Restriction map for
pFN21AB8447
(Circular) (Six-base) MAPSORT of: pFN21AB8447.seq Check: 6047 from: 1 to: 6112
pFN21AB8447 6112 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3455 1937
Size: 1518 4594
Acc65I G'GTAC_C
Cuts at: 2974 3433 2974
Size: 459 5653
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2894 4175 5559 5827 1632
Size: 492 770 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 770 492 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 2149 2318 5011 5988 1203
Size: 946 169 2693 977 1327
Fragments arranged by size:
2693 1327 977 946 169
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 2857 3855 5427 2857
Size: 998 1572 3542
Fragments arranged by size:
3542 1572 998
ApaI G_GGCC'C
Cuts at: 3236 3236
Size: 6112
ApaLI G'TGCA_C
Cuts at: 2015 4169 5325 2015
Size: 2154 1156 2802
Fragments arranged by size:
2802 2154 1156
ApoI r'AATT_y
Cuts at: 1493 2084 2118 2286 2563 2806 3421 3647 3799
Size: 591 34 168 277 243 615 226 152
Cuts at: 3799 5908 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 615 591 277 243 226 168
152 34
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 3437 1870
Size: 73 1494 4545
Fragments arranged by size:
4545 1494 73
AvrII C'CTAG_G
Cuts at: 3088 3088
Size: 6112
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 3433 4888 618
Size: 325 217 627 1187 459 1455 1842
Fragments arranged by size:
1842 1455 1187 627 459 325 217
BanII G_rGCy'C
Cuts at: 1391 2612 3236 3431 1391
Size: 1221 624 195 4072
Fragments arranged by size:
4072 1221 624 195
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2672 928
Size: 971 773 4368
Fragments arranged by size:
4368 971 773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3159 4723 136
Size: 107 122 71 631 605 1487 1564 1525
Fragments arranged by size:
1564 1525 1487 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 2019 3236 4173 5329 1790
Size: 229 1217 937 1156 2573
Fragments arranged by size:
2573 1217 1156 937 229
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2717 3115 3843
Size: 416 53 83 399 24 1300 398 728
Cuts at: 3843 4801 442
Size: 958 1753
Fragments arranged by size:
1753 1300 958 728 416 399 398 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4772 1239
Size: 3533 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2704 4237 5102 5400 2429
Size: 275 1533 865 298 3141
Fragments arranged by size:
3141 1533 865 298 275
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4775 882
Size: 617 3276 2219
Fragments arranged by size:
3276 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2729 3381 3919 4301
Size: 53 83 186 936 1196 652 538 382
Cuts at: 4301 4936 275
Size: 635 1451
Fragments arranged by size:
1451 1196 936 652 635 538 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4545 4943 5217 5364 5775 4545
Size: 398 274 147 411 4882
Fragments arranged by size:
4882 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5887
Fragments arranged by size:
5887 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2395 1186
Size: 1209 4903
BseYI C'CCAG_C
Cuts at: 3395 5315 5811 3395
Size: 1920 496 3696
Fragments arranged by size:
3696 1920 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2490 3104 1163
Size: 573 754 614 4171
Fragments arranged by size:
4171 754 614 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 2612 4173 4258 5329 2019
Size: 593 1561 85 1071 2802
Fragments arranged by size:
2802 1561 1071 593 85
BsiWI C'GTAC_G
Cuts at: 3279 3279
Size: 6112
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
BsmBI CGTCTCn'nnnn_
Cuts at: 2615 2615
Size: 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2612 3236 3431 4173 4258 5329
Size: 399 229 593 624 195 742 85 1071
Cuts at: 5329 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 624 593 399 229 195
85
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 2254 4001 5731 2254
Size: 1747 1730 2635
Fragments arranged by size:
2635 1747 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3467 844
Size: 2623 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3000 4607 4781 65
Size: 1183 1752 1607 174 1396
Fragments arranged by size:
1752 1607 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3110 4756 4943 1700
Size: 110 1300 1646 187 2869
Fragments arranged by size:
2869 1646 1300 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 4172 5184 4172
Size: 1012 5100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6112
BstBI TT'CG_AA
Cuts at: 2556 2743 4916 2556
Size: 187 2173 3752
Fragments arranged by size:
3752 2173 187
BstEII G'GTnAC_C
Cuts at: 1184 2479 1184
Size: 1295 4817
BstYI r'GATC_y
Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107
Size: 1470 367 385 17 1452 86 12 346
Cuts at: 6107 1972
Size: 1977
Fragments arranged by size:
1977 1470 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3299 3299
Size: 6112
BtgI C'CryG_G
Cuts at: 513 1065 2004 2406 2540 2787 3040 3345 513
Size: 552 939 402 134 247 253 305 3280
Fragments arranged by size:
3280 939 552 402 305 253 247 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3298 487
Size: 683 56 2072 3301
Fragments arranged by size:
3301 2072 683 56
BtsI GCAGTG_nn'
Cuts at: 2612 3628 4422 4442 2612
Size: 1016 794 20 4282
Fragments arranged by size:
4282 1016 794 20
ClaI AT'CG_AT
Cuts at: 1554 2796 1554
Size: 1242 4870
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3146 3559 4448
Size: 54 1425 50 275 18 1316 413 889
Cuts at: 4448 6077 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1316 889 413 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2043 4042 1353
Size: 690 1999 3423
Fragments arranged by size:
3423 1999 690
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2979 4699 5073 5219 387
Size: 1064 138 1390 1720 374 146 1280
Fragments arranged by size:
1720 1390 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2610 2610
Size: 6112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2894 4175 4772 5559 5827 1239
Size: 393 492 770 1281 597 787 268 1524
Fragments arranged by size:
1524 1281 787 770 597 492 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2234 2234
Size: 6112
EcoO109I rG'GnC_Cy
Cuts at: 3026 3232 3233 3026
Size: 206 1 5905
Fragments arranged by size:
5905 206 1
EcoRI G'AATT_C
Cuts at: 2806 3421 3799 2806
Size: 615 378 5119
Fragments arranged by size:
5119 615 378
EcoRV GAT'ATC
Cuts at: 2188 2188
Size: 6112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 6112
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 5259 1678
Size: 3581 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2978 3010 4753 4785 4827
Size: 32 135 32 1406 32 1743 32 42
Cuts at: 4827 4859 1373
Size: 32 2626
Fragments arranged by size:
2626 1743 1406 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2848 3456 3702 669
Size: 988 281 910 608 246 3079
Fragments arranged by size:
3079 988 910 608 281 246
HindIII A'AGCT_T
Cuts at: 748 2447 748
Size: 1699 4413
HpaI GTT'AAC
Cuts at: 2848 3702 2848
Size: 854 5258
KpnI G_GTAC'C
Cuts at: 2978 3437 2978
Size: 459 5653
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2392 3303 5226 5410 1305
Size: 1087 911 1923 184 2007
Fragments arranged by size:
2007 1923 1087 911 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4290
Fragments arranged by size:
4290 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2160 4070 4429 4588 518
Size: 648 994 1910 359 159 2042
Fragments arranged by size:
2042 1910 994 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2642 3042 3273 3528 4205 5353 5598 1606
Size: 1036 400 231 255 677 1148 245 2120
Fragments arranged by size:
2120 1148 1036 677 400 255 245 231
NaeI GCC'GGC
Cuts at: 3112 3112
Size: 6112
NcoI C'CATG_G
Cuts at: 513 1065 2004 2406 2787 3345 513
Size: 552 939 402 381 558 3280
Fragments arranged by size:
3280 939 558 552 402 381
NdeI CA'TA_TG
Cuts at: 387 2660 387
Size: 2273 3839
NgoMIV G'CCGG_C
Cuts at: 3110 3110
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2153 2322 3055 3470 5015 1132
Size: 97 924 169 733 415 1545 2229
Fragments arranged by size:
2229 1545 924 733 415 169 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 2149 2318 5011 2149
Size: 169 2693 3250
Fragments arranged by size:
3250 2693 169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2048 2857 1108
Size: 940 809 4363
Fragments arranged by size:
4363 940 809
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3026 3026
Size: 6112
PshAI GACnn'nnGTC
Cuts at: 1613 2319 1613
Size: 706 5406
PsiI TTA'TAA
Cuts at: 2400 3682 2400
Size: 1282 4830
PspOMI G'GGCC_C
Cuts at: 3232 3232
Size: 6112
PstI C_TGCA'G
Cuts at: 830 3464 830
Size: 2634 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2642 2642
Size: 6112
SacI G_AGCT'C
Cuts at: 2612 2612
Size: 6112
SacII CC_GC'GG
Cuts at: 3043 3043
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SanDI GG'GwC_CC
Cuts at: 3026 3026
Size: 6112
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 3460 4595 5276 5467 826
Size: 219 2415 1135 681 191 1471
Fragments arranged by size:
2415 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 2719 4216 5117 5379 820
Size: 197 926 501 275 1497 901 262 1553
Fragments arranged by size:
1553 1497 926 901 501 275 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3055 3470 1132
Size: 1923 415 3774
Fragments arranged by size:
3774 1923 415
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 2603 2787 2822 3019 3088
Size: 552 939 402 197 184 35 197 69
Cuts at: 3088 3345 3551 513
Size: 257 206 3074
Fragments arranged by size:
3074 939 552 402 257 206 197 197
184 69 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4139 4156 4309 4494 4939 325
Size: 1255 2559 17 153 185 445 1498
Fragments arranged by size:
2559 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2646 3352 4358
Size: 274 80 33 51 1443 669 706 1006
Cuts at: 4358 6070 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1006 706 669 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6072 6104 6072
Size: 32 6080
Tth111I GACn'n_nGTC
Cuts at: 1316 2728 1316
Size: 1412 4700
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3161 3392 3161
Size: 231 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BstXI BstZ17I DraIII FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SapI SexAI SfiI SfoI SrfI SwaI