Restriction map for
pFN21AB8390
(Linear) (Six-base) MAPSORT of: KIBB8390.seq Check: 2821 from: 1 to: 1194
KIBB8390.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 156 308 529 956 1046 1194
Size: 156 152 221 427 90 148
Fragments arranged by size:
427 221 156 152 148 90
AatII G_ACGT'C
Cuts at: 0 931 1194
Size: 931 263
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1194
Size: 958 236
AfeI AGC'GCT
Cuts at: 0 1063 1090 1194
Size: 1063 27 104
Fragments arranged by size:
1063 104 27
AflIII A'CryG_T
Cuts at: 0 887 1194
Size: 887 307
ApaI G_GGCC'C
Cuts at: 0 648 730 1194
Size: 648 82 464
Fragments arranged by size:
648 464 82
AvaI C'yCGr_G
Cuts at: 0 396 856 1075 1194
Size: 396 460 219 119
Fragments arranged by size:
460 396 219 119
BanI G'GyrC_C
Cuts at: 0 147 168 586 631 809 850 1034 1194
Size: 147 21 418 45 178 41 184 160
Fragments arranged by size:
418 184 178 160 147 45 41 21
BanII G_rGCy'C
Cuts at: 0 193 648 730 1194
Size: 193 455 82 464
Fragments arranged by size:
464 455 193 82
BbeI G_GCGC'C
Cuts at: 0 151 590 635 854 1194
Size: 151 439 45 219 340
Fragments arranged by size:
439 340 219 151 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 298 931 1194
Size: 298 633 263
Fragments arranged by size:
633 298 263
BbvCI CC'TCA_GC
Cuts at: 0 1157 1194
Size: 1157 37
BglI GCCn_nnn'nGGC
Cuts at: 0 10 128 137 629 676 790 859 1194
Size: 10 118 9 492 47 114 69 335
Fragments arranged by size:
492 335 118 114 69 47 10 9
Bme1580I G_kGCm'C
Cuts at: 0 173 443 648 730 1194
Size: 173 270 205 82 464
Fragments arranged by size:
464 270 205 173 82
BmgBI CAC'GTC
Cuts at: 0 1122 1194
Size: 1122 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 183 1194
Size: 31 152 1011
Fragments arranged by size:
1011 152 31
Bpu10I CC'TnA_GC
Cuts at: 0 1157 1194
Size: 1157 37
BsaAI yAC'GTr
Cuts at: 0 438 888 897 1194
Size: 438 450 9 297
Fragments arranged by size:
450 438 297 9
BsaHI Gr'CG_yC
Cuts at: 0 148 283 587 632 851 928 1194
Size: 148 135 304 45 219 77 266
Fragments arranged by size:
304 266 219 148 135 77 45
BsaWI w'CCGG_w
Cuts at: 0 20 1194
Size: 20 1174
BseYI C'CCAG_C
Cuts at: 0 61 186 268 1194
Size: 61 125 82 926
Fragments arranged by size:
926 125 82 61
BsiEI CG_ry'CG
Cuts at: 0 680 695 1194
Size: 680 15 499
Fragments arranged by size:
680 499 15
Bsp1286I G_dGCh'C
Cuts at: 0 173 193 443 648 730 1194
Size: 173 20 250 205 82 464
Fragments arranged by size:
464 250 205 173 82 20
BspEI T'CCGG_A
Cuts at: 0 20 1194
Size: 20 1174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 156 308 491 529 956 1046 1194
Size: 156 152 183 38 427 90 148
Fragments arranged by size:
427 183 156 152 148 90 38
BsrDI GCAATG_nn'
Cuts at: 0 100 1194
Size: 100 1094
BsrFI r'CCGG_y
Cuts at: 0 231 378 1194
Size: 231 147 816
Fragments arranged by size:
816 231 147
BssHII G'CGCG_C
Cuts at: 0 425 1194
Size: 425 769
BssSI C'ACGA_G
Cuts at: 0 325 1194
Size: 325 869
BtgI C'CryG_G
Cuts at: 0 5 214 562 797 1194
Size: 5 209 348 235 397
Fragments arranged by size:
397 348 235 209 5
BtsI GCAGTG_nn'
Cuts at: 0 250 1194
Size: 250 944
EaeI y'GGCC_r
Cuts at: 0 2 11 107 129 677 692 794 800
Size: 2 9 96 22 548 15 102 6
Cuts at: 800 1194
Size: 394
Fragments arranged by size:
548 394 102 96 22 15 9 6
2
EagI C'GGCC_G
Cuts at: 0 677 692 1194
Size: 677 15 502
Fragments arranged by size:
677 502 15
EarI CTCTTCn'nnn_
Cuts at: 0 211 997 1194
Size: 211 786 197
Fragments arranged by size:
786 211 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 482 877 1194
Size: 482 395 317
Fragments arranged by size:
482 395 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 183 958 1194
Size: 31 152 775 236
Fragments arranged by size:
775 236 152 31
EcoO109I rG'GnC_Cy
Cuts at: 0 599 644 645 783 1188 1194
Size: 599 45 1 138 405 6
Fragments arranged by size:
599 405 138 45 6 1
FspI TGC'GCA
Cuts at: 0 323 743 1099 1194
Size: 323 420 356 95
Fragments arranged by size:
420 356 323 95
HaeII r_GCGC'y
Cuts at: 0 68 151 590 635 854 1065 1092 1194
Size: 68 83 439 45 219 211 27 102
Fragments arranged by size:
439 219 211 102 83 68 45 27
KasI G'GCGC_C
Cuts at: 0 147 586 631 850 1194
Size: 147 439 45 219 344
Fragments arranged by size:
439 344 219 147 45
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 713 1194
Size: 503 210 481
Fragments arranged by size:
503 481 210
MspA1I CmG'CkG
Cuts at: 0 7 422 682 818 824 1032 1194
Size: 7 415 260 136 6 208 162
Fragments arranged by size:
415 260 208 162 136 7 6
NaeI GCC'GGC
Cuts at: 0 233 1194
Size: 233 961
NarI GG'CG_CC
Cuts at: 0 148 587 632 851 1194
Size: 148 439 45 219 343
Fragments arranged by size:
439 343 219 148 45
NgoMIV G'CCGG_C
Cuts at: 0 231 1194
Size: 231 963
NotI GC'GGCC_GC
Cuts at: 0 677 692 1194
Size: 677 15 502
Fragments arranged by size:
677 502 15
NruI TCG'CGA
Cuts at: 0 1114 1194
Size: 1114 80
NspI r_CATG'y
Cuts at: 0 231 1194
Size: 231 963
PfoI T'CCnGG_A
Cuts at: 0 47 612 1194
Size: 47 565 582
Fragments arranged by size:
582 565 47
PmlI CAC'GTG
Cuts at: 0 888 897 1194
Size: 888 9 297
Fragments arranged by size:
888 297 9
PpuMI rG'GwC_Cy
Cuts at: 0 1188 1194
Size: 1188 6
PspOMI G'GGCC_C
Cuts at: 0 644 726 1194
Size: 644 82 468
Fragments arranged by size:
644 468 82
PspXI vC'TCGA_Gb
Cuts at: 0 396 1194
Size: 396 798
PstI C_TGCA'G
Cuts at: 0 38 167 305 776 1031 1194
Size: 38 129 138 471 255 163
Fragments arranged by size:
471 255 163 138 129 38
PvuII CAG'CTG
Cuts at: 0 422 1194
Size: 422 772
SacII CC_GC'GG
Cuts at: 0 8 1194
Size: 8 1186
SapI GCTCTTCn'nnn_
Cuts at: 0 211 997 1194
Size: 211 786 197
Fragments arranged by size:
786 211 197
SbfI CC_TGCA'GG
Cuts at: 0 167 1194
Size: 167 1027
SfcI C'TryA_G
Cuts at: 0 34 117 163 301 772 1027 1194
Size: 34 83 46 138 471 255 167
Fragments arranged by size:
471 255 167 138 83 46 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 10 1194
Size: 10 1184
SfoI GGC'GCC
Cuts at: 0 149 588 633 852 1194
Size: 149 439 45 219 342
Fragments arranged by size:
439 342 219 149 45
SmlI C'TyrA_G
Cuts at: 0 396 1194
Size: 396 798
StuI AGG'CCT
Cuts at: 0 40 716 1194
Size: 40 676 478
Fragments arranged by size:
676 478 40
StyI C'CwwG_G
Cuts at: 0 779 1194
Size: 779 415
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 552 1194
Size: 552 642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 786 1194
Size: 786 408
XhoI C'TCGA_G
Cuts at: 0 396 1194
Size: 396 798
XmnI GAAnn'nnTTC
Cuts at: 0 1006 1194
Size: 1006 188
ZraI GAC'GTC
Cuts at: 0 929 1194
Size: 929 265
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII ApaI AvaI BanI
BanII BbeI BbsI BbvCI BglI Bme1580I BmgBI BpmI
Bpu10I BsaAI BsaHI BsaWI BseYI BsiEI Bsp1286I BspEI
BspMI BsrDI BsrFI BssHII BssSI BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I FspI HaeII KasI
MmeI MspA1I NaeI NarI NgoMIV NotI NruI NspI
PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacII
SapI SbfI SfcI SfiI SfoI SmlI StuI StyI
TaqII XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI BpuEI BsaI BsaBI BsaXI BseRI
BsgI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrGI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NdeI NheI
NsiI PacI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PvuI RsrII SacI SalI SanDI ScaI
SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XmaI