(Circular) (Six-base) MAPSORT of: pFN21AB7630.seq Check: 2513 from: 1 to: 5830 pFN21AB7630 5830 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 2896 3151 2896 Size: 255 5575 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2476 3893 5277 5545 1632 Size: 482 362 1417 1384 268 1917 Fragments arranged by size: 1917 1417 1384 482 362 268 AflII C'TTAA_G Cuts at: 820 1017 2324 820 Size: 197 1307 4326 Fragments arranged by size: 4326 1307 197 AflIII A'CryG_T Cuts at: 1203 2762 4729 5706 1203 Size: 1559 1967 977 1327 Fragments arranged by size: 1967 1559 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 2420 1166 Size: 1254 4576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AlwNI CAG_nnn'CTG Cuts at: 3063 3573 5145 3063 Size: 510 1572 3748 Fragments arranged by size: 3748 1572 510 ApaI G_GGCC'C Cuts at: 2027 2027 Size: 5830 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 3139 3365 3517 5626 1493 Size: 1646 226 152 2109 1697 Fragments arranged by size: 2109 1697 1646 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 2642 3155 1870 Size: 73 699 513 4545 Fragments arranged by size: 4545 699 513 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2896 3151 4606 618 Size: 325 217 627 1109 255 1455 1842 Fragments arranged by size: 1842 1455 1109 627 325 255 217 BanII G_rGCy'C Cuts at: 1391 2027 2517 2649 2661 2760 2822 2867 2925 Size: 636 490 132 12 99 62 45 58 Cuts at: 2925 3149 1391 Size: 224 4072 Fragments arranged by size: 4072 636 490 224 132 99 62 58 45 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4859 BbvCI CC'TCA_GC Cuts at: 2687 2687 Size: 5830 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4021 4055 1903 Size: 34 50 34 2000 34 3678 Fragments arranged by size: 3678 2000 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2049 3722 4932 2049 Size: 1673 1210 2947 Fragments arranged by size: 2947 1673 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BfrBI ATG'CAT Cuts at: 2171 2542 2171 Size: 371 5459 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4441 136 Size: 107 122 71 631 605 2769 1525 Fragments arranged by size: 2769 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 2027 3891 5047 1790 Size: 237 1864 1156 2573 Fragments arranged by size: 2573 1864 1156 237 BmgBI CAC'GTC Cuts at: 1318 2484 2765 3114 1318 Size: 1166 281 349 4034 Fragments arranged by size: 4034 1166 349 281 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2983 3056 3561 Size: 416 53 83 399 24 1566 73 505 Cuts at: 3561 4519 442 Size: 958 1753 Fragments arranged by size: 1753 1566 958 505 416 399 83 73 53 24 BmtI G_CTAG'C Cuts at: 1056 2874 1056 Size: 1818 4012 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2246 2468 2872 2999 4490 1239 Size: 836 171 222 404 127 1491 2579 Fragments arranged by size: 2579 1491 836 404 222 171 127 Bpu10I CC'TnA_GC Cuts at: 2687 2687 Size: 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2690 3955 4820 5118 2690 Size: 1265 865 298 3402 Fragments arranged by size: 3402 1265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4493 882 Size: 617 2994 2219 Fragments arranged by size: 2994 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4019 4654 275 Size: 53 83 186 936 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2441 2453 2471 1178 Size: 30 165 30 1020 18 12 18 4537 Fragments arranged by size: 4537 1020 165 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 1186 Size: 973 4857 BseYI C'CCAG_C Cuts at: 2394 2774 2866 5033 5529 2394 Size: 380 92 2167 496 2695 Fragments arranged by size: 2695 2167 496 380 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3027 1163 Size: 573 1291 3966 Fragments arranged by size: 3966 1291 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2517 2661 2822 3891 3976 5047 2517 Size: 144 161 1069 85 1071 3300 Fragments arranged by size: 3300 1071 1069 161 144 85 BsmI GAATG_Cn' Cuts at: 2171 2342 3341 3434 2171 Size: 171 999 93 4567 Fragments arranged by size: 4567 999 171 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2517 2649 2661 2760 2822 2867 Size: 399 237 490 132 12 99 62 45 Cuts at: 2867 2925 3149 3891 3976 5047 1391 Size: 58 224 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 399 237 224 132 99 85 62 58 45 12 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2720 3185 844 Size: 1876 465 3489 Fragments arranged by size: 3489 1876 465 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2436 4474 4661 1700 Size: 110 626 2038 187 2869 Fragments arranged by size: 2869 2038 626 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3890 4902 3890 Size: 1012 4818 BstAPI GCAn_nnn'nTGC Cuts at: 794 2750 794 Size: 1956 3874 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 2389 1184 Size: 1205 4625 BstXI CCAn_nnnn'nTGG Cuts at: 2932 2932 Size: 5830 BstYI r'GATC_y Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825 Size: 1188 367 385 17 1452 86 12 346 Cuts at: 5825 1972 Size: 1977 Fragments arranged by size: 1977 1452 1188 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5278 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 3346 4140 4160 3346 Size: 794 20 5016 Fragments arranged by size: 5016 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DraIII CAC_nnn'GTG Cuts at: 2592 2592 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2254 3277 4166 Size: 54 1425 50 275 18 424 1023 889 Cuts at: 4166 5795 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1023 889 424 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2855 3760 1353 Size: 1502 905 3423 Fragments arranged by size: 3423 1502 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2031 2800 4417 4791 4937 387 Size: 1064 138 442 769 1617 374 146 1280 Fragments arranged by size: 1617 1280 1064 769 442 374 146 138 EcoICRI GAG'CTC Cuts at: 2515 2659 2820 2515 Size: 144 161 5525 Fragments arranged by size: 5525 161 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2114 2246 2468 2476 2872 2999 Size: 393 443 39 132 222 8 396 127 Cuts at: 2999 3893 4490 5277 5545 1239 Size: 894 597 787 268 1524 Fragments arranged by size: 1524 894 787 597 443 396 393 268 222 132 127 39 8 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 3054 4977 1678 Size: 1376 1923 2531 Fragments arranged by size: 2531 1923 1376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2502 2534 2551 2558 2583 Size: 32 135 32 930 32 17 7 25 Cuts at: 2583 2590 2648 2680 4471 4503 4545 4577 1373 Size: 7 58 32 1791 32 42 32 2626 Fragments arranged by size: 2626 1791 930 135 58 42 32 32 32 32 32 32 25 17 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2208 3174 3420 669 Size: 988 281 270 966 246 3079 Fragments arranged by size: 3079 988 966 281 270 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 2900 3155 2900 Size: 255 5575 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2544 2650 4944 5128 1305 Size: 1239 106 2294 184 2007 Fragments arranged by size: 2294 2007 1239 184 106 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4008 Fragments arranged by size: 4008 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2420 2930 3788 4147 4306 518 Size: 648 1254 510 858 359 159 2042 Fragments arranged by size: 2042 1254 858 648 510 359 159 MspA1I CmG'CkG Cuts at: 1606 3246 3923 5071 5316 1606 Size: 1640 677 1148 245 2120 Fragments arranged by size: 2120 1640 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5278 NdeI CA'TA_TG Cuts at: 387 2083 387 Size: 1696 4134 NheI G'CTAG_C Cuts at: 1052 2870 1052 Size: 1818 4012 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NsiI A_TGCA'T Cuts at: 2173 2544 2173 Size: 371 5459 NspI r_CATG'y Cuts at: 1132 1229 2546 3188 4733 1132 Size: 97 1317 642 1545 2229 Fragments arranged by size: 2229 1545 1317 642 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2066 2563 1108 Size: 958 497 4375 Fragments arranged by size: 4375 958 497 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PspOMI G'GGCC_C Cuts at: 2023 2023 Size: 5830 PspXI vC'TCGA_Gb Cuts at: 2642 2642 Size: 5830 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2435 2467 2435 Size: 32 5798 PstI C_TGCA'G Cuts at: 830 3182 830 Size: 2352 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 SacI G_AGCT'C Cuts at: 2517 2661 2822 2517 Size: 144 161 5525 Fragments arranged by size: 5525 161 144 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2855 2855 Size: 5830 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2101 2680 3178 4313 4994 5185 826 Size: 219 1056 579 498 1135 681 191 1471 Fragments arranged by size: 1471 1135 1056 681 579 498 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2324 2642 2705 3934 4835 5097 Size: 197 926 381 318 63 1229 901 262 Cuts at: 5097 820 Size: 1553 Fragments arranged by size: 1553 1229 926 901 381 318 262 197 63 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 2546 3188 1132 Size: 1414 642 3774 Fragments arranged by size: 3774 1414 642 SspI AAT'ATT Cuts at: 5 52 2109 3754 5 Size: 47 2057 1645 2081 Fragments arranged by size: 2081 2057 1645 47 StuI AGG'CCT Cuts at: 1517 3122 1517 Size: 1605 4225 StyI C'CwwG_G Cuts at: 513 1065 2334 3269 513 Size: 552 1269 935 3074 Fragments arranged by size: 3074 1269 935 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2432 3002 3857 3874 4027 4212 4657 Size: 1255 852 570 855 17 153 185 445 Cuts at: 4657 325 Size: 1498 Fragments arranged by size: 1498 1255 855 852 570 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2313 4076 5788 Size: 274 80 33 51 1443 336 1763 1712 Cuts at: 5788 96 Size: 138 Fragments arranged by size: 1763 1712 1443 336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2262 2294 5790 5822 2262 Size: 32 3496 32 2270 Fragments arranged by size: 3496 2270 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 2642 1943 Size: 699 5131 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI