Restriction map for
pFN21AB8432
(Linear) (Six-base) MAPSORT of: KIBB8432.seq Check: 2827 from: 1 to: 2799
KIBB8432.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 673 2799
Size: 673 2126
AccI GT'mk_AC
Cuts at: 0 1753 2030 2799
Size: 1753 277 769
Fragments arranged by size:
1753 769 277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 130 1135 2017 2799
Size: 109 21 1005 882 782
Fragments arranged by size:
1005 882 782 109 21
AfeI AGC'GCT
Cuts at: 0 634 1684 2799
Size: 634 1050 1115
Fragments arranged by size:
1115 1050 634
AflIII A'CryG_T
Cuts at: 0 740 2605 2799
Size: 740 1865 194
Fragments arranged by size:
1865 740 194
AhdI GACnn_n'nnGTC
Cuts at: 0 352 1836 2129 2799
Size: 352 1484 293 670
Fragments arranged by size:
1484 670 352 293
AleI CACnn'nnGTG
Cuts at: 0 900 2799
Size: 900 1899
AlwNI CAG_nnn'CTG
Cuts at: 0 153 327 454 2799
Size: 153 174 127 2345
Fragments arranged by size:
2345 174 153 127
ApaI G_GGCC'C
Cuts at: 0 909 937 1320 2799
Size: 909 28 383 1479
Fragments arranged by size:
1479 909 383 28
ApaLI G'TGCA_C
Cuts at: 0 160 1800 2799
Size: 160 1640 999
Fragments arranged by size:
1640 999 160
AvaI C'yCGr_G
Cuts at: 0 407 443 748 1655 2799
Size: 407 36 305 907 1144
Fragments arranged by size:
1144 907 407 305 36
BamHI G'GATC_C
Cuts at: 0 232 2799
Size: 232 2567
BanI G'GyrC_C
Cuts at: 0 211 757 2237 2799
Size: 211 546 1480 562
Fragments arranged by size:
1480 562 546 211
BanII G_rGCy'C
Cuts at: 0 463 909 937 1065 1320 2255 2648 2687
Size: 463 446 28 128 255 935 393 39
Cuts at: 2687 2799
Size: 112
Fragments arranged by size:
935 463 446 393 255 128 112 39
28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 739 773 2799
Size: 739 34 2026
Fragments arranged by size:
2026 739 34
BglII A'GATC_T
Cuts at: 0 2576 2799
Size: 2576 223
Bme1580I G_kGCm'C
Cuts at: 0 164 214 909 937 1320 1804 2240 2799
Size: 164 50 695 28 383 484 436 559
Fragments arranged by size:
695 559 484 436 383 164 50 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 41 1563 2799
Size: 41 1522 1236
Fragments arranged by size:
1522 1236 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 450 2799
Size: 450 2349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 43 386 517 634 1161 1873 2799
Size: 33 10 343 131 117 527 712 926
Fragments arranged by size:
926 712 527 343 131 117 33 10
Bpu10I CC'TnA_GC
Cuts at: 0 636 2799
Size: 636 2163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1049 1144 2457 2707 2799
Size: 856 193 95 1313 250 92
Fragments arranged by size:
1313 856 250 193 95 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 671 2498 2799
Size: 671 1827 301
Fragments arranged by size:
1827 671 301
BsaAI yAC'GTr
Cuts at: 0 2034 2634 2799
Size: 2034 600 165
Fragments arranged by size:
2034 600 165
BsaBI GATnn'nnATC
Cuts at: 0 1078 2799
Size: 1078 1721
BsaWI w'CCGG_w
Cuts at: 0 113 626 2291 2327 2799
Size: 113 513 1665 36 472
Fragments arranged by size:
1665 513 472 113 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1084 1422 2799
Size: 1084 338 1377
Fragments arranged by size:
1377 1084 338
BseYI C'CCAG_C
Cuts at: 0 105 1391 1451 1518 2229 2337 2757 2799
Size: 105 1286 60 67 711 108 420 42
Fragments arranged by size:
1286 711 420 108 105 67 60 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 897 943 1252 1387 1579 1864 2092
Size: 234 663 46 309 135 192 285 228
Cuts at: 2092 2799
Size: 707
Fragments arranged by size:
707 663 309 285 234 228 192 135
46
BsiEI CG_ry'CG
Cuts at: 0 114 2799
Size: 114 2685
BsiHKAI G_wGCw'C
Cuts at: 0 164 820 1065 1804 2255 2279 2687 2799
Size: 164 656 245 739 451 24 408 112
Fragments arranged by size:
739 656 451 408 245 164 112 24
BsmI GAATG_Cn'
Cuts at: 0 1357 2799
Size: 1357 1442
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1591 2250 2377 2799
Size: 1591 659 127 422
Fragments arranged by size:
1591 659 422 127
Bsp1286I G_dGCh'C
Cuts at: 0 164 214 463 820 909 937 1065 1320
Size: 164 50 249 357 89 28 128 255
Cuts at: 1320 1804 2240 2255 2279 2648 2687 2799
Size: 484 436 15 24 369 39 112
Fragments arranged by size:
484 436 369 357 255 249 164 128
112 89 50 39 28 24 15
BspEI T'CCGG_A
Cuts at: 0 626 2799
Size: 626 2173
BspHI T'CATG_A
Cuts at: 0 1323 2399 2799
Size: 1323 1076 400
Fragments arranged by size:
1323 1076 400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 256 439 673 1015 1409 1748 1886 2417
Size: 256 183 234 342 394 339 138 531
Cuts at: 2417 2799
Size: 382
Fragments arranged by size:
531 394 382 342 339 256 234 183
138
BsrFI r'CCGG_y
Cuts at: 0 887 962 1568 1650 2799
Size: 887 75 606 82 1149
Fragments arranged by size:
1149 887 606 82 75
BsrGI T'GTAC_A
Cuts at: 0 737 2799
Size: 737 2062
BstAPI GCAn_nnn'nTGC
Cuts at: 0 837 2799
Size: 837 1962
BstBI TT'CG_AA
Cuts at: 0 1577 2799
Size: 1577 1222
BstYI r'GATC_y
Cuts at: 0 232 2496 2576 2799
Size: 232 2264 80 223
Fragments arranged by size:
2264 232 223 80
Bsu36I CC'TnA_GG
Cuts at: 0 455 1103 2799
Size: 455 648 1696
Fragments arranged by size:
1696 648 455
BtgI C'CryG_G
Cuts at: 0 1615 1641 2075 2171 2639 2654 2799
Size: 1615 26 434 96 468 15 145
Fragments arranged by size:
1615 468 434 145 96 26 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1988 2140 2265 2799
Size: 1988 152 125 534
Fragments arranged by size:
1988 534 152 125
BtsI GCAGTG_nn'
Cuts at: 0 1804 2451 2799
Size: 1804 647 348
Fragments arranged by size:
1804 647 348
ClaI AT'CG_AT
Cuts at: 0 845 2799
Size: 845 1954
DraI TTT'AAA
Cuts at: 0 1206 2799
Size: 1206 1593
DraIII CAC_nnn'GTG
Cuts at: 0 837 2799
Size: 837 1962
DrdI GACnn_nn'nnGTC
Cuts at: 0 2296 2799
Size: 2296 503
EaeI y'GGCC_r
Cuts at: 0 131 642 672 1386 1763 2360 2485 2636
Size: 131 511 30 714 377 597 125 151
Cuts at: 2636 2651 2799
Size: 15 148
Fragments arranged by size:
714 597 511 377 151 148 131 125
30 15
EarI CTCTTCn'nnn_
Cuts at: 0 28 2799
Size: 28 2771
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1186 1965 2799
Size: 1186 779 834
Fragments arranged by size:
1186 834 779
EcoICRI GAG'CTC
Cuts at: 0 1063 2253 2685 2799
Size: 1063 1190 432 114
Fragments arranged by size:
1190 1063 432 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 43 109 130 386 517 634 1135
Size: 33 10 66 21 256 131 117 501
Cuts at: 1135 1161 1873 2017 2799
Size: 26 712 144 782
Fragments arranged by size:
782 712 501 256 144 131 117 66
33 26 21 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 855 2799
Size: 91 764 1944
Fragments arranged by size:
1944 764 91
EcoO109I rG'GnC_Cy
Cuts at: 0 102 186 336 451 860 933 995 1659
Size: 102 84 150 115 409 73 62 664
Cuts at: 1659 2123 2799
Size: 464 676
Fragments arranged by size:
676 664 464 409 150 115 102 84
73 62
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 744 2472 2799
Size: 744 1728 327
Fragments arranged by size:
1728 744 327
HaeII r_GCGC'y
Cuts at: 0 636 1258 1686 2799
Size: 636 622 428 1113
Fragments arranged by size:
1113 636 622 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 338 370 420 452 1590 1622
Size: 133 32 173 32 50 32 1138 32
Cuts at: 1622 1822 1854 2115 2147 2495 2527 2725 2757
Size: 200 32 261 32 348 32 198 32
Cuts at: 2757 2799
Size: 42
Fragments arranged by size:
1138 348 261 200 198 173 133 50
42 32 32 32 32 32 32 32
32
MluI A'CGCG_T
Cuts at: 0 2605 2799
Size: 2605 194
MscI TGG'CCA
Cuts at: 0 133 644 2653 2799
Size: 133 511 2009 146
Fragments arranged by size:
2009 511 146 133
MslI CAynn'nnrTG
Cuts at: 0 900 965 1133 1282 2799
Size: 900 65 168 149 1517
Fragments arranged by size:
1517 900 168 149 65
MspA1I CmG'CkG
Cuts at: 0 230 579 957 1391 1444 1563 1835 2585
Size: 230 349 378 434 53 119 272 750
Cuts at: 2585 2757 2799
Size: 172 42
Fragments arranged by size:
750 434 378 349 272 230 172 119
53 42
NcoI C'CATG_G
Cuts at: 0 1615 1641 2171 2799
Size: 1615 26 530 628
Fragments arranged by size:
1615 628 530 26
NspI r_CATG'y
Cuts at: 0 475 744 2799
Size: 475 269 2055
Fragments arranged by size:
2055 475 269
PciI A'CATG_T
Cuts at: 0 740 2799
Size: 740 2059
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 707 1553 2799
Size: 207 500 846 1246
Fragments arranged by size:
1246 846 500 207
PfoI T'CCnGG_A
Cuts at: 0 289 563 590 1788 2624 2799
Size: 289 274 27 1198 836 175
Fragments arranged by size:
1198 836 289 274 175 27
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 237 269 1909 1941 2381 2413 2799
Size: 237 32 1640 32 440 32 386
Fragments arranged by size:
1640 440 386 237 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 102 186 336 451 860 995 1659 2123
Size: 102 84 150 115 409 135 664 464
Cuts at: 2123 2799
Size: 676
Fragments arranged by size:
676 664 464 409 150 135 115 102
84
PspOMI G'GGCC_C
Cuts at: 0 905 933 1316 2799
Size: 905 28 383 1483
Fragments arranged by size:
1483 905 383 28
PvuII CAG'CTG
Cuts at: 0 579 957 1444 1563 2799
Size: 579 378 487 119 1236
Fragments arranged by size:
1236 579 487 378 119
SacI G_AGCT'C
Cuts at: 0 1065 2255 2687 2799
Size: 1065 1190 432 112
Fragments arranged by size:
1190 1065 432 112
SanDI GG'GwC_CC
Cuts at: 0 186 2799
Size: 186 2613
SexAI A'CCwGG_T
Cuts at: 0 2705 2799
Size: 2705 94
SfcI C'TryA_G
Cuts at: 0 952 1242 1530 2799
Size: 952 290 288 1269
Fragments arranged by size:
1269 952 290 288
SmaI CCC'GGG
Cuts at: 0 445 1657 2799
Size: 445 1212 1142
Fragments arranged by size:
1212 1142 445
SmlI C'TyrA_G
Cuts at: 0 748 871 1064 1159 2436 2722 2799
Size: 748 123 193 95 1277 286 77
Fragments arranged by size:
1277 748 286 193 123 95 77
StyI C'CwwG_G
Cuts at: 0 167 236 998 1223 1422 1547 1615 1641
Size: 167 69 762 225 199 125 68 26
Cuts at: 1641 1706 2171 2648 2669 2799
Size: 65 465 477 21 130
Fragments arranged by size:
762 477 465 225 199 167 130 125
69 68 65 26 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1310 1545 2666 2704 2799
Size: 1310 235 1121 38 95
Fragments arranged by size:
1310 1121 235 95 38
TatI w'GTAC_w
Cuts at: 0 737 1715 2799
Size: 737 978 1084
Fragments arranged by size:
1084 978 737
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 170 202 224 256 2646 2678 2799
Size: 170 32 22 32 2390 32 121
Fragments arranged by size:
2390 170 121 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 0 525 2799
Size: 525 2274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 652 708 2799
Size: 652 56 2091
Fragments arranged by size:
2091 652 56
XhoI C'TCGA_G
Cuts at: 0 748 2799
Size: 748 2051
XmaI C'CCGG_G
Cuts at: 0 443 1655 2799
Size: 443 1212 1144
Fragments arranged by size:
1212 1144 443
XmnI GAAnn'nnTTC
Cuts at: 0 1924 2799
Size: 1924 875
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BcgI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BsrGI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I MluI MscI MslI MspA1I
NcoI NspI PciI PflMI PfoI PpiI PpuMI PspOMI
PvuII SacI SanDI SexAI SfcI SmaI SmlI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BlpI BmgBI BmtI BsaHI
BsaXI BsiWI BsrBI BsrDI BssHII BssSI BstEII BstXI
BstZ17I CspCI EagI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI ZraI