(Circular) (Six-base) MAPSORT of: pFN21AB8433.seq Check: 5499 from: 1 to: 5554 pFN21AB8433 5554 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2218 2218 Size: 5554 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3364 278 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2897 1937 Size: 960 4594 Acc65I G'GTAC_C Cuts at: 2400 2875 2400 Size: 475 5079 AclI AA'CG_TT Cuts at: 1563 3681 4054 1563 Size: 2118 373 3063 Fragments arranged by size: 3063 2118 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2303 2666 3617 5001 5269 1632 Size: 482 189 363 951 1384 268 1917 Fragments arranged by size: 1917 1384 951 482 363 268 189 AfeI AGC'GCT Cuts at: 2843 2843 Size: 5554 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5357 AflIII A'CryG_T Cuts at: 1203 2454 4453 5430 1203 Size: 1251 1999 977 1327 Fragments arranged by size: 1999 1327 1251 977 AgeI A'CCGG_T Cuts at: 4385 4385 Size: 5554 AhdI GACnn_n'nnGTC Cuts at: 4283 4283 Size: 5554 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5554 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2240 1184 Size: 1056 4498 AlwNI CAG_nnn'CTG Cuts at: 2161 2251 2554 3297 4869 2161 Size: 90 303 743 1572 2846 Fragments arranged by size: 2846 1572 743 303 90 ApaLI G'TGCA_C Cuts at: 3611 4767 3611 Size: 1156 4398 ApoI r'AATT_y Cuts at: 1493 2863 3089 3241 5350 1493 Size: 1370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4108 160 Size: 872 3076 1606 Fragments arranged by size: 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 AvaI C'yCGr_G Cuts at: 1870 1943 2346 2879 1870 Size: 73 403 533 4545 Fragments arranged by size: 4545 533 403 73 BamHI G'GATC_C Cuts at: 2884 3251 2884 Size: 367 5187 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2400 2875 4330 618 Size: 325 217 627 613 475 1455 1842 Fragments arranged by size: 1842 1455 627 613 475 325 217 BanII G_rGCy'C Cuts at: 1391 2410 2735 2775 2873 1391 Size: 1019 325 40 98 4072 Fragments arranged by size: 4072 1019 325 98 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2330 928 Size: 971 431 4152 Fragments arranged by size: 4152 971 431 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3745 3779 1903 Size: 34 1808 34 3678 Fragments arranged by size: 3678 1808 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4656 3446 Size: 1210 4344 BclI T'GATC_A Cuts at: 1548 1548 Size: 5554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4165 136 Size: 107 122 71 631 605 2493 1525 Fragments arranged by size: 2493 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5549 5549 Size: 5554 BlpI GC'TnA_GC Cuts at: 2971 2971 Size: 5554 Bme1580I G_kGCm'C Cuts at: 1790 2561 3615 4771 1790 Size: 771 1054 1156 2573 Fragments arranged by size: 2573 1156 1054 771 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5554 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2357 2559 3285 Size: 416 53 83 399 24 940 202 726 Cuts at: 3285 4243 442 Size: 958 1753 Fragments arranged by size: 1753 958 940 726 416 399 202 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2195 2228 2747 4214 1239 Size: 902 54 33 519 1467 2579 Fragments arranged by size: 2579 1467 902 519 54 33 Bpu10I CC'TnA_GC Cuts at: 2205 2205 Size: 5554 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3679 4544 4842 3679 Size: 865 298 4391 Fragments arranged by size: 4391 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4217 882 Size: 617 2718 2219 Fragments arranged by size: 2718 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3345 493 Size: 711 2141 2702 Fragments arranged by size: 2702 2141 711 BsaBI GATnn'nnATC Cuts at: 5548 5548 Size: 5554 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3361 3743 4378 275 Size: 53 83 186 936 1828 382 635 1451 Fragments arranged by size: 1828 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2622 3987 4385 4659 4806 5217 2622 Size: 1365 398 274 147 411 2959 Fragments arranged by size: 2959 1365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5329 Fragments arranged by size: 5329 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5554 BseYI C'CCAG_C Cuts at: 2554 2815 4757 5253 2554 Size: 261 1942 496 2855 Fragments