(Circular) (Six-base) MAPSORT of: pFN21AB8432.seq Check: 383 from: 1 to: 7504 pFN21AB8432 7504 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2678 2678 Size: 7504 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5314 278 Size: 53 83 186 936 3778 2468 Fragments arranged by size: 3778 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3758 4035 4847 1937 Size: 1821 277 812 4594 Fragments arranged by size: 4594 1821 812 277 Acc65I G'GTAC_C Cuts at: 4825 4825 Size: 7504 AclI AA'CG_TT Cuts at: 1563 5631 6004 1563 Size: 4068 373 3063 Fragments arranged by size: 4068 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2135 3140 4022 5567 6951 7219 1632 Size: 482 21 1005 882 1545 1384 268 1917 Fragments arranged by size: 1917 1545 1384 1005 882 482 268 21 AfeI AGC'GCT Cuts at: 2639 3689 2639 Size: 1050 6454 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7307 AflIII A'CryG_T Cuts at: 1203 2745 4610 6403 7380 1203 Size: 1542 1865 1793 977 1327 Fragments arranged by size: 1865 1793 1542 1327 977 AgeI A'CCGG_T Cuts at: 6335 6335 Size: 7504 AhdI GACnn_n'nnGTC Cuts at: 2357 3841 4134 6233 2357 Size: 1484 293 2099 3628 Fragments arranged by size: 3628 2099 1484 293 AleI CACnn'nnGTG Cuts at: 1166 2905 1166 Size: 1739 5765 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7504 AlwNI CAG_nnn'CTG Cuts at: 2158 2332 2459 5247 6819 2158 Size: 174 127 2788 1572 2843 Fragments arranged by size: 2843 2788 1572 174 127 ApaI G_GGCC'C Cuts at: 2914 2942 3325 2914 Size: 28 383 7093 Fragments arranged by size: 7093 383 28 ApaLI G'TGCA_C Cuts at: 2165 3805 5561 6717 2165 Size: 1640 1756 1156 2952 Fragments arranged by size: 2952 1756 1640 1156 ApoI r'AATT_y Cuts at: 1493 4813 5039 5191 7300 1493 Size: 3320 226 152 2109 1697 Fragments arranged by size: 3320 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6058 160 Size: 872 5026 1606 Fragments arranged by size: 5026 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7504 AvaI C'yCGr_G Cuts at: 1870 1943 2412 2448 2753 3660 4829 1870 Size: 73 469 36 305 907 1169 4545 Fragments arranged by size: 4545 1169 907 469 305 73 36 BamHI G'GATC_C Cuts at: 2237 4834 5201 2237 Size: 2597 367 4540 Fragments arranged by size: 4540 2597 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 2762 4242 4825 6280 Size: 325 217 627 429 546 1480 583 1455 Cuts at: 6280 618 Size: 1842 Fragments arranged by size: 1842 1480 1455 627 583 546 429 325 217 BanII G_rGCy'C Cuts at: 1391 2468 2914 2942 3070 3325 4260 4653 4692 Size: 1077 446 28 128 255 935 393 39 Cuts at: 4692 4823 1391 Size: 131 4072 Fragments arranged by size: 4072 1077 935 446 393 255 131 128 39 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6533 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2744 2778 5695 5729 1903 Size: 34 807 34 2917 34 3678 Fragments arranged by size: 3678 2917 807 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5396 6606 5396 Size: 1210 6294 BclI T'GATC_A Cuts at: 1548 1548 Size: 7504 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 6115 136 Size: 107 122 71 631 605 337 4106 1525 Fragments arranged by size: 4106 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 4581 7499 4581 Size: 2918 4586 BlpI GC'TnA_GC Cuts at: 4921 4921 Size: 7504 Bme1580I G_kGCm'C Cuts at: 1790 2169 2219 2914 2942 3325 3809 4245 5565 Size: 379 50 695 28 383 