Restriction map for
pFN21AB7636
(Linear) (Six-base) MAPSORT of: KIBB7636.seq Check: 5380 from: 1 to: 1131
KIBB7636.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 0 752 1131
Size: 752 379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 1131
Size: 335 796
AflII C'TTAA_G
Cuts at: 0 862 1131
Size: 862 269
AflIII A'CryG_T
Cuts at: 0 638 1131
Size: 638 493
AlwNI CAG_nnn'CTG
Cuts at: 0 39 313 1131
Size: 39 274 818
Fragments arranged by size:
818 274 39
ApoI r'AATT_y
Cuts at: 0 502 604 1131
Size: 502 102 527
Fragments arranged by size:
527 502 102
AvaI C'yCGr_G
Cuts at: 0 64 1131
Size: 64 1067
AvrII C'CTAG_G
Cuts at: 0 654 966 1131
Size: 654 312 165
Fragments arranged by size:
654 312 165
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 660 693 1131
Size: 660 33 438
Fragments arranged by size:
660 438 33
BamHI G'GATC_C
Cuts at: 0 813 1131
Size: 813 318
BanII G_rGCy'C
Cuts at: 0 1076 1131
Size: 1076 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 563 1131
Size: 563 568
BclI T'GATC_A
Cuts at: 0 264 1131
Size: 264 867
BglI GCCn_nnn'nGGC
Cuts at: 0 254 931 1131
Size: 254 677 200
Fragments arranged by size:
677 254 200
BglII A'GATC_T
Cuts at: 0 725 1131
Size: 725 406
Bme1580I G_kGCm'C
Cuts at: 0 689 1131
Size: 689 442
BmgBI CAC'GTC
Cuts at: 0 639 1131
Size: 639 492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 434 824 1131
Size: 220 214 390 307
Fragments arranged by size:
390 307 220 214
BsaAI yAC'GTr
Cuts at: 0 522 1131
Size: 522 609
BsaBI GATnn'nnATC
Cuts at: 0 221 263 266 530 1131
Size: 221 42 3 264 601
Fragments arranged by size:
601 264 221 42 3
BsaWI w'CCGG_w
Cuts at: 0 270 1131
Size: 270 861
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 536 566 592 622 1131
Size: 536 30 26 30 509
Fragments arranged by size:
536 509 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 208 920 923 1131
Size: 19 189 712 3 208
Fragments arranged by size:
712 208 189 19 3
BseYI C'CCAG_C
Cuts at: 0 306 789 808 1131
Size: 306 483 19 323
Fragments arranged by size:
483 323 306 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 583 1131
Size: 520 63 548
Fragments arranged by size:
548 520 63
BsiHKAI G_wGCw'C
Cuts at: 0 826 1131
Size: 826 305
BsmI GAATG_Cn'
Cuts at: 0 123 562 1131
Size: 123 439 569
Fragments arranged by size:
569 439 123
Bsp1286I G_dGCh'C
Cuts at: 0 689 826 1076 1131
Size: 689 137 250 55
Fragments arranged by size:
689 250 137 55
BspEI T'CCGG_A
Cuts at: 0 270 1131
Size: 270 861
BspHI T'CATG_A
Cuts at: 0 584 999 1131
Size: 584 415 132
Fragments arranged by size:
584 415 132
BsrDI GCAATG_nn'
Cuts at: 0 1074 1131
Size: 1074 57
BsrFI r'CCGG_y
Cuts at: 0 18 1055 1131
Size: 18 1037 76
Fragments arranged by size:
1037 76 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 547 777 1131
Size: 547 230 354
Fragments arranged by size:
547 354 230
BstYI r'GATC_y
Cuts at: 0 98 531 725 813 989 1131
Size: 98 433 194 88 176 142
Fragments arranged by size:
433 194 176 142 98 88
BstZ17I GTA'TAC
Cuts at: 0 753 1131
Size: 753 378
BtgI C'CryG_G
Cuts at: 0 188 540 1131
Size: 188 352 591
Fragments arranged by size:
591 352 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 272 1131
Size: 272 859
BtsI GCAGTG_nn'
Cuts at: 0 145 782 1131
Size: 145 637 349
Fragments arranged by size:
637 349 145
EaeI y'GGCC_r
Cuts at: 0 92 200 1057 1131
Size: 92 108 857 74
Fragments arranged by size:
857 108 92 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 1131
Size: 37 1094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 335 434 824 1131
Size: 220 115 99 390 307
Fragments arranged by size:
390 307 220 115 99
EcoNI CCTnn'n_nnAGG
Cuts at: 0 658 1131
Size: 658 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 981 1013 1131
Size: 430 32 519 32 118
Fragments arranged by size:
519 430 118 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1110 1131
Size: 1110 21
MscI TGG'CCA
Cuts at: 0 94 202 1131
Size: 94 108 929
Fragments arranged by size:
929 108 94
MslI CAynn'nnrTG
Cuts at: 0 215 545 589 1131
Size: 215 330 44 542
Fragments arranged by size:
542 330 215 44
MspA1I CmG'CkG
Cuts at: 0 364 789 1131
Size: 364 425 342
Fragments arranged by size:
425 364 342
NaeI GCC'GGC
Cuts at: 0 1057 1131
Size: 1057 74
NcoI C'CATG_G
Cuts at: 0 188 540 1131
Size: 188 352 591
Fragments arranged by size:
591 352 188
NdeI CA'TA_TG
Cuts at: 0 141 1017 1131
Size: 141 876 114
Fragments arranged by size:
876 141 114
NgoMIV G'CCGG_C
Cuts at: 0 1055 1131
Size: 1055 76
NspI r_CATG'y
Cuts at: 0 1126 1131
Size: 1126 5
PshAI GACnn'nnGTC
Cuts at: 0 958 1131
Size: 958 173
PstI C_TGCA'G
Cuts at: 0 230 1131
Size: 230 901
PvuII CAG'CTG
Cuts at: 0 364 789 1131
Size: 364 425 342
Fragments arranged by size:
425 364 342
SfcI C'TryA_G
Cuts at: 0 57 226 341 672 1131
Size: 57 169 115 331 459
Fragments arranged by size:
459 331 169 115 57
SmlI C'TyrA_G
Cuts at: 0 862 1131
Size: 862 269
SspI AAT'ATT
Cuts at: 0 485 718 1131
Size: 485 233 413
Fragments arranged by size:
485 413 233
StyI C'CwwG_G
Cuts at: 0 188 540 654 966 1131
Size: 188 352 114 312 165
Fragments arranged by size:
352 312 188 165 114
TatI w'GTAC_w
Cuts at: 0 738 866 1131
Size: 738 128 265
Fragments arranged by size:
738 265 128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 592 624 805 837 1131
Size: 592 32 181 32 294
Fragments arranged by size:
592 294 181 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 591 1131
Size: 591 540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 195 1131
Size: 195 936
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApoI AvaI AvrII
BaeI BamHI BanII BbsI BclI BglI BglII Bme1580I
BmgBI BpmI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI
BstXI BstYI BstZ17I BtgI BtgZI BtsI EaeI EciI
Eco57MI EcoNI Hin4I MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NspI PshAI PstI PvuII SfcI
SmlI SspI StyI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI BanI
BbeI BbvCI BcgI BciVI BfrBI BlpI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaHI BsiEI BsiWI BsmBI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
XbaI XhoI XmaI XmnI ZraI