Restriction map for
pFN21AB8448
(Linear) (Six-base) MAPSORT of: KIBB8448.seq Check: 9739 from: 1 to: 1335
KIBB8448.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 154 1335
Size: 154 1181
AfeI AGC'GCT
Cuts at: 0 121 1335
Size: 121 1214
AflIII A'CryG_T
Cuts at: 0 86 1335
Size: 86 1249
AleI CACnn'nnGTG
Cuts at: 0 148 1335
Size: 148 1187
ApaI G_GGCC'C
Cuts at: 0 295 663 1335
Size: 295 368 672
Fragments arranged by size:
672 368 295
AvaI C'yCGr_G
Cuts at: 0 1049 1335
Size: 1049 286
BanI G'GyrC_C
Cuts at: 0 276 367 505 1335
Size: 276 91 138 830
Fragments arranged by size:
830 276 138 91
BanII G_rGCy'C
Cuts at: 0 295 663 1335
Size: 295 368 672
Fragments arranged by size:
672 368 295
BbsI GAAGACnn'nnnn_
Cuts at: 0 957 1335
Size: 957 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 42 76 1335
Size: 42 34 1259
Fragments arranged by size:
1259 42 34
BglI GCCn_nnn'nGGC
Cuts at: 0 20 325 523 1189 1335
Size: 20 305 198 666 146
Fragments arranged by size:
666 305 198 146 20
Bme1580I G_kGCm'C
Cuts at: 0 38 295 439 663 1335
Size: 38 257 144 224 672
Fragments arranged by size:
672 257 224 144 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1097 1335
Size: 1097 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 856 889 1228 1270 1294 1335
Size: 280 576 33 339 42 24 41
Fragments arranged by size:
576 339 280 42 41 33 24
Bpu10I CC'TnA_GC
Cuts at: 0 103 1335
Size: 103 1232
BsaAI yAC'GTr
Cuts at: 0 33 351 654 1335
Size: 33 318 303 681
Fragments arranged by size:
681 318 303 33
BsaWI w'CCGG_w
Cuts at: 0 416 1241 1335
Size: 416 825 94
Fragments arranged by size:
825 416 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 682 712 823 853 1294 1324 1335
Size: 682 30 111 30 441 30 11
Fragments arranged by size:
682 441 111 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 445 844 985 1168 1177 1180 1240
Size: 214 231 399 141 183 9 3 60
Cuts at: 1240 1335
Size: 95
Fragments arranged by size:
399 231 214 183 141 95 60 9
3
BseYI C'CCAG_C
Cuts at: 0 662 1335
Size: 662 673
BsiEI CG_ry'CG
Cuts at: 0 1184 1335
Size: 1184 151
BsmBI CGTCTCn'nnnn_
Cuts at: 0 382 1335
Size: 382 953
Bsp1286I G_dGCh'C
Cuts at: 0 38 295 439 663 1335
Size: 38 257 144 224 672
Fragments arranged by size:
672 257 224 144 38
BspEI T'CCGG_A
Cuts at: 0 1241 1335
Size: 1241 94
BspHI T'CATG_A
Cuts at: 0 176 497 1335
Size: 176 321 838
Fragments arranged by size:
838 321 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 154 632 710 1335
Size: 154 478 78 625
Fragments arranged by size:
625 478 154 78
BsrFI r'CCGG_y
Cuts at: 0 1027 1335
Size: 1027 308
BssSI C'ACGA_G
Cuts at: 0 787 1335
Size: 787 548
BstAPI GCAn_nnn'nTGC
Cuts at: 0 552 599 1037 1335
Size: 552 47 438 298
Fragments arranged by size:
552 438 298 47
BstEII G'GTnAC_C
Cuts at: 0 253 1335
Size: 253 1082
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 1335
Size: 150 1185
BstYI r'GATC_y
Cuts at: 0 932 1335
Size: 932 403
BtgI C'CryG_G
Cuts at: 0 188 249 887 1184 1335
Size: 188 61 638 297 151
Fragments arranged by size:
638 297 188 151 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 435 1335
Size: 435 900
DraIII CAC_nnn'GTG
Cuts at: 0 642 1335
Size: 642 693
EaeI y'GGCC_r
Cuts at: 0 21 75 515 890 923 1025 1181 1190
Size: 21 54 440 375 33 102 156 9
Cuts at: 1190 1335
Size: 145
Fragments arranged by size:
440 375 156 145 102 54 33 21
9
EagI C'GGCC_G
Cuts at: 0 1181 1335
Size: 1181 154
EarI CTCTTCn'nnn_
Cuts at: 0 232 970 1145 1335
Size: 232 738 175 190
