Restriction map for
pFN21AB8369
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HindIII
HphI KpnI MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 584 GACGTC GACGT^C
AccII 275 CGCG CG^CG
AcsI 180 GAATTT [GA]^AATT[CT]
AfaI 219 GTAC GT^AC
AluI 247 AGCT AG^CT
AlwI 406 GATCC GGATC(4/5)
ApoI 180 GAATTT [GA]^AATT[CT]
Asp700I 90 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 64 GAGCAC G[AT]GC[AT]^C
AviII 442 TGCGCA TGC^GCA
BbeI 345 GGCGCC GGCGC^C
BbsI 581 GAAGAC GAAGAC(2/6)
BpmI 37 CTGGAG CTGGAG(16/14)
BpuAI 581 GAAGAC GAAGAC(2/6)
BsiHKAI 64 GAGCAC G[AT]GC[AT]^C
BsmAI 149 GAGAC GTCTC(1/5)
BsmFI 128 GGGAC GGGAC(10/14)
BspMI 462 ACCTGC ACCTGC(4/8)
BsrBI 40 GAGCGG CCGCTC(-3/-3)
BsrDI 438 GCAATG GCAATG(2/0)
BstUI 275 CGCG CG^CG
DpnII 406 GATC ^GATC
DrdI 402 GACTGATCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 274 CCGCGG C^C[GA][CT]GG
FspI 442 TGCGCA TGC^GCA
HaeII 345 GGCGCC [GA]GCGC^[CT]
HincII 120 GTTAAC GT[CT]^[GA]AC
HindII 120 GTTAAC GT[CT]^[GA]AC
HpaI 120 GTTAAC GTT^AAC
KasI 345 GGCGCC G^GCGCC
KspI 274 CCGCGG CCGC^GG
MboI 406 GATC ^GATC
MvnI 275 CGCG CG^CG
NaeI 303 GCCGGC GCC^GGC
NarI 345 GGCGCC GG^CGCC
NdeII 406 GATC ^GATC
NgoMIV 303 GCCGGC G^CCGGC
NlaIII 153 CATG CATG^
RsaI 219 GTAC GT^AC
SacII 274 CCGCGG CCGC^GG
Sau3AI 406 GATC ^GATC
SbfI 83 CCTGCAGG CCTGCA^GG
SfoI 345 GGCGCC GGC^GCC
SmaI 125 CCCGGG CCC^GGG
Sse8387I 83 CCTGCAGG CCTGCA^GG
TaqI 185 TCGA T^CGA
TspRI 142 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 185 TCGA T^CGA
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
XmnI 90 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 125 CCCGGG C^[CT]CG[GA]G
253 CCCGAG
AvaII 108 GGACC G^G[AT]CC
393 GGACC
BanII 27 GAGCCC G[GA]GC[CT]^C
359 GGGCTC
BbvI 245 GCAGC GCAGC(8/12)
326 GCAGC
BfaI 32 CTAG C^TAG
208 CTAG
BglI 52 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
317 GCCAGGATGGC
BsoBI 125 CCCGGG C^[CT]CG[GA]G
253 CCCGAG
BsrFI 303 GCCGGC [GA]^CCGG[CT]
541 GCCGGT
BstF5I 44 GGATG GGATG(2/0)
321 GGATG
Cfr10I 303 GCCGGC [GA]^CCGG[CT]
541 GCCGGT
DdeI 374 CTGAG C^T[ACGT]AG
577 CTCAG
FauI 273 CCCGC CCCGC(4/6)
329 GCGGG
FokI 44 GGATG GGATG(9/13)
321 GGATG
HgaI 49 GACGC GACGC(5/10)
558 GACGC
HinfI 195 GAATC G^A[ACGT]TC
475 GATTC
MaeI 32 CTAG C^TAG
208 CTAG
MaeII 8 ACGT A^CGT
585 ACGT
MseI 121 TTAA T^TAA
352 TTAA
MspA1I 274 CCGCGG C[AC]G^C[GT]G
327 CAGCGG
PstI 84 CTGCAG CTGCA^G
144 CTGCAG
TfiI 195 GAATC G^A[AT]TC
475 GATTC
Tru9I 121 TTAA T^TAA
352 TTAA
TseI 245 GCAGC G^C[AT]GC
326 GCAGC
Tsp509I 181 AATT ^AATT
354 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcyI BanI BcnI BmyI
BsaHI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
EarI EcoRII Fnu4HI HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
Ksp632I MboII MnlI MspI MvaI
MwoI NciI NlaIV Sau96I ScrFI
SfcI