Restriction map for
pFN21AB8432
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AgeI ApoI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaHI BsiWI BsrBI BsrDI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I CelII CpoI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccIII 626 TCCGGA T^CCGGA
Asp700I 1920 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 522 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 232 GGATCC G^GATCC
BcgI 750 CGAGACAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2576 AGATCT A^GATCT
BsaBI 1074 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 626 TCCGGA T^CCGGA
BsiEI 111 CGACCG CG[GA][CT]^CG
BsmI 1357 GCATTC GAATGC(1/-1)
Bsp1407I 737 TGTACA T^GTACA
BspDI 844 ATCGAT AT^CGAT
BspEI 626 TCCGGA T^CCGGA
BspLU11I 740 ACATGT A^CATGT
BsrGI 737 TGTACA T^GTACA
BstAPI 831 GCACGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1576 TTCGAA TT^CGAA
ClaI 844 ATCGAT AT^CGAT
DraI 1204 TTTAAA TTT^AAA
DraIII 832 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2290 GACCGGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 22 CTCTTC CTCTTC(1/4)
Ksp632I 22 CTCTTC CTCTTC(1/4)
MamI 1074 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2605 ACGCGT A^CGCGT
MroI 626 TCCGGA T^CCGGA
NspV 1576 TTCGAA TT^CGAA
PaeR7I 748 CTCGAG C^TCGAG
PflFI 522 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 2705 ACCTGGT A^CC[AT]GGT
SfuI 1576 TTCGAA TT^CGAA
SspBI 737 TGTACA T^GTACA
Tth111I 522 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 748 CTCGAG C^TCGAG
XmnI 1920 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1752 GTCTAC GT^[AC][GT]AC
2029 GTCTAC
AccII 1466 CGCG CG^CG
2606 CGCG
AflIII 740 ACATGT A^C[GA][CT]GT
2605 ACGCGT
Alw44I 160 GTGCAC G^TGCAC
1800 GTGCAC
AocI 454 CCTGAGG CC^T[ACGT]AGG
1102 CCTGAGG
Aor51HI 632 AGCGCT AGC^GCT
1682 AGCGCT
ApaLI 160 GTGCAC G^TGCAC
1800 GTGCAC
BfaI 2731 CTAG C^TAG
2785 CTAG
BsaAI 2032 TACGTG [CT]AC^GT[GA]
2632 TACGTG
BsaI 677 GAGACC GGTCTC(1/5)
2504 GAGACC
BseRI 1431 CTCCTC GAGGAG(10/8)
1069 GAGGAG
BspHI 1323 TCATGA T^CATGA
2399 TCATGA
BstUI 1466 CGCG CG^CG
2606 CGCG
Bsu36I 454 CCTGAGG CC^T[ACGT]AGG
1102 CCTGAGG
Eco47III 632 AGCGCT AGC^GCT
1682 AGCGCT
Eco81I 454 CCTGAGG CC^T[ACGT]AGG
1102 CCTGAGG
EcoNI 87 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
851 CCTCCCACAGG
HgaI 1119 GCGTC GACGC(5/10)
1290 GCGTC
MaeI 2731 CTAG C^TAG
2785 CTAG
MseI 1205 TTAA T^TAA
2162 TTAA
MvnI 1466 CGCG CG^CG
2606 CGCG
NspI 471 ACATGC [GA]CATG^[CT]
740 ACATGT
RcaI 1323 TCATGA T^CATGA
2399 TCATGA
SmaI 443 CCCGGG CCC^GGG
1655 CCCGGG
Tru9I 1205 TTAA T^TAA
2162 TTAA
XcmI 645 CCACCGTGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
701 CCAAGAGGTGGCTGG
XmaCI 443 CCCGGG C^CCGGG
1655 CCCGGG
XmaI 443 CCCGGG C^CCGGG
1655 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AhdI AluI AlwI
AlwNI ApaI AspEI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII