(Circular) (Six-base) MAPSORT of: pFN21AB8404.seq Check: 5066 from: 1 to: 8245 pFN21AB8404 8245 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2503 2819 2503 Size: 316 7929 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6055 278 Size: 53 83 186 936 4519 2468 Fragments arranged by size: 4519 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2292 3957 5588 1937 Size: 355 1665 1631 4594 Fragments arranged by size: 4594 1665 1631 355 Acc65I G'GTAC_C Cuts at: 2657 5566 2657 Size: 2909 5336 AclI AA'CG_TT Cuts at: 1563 4539 6372 6745 1563 Size: 2976 1833 373 3063 Fragments arranged by size: 3063 2976 1833 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5492 6308 7692 7960 1632 Size: 3860 816 1384 268 1917 Fragments arranged by size: 3860 1917 1384 816 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8048 AflIII A'CryG_T Cuts at: 1203 2851 3413 3942 4435 4568 7144 8121 1203 Size: 1648 562 529 493 133 2576 977 1327 Fragments arranged by size: 2576 1648 1327 977 562 529 493 133 AgeI A'CCGG_T Cuts at: 7076 7076 Size: 8245 AhdI GACnn_n'nnGTC Cuts at: 3558 6974 3558 Size: 3416 4829 AleI CACnn'nnGTG Cuts at: 1166 2316 5015 5240 1166 Size: 1150 2699 225 4171 Fragments arranged by size: 4171 2699 1150 225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2226 2334 4305 4936 5143 1184 Size: 1042 108 1971 631 207 4286 Fragments arranged by size: 4286 1971 1042 631 207 108 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2567 2599 2567 Size: 32 8213 AlwNI CAG_nnn'CTG Cuts at: 2371 5090 5136 5988 7560 2371 Size: 2719 46 852 1572 3056 Fragments arranged by size: 3056 2719 1572 852 46 ApaI G_GGCC'C Cuts at: 2954 3003 3159 4625 5276 2954 Size: 49 156 1466 651 5923 Fragments arranged by size: 5923 1466 651 156 49 ApaLI G'TGCA_C Cuts at: 6302 7458 6302 Size: 1156 7089 ApoI r'AATT_y Cuts at: 1493 5554 5780 5932 8041 1493 Size: 4061 226 152 2109 1697 Fragments arranged by size: 4061 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6799 160 Size: 872 5767 1606 Fragments arranged by size: 5767 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8245 AvaI C'yCGr_G Cuts at: 1870 1943 3340 3978 4236 5276 5570 1870 Size: 73 1397 638 258 1040 294 4545 Fragments arranged by size: 4545 1397 1040 638 294 258 73 BamHI G'GATC_C Cuts at: 4839 5575 5942 4839 Size: 736 367 7142 Fragments arranged by size: 7142 736 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2657 3242 3747 3830 4178 Size: 325 217 627 870 585 505 83 348 Cuts at: 4178 4501 4513 4600 4723 4851 5566 7021 618 Size: 323 12 87 123 128 715 1455 1842 Fragments arranged by size: 1842 1455 870 715 627 585 505 348 325 323 217 128 123 87 83 12 BanII G_rGCy'C Cuts at: 1391 2670 2884 2941 2954 3003 3159 3458 3898 Size: 1279 214 57 13 49 156 299 440 Cuts at: 3898 4625 5153 5168 5195 5276 5564 1391 Size: 727 528 15 27 81 288 4072 Fragments arranged by size: 4072 1279 727 528 440 299 288 214 156 81 57 49 27 15 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2849 4454 928 Size: 971 950 1605 4719 Fragments arranged by size: 4719 1605 971 950 BbvCI CC'TCA_GC Cuts at: 3732 3918 4376 3732 Size: 186 458 7601 Fragments arranged by size: 7601 458 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2797 2831 5318 5352 5490 5524 6436 Size: 34 860 34 2487 34 138 34 912 Cuts at: 6436 6470 1903 Size: 34 3678 Fragments arranged by size: 3678 2487 912 860 138 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3159 6137 7347 3159 Size: 2978 1210 4057 Fragments arranged by size: 4057 2978 1210 BclI T'GATC_A Cuts at: 1548 3006 4794 1548 Size: 1458 1788 4999 Fragments arranged by size: 4999 1788 1458 