Restriction map for
pFN21AB8402
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp718I AspEI AspI AvrII BalI
BbeI BbrPI BbsI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoO65I EcoRV
FbaI HincII HindII HpaI KasI
KpnI Ksp632I MamI MfeI MluI
MluNI MscI MslI MunI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI ScaI SexAI SfoI SfuI
SmaI SmlI SnaBI SpeI SspBI
SspI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 532 GACGTC GACGT^C
AflIII 640 ACACGT A^C[GA][CT]GT
AviII 1197 TGCGCA TGC^GCA
BamHI 373 GGATCC G^GATCC
BbvCI 2157 GCTGAGG CCTCAGC(-5/-2)
BcnI 957 CCGGG CC^[GC]GG
BsaAI 641 CACGTA [CT]AC^GT[GA]
BspMI 1236 GCAGGT ACCTGC(4/8)
BstAPI 893 GCACCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 503 CCAGAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI 315 GAATTC G^AATTC
EcoT22I 259 ATGCAT ATGCA^T
FseI 965 GGCCGGCC GGCCGG^CC
FspI 1197 TGCGCA TGC^GCA
HindIII 2236 AAGCTT A^AGCTT
KspI 987 CCGCGG CCGC^GG
MboII 516 TCTTC GAAGA(8/7)
MseI 2297 TTAA T^TAA
NaeI 966 GCCGGC GCC^GGC
NciI 957 CCGGG CC^[GC]GG
NgoMIV 966 GCCGGC G^CCGGC
NsiI 259 ATGCAT ATGCA^T
PflMI 1450 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 498 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 129 GACTCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 987 CCGCGG CCGC^GG
SfiI 2180 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 940 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1481 GCATGC GCATG^C
Tru9I 2297 TTAA T^TAA
Van91I 1450 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 360 CCAGTGTTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 803 TCCGGA T^CCGGA
1869 TCCGGA
AcsI 315 GAATTC [GA]^AATT[CT]
658 AAATTC
AcyI 433 GACGCC G[GA]^CG[CT]C
532 GACGTC
AfaI 706 GTAC GT^AC
2257 GTAC
ApaI 795 GGGCCC GGGCC^C
1099 GGGCCC
ApoI 315 GAATTC [GA]^AATT[CT]
658 AAATTC
Asp700I 1350 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2214 GAAGGGCTTC
BcgI 404 GCATCACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1292 GCAAGGGCTTCG
BsaHI 433 GACGCC G[GA]^CG[CT]C
532 GACGTC
BsaI 1266 GAGACC GGTCTC(1/5)
1770 GAGACC
BseAI 803 TCCGGA T^CCGGA
1869 TCCGGA
BseRI 246 CTCCTC GAGGAG(10/8)
443 CTCCTC
BsmBI 135 CGTCTC CGTCTC(1/5)
676 GAGACG
BspEI 803 TCCGGA T^CCGGA
1869 TCCGGA
BsrDI 1201 CATTGC GCAATG(2/0)
2323 GCAATG
BssSI 835 CACGAG CACGAG(-5/-1)
1695 CTCGTG
DsaI 688 CCGTGG C^C[GA][CT]GG
987 CCGCGG
EcoT14I 399 CCAAGG C^C[AT][AT]GG
562 CCTTGG
HgaI 433 GACGC GACGC(5/10)
678 GACGC
Hin1I 433 GACGCC G[GA]^CG[CT]C
532 GACGTC
MaeII 533 ACGT A^CGT
642 ACGT
MaeIII 2042 GTGAC ^GT[ACGT]AC
2126 GTGAC
MroI 803 TCCGGA T^CCGGA
1869 TCCGGA
PleI 129 GACTC GAGTC(4/5)
1334 GAGTC
RsaI 706 GTAC GT^AC
2257 GTAC
SbfI 1160 CCTGCAGG CCTGCA^GG
1277 CCTGCAGG
Sse8387I 1160 CCTGCAGG CCTGCA^GG
1277 CCTGCAGG
StuI 391 AGGCCT AGG^CCT
2104 AGGCCT
StyI 399 CCAAGG C^C[AT][AT]GG
562 CCTTGG
TaqI 1332 TCGA T^CGA
1991 TCGA
Tsp45I 2042 GTGAC ^GT[GC]AC
2126 GTGAC
TthHB8I 1332 TCGA T^CGA
1991 TCGA
XbaI 75 TCTAGA T^CTAGA
141 TCTAGA
XmnI 1350 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2214 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AluI Alw44I AlwI
AlwNI Aor51HI ApaLI AspHI AvaI
AvaII BanI BanII BbvI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BsaWI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
Eco47III EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MboI MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI TseI Tsp509I TspRI
XhoII