arranged by size: 2855 1942 496 261 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2567 2675 1163 Size: 573 501 330 108 4042 Fragments arranged by size: 4042 573 501 330 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2715 3004 3765 4793 1540 Size: 275 186 714 289 761 1028 2301 Fragments arranged by size: 2301 1028 761 714 289 275 186 BsiHKAI G_wGCw'C Cuts at: 2235 2735 2775 3615 3700 4771 2235 Size: 500 40 840 85 1071 3018 Fragments arranged by size: 3018 1071 840 500 85 40 BsmI GAATG_Cn' Cuts at: 3065 3158 3065 Size: 93 5461 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2410 2561 2735 2775 2873 3615 Size: 399 445 175 151 174 40 98 742 Cuts at: 3615 3700 4771 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 445 399 175 174 151 98 85 40 BspEI T'CCGG_A Cuts at: 2622 5217 2622 Size: 2595 2959 BspHI T'CATG_A Cuts at: 2301 3443 5173 2301 Size: 1142 1730 2682 Fragments arranged by size: 2682 1730 1142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2218 2909 844 Size: 1374 691 3489 Fragments arranged by size: 3489 1374 691 BsrBI CCG'CTC Cuts at: 1394 1823 3441 1394 Size: 429 1618 3507 Fragments arranged by size: 3507 1618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4049 4223 65 Size: 1183 2801 174 1396 Fragments arranged by size: 2801 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4198 4385 1700 Size: 110 2388 187 2869 Fragments arranged by size: 2869 2388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5554 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5554 BssSI C'ACGA_G Cuts at: 3614 4626 3614 Size: 1012 4542 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5554 BstBI TT'CG_AA Cuts at: 4358 4358 Size: 5554 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5554 BstXI CCAn_nnnn'nTGG Cuts at: 2173 2173 Size: 5554 BstYI r'GATC_y Cuts at: 1972 2523 2884 3251 3636 3653 5105 5191 5203 Size: 551 361 367 385 17 1452 86 12 Cuts at: 5203 5549 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 551 385 367 361 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2660 513 Size: 552 939 656 3407 Fragments arranged by size: 3407 939 656 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4815 Fragments arranged by size: 4815 683 56 BtsI GCAGTG_nn' Cuts at: 3070 3864 3884 3070 Size: 794 20 4740 Fragments arranged by size: 4740 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5554 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5519 DraI TTT'AAA Cuts at: 2858 2858 Size: 5554 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4561 809 Size: 507 3245 1802 Fragments arranged by size: 3245 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2478 2579 Size: 54 1425 50 275 18 333 315 101 Cuts at: 2579 2608 2712 3001 3890 5519 8 Size: 29 104 289 889 1629 43 Fragments arranged by size: 1629 1425 889 333 315 289 275 104 101 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2712 3001 1537 Size: 275 900 289 4090 Fragments arranged by size: 4090 900 289 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 3484 1353 Size: 1155 976 3423 Fragments arranged by size: 3423 1155 976 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2054 4141 4515 4661 387 Size: 1064 138 465 2087 374 146 1280 Fragments arranged by size: 2087 1280 1064 465 374 146 138 EcoICRI GAG'CTC Cuts at: 2733 2773 2733 Size: 40 5514 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2141 2195 2228 2303 2666 2747 Size: 393 482 27 54 33 75 363 81 Cuts at: 2747 3617 4214 5001 5269 1239 Size: 870 597 787 268 1524 Fragments arranged by size: 1524 870 787 597 482 393 363 268 81 75 54 33 27 EcoO109I rG'GnC_Cy Cuts at: 2158 2239 2278 2158 Size: 81 39 5434 Fragments arranged by size: 5434 81 39 EcoRI G'AATT_C Cuts at: 2863 3241 2863 Size: 378 5176 FspI TGC'GCA Cuts at: 4060 4060 Size: 5554 HaeII r_GCGC'y Cuts at: 1678 2845 4701 1678 Size: 1167 1856 2531 Fragments arranged by size: 2531 1856 1167 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4195 4227 4269 4301 1373 Size: 32 135 32 2623 32 42 32 2626 Fragments arranged by size: 2626 2623 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2898 3144 669 Size: 988 281 960 246 3079 Fragments