484 436 1320 Cuts at: 5565 6721 1790 Size: 1156 2573 Fragments arranged by size: 2573 1320 1156 695 484 436 383 379 50 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7504 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2046 3568 5235 Size: 416 53 83 399 24 629 1522 1667 Cuts at: 5235 6193 442 Size: 958 1753 Fragments arranged by size: 1753 1667 1522 958 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7504 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2455 2455 Size: 7504 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2038 2048 2391 2522 2639 3166 3878 6164 Size: 799 10 343 131 117 527 712 2286 Cuts at: 6164 1239 Size: 2579 Fragments arranged by size: 2579 2286 799 712 527 343 131 117 10 Bpu10I CC'TnA_GC Cuts at: 2641 2641 Size: 7504 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2861 3054 3149 4462 4712 5629 6494 6792 2861 Size: 193 95 1313 250 917 865 298 3573 Fragments arranged by size: 3573 1313 917 865 298 250 193 95 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2676 4503 6167 882 Size: 617 1177 1827 1664 2219 Fragments arranged by size: 2219 1827 1664 1177 617 BsaAI yAC'GTr Cuts at: 493 1204 4039 4639 5295 493 Size: 711 2835 600 656 2702 Fragments arranged by size: 2835 2702 711 656 600 BsaBI GATnn'nnATC Cuts at: 3083 7498 3083 Size: 4415 3089 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5311 5693 6328 275 Size: 53 83 186 936 3778 382 635 1451 Fragments arranged by size: 3778 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2118 2631 4296 4332 5937 6335 6609 6756 7167 Size: 513 1665 36 1605 398 274 147 411 Cuts at: 7167 2118 Size: 2455 Fragments arranged by size: 2455 1665 1605 513 411 398 274 147 36 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7279 Fragments arranged by size: 7279 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3089 3427 1186 Size: 1903 338 5263 Fragments arranged by size: 5263 1903 338 BseYI C'CCAG_C Cuts at: 2110 3396 3456 3523 4234 4342 4762 6707 7203 Size: 1286 60 67 711 108 420 1945 496 Cuts at: 7203 2110 Size: 2411 Fragments arranged by size: 2411 1945 1286 711 496 420 108 67 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2239 2902 2948 3257 3392 3584 3869 Size: 573 503 663 46 309 135 192 285 Cuts at: 3869 4097 1163 Size: 228 4570 Fragments arranged by size: 4570 663 573 503 309 285 228 192 135 46 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2119 4954 5715 6743 1540 Size: 275 186 118 2835 761 1028 2301 Fragments arranged by size: 2835 2301 1028 761 275 186 118 BsiHKAI G_wGCw'C Cuts at: 2169 2825 3070 3809 4260 4284 4692 5565 5650 Size: 656 245 739 451 24 408 873 85 Cuts at: 5650 6721 2169 Size: 1071 2952 Fragments arranged by size: 2952 1071 873 739 656 451 408 245 85 24 BsmI GAATG_Cn' Cuts at: 3362 5015 5108 3362 Size: 1653 93 5758 Fragments arranged by size: 5758 1653 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3596 4255 4382 3596 Size: 659 127 6718 Fragments arranged by size: 6718 659 127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2219 2468 2825 2914 2942 3070 Size: 399 379 50 249 357 89 28 128 Cuts at: 3070 3325 3809 4245 4260 4284 4653 4692 4823 Size: 255 484 436 15 24 369 39 131 Cuts at: 4823 5565 5650 6721 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 436 399 379 369 357 255 249 131 128 89 85 50 39 28 24 15 BspEI T'CCGG_A