BfrBI ATG'CAT Cuts at: 4153 5530 4153 Size: 1377 6868 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4371 4732 6856 Size: 107 122 71 631 605 2699 361 2124 Cuts at: 6856 136 Size: 1525 Fragments arranged by size: 2699 2124 1525 631 605 361 122 107 71 BglII A'GATC_T Cuts at: 8240 8240 Size: 8245 BlpI GC'TnA_GC Cuts at: 3795 5662 3795 Size: 1867 6378 Bme1580I G_kGCm'C Cuts at: 1790 2863 2954 3003 3159 4576 4605 4625 5267 Size: 1073 91 49 156 1417 29 20 642 Cuts at: 5267 5276 6306 7462 1790 Size: 9 1030 1156 2573 Fragments arranged by size: 2573 1417 1156 1073 1030 642 156 91 49 29 20 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8245 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2086 2121 2575 Size: 416 53 83 399 24 669 35 454 Cuts at: 2575 2614 2747 3101 3452 3517 3783 5976 6934 Size: 39 133 354 351 65 266 2193 958 Cuts at: 6934 442 Size: 1753 Fragments arranged by size: 2193 1753 958 669 454 416 399 354 351 266 133 83 65 53 39 35 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8245 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2851 2851 Size: 8245 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2365 2431 3008 3217 5137 5179 5395 Size: 855 271 66 577 209 1920 42 216 Cuts at: 5395 6905 1239 Size: 1510 2579 Fragments arranged by size: 2579 1920 1510 855 577 271 216 209 66 42 Bpu10I CC'TnA_GC Cuts at: 2652 3732 3918 4376 5147 2652 Size: 1080 186 458 771 5750 Fragments arranged by size: 5750 1080 771 458 186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2165 3533 6370 7235 7533 2165 Size: 1368 2837 865 298 2877 Fragments arranged by size: 2877 2837 1368 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2570 2920 2960 3046 4039 6908 882 Size: 617 1071 350 40 86 993 2869 2219 Fragments arranged by size: 2869 2219 1071 993 617 350 86 40 BsaAI yAC'GTr Cuts at: 493 1204 3943 4266 4438 4571 5013 6036 493 Size: 711 2739 323 172 133 442 1023 2702 Fragments arranged by size: 2739 2702 1023 711 442 323 172 133 BsaBI GATnn'nnATC Cuts at: 4781 4799 8239 4781 Size: 18 3440 4787 Fragments arranged by size: 4787 3440 18 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2228 4812 6052 6434 Size: 53 83 186 936 695 2584 1240 382 Cuts at: 6434 7069 275 Size: 635 1451 Fragments arranged by size: 2584 1451 1240 936 695 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2033 2530 2884 6678 7076 7350 7497 7908 2033 Size: 497 354 3794 398 274 147 411 2370 Fragments arranged by size: 3794 2370 497 411 398 354 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2567 2597 4298 4328 1178 Size: 30 165 30 1164 30 1701 30 5095 Fragments arranged by size: 5095 1701 1164 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 3466 4787 1186 Size: 1309 971 1321 4644 Fragments arranged by size: 4644 1321 1309 971 BseYI C'CCAG_C Cuts at: 2166 2644 3136 3391 3517 3890 4276 4416 4575 Size: 478 492 255 126 373 386 140 159 Cuts at: 4575 5023 5171 7448 7944 2166 Size: 448 148 2277 496 2467 Fragments arranged by size: 2467 2277 496 492 478 448 386 373 255 159 148 140 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2340 2696 3159 5267 1163 Size: 573 604 356 463 2108 4141 Fragments arranged by size: 4141 2108 604 573 463 356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2059 5695 6456 7484 1540 Size: 275 186 58 3636 761 1028 2301 Fragments arranged by size: 3636 2301 1028 761 275 186 58 BsiHKAI G_wGCw'C Cuts at: 4326 5153 6306 6391 7462 4326 Size: 827 1153 85 1071 5109 Fragments arranged by size: 5109 1153 1071 827 85 BsmI GAATG_Cn' Cuts at: 4153 5756 5849 4153 Size: 1603 93 6549 Fragments arranged by size: 6549 1603 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2670 2863 2884 2941 2954 3003 3159 Size: 399 880 193 21 57 13 49 156 Cuts at: 3159 3458 3898 4326 4576 4605 4625 5153 5168 Size: 299 440 428 250 29 20 528 15 Cuts at: 5168 5195 5267 5276 5564 6306 6391 7462 1391 Size: 27 72 9 288 742 85 1071 2174 Fragments arranged by size: 2174 1071 880 742 528 440 428 399 299 288 250 193 156 85 72 57 49 29 27 21 20 15 13 9 BspEI T'CCGG_A Cuts at: 2530 2884 7908 2530 Size: 354 5024 2867 Fragments arranged by size: 5024 2867 354 BspHI T'CATG_A Cuts at: 3315 6134 7864 3315 Size: 2819 1730 3696 Fragments arranged by size: 3696 2819 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2503 2819 5600 844 Size: 1659 316 2781 3489 Fragments arranged by size: 3489 2781 1659 316 BsrBI CCG'CTC Cuts at: 1394 1823 2120 6132 1394 Size: 429 297 4012 3507 Fragments arranged by size: 4012 3507 429 297 BsrDI GCAATG_nn' Cuts at: 65 1248 2053 3404 6740 6914 65 Size: 1183 805 1351 3336 174 1396 Fragments arranged by size: 3336 1396 1351 1183 805 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 4520 6889 7076 1700 Size: 110 244 2466 2369 187 2869 Fragments arranged by size: 2869 2466 2369 244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8245 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8245 BssSI C'ACGA_G Cuts at: 2075 2171 6305 7317 2075 Size: 96 4134 1012 3003 Fragments arranged by size: 4134 3003 1012 96 BstAPI GCAn_nnn'nTGC Cuts at: 794 5136 794 Size: 4342 3903 BstBI TT'CG_AA Cuts at: 7049 7049 Size: 8245 BstEII G'GTnAC_C Cuts at: 1184 2731 3382 3466 1184 Size: 1547 651 84 5963 Fragments arranged by size: 5963 1547 651 84 BstXI CCAn_nnnn'nTGG Cuts at: 2134 5242 2134 Size: 3108 5137 BstYI r'GATC_y Cuts at: 1972 3210 4776 4839 5388 5575 5942 6327 6344 Size: 1238 1566 63 549 187 367 385 17 Cuts at: 6344 7796 7882 7894 8240 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1566 1452 1238 549 385 367 346 187 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 3291 3634 3291 Size: 343 7902 BtgI C'CryG_G Cuts at: 513 1065 3248 4429 4626 4816 4863 513 Size: 552 2183 1181 197 190 47 3895 Fragments arranged by size: 3895 2183 1181 552 197 190 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3365 5081 5321 487 Size: 683 56 2139 1716 240 3411 Fragments arranged by size: 3411 2139 1716 683 240 56 BtsI GCAGTG_nn' Cuts at: 3885 4797 5289 5761 6555 6575 3885 Size: 912 492 472 794 20 5555 Fragments arranged by size: 5555 912 794 492 472 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8245 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8210 DraI TTT'AAA Cuts at: 5549 5549 Size: 8245 DraIII CAC_nnn'GTG Cuts at: 2094 2094 Size: 8245 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2603 2896 3965 4942 7252 809 Size: 507 1287 293 1069 977 2310 1802 Fragments arranged by size: 2310 1802 1287 1069 977 507 293 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 2391 3537 Size: 54 1425 50 275 18 226 335 1146 Cuts at: 3537 4053 4170 4522 4591 4648 5352 5511 5692 Size: 516 117 352 69 57 704 159 181 Cuts at: 5692 6581 8210 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1146 889 704 516 352 335 275 226 181 159 117 69 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2056 5692 1537 Size: 275 244 3636 4090 Fragments arranged by size: 4090 3636 275 244 EarI CTCTTCn'nnn_ Cuts at: 1353 2529 3314 6175 1353 Size: 1176 785 2861 3423 Fragments arranged by size: 3423 2861 1176 785 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2492 4918 5287 5359 5381 6832 Size: 1064 138 903 2426 369 72 22 1451 Cuts at: 6832 7206 7352 387 Size: 374 146 1280 Fragments arranged by size: 2426 1451 1280 1064 903 374 369 146 138 72 22 EcoICRI GAG'CTC Cuts at: 5151 5151 Size: 8245 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2365 2431 3008 3217 5137 5179 Size: 393 462 271 66 577 209 1920 42 Cuts at: 5179 5395 5492 6308 6905 7692 7960 1239 Size: 216 97 816 597 787 268 1524 Fragments arranged by size: 1920 1524 816 787 597 577 462 393 271 268 216 209 97 66 42 EcoNI CCTnn'n_nnAGG Cuts at: 2214 3329 2214 Size: 1115 7130 EcoO109I rG'GnC_Cy Cuts at: 2176 2681 2950 2951 2999 3155 3344 3778 4622 Size: 505 269 1 48 156 189 434 844 Cuts at: 4622 5272 5273 2176 Size: 650 1 5148 Fragments arranged by size: 5148 844 650 505 434 269 189 156 48 1 1 EcoRI G'AATT_C Cuts at: 5554 5932 5554 Size: 378 7867 FspI TGC'GCA Cuts at: 6751 6751 Size: 8245 HaeII r_GCGC'y Cuts at: 1678 2156 2422 4205 4366 4383 5489 7392 1678 Size: 478 266 1783 161 17 1106 1903 2531 Fragments arranged by size: 2531 1903 1783 1106 478 266 161 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3544 3576 6886 6918 6960 Size: 32 135 32 1972 32 3310 32 42 Cuts at: 6960 6992 1373 Size: 32 2626 Fragments arranged by size: 3310 2626 1972 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5589 5835 669 Size: 988 281 3651 246 3079 Fragments arranged by size: 3651 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8245 HpaI GTT'AAC Cuts at: 5835 5835 Size: 8245 KpnI G_GTAC'C Cuts at: 2661 5570 2661 Size: 2909 5336 MfeI C'AATT_G Cuts at: 5844 5844 Size: 8245 MluI A'CGCG_T Cuts at: 8121 8121 Size: 8245 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3596 4371 7359 7543 1305 Size: 2291 775 2988 184 2007 Fragments arranged by size: 2988 2291 2007 775 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3539 4172 4650 10 Size: 54 1425 343 1707 633 478 3605 Fragments arranged by size: 3605 1707 1425 633 478 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2316 2564 3945 5015 5217 5240 6203 Size: 648 1150 248 1381 1070 202 23 963 Cuts at: 6203 6562 6721 518 Size: 359 159 2042 Fragments arranged by size: 2042 1381 1150 1070 963 648 359 248 202 159 23 MspA1I CmG'CkG Cuts at: 1606 3794 3988 4063 4628 5308 5661 6338 7486 Size: 2188 194 75 565 680 353 677 1148 Cuts at: 7486 7731 1606 Size: 245 2120 Fragments arranged by size: 2188 2120 1148 680 677 565 353 245 194 75 NaeI GCC'GGC Cuts at: 2056 2056 Size: 8245 NcoI C'CATG_G Cuts at: 513 1065 4863 513 Size: 552 3798 3895 Fragments arranged by size: 3895 3798 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8245 NgoMIV G'CCGG_C Cuts at: 2054 2054 Size: 8245 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8245 NotI GC'GGCC_GC Cuts at: 5692 5692 Size: 8245 NsiI A_TGCA'T Cuts at: 4155 5532 4155 Size: 1377 6868 NspI r_CATG'y Cuts at: 1132 1229 3189 3261 3417 4536 5530 5603 7148 Size: 97 1960 72 156 1119 994 73 1545 Cuts at: 7148 1132 Size: 2229 Fragments arranged by size: 2229 1960 1545 1119 994 156 97 73 72 PasI CC'CwG_GG Cuts at: 1348 2947 3783 4389 1348 Size: 1599 836 606 5204 Fragments arranged by size: 5204 1599 836 606 PciI A'CATG_T Cuts at: 3413 7144 3413 Size: 3731 4514 PflMI CCAn_nnn'nTGG Cuts at: 1108 2386 1108 Size: 1278 6967 PfoI T'CCnGG_A Cuts at: 2133 2403 3766 3902 5064 5153 2133 Size: 270 1363 136 1162 89 5225 Fragments arranged by size: 5225 1363 1162 270 136 89 PmeI GTTT'AAAC Cuts at: 5549 5549 Size: 8245 PmlI CAC'GTG Cuts at: 3943 4266 4438 4571 5013 3943 Size: 323 172 133 442 7175 Fragments arranged by size: 7175 442 323 172 133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2567 2599 6314 6346 7827 7859 2567 Size: 32 3715 32 1481 32 2953 Fragments arranged by size: 3715 2953 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2681 3778 2681 Size: 1097 7148 PshAI GACnn'nnGTC Cuts at: 1613 2620 4137 1613 Size: 1007 1517 5721 Fragments arranged by size: 5721 1517 1007 PsiI TTA'TAA Cuts at: 5815 5815 Size: 8245 PspOMI G'GGCC_C Cuts at: 2950 2999 3155 4621 5272 2950 Size: 49 156 1466 651 5923 Fragments arranged by size: 5923 1466 651 156 49 PstI C_TGCA'G Cuts at: 830 2615 2640 3793 4771 4925 5597 830 Size: 1785 25 1153 978 154 672 3478 Fragments arranged by size: 3478 1785 1153 978 672 154 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8245 PvuII CAG'CTG Cuts at: 3794 3988 5308 3794 Size: 194 1320 6731 Fragments arranged by size: 6731 1320 194 RsrII CG'GwC_CG Cuts at: 4737 4737 Size: 8245 SacI G_AGCT'C Cuts at: 5153 5153 Size: 8245 SacII CC_GC'GG Cuts at: 4629 4629 Size: 8245 SalI G'TCGA_C Cuts at: 1936 5587 1936 Size: 3651 4594 SanDI GG'GwC_CC Cuts at: 3778 3778 Size: 8245 SapI GCTCTTCn'nnn_ Cuts at: 3314 3314 Size: 8245 SbfI CC_TGCA'GG Cuts at: 5597 5597 Size: 8245 ScaI AGT'ACT Cuts at: 6493 8205 6493 Size: 1712 6533 SfcI C'TryA_G Cuts at: 826 1045 2067 2611 2636 3789 3954 4767 4921 Size: 219 1022 544 25 1153 165 813 154 Cuts at: 4921 4960 5593 6728 7409 7600 826 Size: 39 633 1135 681 191 1471 Fragments arranged by size: 1471 1153 1135 1022 813 681 633 544 219 191 165 154 39 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8245 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8245 SmaI CCC'GGG Cuts at: 1872 3342 3980 4238 5278 5572 1872 Size: 1470 638 258 1040 294 4545 Fragments arranged by size: 4545 1470 1040 638 294 258 SmlI C'TyrA_G Cuts at: 820 1017 1943 2180 3548 6349 7250 7512 820 Size: 197 926 237 1368 2801 901 262 1553 Fragments arranged by size: 2801 1553 1368 926 901 262 237 197 SnaBI TAC'GTA Cuts at: 493 6036 493 Size: 5543 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8245 SphI G_CATG'C Cuts at: 1132 5603 1132 Size: 4471 3774 SspI AAT'ATT Cuts at: 5 52 6169 5 Size: 47 6117 2081 Fragments arranged by size: 6117 2081 47 StuI AGG'CCT Cuts at: 1517 2273 2411 4073 1517 Size: 756 138 1662 5689 Fragments arranged by size: 5689 1662 756 138 StyI C'CwwG_G Cuts at: 513 1065 2187 2394 3660 3666 4068 4338 4863 Size: 552 1122 207 1266 6 402 270 525 Cuts at: 4863 5684 513 Size: 821 3074 Fragments arranged by size: 3074 1266 1122 821 552 525 402 270 207 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 3109 3673 3848 4325 4531 5005 Size: 1255 525 1004 564 175 477 206 474 Cuts at: 5005 5067 6272 6289 6442 6627 7072 325 Size: 62 1205 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1205 1004 564 525 477 474 445 206 185 175 153 62 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6491 8203 96 Size: 274 80 33 51 1443 4514 1712 138 Fragments arranged by size: 4514 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8205 8237 8205 Size: 32 8213 Tth111I GACn'n_nGTC Cuts at: 1316 2227 4231 1316 Size: 911 2004 5330 Fragments arranged by size: 5330 2004 911 XbaI T'CTAG_A Cuts at: 5581 5581 Size: 8245 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2455 3877 4367 2455 Size: 1422 490 6333 Fragments arranged by size: 6333 1422 490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8245 XmaI C'CCGG_G Cuts at: 1870 3340 3978 4236 5276 5570 1870 Size: 1470 638 258 1040 294 4545 Fragments arranged by size: 4545 1470 1040 638 294 258 XmnI GAAnn'nnTTC Cuts at: 6374 6374 Size: 8245 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6053 276 Size: 53 83 186 936 4519 2468 Fragments arranged by size: 4519 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI PacI PspXI PsrI SexAI SfiI SfoI SrfI SwaI