arranged by size: 3079 988 960 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5554 HpaI GTT'AAC Cuts at: 3144 3144 Size: 5554 KpnI G_GTAC'C Cuts at: 2404 2879 2404 Size: 475 5079 MfeI C'AATT_G Cuts at: 3153 3153 Size: 5554 MluI A'CGCG_T Cuts at: 5430 5430 Size: 5554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2316 2649 4668 4852 1305 Size: 1011 333 2019 184 2007 Fragments arranged by size: 2019 2007 1011 333 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2165 10 Size: 54 1425 343 333 3399 Fragments arranged by size: 3399 1425 343 333 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 3512 3871 4030 518 Size: 648 1287 1059 359 159 2042 Fragments arranged by size: 2042 1287 1059 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2330 2551 2970 3647 4795 5040 1606 Size: 724 221 419 677 1148 245 2120 Fragments arranged by size: 2120 1148 724 677 419 245 221 NcoI C'CATG_G Cuts at: 513 1065 2004 2660 513 Size: 552 939 656 3407 Fragments arranged by size: 3407 939 656 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5554 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5554 NotI GC'GGCC_GC Cuts at: 3001 3001 Size: 5554 NspI r_CATG'y Cuts at: 1132 1229 2458 2912 4457 1132 Size: 97 1229 454 1545 2229 Fragments arranged by size: 2229 1545 1229 454 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5554 PciI A'CATG_T Cuts at: 2454 4453 2454 Size: 1999 3555 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5554 PfoI T'CCnGG_A Cuts at: 2172 2172 Size: 5554 PmeI GTTT'AAAC Cuts at: 2858 2858 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3623 3655 5136 5168 3623 Size: 32 1481 32 4009 Fragments arranged by size: 4009 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2158 2278 2158 Size: 120 5434 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5554 PsiI TTA'TAA Cuts at: 3124 3124 Size: 5554 PstI C_TGCA'G Cuts at: 830 2229 2906 830 Size: 1399 677 3478 Fragments arranged by size: 3478 1399 677 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5554 PvuII CAG'CTG Cuts at: 2551 2551 Size: 5554 SacI G_AGCT'C Cuts at: 2735 2775 2735 Size: 40 5514 SalI G'TCGA_C Cuts at: 1936 2896 1936 Size: 960 4594 SbfI CC_TGCA'GG Cuts at: 2906 2906 Size: 5554 ScaI AGT'ACT Cuts at: 3802 5514 3802 Size: 1712 3842 SfcI C'TryA_G Cuts at: 826 1045 2225 2902 4037 4718 4909 826 Size: 219 1180 677 1135 681 191 1471 Fragments arranged by size: 1471 1180 1135 681 677 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5554 SmaI CCC'GGG Cuts at: 1872 2348 2881 1872 Size: 476 533 4545 Fragments arranged by size: 4545 533 476 SmlI C'TyrA_G Cuts at: 820 1017 1943 3658 4559 4821 820 Size: 197 926 1715 901 262 1553 Fragments arranged by size: 1715 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3345 493 Size: 2852 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5554 SphI G_CATG'C Cuts at: 1132 2912 1132 Size: 1780 3774 SrfI GCCC'GGGC Cuts at: 2348 2348 Size: 5554 SspI AAT'ATT Cuts at: 5 52 3478 5 Size: 47 3426 2081 Fragments arranged by size: 3426 2081 47 StuI AGG'CCT Cuts at: 1517 2500 1517 Size: 983 4571 StyI C'CwwG_G Cuts at: 513 1065 2004 2382 2660 2993 513 Size: 552 939 378 278 333 3074 Fragments arranged by size: 3074 939 552 378 333 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 3581 3598 3751 3936 4381 325 Size: 1255 741 1260 17 153 185 445 1498 Fragments arranged by size: 1498 1260 1255 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2613 3800 5512 Size: 274 80 33 51 1443 636 1187 1712 Cuts at: 5512 96 Size: 138 Fragments arranged by size: 1712 1443 1187 636 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5514 5546 5514 Size: 32 5522 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5554 XbaI T'CTAG_A Cuts at: 2890 2890 Size: 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2599 2599 Size: 5554 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5554 XmaI C'CCGG_G Cuts at: 1870 2346 2879 1870 Size: 476 533 4545 Fragments arranged by size: 4545 533 476 XmnI GAAnn'nnTTC Cuts at: 3683 3683 Size: 5554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3362 276 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI