(Circular) (Six-base) MAPSORT of: pFN21AB8407.seq Check: 8396 from: 1 to: 5587 pFN21AB8407 5587 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3397 278 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2930 1937 Size: 993 4594 Acc65I G'GTAC_C Cuts at: 2908 2908 Size: 5587 AclI AA'CG_TT Cuts at: 1563 3714 4087 1563 Size: 2151 373 3063 Fragments arranged by size: 3063 2151 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2516 3650 5034 5302 1632 Size: 884 1134 1384 268 1917 Fragments arranged by size: 1917 1384 1134 884 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5390 AflIII A'CryG_T Cuts at: 1203 2732 4486 5463 1203 Size: 1529 1754 977 1327 Fragments arranged by size: 1754 1529 1327 977 AgeI A'CCGG_T Cuts at: 4418 4418 Size: 5587 AhdI GACnn_n'nnGTC Cuts at: 4316 4316 Size: 5587 AleI CACnn'nnGTG Cuts at: 1166 2513 1166 Size: 1347 4240 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5587 AlwNI CAG_nnn'CTG Cuts at: 2269 2571 3330 4902 2269 Size: 302 759 1572 2954 Fragments arranged by size: 2954 1572 759 302 ApaLI G'TGCA_C Cuts at: 3644 4800 3644 Size: 1156 4431 ApoI r'AATT_y Cuts at: 1493 2896 3122 3274 5383 1493 Size: 1403 226 152 2109 1697 Fragments arranged by size: 2109 1697 1403 226 152 AscI GG'CGCG_CC Cuts at: 2126 2126 Size: 5587 AseI AT'TA_AT Cuts at: 160 1032 4141 160 Size: 872 3109 1606 Fragments arranged by size: 3109 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 AvaI C'yCGr_G Cuts at: 1870 1943 2912 1870 Size: 73 969 4545 Fragments arranged by size: 4545 969 73 BamHI G'GATC_C Cuts at: 2917 3284 2917 Size: 367 5220 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2908 4363 618 Size: 325 217 627 366 755 1455 1842 Fragments arranged by size: 1842 1455 755 627 366 325 217 BanII G_rGCy'C Cuts at: 1391 2906 1391 Size: 1515 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2477 2889 928 Size: 971 578 412 3626 Fragments arranged by size: 3626 971 578 412 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3778 3812 1903 Size: 34 1841 34 3678 Fragments arranged by size: 3678 1841 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2022 3479 4689 2022 Size: 1457 1210 2920 Fragments arranged by size: 2920 1457 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5587 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4198 136 Size: 107 122 71 631 605 2526 1525 Fragments arranged by size: 2526 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5582 5582 Size: 5587 BlpI GC'TnA_GC Cuts at: 3004 3004 Size: 5587 Bme1580I G_kGCm'C Cuts at: 1790 2158 3648 4804 1790 Size: 368 1490 1156 2573 Fragments arranged by size: 2573 1490 1156 368 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5587 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2218 2446 3318 Size: 416 53 83 399 24 801 228 872 Cuts at: 3318 4276 442 Size: 958 1753 Fragments arranged by size: 1753 958 872 801 416 399 228 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5587 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 4247 1239 Size: 863 2145 2579 Fragments arranged by size: 2579 2145 863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3712 4577 4875 3712 Size: 865 298 4424 Fragments arranged by size: 4424 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2636 4250 882 Size: 617 1137 1614 2219 Fragments arranged by size: 2219 1614 1137 617 BsaAI yAC'GTr Cuts at: 493 1204 3378 493 Size: 711 2174 2702 Fragments arranged by size: 2702 2174 711 BsaBI GATnn'nnATC Cuts at: 5581 5581 Size: 5587 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3394 3776 4411 275 Size: 53 83 186 936 1861 382 635 1451 Fragments arranged by size: 1861 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2150 4020 4418 4692 4839 5250 2150 Size: 1870 398 274 147 411 2487 Fragments arranged by size: 2487 1870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5362 Fragments arranged by size: 5362 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5587 BseYI C'CCAG_C Cuts at: 2157 4790 5286 2157 Size: 2633 496 2458 Fragments arranged by size: 2633 2458 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 1163 Size: 573 315 4699 Fragments arranged by size: 4699 573 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 3037 3798 4826 1540 Size: 275 186 54 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 275 186 54 BsiHKAI G_wGCw'C Cuts at: 2187 3648 3733 4804 2187 Size: 1461 85 1071 2970 Fragments arranged by size: 2970 1461 1071 85 BsmI GAATG_Cn' Cuts at: 3098 3191 3098 Size: 93 5494 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2187 2906 3648 3733 4804 1391 Size: 399 368 29 719 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 719 399 368 85 29 BspEI T'CCGG_A Cuts at: 5250 5250 Size: 5587 BspHI T'CATG_A Cuts at: 3476 5206 3476 Size: 1730 3857 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2942 844 Size: 2098 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2051 3474 1394 Size: 429 228 1423 3507 Fragments arranged by size: 3507 1423 429 228 BsrDI GCAATG_nn' Cuts at: 65 1248 4082 4256 65 Size: 1183 2834 174 1396 Fragments arranged by size: 2834 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2161 4231 4418 1700 Size: 110 210 141 2070 187 2869 Fragments arranged by size: 2869 2070 210 187 141 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5587 BssHII G'CGCG_C Cuts at: 1925 2126 1925 Size: 201 5386 BssSI C'ACGA_G Cuts at: 3647 4659 3647 Size: 1012 4575 BstAPI GCAn_nnn'nTGC Cuts at: 794 2571 794 Size: 1777 3810 BstBI TT'CG_AA Cuts at: 4391 4391 Size: 5587 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5587 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 5587 BstYI r'GATC_y Cuts at: 1972 2917 3284 3669 3686 5138 5224 5236 5582 Size: 945 367 385 17 1452 86 12 346 Cuts at: 5582 1972 Size: 1977 Fragments arranged by size: 1977 1452 945 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 2037 2326 513 Size: 552 939 21 12 289 3774 Fragments arranged by size: 3774 939 552 289 21 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4848 Fragments arranged by size: 4848 683 56 BtsI GCAGTG_nn' Cuts at: 3103 3897 3917 3103 Size: 794 20 4773 Fragments arranged by size: 4773 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5587 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2796 2831 529 Size: 35 2232 35 3285 Fragments arranged by size: 3285 2232 35 35 DraI TTT'AAA Cuts at: 2891 2891 Size: 5587 DraIII CAC_nnn'GTG Cuts at: 2226 2226 Size: 5587 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2690 4594 809 Size: 507 1374 1904 1802 Fragments arranged by size: 1904 1802 1374 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2052 3034 Size: 54 1425 50 275 18 192 30 982 Cuts at: 3034 3923 5552 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 982 889 275 192 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2052 3034 1537 Size: 275 240 982 4090 Fragments arranged by size: 4090 982 275 240 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2303 2372 3517 1353 Size: 863 87 69 1145 3423 Fragments arranged by size: 3423 1145 863 87 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2061 2696 4174 4548 4694 387 Size: 1064 138 472 635 1478 374 146 1280 Fragments arranged by size: 1478 1280 1064 635 472 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2516 3650 4247 5034 5302 1239 Size: 393 470 414 1134 597 787 268 1524 Fragments arranged by size: 1524 1134 787 597 470 414 393 268 EcoRI G'AATT_C Cuts at: 2896 3274 2896 Size: 378 5209 FspI TGC'GCA Cuts at: 4093 4093 Size: 5587 HaeII r_GCGC'y Cuts at: 1678 4734 1678 Size: 3056 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4228 4260 4302 4334 1373 Size: 32 135 32 2656 32 42 32 2626 Fragments arranged by size: 2656 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2931 3177 669 Size: 988 281 993 246 3079 Fragments arranged by size: 3079 993 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5587 HpaI GTT'AAC Cuts at: 3177 3177 Size: 5587 KpnI G_GTAC'C Cuts at: 2912 2912 Size: 5587 MfeI C'AATT_G Cuts at: 3186 3186 Size: 5587 MluI A'CGCG_T Cuts at: 5463 5463 Size: 5587 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4701 4885 1305 Size: 3396 184 2007 Fragments arranged by size: 3396 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3765 Fragments arranged by size: 3765 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 3545 3904 4063 518 Size: 648 1347 1032 359 159 2042 Fragments arranged by size: 2042 1347 1032 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2708 2781 3003 3680 4828 5073 1606 Size: 433 669 73 222 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 669 433 245 222 73 NaeI GCC'GGC Cuts at: 2022 2022 Size: 5587 NcoI C'CATG_G Cuts at: 513 1065 2004 2326 513 Size: 552 939 322 3774 Fragments arranged by size: 3774 939 552 322 NdeI CA'TA_TG Cuts at: 387 387 Size: 5587 NgoMIV G'CCGG_C Cuts at: 2020 2020 Size: 5587 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5587 NotI GC'GGCC_GC Cuts at: 3034 3034 Size: 5587 NspI r_CATG'y Cuts at: 1132 1229 2297 2736 2945 4490 1132 Size: 97 1068 439 209 1545 2229 Fragments arranged by size: 2229 1545 1068 439 209 97 PasI CC'CwG_GG Cuts at: 1348 2827 1348 Size: 1479 4108 PciI A'CATG_T Cuts at: 2732 4486 2732 Size: 1754 3833 PflMI CCAn_nnn'nTGG Cuts at: 1108 2164 2269 2332 1108 Size: 1056 105 63 4363 Fragments arranged by size: 4363 1056 105 63 PmeI GTTT'AAAC Cuts at: 2891 2891 Size: 5587 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3656 3688 5169 5201 3656 Size: 32 1481 32 4042 Fragments arranged by size: 4042 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5587 PsiI TTA'TAA Cuts at: 3157 3157 Size: 5587 PstI C_TGCA'G Cuts at: 830 2567 2939 830 Size: 1737 372 3478 Fragments arranged by size: 3478 1737 372 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5587 PvuII CAG'CTG Cuts at: 2708 2708 Size: 5587 SacII CC_GC'GG Cuts at: 2040 2040 Size: 5587 SalI G'TCGA_C Cuts at: 1936 2929 1936 Size: 993 4594 SapI GCTCTTCn'nnn_ Cuts at: 2372 2372 Size: 5587 SbfI CC_TGCA'GG Cuts at: 2939 2939 Size: 5587 ScaI AGT'ACT Cuts at: 3835 5547 3835 Size: 1712 3875 SfcI C'TryA_G Cuts at: 826 1045 2563 2935 4070 4751 4942 826 Size: 219 1518 372 1135 681 191 1471 Fragments arranged by size: 1518 1471 1135 681 372 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5587 SmaI CCC'GGG Cuts at: 1872 2914 1872 Size: 1042 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3691 4592 4854 820 Size: 197 926 1748 901 262 1553 Fragments arranged by size: 1748 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3378 493 Size: 2885 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5587 SphI G_CATG'C Cuts at: 1132 2945 1132 Size: 1813 3774 SspI AAT'ATT Cuts at: 5 52 3511 5 Size: 47 3459 2081 Fragments arranged by size: 3459 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5587 StyI C'CwwG_G Cuts at: 513 1065 2004 2326 2877 3026 513 Size: 552 939 322 551 149 3074 Fragments arranged by size: 3074 939 552 551 322 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2237 3614 3631 3784 3969 4414 325 Size: 1255 657 1377 17 153 185 445 1498 Fragments arranged by size: 1498 1377 1255 657 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3833 5545 96 Size: 274 80 33 51 1443 1856 1712 138 Fragments arranged by size: 1856 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2686 2718 5547 5579 2686 Size: 32 2829 32 2694 Fragments arranged by size: 2829 2694 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2594 1316 Size: 1278 4309 XbaI T'CTAG_A Cuts at: 2923 2923 Size: 5587 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5587 XmaI C'CCGG_G Cuts at: 1870 2912 1870 Size: 1042 4545 XmnI GAAnn'nnTTC Cuts at: 3716 3716 Size: 5587 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3395 276 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI