(Circular) (Six-base) MAPSORT of: pFN21AB8377.seq Check: 6354 from: 1 to: 6571 pFN21AB8377 6571 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2234 3051 3381 3426 3672 2234 Size: 817 330 45 246 5133 Fragments arranged by size: 5133 817 330 246 45 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3914 1937 Size: 1977 4594 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3362 3653 3698 4634 6018 6286 1632 Size: 1730 291 45 936 1384 268 1917 Fragments arranged by size: 1917 1730 1384 936 291 268 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 5470 6447 1203 Size: 4267 977 1327 Fragments arranged by size: 4267 1327 977 AgeI A'CCGG_T Cuts at: 5402 5402 Size: 6571 AhdI GACnn_n'nnGTC Cuts at: 5300 5300 Size: 6571 AleI CACnn'nnGTG Cuts at: 1166 2024 3715 1166 Size: 858 1691 4022 Fragments arranged by size: 4022 1691 858 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3079 1184 Size: 1895 4676 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3131 3163 3131 Size: 32 6539 AlwNI CAG_nnn'CTG Cuts at: 2662 2881 3676 4314 5886 2662 Size: 219 795 638 1572 3347 Fragments arranged by size: 3347 1572 795 638 219 ApaI G_GGCC'C Cuts at: 2340 3177 2340 Size: 837 5734 ApaLI G'TGCA_C Cuts at: 4628 5784 4628 Size: 1156 5415 ApoI r'AATT_y Cuts at: 1493 3428 3836 3880 4106 4258 6367 1493 Size: 1935 408 44 226 152 2109 1697 Fragments arranged by size: 2109 1935 1697 408 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 3443 3896 1870 Size: 73 1500 453 4545 Fragments arranged by size: 4545 1500 453 73 BamHI G'GATC_C Cuts at: 3801 3901 4268 3801 Size: 100 367 6104 Fragments arranged by size: 6104 367 100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3063 3404 3438 3892 5347 Size: 325 217 627 1276 341 34 454 1455 Cuts at: 5347 618 Size: 1842 Fragments arranged by size: 1842 1455 1276 627 454 341 325 217 34 BanII G_rGCy'C Cuts at: 1391 2033 2154 2163 2184 2340 2358 3177 3235 Size: 642 121 9 21 156 18 819 58 Cuts at: 3235 3890 1391 Size: 655 4072 Fragments arranged by size: 4072 819 655 642 156 121 58 21 18 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5600 BbvCI CC'TCA_GC Cuts at: 2568 2568 Size: 6571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3424 3458 3521 3555 4762 4796 1903 Size: 34 1487 34 63 34 1207 34 3678 Fragments arranged by size: 3678 1487 1207 63 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3586 4463 5673 3586 Size: 877 1210 4484 Fragments arranged by size: 4484 1210 877 BclI T'GATC_A Cuts at: 1548 2664 1548 Size: 1116 5455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 2433 5182 Size: 107 122 71 631 605 418 343 2749 Cuts at: 5182 136 Size: 1525 Fragments arranged by size: 2749 1525 631 605 418 343 122 107 71 BglII A'GATC_T Cuts at: 6566 6566 Size: 6571 BlpI GC'TnA_GC Cuts at: 3266 3988 3266 Size: 722 5849 Bme1580I G_kGCm'C Cuts at: 1790 2340 3177 3409 3638 4632 5788 1790 Size: 550 837 232 229 994 1156 2573 Fragments arranged by size: 2573 1156 994 837 550 232 229 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6571 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2768 2891 3410 Size: 416 53 83 399 24 1351 123 519 Cuts at: 3410 4302 5260 442 Size: 892 958 1753 Fragments arranged by size: 1753 1351 958 892 519 416 399 123 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2180 2180 Size: 6571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2017 2336 2639 2876 2924 2933 3153 3746 Size: 778 319 303 237 48 9 220 593 Cuts at: 3746 5231 1239 Size: 1485 2579 Fragments arranged by size: 2579 1485 778 593 319 303 237 220 48 9 Bpu10I CC'TnA_GC Cuts at: 2133 2357 2568 2133 Size: 224 211 6136 Fragments arranged by size: 6136 224 211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4696 5561 5859 4696 Size: 865 298 5408 Fragments arranged by size: 5408 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2489 2657 3364 3771 5234 882 Size: 617 990 168 707 407 1463 2219 Fragments arranged by size: 2219 1463 990 707 617 407 168 BsaAI yAC'GTr Cuts at: 493 1204 4362 493 Size: 711 3158 2702 Fragments arranged by size: 3158 2702 711 BsaBI GATnn'nnATC Cuts at: 6565 6565 Size: 6571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3822 4378 4760 5395 Size: 53 83 186 936 2289 556 382 635 Cuts at: 5395 275 Size: 1451 Fragments arranged by size: 2289 1451 936 635 556 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2102 5004 5402 5676 5823 6234 2102 Size: 2902 398 274 147 411 2439 Fragments arranged by size: 2902 2439 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 3131 3161 1178 Size: 30 165 30 993 30 705 30 4588 Fragments arranged by size: 4588 993 705 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 3272 3608 1186 Size: 967 1119 336 4149 Fragments arranged by size: 4149 1119 967 336 BseYI C'CCAG_C Cuts at: 2563 5774 6270 2563 Size: 3211 496 2864 Fragments arranged by size: 3211 2864 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2405 3647 3693 1163 Size: 573 669 1242 46 4041 Fragments arranged by size: 4041 1242 669 573 46 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4021 4782 5810 1540 Size: 275 186 2020 761 1028 2301 Fragments arranged by size: 2301 2020 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2800 4632 4717 5788 2800 Size: 1832 85 1071 3583 Fragments arranged by size: 3583 1832 1071 85 BsmI GAATG_Cn' Cuts at: 4082 4175 4082 Size: 93 6478 BsmBI CGTCTCn'nnnn_ Cuts at: 3081 3081 Size: 6571 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2154 2163 2184 2340 2358 2800 Size: 399 243 121 9 21 156 18 442 Cuts at: 2800 3177 3235 3409 3638 3890 4632 4717 5788 Size: 377 58 174 229 252 742 85 1071 Cuts at: 5788 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 442 399 377 252 243 229 174 156 121 85 58 21 18 9 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 2701 4460 6190 2701 Size: 1759 1730 3082 Fragments arranged by size: 3082 1759 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2234 3051 3120 3381 3426 3672 3926 844 Size: 1390 817 69 261 45 246 254 3489 Fragments arranged by size: 3489 1390 817 261 254 246 69 45 BsrBI CCG'CTC Cuts at: 1394 1823 4458 1394 Size: 429 2635 3507 Fragments arranged by size: 3507 2635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5066 5240 65 Size: 1183 3818 174 1396 Fragments arranged by size: 3818 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 5215 5402 1700 Size: 110 257 3148 187 2869 Fragments arranged by size: 3148 2869 257 187 110 BsrGI T'GTAC_A Cuts at: 96 2748 96 Size: 2652 3919 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 4631 5643 4631 Size: 1012 5559 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6571 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6571 BstXI CCAn_nnnn'nTGG Cuts at: 2026 2041 2026 Size: 15 6556 BstYI r'GATC_y Cuts at: 1972 2530 3801 3901 4268 4653 4670 6122 6208 Size: 558 1271 100 367 385 17 1452 86 Cuts at: 6208 6220 6566 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1271 558 385 367 346 100 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2243 2713 3186 3468 2243 Size: 470 473 282 5346 Fragments arranged by size: 5346 473 470 282 BtgI C'CryG_G Cuts at: 513 1065 2726 513 Size: 552 1661 4358 Fragments arranged by size: 4358 1661 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5832 Fragments arranged by size: 5832 683 56 BtsI GCAGTG_nn' Cuts at: 3527 3705 4087 4881 4901 3527 Size: 178 382 794 20 5197 Fragments arranged by size: 5197 794 382 178 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6536 DraI TTT'AAA Cuts at: 3875 3875 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3586 4018 4907 Size: 54 1425 50 275 18 1756 432 889 Cuts at: 4907 6536 8 Size: 1629 43 Fragments arranged by size: 1756 1629 1425 889 432 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4018 1537 Size: 275 2206 4090 Fragments arranged by size: 4090 2206 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3458 3674 4501 1353 Size: 2105 216 827 3423 Fragments arranged by size: 3423 2105 827 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5158 5532 5678 387 Size: 1064 138 3569 374 146 1280 Fragments arranged by size: 3569 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2017 2336 2639 2876 2924 2933 3153 Size: 393 385 319 303 237 48 9 220 Cuts at: 3153 3362 3653 3698 3746 4634 5231 6018 6286 Size: 209 291 45 48 888 597 787 268 Cuts at: 6286 1239 Size: 1524 Fragments arranged by size: 1524 888 787 597 393 385 319 303 291 268 237 220 209 48 48 45 9 EcoNI CCTnn'n_nnAGG Cuts at: 2825 3377 2825 Size: 552 6019 EcoO109I rG'GnC_Cy Cuts at: 2336 2476 2763 2843 3173 3174 2336 Size: 140 287 80 330 1 5733 Fragments arranged by size: 5733 330 287 140 80 1 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 EcoRV GAT'ATC Cuts at: 2290 2290 Size: 6571 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 5718 1678 Size: 4040 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2396 2428 2770 2802 2993 Size: 32 135 32 824 32 342 32 191 Cuts at: 2993 3025 3673 3705 5212 5244 5286 5318 1373 Size: 32 648 32 1507 32 42 32 2626 Fragments arranged by size: 2626 1507 824 648 342 191 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3915 4161 669 Size: 988 281 1977 246 3079 Fragments arranged by size: 3079 1977 988 281 246 HindIII A'AGCT_T Cuts at: 748 2251 748 Size: 1503 5068 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2325 5685 5869 1305 Size: 1020 3360 184 2007 Fragments arranged by size: 3360 2007 1020 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4749 Fragments arranged by size: 4749 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 3715 3753 4529 4888 5047 518 Size: 648 858 1691 38 776 359 159 2042 Fragments arranged by size: 2042 1691 858 776 648 359 159 38 MspA1I CmG'CkG Cuts at: 1606 2037 2192 2296 2567 3206 3585 3987 4664 Size: 431 155 104 271 639 379 402 677 Cuts at: 4664 5812 6057 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 639 431 402 379 271 245 155 104 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6019 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 3929 5474 1132 Size: 97 2700 1545 2229 Fragments arranged by size: 2700 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2260 2605 1348 Size: 912 345 5314 Fragments arranged by size: 5314 912 345 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 3415 1108 Size: 2307 4264 PfoI T'CCnGG_A Cuts at: 2655 2655 Size: 6571 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2340 2372 3131 3163 4640 4672 6153 6185 2340 Size: 32 759 32 1477 32 1481 32 2726 Fragments arranged by size: 2726 1481 1477 759 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 2763 2843 2476 Size: 287 80 6204 Fragments arranged by size: 6204 287 80 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PspOMI G'GGCC_C Cuts at: 2336 3173 2336 Size: 837 5734 PspXI vC'TCGA_Gb Cuts at: 3443 3443 Size: 6571 PstI C_TGCA'G Cuts at: 830 2191 2442 2598 3097 3534 3923 830 Size: 1361 251 156 499 437 389 3478 Fragments arranged by size: 3478 1361 499 437 389 251 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 PvuII CAG'CTG Cuts at: 2037 2192 2567 2037 Size: 155 375 6041 Fragments arranged by size: 6041 375 155 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SapI GCTCTTCn'nnn_ Cuts at: 3458 3458 Size: 6571 SbfI CC_TGCA'GG Cuts at: 2442 2598 3923 2442 Size: 156 1325 5090 Fragments arranged by size: 5090 1325 156 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SexAI A'CCwGG_T Cuts at: 3867 3867 Size: 6571 SfcI C'TryA_G Cuts at: 826 1045 2187 2438 2594 3068 3093 3530 3919 Size: 219 1142 251 156 474 25 437 389 Cuts at: 3919 5054 5735 5926 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1142 1135 681 474 437 389 251 219 191 156 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 3898 1872 Size: 2026 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3443 4675 5576 5838 820 Size: 197 926 1500 1232 901 262 1553 Fragments arranged by size: 1553 1500 1232 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3929 1132 Size: 2797 3774 SspI AAT'ATT Cuts at: 5 52 4495 5 Size: 47 4443 2081 Fragments arranged by size: 4443 2081 47 StuI AGG'CCT Cuts at: 1517 2718 2861 2930 3389 1517 Size: 1201 143 69 459 4699 Fragments arranged by size: 4699 1201 459 143 69 StyI C'CwwG_G Cuts at: 513 1065 2085 2208 2647 3143 3330 4010 513 Size: 552 1020 123 439 496 187 680 3074 Fragments arranged by size: 3074 1020 680 552 496 439 187 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3383 3392 4598 4615 4768 4953 5398 Size: 1255 1803 9 1206 17 153 185 445 Cuts at: 5398 325 Size: 1498 Fragments arranged by size: 1803 1498 1255 1206 445 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2748 2805 4817 Size: 274 80 33 51 1443 771 57 2012 Cuts at: 4817 6529 96 Size: 1712 138 Fragments arranged by size: 2012 1712 1443 771 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6531 6563 6531 Size: 32 6539 Tth111I GACn'n_nGTC Cuts at: 1316 2375 1316 Size: 1059 5512 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 3443 1943 Size: 1500 5071 XmaI C'CCGG_G Cuts at: 1870 3896 1870 Size: 2026 4545 XmnI GAAnn'nnTTC Cuts at: 3837 4700 3837 Size: 863 5708 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI