(Circular) (Six-base) MAPSORT of: pFN21AB8390.seq Check: 3947 from: 1 to: 5899 pFN21AB8390 5899 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2161 2313 2534 2961 3051 2161 Size: 152 221 427 90 5009 Fragments arranged by size: 5009 427 221 152 90 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2936 3709 278 Size: 53 83 186 936 1400 773 2468 Fragments arranged by size: 2468 1400 936 773 186 83 53 AccI GT'mk_AC Cuts at: 1937 3242 1937 Size: 1305 4594 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2963 3962 5346 5614 1632 Size: 1331 999 1384 268 1917 Fragments arranged by size: 1917 1384 1331 999 268 AfeI AGC'GCT Cuts at: 3068 3095 3068 Size: 27 5872 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 2892 4798 5775 1203 Size: 1689 1906 977 1327 Fragments arranged by size: 1906 1689 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 3642 5214 3642 Size: 1572 4327 ApaI G_GGCC'C Cuts at: 2653 2735 2653 Size: 82 5817 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 3208 3434 3586 5695 1493 Size: 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 2401 2861 3080 3224 1870 Size: 73 458 460 219 144 4545 Fragments arranged by size: 4545 460 458 219 144 73 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2152 2173 2591 2636 2814 Size: 325 217 627 365 21 418 45 178 Cuts at: 2814 2855 3039 3220 4675 618 Size: 41 184 181 1455 1842 Fragments arranged by size: 1842 1455 627 418 365 325 217 184 181 178 45 41 21 BanII G_rGCy'C Cuts at: 1391 2198 2653 2735 3218 1391 Size: 807 455 82 483 4072 Fragments arranged by size: 4072 807 483 455 82 BbeI G_GCGC'C Cuts at: 2156 2595 2640 2859 2156 Size: 439 45 219 5196 Fragments arranged by size: 5196 439 219 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2303 2936 928 Size: 971 404 633 3891 Fragments arranged by size: 3891 971 633 404 BbvCI CC'TCA_GC Cuts at: 3162 3162 Size: 5899 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2133 2142 Size: 107 122 71 631 605 343 118 9 Cuts at: 2142 2634 2681 2795 2864 4510 136 Size: 492 47 114 69 1646 1525 Fragments arranged by size: 1646 1525 631 605 492 343 122 118 114 107 71 69 47 9 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 3316 3316 Size: 5899 Bme1580I G_kGCm'C Cuts at: 1790 2178 2448 2653 2735 3960 5116 1790 Size: 388 270 205 82 1225 1156 2573 Fragments arranged by size: 2573 1225 1156 388 270 205 82 BmgBI CAC'GTC Cuts at: 1318 3127 1318 Size: 1809 4090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3630 4588 442 Size: 416 53 83 399 24 2213 958 1753 Fragments arranged by size: 2213 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2188 4559 1239 Size: 797 152 2371 2579 Fragments arranged by size: 2579 2371 797 152 Bpu10I CC'TnA_GC Cuts at: 3162 3162 Size: 5899 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4024 4889 5187 4024 Size: 865 298 4736 Fragments arranged by size: 4736 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4562 882 Size: 617 3063 2219 Fragments arranged by size: 3063 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2443 2893 2902 3690 493 Size: 711 1239 450 9 788 2702 Fragments arranged by size: 2702 1239 788 711 450 9 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2153 2288 2592 2637 Size: 53 83 186 936 620 135 304 45 Cuts at: 2637 2856 2933 3706 4088 4723 275 Size: 219 77 773 382 635 1451 Fragments arranged by size: 1451 936 773 635 620 382 304 219 186 135 83 77 53 45 BsaWI w'CCGG_w Cuts at: 2025 4332 4730 5004 5151 5562 2025 Size: 2307 398 274 147 411 2362 Fragments arranged by size: 2362 2307 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5674 Fragments arranged by size: 5674 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5899 BseYI C'CCAG_C Cuts at: 2066 2191 2273 5102 5598 2066 Size: 125 82 2829 496 2367 Fragments arranged by size: 2829 2367 496 125 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5326 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2685 2700 3349 4110 5138 1540 Size: 275 186 684 15 649 761 1028 2301 Fragments arranged by size: 2301 1028 761 684 649 275 186 15 BsiHKAI G_wGCw'C Cuts at: 3960 4045 5116 3960 Size: 85 1071 4743 Fragments arranged by size: 4743 1071 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 2198 2448 2653 2735 3218 3960 Size: 399 388 20 250 205 82 483 742 Cuts at: 3960 4045 5116 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 483 399 388 250 205 85 82 20 BspEI T'CCGG_A Cuts at: 2025 5562 2025 Size: 3537 2362 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2161 2313 2496 2534 2961 3051 3254 844 Size: 1317 152 183 38 427 90 203 3489 Fragments arranged by size: 3489 1317 427 203 183 152 90 38 BsrBI CCG'CTC Cuts at: 1394 1823 3786 1394 Size: 429 1963 3507 Fragments arranged by size: 3507 1963 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2105 4394 4568 65 Size: 1183 857 2289 174 1396 Fragments arranged by size: 2289 1396 1183 857 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2236 2383 4543 4730 1700 Size: 110 426 147 2160 187 2869 Fragments arranged by size: 2869 2160 426 187 147 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 2430 1925 Size: 505 5394 BssSI C'ACGA_G Cuts at: 2330 3959 4971 2330 Size: 1629 1012 3258 Fragments arranged by size: 3258 1629 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5899 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstYI r'GATC_y Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894 Size: 1257 367 385 17 1452 86 12 346 Cuts at: 5894 1972 Size: 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2219 2567 2802 513 Size: 552 939 6 209 348 235 3610 Fragments arranged by size: 3610 939 552 348 235 209 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5160 Fragments arranged by size: 5160 683 56 BtsI GCAGTG_nn' Cuts at: 2255 3415 4209 4229 2255 Size: 1160 794 20 3925 Fragments arranged by size: 3925 1160 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2016 2112 Size: 54 1425 50 275 18 177 9 96 Cuts at: 2112 2134 2682 2697 2799 2805 3346 4235 5864 Size: 22 548 15 102 6 541 889 1629 Cuts at: 5864 8 Size: 43 Fragments arranged by size: 1629 1425 889 548 541 275 177 102 96 54 50 43 22 18 15 9 6 EagI C'GGCC_G Cuts at: 1537 1812 2682 2697 3346 1537 Size: 275 870 15 649 4090 Fragments arranged by size: 4090 870 649 275 15 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 3002 3829 1353 Size: 863 786 827 3423 Fragments arranged by size: 3423 863 827 786 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2487 2882 4486 4860 5006 387 Size: 1064 138 898 395 1604 374 146 1280 Fragments arranged by size: 1604 1280 1064 898 395 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2188 2963 3962 4559 5346 5614 Size: 393 404 152 775 999 597 787 268 Cuts at: 5614 1239 Size: 1524 Fragments arranged by size: 1524 999 787 775 597 404 393 268 152 EcoO109I rG'GnC_Cy Cuts at: 2604 2649 2650 2788 3193 2604 Size: 45 1 138 405 5310 Fragments arranged by size: 5310 405 138 45 1 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 FspI TGC'GCA Cuts at: 2328 2748 3104 4405 2328 Size: 420 356 1301 3822 Fragments arranged by size: 3822 1301 420 356 HaeII r_GCGC'y Cuts at: 1678 2073 2156 2595 2640 2859 3070 3097 5046 Size: 395 83 439 45 219 211 27 1949 Cuts at: 5046 1678 Size: 2531 Fragments arranged by size: 2531 1949 439 395 219 211 83 45 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4540 4572 4614 4646 1373 Size: 32 135 32 2968 32 42 32 2626 Fragments arranged by size: 2968 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3243 3489 669 Size: 988 281 1305 246 3079 Fragments arranged by size: 3079 1305 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KasI G'GCGC_C Cuts at: 2152 2591 2636 2855 2152 Size: 439 45 219 5196 Fragments arranged by size: 5196 439 219 45 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2508 2718 5013 5197 1305 Size: 1203 210 2295 184 2007 Fragments arranged by size: 2295 2007 1203 210 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4077 Fragments arranged by size: 4077 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3857 4216 4375 518 Size: 648 2691 359 159 2042 Fragments arranged by size: 2691 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2427 2687 2823 2829 3037 3315 3992 Size: 406 415 260 136 6 208 278 677 Cuts at: 3992 5140 5385 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 415 406 278 260 245 208 136 6 NaeI GCC'GGC Cuts at: 2238 2238 Size: 5899 NarI GG'CG_CC Cuts at: 2153 2592 2637 2856 2153 Size: 439 45 219 5196 Fragments arranged by size: 5196 439 219 45 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4408 Fragments arranged by size: 4408 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NgoMIV G'CCGG_C Cuts at: 2236 2236 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 2682 2697 3346 2682 Size: 15 649 5235 Fragments arranged by size: 5235 649 15 NruI TCG'CGA Cuts at: 3119 3119 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 2236 3257 4802 1132 Size: 97 1007 1021 1545 2229 Fragments arranged by size: 2229 1545 1021 1007 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5899 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5899 PfoI T'CCnGG_A Cuts at: 2052 2617 2052 Size: 565 5334 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PmlI CAC'GTG Cuts at: 2893 2902 2893 Size: 9 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3193 3193 Size: 5899 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspOMI G'GGCC_C Cuts at: 2649 2731 2649 Size: 82 5817 PspXI vC'TCGA_Gb Cuts at: 2401 2401 Size: 5899 PstI C_TGCA'G Cuts at: 830 2043 2172 2310 2781 3036 3251 830 Size: 1213 129 138 471 255 215 3478 Fragments arranged by size: 3478 1213 471 255 215 138 129 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 PvuII CAG'CTG Cuts at: 2427 2427 Size: 5899 SacII CC_GC'GG Cuts at: 2013 2013 Size: 5899 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SapI GCTCTTCn'nnn_ Cuts at: 2216 3002 2216 Size: 786 5113 SbfI CC_TGCA'GG Cuts at: 2172 3251 2172 Size: 1079 4820 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SexAI A'CCwGG_T Cuts at: 3195 3195 Size: 5899 SfcI C'TryA_G Cuts at: 826 1045 2039 2122 2168 2306 2777 3032 3247 Size: 219 994 83 46 138 471 255 215 Cuts at: 3247 4382 5063 5254 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 994 681 471 255 219 215 191 138 83 46 SfiI GGCCn_nnn'nGGCC Cuts at: 2015 2015 Size: 5899 SfoI GGC'GCC Cuts at: 2154 2593 2638 2857 2154 Size: 439 45 219 5196 Fragments arranged by size: 5196 439 219 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 3226 1872 Size: 1354 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2401 4003 4904 5166 820 Size: 197 926 458 1602 901 262 1553 Fragments arranged by size: 1602 1553 926 901 458 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 2045 2721 1517 Size: 528 676 4695 Fragments arranged by size: 4695 676 528 StyI C'CwwG_G Cuts at: 513 1065 2004 2784 3338 513 Size: 552 939 780 554 3074 Fragments arranged by size: 3074 939 780 554 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2557 3926 3943 4096 4281 4726 325 Size: 1255 977 1369 17 153 185 445 1498 Fragments arranged by size: 1498 1369 1255 977 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4145 5857 96 Size: 274 80 33 51 1443 2168 1712 138 Fragments arranged by size: 2168 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5859 5891 5859 Size: 32 5867 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2791 2011 Size: 780 5119 XhoI C'TCGA_G Cuts at: 1943 2401 1943 Size: 458 5441 XmaI C'CCGG_G Cuts at: 1870 3224 1870 Size: 1354 4545 XmnI GAAnn'nnTTC Cuts at: 3011 4028 3011 Size: 1017 4882 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2934 3707 276 Size: 53 83 186 936 1400 773 2468 Fragments arranged by size: 2468 1400 936 773 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NsiI PacI PsrI RsrII SacI SanDI SrfI SwaI