Restriction map for
pFN21AB8377
(Linear) (Six-base) MAPSORT of: KIBB8377.seq Check: 6669 from: 1 to: 1866
KIBB8377.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 229 1046 1376 1421 1667 1866
Size: 229 817 330 45 246 199
Fragments arranged by size:
817 330 246 229 199 45
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1357 1648 1693 1866
Size: 1357 291 45 173
Fragments arranged by size:
1357 291 173 45
AleI CACnn'nnGTG
Cuts at: 0 19 1710 1866
Size: 19 1691 156
Fragments arranged by size:
1691 156 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1074 1866
Size: 1074 792
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1126 1158 1866
Size: 1126 32 708
Fragments arranged by size:
1126 708 32
AlwNI CAG_nnn'CTG
Cuts at: 0 657 876 1671 1866
Size: 657 219 795 195
Fragments arranged by size:
795 657 219 195
ApaI G_GGCC'C
Cuts at: 0 335 1172 1866
Size: 335 837 694
Fragments arranged by size:
837 694 335
ApoI r'AATT_y
Cuts at: 0 1423 1831 1866
Size: 1423 408 35
Fragments arranged by size:
1423 408 35
AvaI C'yCGr_G
Cuts at: 0 1438 1866
Size: 1438 428
BamHI G'GATC_C
Cuts at: 0 1796 1866
Size: 1796 70
BanI G'GyrC_C
Cuts at: 0 1058 1399 1433 1866
Size: 1058 341 34 433
Fragments arranged by size:
1058 433 341 34
BanII G_rGCy'C
Cuts at: 0 28 149 158 179 335 353 1172 1230
Size: 28 121 9 21 156 18 819 58
Cuts at: 1230 1866
Size: 636
Fragments arranged by size:
819 636 156 121 58 28 21 18
9
BbvCI CC'TCA_GC
Cuts at: 0 563 1866
Size: 563 1303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1419 1453 1516 1550 1866
Size: 1419 34 63 34 316
Fragments arranged by size:
1419 316 63 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1581 1866
Size: 1581 285
BclI T'GATC_A
Cuts at: 0 659 1866
Size: 659 1207
BglI GCCn_nnn'nGGC
Cuts at: 0 85 428 1866
Size: 85 343 1438
Fragments arranged by size:
1438 343 85
BlpI GC'TnA_GC
Cuts at: 0 1261 1866
Size: 1261 605
Bme1580I G_kGCm'C
Cuts at: 0 335 1172 1404 1633 1866
Size: 335 837 232 229 233
Fragments arranged by size:
837 335 233 232 229
BmrI ACTGGGnnnn_n'
Cuts at: 0 763 886 1405 1866
Size: 763 123 519 461
Fragments arranged by size:
763 519 461 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 175 1866
Size: 175 1691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 331 634 871 919 928 1148 1741
Size: 12 319 303 237 48 9 220 593
Cuts at: 1741 1866
Size: 125
Fragments arranged by size:
593 319 303 237 220 125 48 12
9
Bpu10I CC'TnA_GC
Cuts at: 0 128 352 563 1866
Size: 128 224 211 1303
Fragments arranged by size:
1303 224 211 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 484 652 1359 1766 1866
Size: 484 168 707 407 100
Fragments arranged by size:
707 484 407 168 100
BsaHI Gr'CG_yC
Cuts at: 0 1817 1866
Size: 1817 49
BsaWI w'CCGG_w
Cuts at: 0 97 1866
Size: 97 1769
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 1126 1156 1866
Size: 391 30 705 30 710
Fragments arranged by size:
710 705 391 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 1267 1603 1866
Size: 148 1119 336 263
Fragments arranged by size:
1119 336 263 148
BseYI C'CCAG_C
Cuts at: 0 558 1866
Size: 558 1308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1642 1688 1866
Size: 400 1242 46 178
Fragments arranged by size:
1242 400 178 46
BsiHKAI G_wGCw'C
Cuts at: 0 795 1866
Size: 795 1071
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1076 1866
Size: 1076 790
Bsp1286I G_dGCh'C
Cuts at: 0 28 149 158 179 335 353 795 1172
Size: 28 121 9 21 156 18 442 377
Cuts at: 1172 1230 1404 1633 1866
Size: 58 174 229 233
Fragments arranged by size:
442 377 233 229 174 156 121 58
28 21 18 9
BspHI T'CATG_A
Cuts at: 0 696 1866
Size: 696 1170
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 229 1046 1115 1376 1421 1667 1866
Size: 229 817 69 261 45 246 199
Fragments arranged by size:
817 261 246 229 199 69 45
BsrFI r'CCGG_y
Cuts at: 0 62 1866
Size: 62 1804
BsrGI T'GTAC_A
Cuts at: 0 743 1866
Size: 743 1123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 21 36 1866
Size: 21 15 1830
Fragments arranged by size:
1830 21 15
BstYI r'GATC_y
Cuts at: 0 525 1796 1866
Size: 525 1271 70
Fragments arranged by size:
1271 525 70
Bsu36I CC'TnA_GG
Cuts at: 0 238 708 1181 1463 1866
Size: 238 470 473 282 403
Fragments arranged by size:
473 470 403 282 238
BtgI C'CryG_G
Cuts at: 0 721 1866
Size: 721 1145
BtsI GCAGTG_nn'
Cuts at: 0 1522 1700 1866
Size: 1522 178 166
Fragments arranged by size:
1522 178 166
EaeI y'GGCC_r
Cuts at: 0 1581 1866
Size: 1581 285
EarI CTCTTCn'nnn_
Cuts at: 0 1453 1669 1866
Size: 1453 216 197
Fragments arranged by size:
1453 216 197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 331 634 871 919 928 1148 1357
Size: 12 319 303 237 48 9 220 209
Cuts at: 1357 1648 1693 1741 1866
Size: 291 45 48 125
Fragments arranged by size:
319 303 291 237 220 209 125 48
48 45 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 820 1372 1866
Size: 820 552 494
Fragments arranged by size:
820 552 494
EcoO109I rG'GnC_Cy
Cuts at: 0 331 471 758 838 1168 1169 1866
Size: 331 140 287 80 330 1 697
Fragments arranged by size:
697 331 330 287 140 80 1
EcoRV GAT'ATC
Cuts at: 0 285 1866
Size: 285 1581
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 765 797 988 1020 1668 1700
Size: 391 32 342 32 191 32 648 32
Cuts at: 1700 1866
Size: 166
Fragments arranged by size:
648 391 342 191 166 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 246 1866
Size: 246 1620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1866
Size: 320 1546
MslI CAynn'nnrTG
Cuts at: 0 19 1710 1748 1866
Size: 19 1691 38 118
Fragments arranged by size:
1691 118 38 19
MspA1I CmG'CkG
Cuts at: 0 32 187 291 562 1201 1580 1866
Size: 32 155 104 271 639 379 286
Fragments arranged by size:
639 379 286 271 155 104 32
PasI CC'CwG_GG
Cuts at: 0 255 600 1866
Size: 255 345 1266
Fragments arranged by size:
1266 345 255
PflMI CCAn_nnn'nTGG
Cuts at: 0 1410 1866
Size: 1410 456
PfoI T'CCnGG_A
Cuts at: 0 650 1866
Size: 650 1216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 335 367 1126 1158 1866
Size: 335 32 759 32 708
Fragments arranged by size:
759 708 335 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 471 758 838 1866
Size: 471 287 80 1028
Fragments arranged by size:
1028 471 287 80
PspOMI G'GGCC_C
Cuts at: 0 331 1168 1866
Size: 331 837 698
Fragments arranged by size:
837 698 331
PspXI vC'TCGA_Gb
Cuts at: 0 1438 1866
Size: 1438 428
PstI C_TGCA'G
Cuts at: 0 186 437 593 1092 1529 1866
Size: 186 251 156 499 437 337
Fragments arranged by size:
499 437 337 251 186 156
PvuII CAG'CTG
Cuts at: 0 32 187 562 1866
Size: 32 155 375 1304
Fragments arranged by size:
1304 375 155 32
SapI GCTCTTCn'nnn_
Cuts at: 0 1453 1866
Size: 1453 413
SbfI CC_TGCA'GG
Cuts at: 0 437 593 1866
Size: 437 156 1273
Fragments arranged by size:
1273 437 156
SfcI C'TryA_G
Cuts at: 0 182 433 589 1063 1088 1525 1866
Size: 182 251 156 474 25 437 341
Fragments arranged by size:
474 437 341 251 182 156 25
SmlI C'TyrA_G
Cuts at: 0 1438 1866
Size: 1438 428
StuI AGG'CCT
Cuts at: 0 713 856 925 1384 1866
Size: 713 143 69 459 482
Fragments arranged by size:
713 482 459 143 69
StyI C'CwwG_G
Cuts at: 0 80 203 642 1138 1325 1866
Size: 80 123 439 496 187 541
Fragments arranged by size:
541 496 439 187 123 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1378 1387 1866
Size: 1378 9 479
Fragments arranged by size:
1378 479 9
TatI w'GTAC_w
Cuts at: 0 743 800 1866
Size: 743 57 1066
Fragments arranged by size:
1066 743 57
Tth111I GACn'n_nGTC
Cuts at: 0 370 1866
Size: 370 1496
XhoI C'TCGA_G
Cuts at: 0 1438 1866
Size: 1438 428
XmnI GAAnn'nnTTC
Cuts at: 0 1832 1866
Size: 1832 34
Enzymes that do cut:
AarI AcuI AleI AlfI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbvCI BcgI BciVI BclI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrFI BsrGI BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV
Hin4I HindIII MmeI MslI MspA1I PasI PflMI PfoI
PpiI PpuMI PspOMI PspXI PstI PvuII SapI SbfI
SfcI SmlI StuI StyI TaqII TatI Tth111I XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BfrBI BglII BmgBI BmtI BpuEI BsaAI BsaBI
BsiEI BsiWI BsmI BspEI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PshAI PsiI PsrI PvuI RsrII SacI SacII SalI
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XcmI XmaI ZraI