Cuts at: 2631 7167 2631 Size: 4536 2968 BspHI T'CATG_A Cuts at: 3328 4404 5393 7123 3328 Size: 1076 989 1730 3709 Fragments arranged by size: 3709 1730 1076 989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2261 2444 2678 3020 3414 3753 3891 4422 Size: 1417 183 234 342 394 339 138 531 Cuts at: 4422 4859 844 Size: 437 3489 Fragments arranged by size: 3489 1417 531 437 394 342 339 234 183 138 BsrBI CCG'CTC Cuts at: 1394 1823 5391 1394 Size: 429 3568 3507 Fragments arranged by size: 3568 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5999 6173 65 Size: 1183 4751 174 1396 Fragments arranged by size: 4751 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2892 2967 3573 3655 6148 6335 1700 Size: 110 1082 75 606 82 2493 187 2869 Fragments arranged by size: 2869 2493 1082 606 187 110 82 75 BsrGI T'GTAC_A Cuts at: 96 2742 96 Size: 2646 4858 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7504 BssSI C'ACGA_G Cuts at: 5564 6576 5564 Size: 1012 6492 BstAPI GCAn_nnn'nTGC Cuts at: 794 2842 794 Size: 2048 5456 BstBI TT'CG_AA Cuts at: 3582 6308 3582 Size: 2726 4778 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7504 BstYI r'GATC_y Cuts at: 1972 2237 4501 4581 4834 5201 5586 5603 7055 Size: 265 2264 80 253 367 385 17 1452 Cuts at: 7055 7141 7153 7499 1972 Size: 86 12 346 1977 Fragments arranged by size: 2264 1977 1452 385 367 346 265 253 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 2460 3108 2460 Size: 648 6856 BtgI C'CryG_G Cuts at: 513 1065 3620 3646 4080 4176 4644 4659 513 Size: 552 2555 26 434 96 468 15 3358 Fragments arranged by size: 3358 2555 552 468 434 96 26 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3993 4145 4270 487 Size: 683 56 2767 152 125 3721 Fragments arranged by size: 3721 2767 683 152 125 56 BtsI GCAGTG_nn' Cuts at: 3809 4456 5020 5814 5834 3809 Size: 647 564 794 20 5479 Fragments arranged by size: 5479 794 647 564 20 ClaI AT'CG_AT Cuts at: 1554 2850 1554 Size: 1296 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7469 DraI TTT'AAA Cuts at: 3211 4808 3211 Size: 1597 5907 DraIII CAC_nnn'GTG Cuts at: 2842 2842 Size: 7504 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4301 6511 809 Size: 507 2985 2210 1802 Fragments arranged by size: 2985 2210 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2647 2677 Size: 54 1425 50 275 18 306 511 30 Cuts at: 2677 3391 3768 4365 4490 4641 4656 4951 5840 Size: 714 377 597 125 151 15 295 889 Cuts at: 5840 7469 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 714 597 511 377 306 295 275 151 125 54 50 43 30 18 15 EagI C'GGCC_G Cuts at: 1537 1812 4951 1537 Size: 275 3139 4090 Fragments arranged by size: 4090 3139 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 5434 1353 Size: 680 3401 3423 Fragments arranged by size: 3423 3401 680 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3191 3970 6091 6465 6611 387 Size: 1064 138 1602 779 2121 374 146 1280 Fragments arranged by size: 2121 1602 1280 1064 779 374 146 138 EcoICRI GAG'CTC Cuts at: 3068 4258 4690 3068 Size: 1190 432 5882 Fragments arranged by size: 5882 1190 432 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2048 2114 2135 2391 2522 2639 Size: 393 406 10 66 21 256 131 117 Cuts at: 2639 3140 3166 3878 4022 5567 6164 6951 7219 Size: 501 26 712 144 1545 597 787 268 Cuts at: 7219 1239 Size: 1524 Fragments arranged by size: 1545 1524 787 712 597 501 406 393 268 256 144 131 117 66 26 21 10 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2860 2096 Size: 764 6740 EcoO109I rG'GnC_Cy Cuts at: 2107 2191 2341 2456 2865 2938 3000 3664 4128 Size: 84 150 115 409 73 62 664 464 Cuts at: 4128 2107 Size: 5483 Fragments arranged by size: 5483 664 464 409 150 115 84 73 62 EcoRI G'AATT_C Cuts at: 4813 5191 4813 Size: 378 7126 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2749 4477 2749 Size: 1728 5776 FspI TGC'GCA Cuts at: 6010 6010 Size: 7504 HaeII r_GCGC'y Cuts at: 1678 2641 3263 3691 6651 1678 Size: 963 622 428 2960 2531 Fragments arranged by size: 2960 2531 963 622 428 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2343 2375 2425 Size: 32 135 32 566 32 173 32 50 Cuts at: 2425 2457 3595 3627 3827 3859 4120 4152 4500 Size: 32 1138 32 200 32 261 32 348 Cuts at: 4500 4532 4730 4762 6145 6177 6219 6251 1373 Size: 32 198 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1383 1138 566 348 261 200 198 173 135 50 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4848 5094 669 Size: 988 281 2910 246 3079 Fragments arranged by size: 3079 2910 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7504 HpaI GTT'AAC Cuts at: 5094 5094 Size: 7504 KpnI G_GTAC'C Cuts at: 4829 4829 Size: 7504 MfeI C'AATT_G Cuts at: 5103 5103 Size: 7504 MluI A'CGCG_T Cuts at: 4610 7380 4610 Size: 2770 4734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6618 6802 1305 Size: 5313 184 2007 Fragments arranged by size: 5313 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2138 2649 4658 10 Size: 54 1425 343 306 511 2009 2856 Fragments arranged by size: 2856 2009 1425 511 343 306 54 MslI CAynn'nnrTG Cuts at: 518 1166 2905 2970 3138 3287 5462 5821 5980 Size: 648 1739 65 168 149 2175 359 159 Cuts at: 5980 518 Size: 2042 Fragments arranged by size: 2175 2042 1739 648 359 168 159 149 65 MspA1I CmG'CkG Cuts at: 1606 2235 2584 2962 3396 3449 3568 3840 4590 Size: 629 349 378 434 53 119 272 750 Cuts at: 4590 4762 4920 5597 6745 6990 1606 Size: 172 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 750 677 629 434 378 349 272 245 172 158 119 53 NcoI C'CATG_G Cuts at: 513 1065 3620 3646 4176 513 Size: 552 2555 26 530 3841 Fragments arranged by size: 3841 2555 552 530 26 NdeI CA'TA_TG Cuts at: 387 387 Size: 7504 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7504 NotI GC'GGCC_GC Cuts at: 4951 4951 Size: 7504 NspI r_CATG'y Cuts at: 1132 1229 2480 2749 4862 6407 1132 Size: 97 1251 269 2113 1545 2229 Fragments arranged by size: 2229 2113 1545 1251 269 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7504 PciI A'CATG_T Cuts at: 2745 6403 2745 Size: 3658 3846 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 2712 3558 1108 Size: 1104 500 846 5054 Fragments arranged by size: 5054 1104 846 500 PfoI T'CCnGG_A Cuts at: 2294 2568 2595 3793 4629 2294 Size: 274 27 1198 836 5169 Fragments arranged by size: 5169 1198 836 274 27 PmeI GTTT'AAAC Cuts at: 4808 4808 Size: 7504 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2242 2274 3914 3946 4386 4418 5573 5605 7086 Size: 32 1640 32 440 32 1155 32 1481 Cuts at: 7086 7118 2242 Size: 32 2628 Fragments arranged by size: 2628 1640 1481 1155 440 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 2191 2341 2456 2865 3000 3664 4128 2107 Size: 