Fragments arranged by size:
738 232 190 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 57 145 326 1335
Size: 57 88 181 1009
Fragments arranged by size:
1009 181 88 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 856 889 1228 1270 1294 1335
Size: 280 576 33 339 42 24 41
Fragments arranged by size:
576 339 280 42 41 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1317 1335
Size: 1317 18
EcoO109I rG'GnC_Cy
Cuts at: 0 291 720 1335
Size: 291 429 615
Fragments arranged by size:
615 429 291
FspI TGC'GCA
Cuts at: 0 628 1335
Size: 628 707
HaeII r_GCGC'y
Cuts at: 0 51 123 168 1335
Size: 51 72 45 1167
Fragments arranged by size:
1167 72 51 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 1294 1326 1335
Size: 823 32 439 32 9
Fragments arranged by size:
823 439 32 32 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1335
Size: 821 514
MscI TGG'CCA
Cuts at: 0 517 925 1335
Size: 517 408 410
Fragments arranged by size:
517 410 408
MslI CAynn'nnrTG
Cuts at: 0 148 175 349 454 511 580 892 1243
Size: 148 27 174 105 57 69 312 351
Cuts at: 1243 1335
Size: 92
Fragments arranged by size:
351 312 174 148 105 92 69 57
27
MspA1I CmG'CkG
Cuts at: 0 190 441 717 1174 1186 1299 1335
Size: 190 251 276 457 12 113 36
Fragments arranged by size:
457 276 251 190 113 36 12
NaeI GCC'GGC
Cuts at: 0 1029 1335
Size: 1029 306
NgoMIV G'CCGG_C
Cuts at: 0 1027 1335
Size: 1027 308
NotI GC'GGCC_GC
Cuts at: 0 1181 1335
Size: 1181 154
NspI r_CATG'y
Cuts at: 0 174 1284 1335
Size: 174 1110 51
Fragments arranged by size:
1110 174 51
PasI CC'CwG_GG
Cuts at: 0 271 1335
Size: 271 1064
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1335
Size: 642 693
PfoI T'CCnGG_A
Cuts at: 0 866 1335
Size: 866 469
PmlI CAC'GTG
Cuts at: 0 33 351 654 1335
Size: 33 318 303 681
Fragments arranged by size:
681 318 303 33
PpuMI rG'GwC_Cy
Cuts at: 0 720 1335
Size: 720 615
PspOMI G'GGCC_C
Cuts at: 0 291 659 1335
Size: 291 368 676
Fragments arranged by size:
676 368 291
PstI C_TGCA'G
Cuts at: 0 1061 1335
Size: 1061 274
PvuII CAG'CTG
Cuts at: 0 1174 1335
Size: 1174 161
SacII CC_GC'GG
Cuts at: 0 191 1187 1335
Size: 191 996 148
Fragments arranged by size:
996 191 148
SapI GCTCTTCn'nnn_
Cuts at: 0 970 1335
Size: 970 365
SfcI C'TryA_G
Cuts at: 0 1057 1335
Size: 1057 278
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1189 1335
Size: 1189 146
SmaI CCC'GGG
Cuts at: 0 1051 1335
Size: 1051 284
SphI G_CATG'C
Cuts at: 0 1284 1335
Size: 1284 51
StuI AGG'CCT
Cuts at: 0 1199 1335
Size: 1199 136
StyI C'CwwG_G
Cuts at: 0 1220 1335
Size: 1220 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 355 916 1335
Size: 355 561 419
Fragments arranged by size:
561 419 355
TatI w'GTAC_w
Cuts at: 0 89 1335
Size: 89 1246
XmaI C'CCGG_G
Cuts at: 0 1049 1335
Size: 1049 286
XmnI GAAnn'nnTTC
Cuts at: 0 358 1335
Size: 358 977
Enzymes that do cut:
AarI AfeI AflIII AleI ApaI AvaI BanI BanII
BbsI BcgI BglI Bme1580I BmrI BpmI Bpu10I BsaAI
BsaWI BsaXI BseRI BseYI BsiEI BsmBI Bsp1286I BspEI
BspHI BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NgoMIV NotI NspI PasI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacII SapI SfcI
SfiI SmaI SphI StuI StyI TaqII TatI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsgI BsiHKAI BsiWI BsmI BsrBI BsrDI BsrGI
BssHII BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DrdI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NruI NsiI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XhoI ZraI