'GTG Cuts at: 2038 2356 2659 2038 Size: 318 303 5419 Fragments arranged by size: 5419 318 303 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 2725 Size: 6040 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PspOMI G'GGCC_C Cuts at: 2296 2664 2296 Size: 368 5672 PstI C_TGCA'G Cuts at: 830 3066 3392 830 Size: 2236 326 3478 Fragments arranged by size: 3478 2236 326 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 3179 3179 Size: 6040 SacII CC_GC'GG Cuts at: 2196 3192 2196 Size: 996 5044 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SapI GCTCTTCn'nnn_ Cuts at: 2975 2975 Size: 6040 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 3062 3388 4523 5204 5395 826 Size: 219 2017 326 1135 681 191 1471 Fragments arranged by size: 2017 1471 1135 681 326 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3194 3194 Size: 6040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3056 3367 1872 Size: 1184 311 4545 Fragments arranged by size: 4545 1184 311 SmlI C'TyrA_G Cuts at: 820 1017 1943 4144 5045 5307 820 Size: 197 926 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3289 3398 1132 Size: 2157 109 3774 Fragments arranged by size: 3774 2157 109 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 3204 1517 Size: 1687 4353 StyI C'CwwG_G Cuts at: 513 1065 3225 3479 513 Size: 552 2160 254 3074 Fragments arranged by size: 3074 2160 552 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2360 2921 4067 4084 4237 4422 4867 Size: 1255 780 561 1146 17 153 185 445 Cuts at: 4867 325 Size: 1498 Fragments arranged by size: 1498 1255 1146 780 561 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 4286 5998 Size: 274 80 33 51 1443 117 2192 1712 Cuts at: 5998 96 Size: 138 Fragments arranged by size: 2192 1712 1443 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3054 3365 1870 Size: 1184 311 4545 Fragments arranged by size: 4545 1184 311 XmnI GAAnn'nnTTC Cuts at: 2363 4169 2363 Size: 1806 4234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SanDI SexAI SfoI SrfI SwaI XcmI