84 150 115 409 135 664 464 5483 Fragments arranged by size: 5483 664 464 409 150 135 115 84 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7504 PsiI TTA'TAA Cuts at: 5074 5074 Size: 7504 PspOMI G'GGCC_C Cuts at: 2910 2938 3321 2910 Size: 28 383 7093 Fragments arranged by size: 7093 383 28 PstI C_TGCA'G Cuts at: 830 4856 830 Size: 4026 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7504 PvuII CAG'CTG Cuts at: 2584 2962 3449 3568 2584 Size: 378 487 119 6520 Fragments arranged by size: 6520 487 378 119 SacI G_AGCT'C Cuts at: 3070 4260 4692 3070 Size: 1190 432 5882 Fragments arranged by size: 5882 1190 432 SalI G'TCGA_C Cuts at: 1936 4846 1936 Size: 2910 4594 SanDI GG'GwC_CC Cuts at: 2191 2191 Size: 7504 SbfI CC_TGCA'GG Cuts at: 4856 4856 Size: 7504 ScaI AGT'ACT Cuts at: 5752 7464 5752 Size: 1712 5792 SexAI A'CCwGG_T Cuts at: 4710 4710 Size: 7504 SfcI C'TryA_G Cuts at: 826 1045 2957 3247 3535 4852 5987 6668 6859 Size: 219 1912 290 288 1317 1135 681 191 Cuts at: 6859 826 Size: 1471 Fragments arranged by size: 1912 1471 1317 1135 681 290 288 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7504 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7504 SmaI CCC'GGG Cuts at: 1872 2450 3662 4831 1872 Size: 578 1212 1169 4545 Fragments arranged by size: 4545 1212 1169 578 SmlI C'TyrA_G Cuts at: 820 1017 1943 2753 2876 3069 3164 4441 4727 Size: 197 926 810 123 193 95 1277 286 Cuts at: 4727 5608 6509 6771 820 Size: 881 901 262 1553 Fragments arranged by size: 1553 1277 926 901 881 810 286 262 197 193 123 95 SnaBI TAC'GTA Cuts at: 493 5295 493 Size: 4802 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7504 SphI G_CATG'C Cuts at: 1132 4862 1132 Size: 3730 3774 SspI AAT'ATT Cuts at: 5 52 5428 5 Size: 47 5376 2081 Fragments arranged by size: 5376 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7504 StyI C'CwwG_G Cuts at: 513 1065 2172 2241 3003 3228 3427 3552 3620 Size: 552 1107 69 762 225 199 125 68 Cuts at: 3620 3646 3711 4176 4653 4674 4943 513 Size: 26 65 465 477 21 269 3074 Fragments arranged by size: 3074 1107 762 552 477 465 269 225 199 125 69 68 65 26 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3315 3550 4671 4709 5531 5548 5701 Size: 1255 1735 235 1121 38 822 17 153 Cuts at: 5701 5886 6331 325 Size: 185 445 1498 Fragments arranged by size: 1735 1498 1255 1121 822 445 235 185 153 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 3720 5750 Size: 274 80 33 51 1443 765 978 2030 Cuts at: 5750 7462 96 Size: 1712 138 Fragments arranged by size: 2030 1712 1443 978 765 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2175 2207 2229 2261 4651 4683 7464 7496 2175 Size: 32 22 32 2390 32 2781 32 2183 Fragments arranged by size: 2781 2390 2183 32 32 32 32 22 Tth111I GACn'n_nGTC Cuts at: 1316 2530 1316 Size: 1214 6290 XbaI T'CTAG_A Cuts at: 4840 4840 Size: 7504 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2657 2713 2657 Size: 56 7448 XhoI C'TCGA_G Cuts at: 1943 2753 1943 Size: 810 6694 XmaI C'CCGG_G Cuts at: 1870 2448 3660 4829 1870 Size: 578 1212 1169 4545 Fragments arranged by size: 4545 1212 1169 578 XmnI GAAnn'nnTTC Cuts at: 3929 5633 3929 Size: 1704 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5312 276 Size: 53 83 186 936 3778 2468 Fragments arranged by size: 3778 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI